BLASTX 7.6.2
Query= UN35925 /QuerySize=900
(899 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SP... 130 1e-029
sp|Q9NRA0|SPHK2_HUMAN Sphingosine kinase 2 OS=Homo sapiens GN=SP... 129 2e-029
sp|Q86KF9|SPHKA_DICDI Sphingosine kinase A OS=Dictyostelium disc... 129 2e-029
sp|Q9JIA7|SPHK2_MOUSE Sphingosine kinase 2 OS=Mus musculus GN=Sp... 122 5e-027
sp|Q91V26|SPHK1_RAT Sphingosine kinase 1 OS=Rattus norvegicus GN... 120 2e-026
sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sp... 119 3e-026
sp|O14159|LCB4_SCHPO Sphingoid long chain base kinase 4 OS=Schiz... 117 1e-025
sp|Q6B516|SPHKB_DICDI Sphingosine kinase B OS=Dictyostelium disc... 110 2e-023
sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens GN=CERK PE... 109 5e-023
sp|Q8K4Q7|CERK1_MOUSE Ceramide kinase OS=Mus musculus GN=Cerk PE... 101 1e-020
sp|Q06147|LCB5_YEAST Sphingoid long chain base kinase 5 OS=Sacch... 98 8e-020
sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1 OS=Ara... 96 2e-019
sp|Q12246|LCB4_YEAST Sphingoid long chain base kinase 4 OS=Sacch... 90 2e-017
sp|Q10123|YSM3_CAEEL Uncharacterized protein F52C9.3 OS=Caenorha... 62 5e-009
>sp|Q9NYA1|SPHK1_HUMAN Sphingosine kinase 1 OS=Homo sapiens GN=SPHK1 PE=1 SV=1
Length = 384
Score = 130 bits (326), Expect = 1e-029
Identities = 68/172 (39%), Positives = 108/172 (62%), Gaps = 6/172 (3%)
Frame = +1
Query: 373 LGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVS 552
L RP R+LV +NP GGK A+++F V+PL +A+I + T+ + HA+E+VRS ++
Sbjct: 11 LPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELG 70
Query: 553 KYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSLLDPVGLKCCA 732
++D +V +SGDG++ EVVNGL+E DW T + P+ +PAG+GN + SL G +
Sbjct: 71 RWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVT 130
Query: 733 N-----SATISIIRGHTRSLDVATISQGT-TKFFSVLMLAWGLIADIDIESE 870
N + T+ + R +++ ++ + + FSVL LAWG IAD+D+ESE
Sbjct: 131 NEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESE 182
>sp|Q9NRA0|SPHK2_HUMAN Sphingosine kinase 2 OS=Homo sapiens GN=SPHK2 PE=1 SV=2
Length = 654
Score = 129 bits (324), Expect = 2e-029
Identities = 68/175 (38%), Positives = 113/175 (64%), Gaps = 8/175 (4%)
Frame = +1
Query: 367 DSLGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMD 546
D L RP RLL+ VNPFGG+ A + + V P+ +A + ++ T+ Q HA+E+V+ +
Sbjct: 175 DLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLS 234
Query: 547 VSKYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSL-----LDP 711
+S++DGIV VSGDG+L EV+NGLL+ DW K+P+G++P G+GN + ++ +P
Sbjct: 235 LSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEP 294
Query: 712 -VGLKCCANSATISIIRGHTRSLDVATISQGT-TKFFSVLMLAWGLIADIDIESE 870
+GL N ++ + RG LD+ +++ + ++ FS L +AWG ++D+DI+SE
Sbjct: 295 ALGLDLLLN-CSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSE 348
>sp|Q86KF9|SPHKA_DICDI Sphingosine kinase A OS=Dictyostelium discoideum GN=sgkA
PE=2 SV=2
Length = 624
Score = 129 bits (324), Expect = 2e-029
Identities = 74/176 (42%), Positives = 109/176 (61%), Gaps = 14/176 (7%)
Frame = +1
Query: 361 YLDSL----GRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKE 528
+LD+L R +R+ V +NP GKK + IF K++ LF+D+ I + TK HAK+
Sbjct: 169 FLDTLPMGNPRERRIRVILNPKSGKKMSDSIF-KDINELFKDSKIFVKKTVTKGPDHAKK 227
Query: 529 MVRSMDVSKYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKS--L 702
+ ++ KYD IV +SGDG+ E +NGLL D+ K+P+ +IP GTGNG+ S L
Sbjct: 228 IGYKFNLKKYDTIVFISGDGLFHEFINGLLSRTDFEQARKIPLALIPGGTGNGIACSIGL 287
Query: 703 LDPVGLKCCANSATISIIRGHTRSLDVATISQGTTKFFSVLMLAWGLIADIDIESE 870
DP+ S +++IRG T+ LDV+ I QG K+ S+L L WG+++D+DIESE
Sbjct: 288 QDPM-------SCALAVIRGFTKPLDVSVIQQGDKKWCSILSLTWGIVSDVDIESE 336
>sp|Q9JIA7|SPHK2_MOUSE Sphingosine kinase 2 OS=Mus musculus GN=Sphk2 PE=1 SV=2
Length = 617
Score = 122 bits (304), Expect = 5e-027
Identities = 63/172 (36%), Positives = 107/172 (62%), Gaps = 6/172 (3%)
Frame = +1
Query: 373 LGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVS 552
L R RLL+ VNPFGG+ A + V P+ +A + ++ T+ Q HA+E+V+ + +S
Sbjct: 142 LPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLS 201
Query: 553 KYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSLLDPVGLKCCA 732
+++GIV VSGDG+L EV+NGLL+ DW ++PIGV+P G+GN + ++ G +
Sbjct: 202 EWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVV 261
Query: 733 N-----SATISIIRGHTRSLDVATISQGT-TKFFSVLMLAWGLIADIDIESE 870
+ ++ + RG + LD+ +++ + ++ FS L +AWG ++D+DI SE
Sbjct: 262 GVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSE 313
>sp|Q91V26|SPHK1_RAT Sphingosine kinase 1 OS=Rattus norvegicus GN=Sphk1 PE=1
SV=1
Length = 383
Score = 120 bits (299), Expect = 2e-026
Identities = 61/172 (35%), Positives = 105/172 (61%), Gaps = 6/172 (3%)
Frame = +1
Query: 373 LGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVS 552
L RP R+LV +NP GGK A+K+F+ V+PL E+A++ + T+ Q HA+E+V + ++
Sbjct: 11 LPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELG 70
Query: 553 KYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSLLDPVGLKCCA 732
+D + +SGDG++ EVVNGL+E DW + + P+ +P G+GN + SL G +
Sbjct: 71 HWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVT 130
Query: 733 N-----SATISIIRGHTRSLDVATISQGT-TKFFSVLMLAWGLIADIDIESE 870
N + T+ + +++ ++ + + +SVL L+WG +AD+D+ESE
Sbjct: 131 NEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESE 182
>sp|Q8CI15|SPHK1_MOUSE Sphingosine kinase 1 OS=Mus musculus GN=Sphk1 PE=1 SV=1
Length = 382
Score = 119 bits (297), Expect = 3e-026
Identities = 60/172 (34%), Positives = 105/172 (61%), Gaps = 6/172 (3%)
Frame = +1
Query: 373 LGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVS 552
L RP R+LV +NP GGK A+++F+ V+P E+A+I + T+ + HA+E+V + ++
Sbjct: 11 LPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEELG 70
Query: 553 KYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSLLDPVGLKCCA 732
+D + +SGDG++ EVVNGL+E DW T + P+ +P G+GN + S+ G +
Sbjct: 71 HWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVT 130
Query: 733 N-----SATISIIRGHTRSLDVATISQGT-TKFFSVLMLAWGLIADIDIESE 870
N + T+ + R +++ ++ + + +SVL L+WG +AD+D+ESE
Sbjct: 131 NEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESE 182
>sp|O14159|LCB4_SCHPO Sphingoid long chain base kinase 4 OS=Schizosaccharomyces
pombe GN=lcb4 PE=2 SV=1
Length = 458
Score = 117 bits (293), Expect = 1e-025
Identities = 69/188 (36%), Positives = 103/188 (54%), Gaps = 19/188 (10%)
Frame = +1
Query: 334 NRFC-FRLRRYLDSLGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKY 510
++FC + L + R +R +VF+NP GGK A I+E E +P+F A +V T+
Sbjct: 88 DQFCEYLLDVAYKGIKRSRRFIVFINPHGGKGKAKHIWESEAEPVFSSAHSICEVVLTRR 147
Query: 511 QLHAKEMVRSMDVSKYDGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGN-- 684
+ HAK + +++DV YDGI+ V GDG+ EV+NGL E D+ FKLP+ +IP G+GN
Sbjct: 148 KDHAKSIAKNLDVGSYDGILSVGGDGLFHEVINGLGERDDYLEAFKLPVCMIPGGSGNAF 207
Query: 685 -----GMIKSLLDPVGLKCCANSATISIIRGHTRSLDVATISQGTTKFFSVLMLAWGLIA 849
G +K L + I++G S D+ T Q K +S L +G+IA
Sbjct: 208 SYNATGQLKPAL-----------TALEILKGRPTSFDLMTFEQKGKKAYSFLTANYGIIA 256
Query: 850 DIDIESES 873
D DI +E+
Sbjct: 257 DCDIGTEN 264
>sp|Q6B516|SPHKB_DICDI Sphingosine kinase B OS=Dictyostelium discoideum GN=sgkB
PE=2 SV=1
Length = 760
Score = 110 bits (274), Expect = 2e-023
Identities = 69/185 (37%), Positives = 109/185 (58%), Gaps = 15/185 (8%)
Frame = +1
Query: 292 RRDFVFE-PFSEDARNRFCFRLRRYLDSL--GRPK--RLLVFVNPFGGKKSAIKIFEKEV 456
R+ + F+ ++D+ N + +L+SL G PK ++ + +NP GKK + IF KEV
Sbjct: 214 RKSYTFQFKSNQDSLNFYSNIQSTFLNSLPRGNPKNRKIRILINPKSGKKESHNIF-KEV 272
Query: 457 KPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVSKYDGIVCVSGDGVLVEVVNGLLEGADWR 636
+ LF+D+ I++ + T HAK++ ++ KYD +V +SGDG+L E +NGLL D+
Sbjct: 273 EQLFKDSGIKMKLTVTMEPEHAKKIGFKSNIYKYDTVVFISGDGLLHEFINGLLSREDYE 332
Query: 637 TVFKLPIGVIPAGTGNGMIKS--LLDPVGLKCCANSATISIIRGHTRSLDVATISQGTTK 810
K+P+ +IPAGTGNG+ S L DP+ SA ++I+RG T+ LDV + Q T
Sbjct: 333 DAKKIPLALIPAGTGNGLANSIGLQDPM-------SAALAILRGFTKPLDVCIVQQPTVT 385
Query: 811 FFSVL 825
V+
Sbjct: 386 TIPVV 390
>sp|Q8TCT0|CERK1_HUMAN Ceramide kinase OS=Homo sapiens GN=CERK PE=1 SV=1
Length = 537
Score = 109 bits (270), Expect = 5e-023
Identities = 71/187 (37%), Positives = 108/187 (57%), Gaps = 18/187 (9%)
Frame = +1
Query: 352 LRRYLDSL-GRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKE 528
LR L+ L RPK LLVF+NPFGGK +I+E++V PLF A I D+ T++ AKE
Sbjct: 119 LREMLEKLTSRPKHLLVFINPFGGKGQGKRIYERKVAPLFTLASITTDIIVTEHANQAKE 178
Query: 529 MVRSMDVSKYDGIVCVSGDGVLVEVVNGLL------EGADW---RTVF---KLPIGVIPA 672
+ +++ KYDGIVCV GDG+ EV++GL+ G D R V L IG+IPA
Sbjct: 179 TLYEINIDKYDGIVCVGGDGMFSEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPA 238
Query: 673 GTGNGMIKSLLDPVGLKCCANSATISIIRGHTRSLDVATISQGTTKF-FSVLMLAWGLIA 849
G+ + + S + A ++ + I+ G + ++DV+++ +T +SV +L +G
Sbjct: 239 GSTDCVCYSTVGTSD----AETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYG 294
Query: 850 DIDIESE 870
DI +SE
Sbjct: 295 DIIKDSE 301
>sp|Q8K4Q7|CERK1_MOUSE Ceramide kinase OS=Mus musculus GN=Cerk PE=2 SV=1
Length = 531
Score = 101 bits (249), Expect = 1e-020
Identities = 73/187 (39%), Positives = 105/187 (56%), Gaps = 18/187 (9%)
Frame = +1
Query: 352 LRRYLDSL-GRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKE 528
LR L+SL RPK LLVF+NPFGGK +I+EK V PLF A I ++ T++ AKE
Sbjct: 119 LRGLLESLTSRPKHLLVFINPFGGKGQGKRIYEKTVAPLFTLASITTEIIITEHANQAKE 178
Query: 529 MVRSMDVSKYDGIVCVSGDGVLVEVVNGLL------EGAD---WRTVF---KLPIGVIPA 672
+ ++ YDGIVCV GDG+ EV++G++ G D R V L IG+IPA
Sbjct: 179 TLYEINTDSYDGIVCVGGDGMFSEVLHGVIGRTQQSAGIDPNHPRAVLVPSTLRIGIIPA 238
Query: 673 GTGNGMIKSLLDPVGLKCCANSATISIIRGHTRSLDVATISQGTTKF-FSVLMLAWGLIA 849
G+ + + S VG SA + II G + ++DV+++ T +SV +L +G
Sbjct: 239 GSTDCVCYS---TVGTNDAETSA-LHIIIGDSLAIDVSSVHYHNTLLRYSVSLLGYGFYG 294
Query: 850 DIDIESE 870
D+ +SE
Sbjct: 295 DLIKDSE 301
>sp|Q06147|LCB5_YEAST Sphingoid long chain base kinase 5 OS=Saccharomyces
cerevisiae GN=LCB5 PE=1 SV=1
Length = 687
Score = 98 bits (242), Expect = 8e-020
Identities = 65/178 (36%), Positives = 92/178 (51%), Gaps = 9/178 (5%)
Frame = +1
Query: 352 LRRYLDSLGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEM 531
L+R + R K + V +NPFGGK A K+F + KPL + ++V TKY HA E+
Sbjct: 258 LKRSYKNTRRNKSIFVIINPFGGKGKAKKLFMTKAKPLLLASRCSIEVVYTKYPGHAIEI 317
Query: 532 VRSMDVSKYDGIVCVSGDGVLVEVVNGLLEGADWRTVF-KLPIGVIPAGTGNGMIKSLLD 708
R MD+ KYD I C SGDG+ EV+NGL + D F + I IP G+GN M S
Sbjct: 318 AREMDIDKYDTIACASGDGIPHEVINGLYQRPDHVKAFNNIAITEIPCGSGNAMSVS--- 374
Query: 709 PVGLKCCANSATISIIRGHTRSLDVATISQGT----TKFFSVLMLAWGLIADIDIESE 870
+ +T+ +I+ +D+ SQ + S L +GLIA+ DI +E
Sbjct: 375 -CHWTNNPSYSTLCLIKSIETRIDLMCCSQPSYAREHPKLSFLSQTYGLIAETDINTE 431
>sp|Q9LRB0|LCBK1_ARATH Sphingoid long-chain bases kinase 1 OS=Arabidopsis
thaliana GN=LCBK1 PE=1 SV=1
Length = 763
Score = 96 bits (238), Expect = 2e-019
Identities = 54/162 (33%), Positives = 98/162 (60%), Gaps = 13/162 (8%)
Frame = +1
Query: 388 RLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVSK-YDG 564
++LV +NP G +IK+F V+P+F+ A I+++V T HA+E+ ++D++ DG
Sbjct: 249 KMLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVDINLCSDG 308
Query: 565 IVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSLL---DPVGLKCCAN 735
I+CV GDG++ EV+NGLL ++ + +PIG++PAG+ N ++ ++L DP+
Sbjct: 309 IICVGGDGIINEVLNGLLTRSNPKEGVSIPIGIVPAGSDNSLVWTVLGVRDPI------- 361
Query: 736 SATISIIRGHTRSLDVATISQGTTKF--FSVLMLAWGLIADI 855
SA +SI++G + DV + T F + + +G ++D+
Sbjct: 362 SAALSIVKGGLTATDVFAVEWIHTGIIHFGMTVSYYGFVSDV 403
>sp|Q12246|LCB4_YEAST Sphingoid long chain base kinase 4 OS=Saccharomyces
cerevisiae GN=LCB4 PE=1 SV=1
Length = 624
Score = 90 bits (222), Expect = 2e-017
Identities = 55/178 (30%), Positives = 93/178 (52%), Gaps = 9/178 (5%)
Frame = +1
Query: 352 LRRYLDSLGRPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEM 531
L + ++ R + +LV +NP GGK +A +F + +P+ ++ ++++ TKY HA ++
Sbjct: 216 LEKSYENSKRNRSILVIINPHGGKGTAKNLFLTKARPILVESGCKIEIAYTKYARHAIDI 275
Query: 532 VRSMDVSKYDGIVCVSGDGVLVEVVNGLLEGADWRTVF-KLPIGVIPAGTGNGMIKSLLD 708
+ +D+SKYD I C SGDG+ EV+NGL D F KL + +P G+GN M S
Sbjct: 276 AKDLDISKYDTIACASGDGIPYEVINGLYRRPDRVDAFNKLAVTQLPCGSGNAMSIS--- 332
Query: 709 PVGLKCCANSATISIIRGHTRSLDVATISQ----GTTKFFSVLMLAWGLIADIDIESE 870
+ A + +++ +D+ SQ S L +G+IA+ DI +E
Sbjct: 333 -CHWTNNPSYAALCLVKSIETRIDLMCCSQPSYMNEWPRLSFLSQTYGVIAESDINTE 389
>sp|Q10123|YSM3_CAEEL Uncharacterized protein F52C9.3 OS=Caenorhabditis elegans
GN=F52C9.3 PE=2 SV=2
Length = 439
Score = 62 bits (149), Expect = 5e-009
Identities = 37/112 (33%), Positives = 53/112 (47%), Gaps = 3/112 (2%)
Frame = +1
Query: 379 RPKRLLVFVNPFGGKKSAIKIFEKEVKPLFEDADIQLDVQ*TKYQLHAKEMVRSMDVSKY 558
RPKR+ V VN G + F K PLF A +Q+DV Q + + ++D +
Sbjct: 66 RPKRVFVLVNVEGNSRGCFDQFNKNALPLFHLAGVQVDVVKADNQAQLEALAGAVDTQEA 125
Query: 559 DGIVCVSGDGVLVEVVNGLLEGADWRTVFKLPIGVIPAGTGNGMIKSLLDPV 714
D + V GDG + VV G+ R +LP+G P G N +K +L V
Sbjct: 126 DILYVVGGDGTIGTVVTGIFRN---REKAQLPVGFYPGGYDNLWLKRMLPSV 174
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 142,442,557,381
Number of Sequences: 518415
Number of Extensions: 142442557381
Number of Successful Extensions: 892588021
Number of sequences better than 0.0: 0
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