BLASTX 7.6.2
Query= UN34938 /QuerySize=1129
(1128 letters)
Database: GenBank nr;
15,229,318 sequences; 5,219,829,378 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|22655064|gb|AAM98123.1| putative isoamylase [Arabidopsis thal... 185 1e-044
gi|15218771|ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] 184 4e-044
gi|297843142|ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidops... 167 3e-039
gi|255584716|ref|XP_002533079.1| isoamylase, putative [Ricinus c... 95 2e-017
gi|73698627|gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum] 93 6e-017
gi|225436952|ref|XP_002271798.1| PREDICTED: hypothetical protein... 90 7e-016
gi|139867055|dbj|BAF52942.1| isoamylase-type starch-debranching ... 86 8e-015
gi|27728147|gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum] 85 2e-014
gi|224149032|ref|XP_002336746.1| predicted protein [Populus tric... 79 1e-012
gi|41687960|dbj|BAD08581.1| 85kDa isoamylase [Hordeum vulgare su... 64 4e-008
gi|229610875|emb|CAX51369.1| isoamylase [Hordeum vulgare subsp. ... 64 4e-008
gi|326498607|dbj|BAK02289.1| predicted protein [Hordeum vulgare ... 64 4e-008
gi|294462564|gb|ADE76828.1| unknown [Picea sitchensis] 61 3e-007
gi|162463513|ref|NP_001105666.1| isoamylase-type starch debranch... 60 8e-007
gi|195657421|gb|ACG48178.1| isoamylase-type starch debranching e... 60 8e-007
gi|51038091|gb|AAT93894.1| putative isoamylase-type starch debra... 59 1e-006
gi|125551014|gb|EAY96723.1| hypothetical protein OsI_18641 [Oryz... 59 1e-006
>gi|22655064|gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
Length = 882
Score = 185 bits (469), Expect = 1e-044
Identities = 124/237 (52%), Positives = 150/237 (63%), Gaps = 23/237 (9%)
Frame = +2
Query: 245 MAFWSTSAGIGSCCLLNNDITETWRFPST-CFSTCKNKIKRGSETLRVTRKHISSDLVTS 421
MA WS S GIGSCC LNN IT TW+FPS F+ KNKIK GSETL TRK DLVTS
Sbjct: 1 MAAWSPSVGIGSCC-LNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 59
Query: 422 ALQSYHHRLSKIRALKTSTELKEEVSTRSSQVEDPKKVTRSSYSFRTKSGALVKVKVEKK 601
ALQSY + SKI A KTS EL+E +S+R ++ +D KKVT SYSFRTK+GALVKVKVEKK
Sbjct: 60 ALQSY--QFSKICASKTSIELREALSSRRAEADDLKKVT--SYSFRTKAGALVKVKVEKK 115
Query: 602 REEKYSIMVYV-----SGDDNKSSSLVMVWGVYRSDSSSKFLALEIKAETEEETTALSPV 766
R EKYSI+VYV SGDD S LVMVWGVYRSDSS FL L+ + +++ T +
Sbjct: 116 R-EKYSILVYVSSLELSGDD--KSRLVMVWGVYRSDSSC-FLPLDFENSSQDSQTHTTET 171
Query: 767 EPTEPKNNDLFIG--FNASDQP------ENVILPSLPDGREWRRLVDTALPFPGFFS 913
+ ++L +G F+ + P ++ PDGRE DT P F+
Sbjct: 172 TFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGREMLTHRDTDFCIPVGFT 228
Score = 165 bits (417), Expect = 1e-038
Identities = 84/107 (78%), Positives = 93/107 (86%), Gaps = 4/107 (3%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWR 871
D +SKFLALEIK+E+EEE TA S EP EPK+NDLFIGFNASD PE+V+LPSLPDG +WR
Sbjct: 775 DPASKFLALEIKSESEEEETA-SLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWR 833
Query: 872 RLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFET 1012
RLVDTALPFPGFFSVEGETVVA EE + Q V+YEMKPYSCTLFET
Sbjct: 834 RLVDTALPFPGFFSVEGETVVA---EEPLQQLVVYEMKPYSCTLFET 877
>gi|15218771|ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana]
Length = 882
Score = 184 bits (465), Expect = 4e-044
Identities = 123/237 (51%), Positives = 150/237 (63%), Gaps = 23/237 (9%)
Frame = +2
Query: 245 MAFWSTSAGIGSCCLLNNDITETWRFPST-CFSTCKNKIKRGSETLRVTRKHISSDLVTS 421
MA WS S GIGSCC LNN IT TW+FPS F+ KNKIK GSETL TRK DLVTS
Sbjct: 1 MAAWSPSVGIGSCC-LNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTS 59
Query: 422 ALQSYHHRLSKIRALKTSTELKEEVSTRSSQVEDPKKVTRSSYSFRTKSGALVKVKVEKK 601
ALQSY + SKI A KTS EL+E +S+R ++ +D KKVT SYSFRTK+GALVKVKVEKK
Sbjct: 60 ALQSY--QFSKICASKTSIELREALSSRRAEADDLKKVT--SYSFRTKAGALVKVKVEKK 115
Query: 602 REEKYSIMVYV-----SGDDNKSSSLVMVWGVYRSDSSSKFLALEIKAETEEETTALSPV 766
R EKYSI+VYV SGDD S LVMVWGVYRSDSS FL L+ + +++ T +
Sbjct: 116 R-EKYSILVYVSSLELSGDD--KSRLVMVWGVYRSDSSC-FLPLDFENSSQDSQTHTTET 171
Query: 767 EPTEPKNNDLFIG--FNASDQP------ENVILPSLPDGREWRRLVDTALPFPGFFS 913
+ ++L +G F+ + P ++ PDG+E DT P F+
Sbjct: 172 TFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFT 228
Score = 165 bits (417), Expect = 1e-038
Identities = 84/107 (78%), Positives = 93/107 (86%), Gaps = 4/107 (3%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWR 871
D +SKFLALEIK+E+EEE TA S EP EPK+NDLFIGFNASD PE+V+LPSLPDG +WR
Sbjct: 775 DPASKFLALEIKSESEEEETA-SLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWR 833
Query: 872 RLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFET 1012
RLVDTALPFPGFFSVEGETVVA EE + Q V+YEMKPYSCTLFET
Sbjct: 834 RLVDTALPFPGFFSVEGETVVA---EEPLQQLVVYEMKPYSCTLFET 877
>gi|297843142|ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata
subsp. lyrata]
Length = 872
Score = 167 bits (422), Expect = 3e-039
Identities = 112/217 (51%), Positives = 138/217 (63%), Gaps = 21/217 (9%)
Frame = +2
Query: 302 ITETWRFPSTCFSTCKNKIKRGSETLRVTRKHISSDLVTSALQSYHHRLSKIRALKTSTE 481
+T TW+FPS F T KNKIK GSETL TRK DLVTSAL+SY +LSKI A KTS E
Sbjct: 9 VTRTWKFPSARFFTGKNKIKLGSETLMFTRKRFMGDLVTSALKSY--QLSKICASKTSIE 66
Query: 482 LKEEVSTRSSQVEDPKKVTRSSYSFRTKSGALVKVKVEKKREEKYSIMVYVS-----GDD 646
L+E +ST+ +QV+D KKVT SYSFRTKSGALVKVKVE+KR EKYSIMVYVS GDD
Sbjct: 67 LREALSTKRAQVDDLKKVT--SYSFRTKSGALVKVKVEQKR-EKYSIMVYVSSLELTGDD 123
Query: 647 NKSSSLVMVWGVYRSDSSSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIG--FNASD 820
S LVMVWGVYRSDSS FL L+ + +++ T + + ++L +G F+ +
Sbjct: 124 --KSRLVMVWGVYRSDSSC-FLPLDFENSSQDSQTHTTETPFVKSSLSELMLGLEFDGKE 180
Query: 821 QP------ENVILPSLPDGREWRRLVDTALPFPGFFS 913
P ++ PDG+E DT P F+
Sbjct: 181 SPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFT 217
Score = 167 bits (421), Expect = 4e-039
Identities = 84/107 (78%), Positives = 95/107 (88%), Gaps = 3/107 (2%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWR 871
D +SKFLALEIK+E+EEE TA S VEPTEPK+NDLFIGFNASD PE+VILPSLPDG +WR
Sbjct: 764 DPTSKFLALEIKSESEEEETA-SLVEPTEPKSNDLFIGFNASDHPESVILPSLPDGSKWR 822
Query: 872 RLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFET 1012
RLVDTALPFPGFFSVEGETVV EE++ Q ++YEM+PYSCTLFET
Sbjct: 823 RLVDTALPFPGFFSVEGETVVV--AEEQLQQLIVYEMEPYSCTLFET 867
>gi|255584716|ref|XP_002533079.1| isoamylase, putative [Ricinus communis]
Length = 872
Score = 95 bits (234), Expect = 2e-017
Identities = 52/108 (48%), Positives = 69/108 (63%), Gaps = 13/108 (12%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEETTALSPVEPTEPKN--NDLFIGFNASDQPENVILPSLPDGRE 865
D + KFLA+ +K + E + +EP N DLF+ FNA+ E+VILP +P+G
Sbjct: 770 DPTCKFLAMTLKIDKAESQLS------SEPSNIKGDLFMAFNAAGHAESVILPPVPEGMI 823
Query: 866 WRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFE 1009
WRRLVDTALPFPGFFS +GE VV E++ + Y+M +SCTLFE
Sbjct: 824 WRRLVDTALPFPGFFSEDGEPVV-----EQIAGLIAYKMNSHSCTLFE 866
>gi|73698627|gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]
Length = 857
Score = 93 bits (230), Expect = 6e-017
Identities = 52/108 (48%), Positives = 66/108 (61%), Gaps = 13/108 (12%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEE--ETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGRE 865
D S KFLA+ +KAE E E++ S + DLF+ FNA D PE V+LP P+G
Sbjct: 755 DPSCKFLAMTLKAERNEHQESSVSSDI------LGDLFVAFNADDCPETVVLPLPPEGMS 808
Query: 866 WRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFE 1009
W R++DTALPFPGFF G+ V+ E+M+ YEMK YSC LFE
Sbjct: 809 WYRIIDTALPFPGFFLNNGDLVL-----EQMSGLSTYEMKSYSCILFE 851
>gi|225436952|ref|XP_002271798.1| PREDICTED: hypothetical protein [Vitis
vinifera]
Length = 882
Score = 90 bits (221), Expect = 7e-016
Identities = 51/106 (48%), Positives = 66/106 (62%), Gaps = 9/106 (8%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWR 871
D SSKFLA+ +KAE E P E + K DLFI FN +D+ VILP P G W
Sbjct: 780 DPSSKFLAMTLKAENME---GQLPSESSSIK-GDLFIAFNTADRSVKVILPPPPTGMVWH 835
Query: 872 RLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFE 1009
RLVDTALPFPGFF+ +GE ++ ++ + V Y+M+ +SC LFE
Sbjct: 836 RLVDTALPFPGFFTADGEAIL-----KKKSGLVTYKMESHSCALFE 876
>gi|139867055|dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2
[Phaseolus vulgaris]
Length = 865
Score = 86 bits (212), Expect = 8e-015
Identities = 48/107 (44%), Positives = 63/107 (58%), Gaps = 11/107 (10%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWR 871
D S KFLA+ +K+ E LS + + D+FI N +D+PE+ +LP P+G W
Sbjct: 763 DPSCKFLAMTLKS----EVAVLSESSVSSDISGDIFIALNVADEPESTVLPLPPEGMSWY 818
Query: 872 RLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV-MYEMKPYSCTLFE 1009
RLVDT+LPFPGFFS GE V EM + Y+MK +SC LFE
Sbjct: 819 RLVDTSLPFPGFFSSSGEIV------PEMKAGLSTYKMKSHSCALFE 859
>gi|27728147|gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
Length = 878
Score = 85 bits (209), Expect = 2e-014
Identities = 50/104 (48%), Positives = 61/104 (58%), Gaps = 14/104 (13%)
Frame = +2
Query: 698 SSKFLALEIKAETEEETTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGREWRRL 877
SSKFLA+ +KA+ E T +S + DLF+ FN + E VILP P W RL
Sbjct: 766 SSKFLAMTLKADAEVSQTLVSDIV------GDLFVAFNGAGDSEIVILPPPPTDMVWHRL 819
Query: 878 VDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSCTLFE 1009
VDTALPFPGFF +G V E+E+ V YEMK +SC LFE
Sbjct: 820 VDTALPFPGFFDEKGTPV-----EDEL---VAYEMKSHSCLLFE 855
>gi|224149032|ref|XP_002336746.1| predicted protein [Populus trichocarpa]
Length = 283
Score = 79 bits (193), Expect = 1e-012
Identities = 47/104 (45%), Positives = 61/104 (58%), Gaps = 18/104 (17%)
Frame = +2
Query: 692 DSSSKFLALEIKAETEEE--TTALSPVEPTEPKNNDLFIGFNASDQPENVILPSLPDGRE 865
D S KFLA+ +K + ++ S VE D+FI FNA+ + E+V LP +P+G
Sbjct: 186 DPSCKFLAMTLKVDNPGSGLSSESSHVE------GDMFIAFNAAGRSESVTLPEVPEGMA 239
Query: 866 WRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPVMYEMKPYSC 997
W RLVDTALPFPGFFS + E V+ QP YEMK +SC
Sbjct: 240 WHRLVDTALPFPGFFSNDSEPVI--------RQP--YEMKSHSC 273
>gi|41687960|dbj|BAD08581.1| 85kDa isoamylase [Hordeum vulgare subsp. vulgare]
Length = 798
Score = 64 bits (154), Expect = 4e-008
Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 5/79 (6%)
Frame = +2
Query: 776 EPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEE 955
E DL+I FNAS++P + LP+ +G W RLVDT+L FP FF+ E E ++
Sbjct: 716 ELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLTFPSFFTTESNL-----EVQQ 770
Query: 956 MNQPVMYEMKPYSCTLFET 1012
+ YE+K +SC LFE+
Sbjct: 771 VLGYSSYEVKAHSCVLFES 789
>gi|229610875|emb|CAX51369.1| isoamylase [Hordeum vulgare subsp. vulgare]
Length = 798
Score = 64 bits (154), Expect = 4e-008
Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 5/79 (6%)
Frame = +2
Query: 776 EPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEE 955
E DL+I FNAS++P + LP+ +G W RLVDT+L FP FF+ E E ++
Sbjct: 716 ELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLTFPSFFTTESNL-----EVQQ 770
Query: 956 MNQPVMYEMKPYSCTLFET 1012
+ YE+K +SC LFE+
Sbjct: 771 VLGYSSYEVKAHSCVLFES 789
>gi|326498607|dbj|BAK02289.1| predicted protein [Hordeum vulgare subsp.
vulgare]
Length = 798
Score = 64 bits (154), Expect = 4e-008
Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 5/79 (6%)
Frame = +2
Query: 776 EPKNNDLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEE 955
E DL+I FNAS++P + LP+ +G W RLVDT+L FP FF+ E E ++
Sbjct: 716 ELTKGDLYICFNASEEPASATLPAPAEGSVWLRLVDTSLTFPSFFTTESNL-----EVQQ 770
Query: 956 MNQPVMYEMKPYSCTLFET 1012
+ YE+K +SC LFE+
Sbjct: 771 VLGYSSYEVKAHSCVLFES 789
>gi|294462564|gb|ADE76828.1| unknown [Picea sitchensis]
Length = 175
Score = 61 bits (147), Expect = 3e-007
Identities = 32/73 (43%), Positives = 42/73 (57%), Gaps = 5/73 (6%)
Frame = +2
Query: 791 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 970
DL+I FN+ + LP LPDG W RLVDT+LPFP FS+EG +
Sbjct: 102 DLYIAFNSQKASVSATLPQLPDGMLWHRLVDTSLPFPKTFSMEGMAT-----DMLGISFS 156
Query: 971 MYEMKPYSCTLFE 1009
Y+++P+SC LFE
Sbjct: 157 TYQIQPHSCVLFE 169
>gi|162463513|ref|NP_001105666.1| isoamylase-type starch debranching enzyme ISO2
[Zea mays]
Length = 799
Score = 60 bits (143), Expect = 8e-007
Identities = 30/74 (40%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = +2
Query: 791 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 970
DL+I FNA+++ + LP++ +G W RLVDT+L FPGFFS + E ++
Sbjct: 726 DLYICFNANEESASATLPAIAEGSMWLRLVDTSLAFPGFFSRG-----SSHETHQVLGFS 780
Query: 971 MYEMKPYSCTLFET 1012
Y++K +SC LFE+
Sbjct: 781 SYQVKAHSCVLFES 794
>gi|195657421|gb|ACG48178.1| isoamylase-type starch debranching enzyme ISO2 [Zea
mays]
Length = 799
Score = 60 bits (143), Expect = 8e-007
Identities = 30/74 (40%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = +2
Query: 791 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 970
DL+I FNA+++ + LP++ +G W RLVDT+L FPGFFS + E ++
Sbjct: 726 DLYICFNANEESASATLPAIAEGSMWLRLVDTSLAFPGFFSRG-----SSHETHQVLGFS 780
Query: 971 MYEMKPYSCTLFET 1012
Y++K +SC LFE+
Sbjct: 781 SYQVKAHSCVLFES 794
>gi|51038091|gb|AAT93894.1| putative isoamylase-type starch debranching enzyme
ISO2 [Oryza sativa Japonica Group]
Length = 800
Score = 59 bits (142), Expect = 1e-006
Identities = 29/74 (39%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = +2
Query: 791 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 970
DL+I FNA+++ + LP+L +G W RLVDT+L FPGFF+ E V +++
Sbjct: 727 DLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKV-----QQVPGLS 781
Query: 971 MYEMKPYSCTLFET 1012
Y ++ ++C LFE+
Sbjct: 782 SYHVEAHTCVLFES 795
>gi|125551014|gb|EAY96723.1| hypothetical protein OsI_18641 [Oryza sativa Indica
Group]
Length = 840
Score = 59 bits (142), Expect = 1e-006
Identities = 29/74 (39%), Positives = 46/74 (62%), Gaps = 5/74 (6%)
Frame = +2
Query: 791 DLFIGFNASDQPENVILPSLPDGREWRRLVDTALPFPGFFSVEGETVVAEEEEEEMNQPV 970
DL+I FNA+++ + LP+L +G W RLVDT+L FPGFF+ E V +++
Sbjct: 767 DLYICFNANEESVSAALPALAEGSVWLRLVDTSLAFPGFFATESNPKV-----QQVPGLS 821
Query: 971 MYEMKPYSCTLFET 1012
Y ++ ++C LFE+
Sbjct: 822 SYHVEAHTCVLFES 835
Database: GenBank nr
Posted date: Thu Sep 08 23:06:31 2011
Number of letters in database: 5,219,829,378
Number of sequences in database: 15,229,318
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,948,232,901,940
Number of Sequences: 15229318
Number of Extensions: 3948232901940
Number of Successful Extensions: 930432217
Number of sequences better than 0.0: 0
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