Library    |     Search    |     Batch query    |     SNP    |     SSR  

SwissProt blast output of UN38876


BLASTX 7.6.2

Query= UN38876 /QuerySize=781
        (780 letters)

Database: UniProt/SwissProt;
          518,415 sequences; 182,829,261 total letters
                                                                  Score    E
Sequences producing significant alignments:                       (bits) Value

sp|Q9C5I3|ERF76_ARATH Ethylene-responsive transcription factor 1...    198   5e-050
sp|O80340|ERF78_ARATH Ethylene-responsive transcription factor 4...    127   1e-028
sp|Q40477|ERF4_TOBAC Ethylene-responsive transcription factor 4 ...    124   6e-028
sp|Q9LW49|ERF4_NICSY Ethylene-responsive transcription factor 4 ...    123   1e-027
sp|Q9ZWA2|ERF77_ARATH Ethylene-responsive transcription factor 1...    119   2e-026
sp|Q9MAI5|ERF79_ARATH Ethylene-responsive transcription factor 8...    116   2e-025
sp|Q94ID6|ERF81_ARATH Ethylene-responsive transcription factor 1...    113   2e-024
sp|Q9SXS8|ERF3_TOBAC Ethylene-responsive transcription factor 3 ...    112   2e-024
sp|Q9FE67|ERF80_ARATH Ethylene-responsive transcription factor 9...    110   2e-023
sp|O80339|ERF82_ARATH Ethylene-responsive transcription factor 3...    108   5e-023
sp|Q70II3|EF110_ARATH Ethylene-responsive transcription factor E...    107   1e-022
sp|Q9FH54|EF114_ARATH Ethylene-responsive transcription factor E...    107   1e-022
sp|Q7G1L2|RAP26_ARATH Ethylene-responsive transcription factor R...    106   2e-022
sp|Q9LDE4|ERF83_ARATH Ethylene-responsive transcription factor 7...    105   5e-022
sp|Q9FGF8|ABR1_ARATH Ethylene-responsive transcription factor AB...    104   9e-022
sp|Q9SZ06|EF109_ARATH Ethylene-responsive transcription factor E...    104   9e-022
sp|Q9SAD4|ESR1_ARATH Ethylene-responsive transcription factor ES...    102   3e-021
sp|Q9LY29|EF115_ARATH Ethylene-responsive transcription factor E...    101   1e-020
sp|Q9LYU3|EF113_ARATH Ethylene-responsive transcription factor E...     99   4e-020
sp|Q8H0T5|ERF73_ARATH Ethylene-responsive transcription factor E...     97   8e-020

>sp|Q9C5I3|ERF76_ARATH Ethylene-responsive transcription factor 11
        OS=Arabidopsis thaliana GN=ERF11 PE=2 SV=1

          Length = 166

 Score =  198 bits (502), Expect = 5e-050
 Identities = 111/170 (65%), Positives = 127/170 (74%), Gaps = 14/170 (8%)
 Frame = -1

Query: 717 LKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYD 538
           +KTA V TN G+      RYRGVRKRPWGRYAAEIRDP+KK+RVWLGTFDTPE AARAYD
Sbjct:   5 VKTAAVKTNEGN----GVRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYD 60

Query: 537 RAALRFRGAKAKTNFP----YTYVVDNNESPTQSSTVESSSIPDLNLG--SVGSRFPLPE 376
           + A+ FRGAKAKTNFP      + +   +S +QSSTVE SS P+LNLG  SV SRFP P+
Sbjct:  61 KRAIEFRGAKAKTNFPCYNINAHCLSLTQSLSQSSTVE-SSFPNLNLGSDSVSSRFPFPK 119

Query: 375 IQVKHGMILFGEESTRSESDSSSVVMDLSPERRSRVFLDLDLNLPPKPED 226
           IQVK GM++F E   RSESDSSSVVMD+      RV LDLDLN PP PE+
Sbjct: 120 IQVKAGMMVFDE---RSESDSSSVVMDVVRYEGRRVVLDLDLNFPPPPEN 166

>sp|O80340|ERF78_ARATH Ethylene-responsive transcription factor 4 OS=Arabidopsis
        thaliana GN=ERF4 PE=1 SV=1

          Length = 222

 Score =  127 bits (318), Expect = 1e-028
 Identities = 70/127 (55%), Positives = 83/127 (65%), Gaps = 7/127 (5%)
 Frame = -1

Query: 717 LKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYD 538
           LK     TN      KE RYRGVRKRPWGRYAAEIRDP KKTRVWLGTFDT E AARAYD
Sbjct:   6 LKPDPATTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYD 65

Query: 537 RAALRFRGAKAKTNFPYTYVVDNNE-------SPTQSSTVESSSIPDLNLGSVGSRFPLP 379
            AA  FRGAKAKTNFP    + + +       SP+QSST++ +S P L + S  +    P
Sbjct:  66 TAARDFRGAKAKTNFPTFLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPP 125

Query: 378 EIQVKHG 358
           ++++  G
Sbjct: 126 QLELSLG 132

>sp|Q40477|ERF4_TOBAC Ethylene-responsive transcription factor 4 OS=Nicotiana
        tabacum GN=ERF4 PE=1 SV=1

          Length = 225

 Score =  124 bits (311), Expect = 6e-028
 Identities = 66/97 (68%), Positives = 72/97 (74%), Gaps = 7/97 (7%)
 Frame = -1

Query: 717 LKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYD 538
           LK   V T+G    +KE  YRGVRKRPWGRYAAEIRDP KK+RVWLGTFDT E AA+AYD
Sbjct:  12 LKGGNVKTDG----VKEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYD 67

Query: 537 RAALRFRGAKAKTNFPYTYVVDNNESPTQSSTVESSS 427
            AA  FRG KAKTNFP       N+SP+ SSTVESSS
Sbjct:  68 TAAREFRGPKAKTNFPSP---TENQSPSHSSTVESSS 101

>sp|Q9LW49|ERF4_NICSY Ethylene-responsive transcription factor 4 OS=Nicotiana
        sylvestris GN=ERF4 PE=2 SV=1

          Length = 227

 Score =  123 bits (308), Expect = 1e-027
 Identities = 65/97 (67%), Positives = 72/97 (74%), Gaps = 7/97 (7%)
 Frame = -1

Query: 717 LKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYD 538
           LK   V TNG    +KE  YRGVRKRPWGRYAAEIRDP KK+RVWLGTFDT E AA+AYD
Sbjct:  12 LKGGNVKTNG----VKEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYD 67

Query: 537 RAALRFRGAKAKTNFPYTYVVDNNESPTQSSTVESSS 427
            AA  FRG KAKTNFP   +   N+S + SST+ESSS
Sbjct:  68 TAAREFRGPKAKTNFP---LPSENQSTSHSSTMESSS 101

>sp|Q9ZWA2|ERF77_ARATH Ethylene-responsive transcription factor 10
        OS=Arabidopsis thaliana GN=ERF10 PE=2 SV=1

          Length = 245

 Score =  119 bits (298), Expect = 2e-026
 Identities = 61/106 (57%), Positives = 75/106 (70%), Gaps = 1/106 (0%)
 Frame = -1

Query: 711 TAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRA 532
           T  +  N G E+ KE RYRGVR+RPWGRYAAEIRDP KK RVWLG+F+T E AARAYD A
Sbjct:  36 TKALAVNDGGEKSKEVRYRGVRRRPWGRYAAEIRDPVKKKRVWLGSFNTGEEAARAYDSA 95

Query: 531 ALRFRGAKAKTNFPYT-YVVDNNESPTQSSTVESSSIPDLNLGSVG 397
           A+RFRG+KA TNFP   Y   ++ +P  ++  E+ S  + NL  VG
Sbjct:  96 AIRFRGSKATTNFPLIGYYGISSATPVNNNLSETVSDGNANLPLVG 141

>sp|Q9MAI5|ERF79_ARATH Ethylene-responsive transcription factor 8 OS=Arabidopsis
        thaliana GN=ERF8 PE=2 SV=1

          Length = 185

 Score =  116 bits (290), Expect = 2e-025
 Identities = 61/81 (75%), Positives = 62/81 (76%), Gaps = 4/81 (4%)
 Frame = -1

Query: 672 KETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNF 493
           KE RYRGVRKRPWGRYAAEIRDP KKTRVWLGTFDT + AARAYD AA  FRG KAKTNF
Sbjct:  27 KEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAAARDFRGVKAKTNF 86

Query: 492 PYTYVVDNNESPTQSSTVESS 430
                V    SPTQSSTV  S
Sbjct:  87 G----VIVGSSPTQSSTVVDS 103

>sp|Q94ID6|ERF81_ARATH Ethylene-responsive transcription factor 12
        OS=Arabidopsis thaliana GN=ERF12 PE=2 SV=1

          Length = 189

 Score =  113 bits (281), Expect = 2e-024
 Identities = 54/82 (65%), Positives = 61/82 (74%)
 Frame = -1

Query: 672 KETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNF 493
           +E  YRGVRKRPWGRYAAEIRDP+KKTRVWLGTFDTPE AA AYD AA   RG KAKTNF
Sbjct:   8 REVHYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTPEEAALAYDGAARFLRGIKAKTNF 67

Query: 492 PYTYVVDNNESPTQSSTVESSS 427
           P    +D N  P+  S   +++
Sbjct:  68 PSPLSLDLNHLPSAPSAATAAA 89

>sp|Q9SXS8|ERF3_TOBAC Ethylene-responsive transcription factor 3 OS=Nicotiana
        tabacum GN=ERF3 PE=2 SV=1

          Length = 225

 Score =  112 bits (280), Expect = 2e-024
 Identities = 60/109 (55%), Positives = 69/109 (63%), Gaps = 7/109 (6%)
 Frame = -1

Query: 726 SPTLKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAAR 547
           +P   T   N +GG    KE R+RGVRKRPWGR+AAEIRDP+KKTRVWLGTFD+ E AAR
Sbjct:   9 APAPVTGEPNGSGGS---KEIRFRGVRKRPWGRFAAEIRDPWKKTRVWLGTFDSAEDAAR 65

Query: 546 AYDRAALRFRGAKAKTNFPYTYV----VDNNESPTQSSTVESSSIPDLN 412
           AYD AA   RG KAKTNFP  Y      +   +P     V+S   P  N
Sbjct:  66 AYDAAARALRGPKAKTNFPLPYAHHHQFNQGHNPNNDPFVDSRFYPQDN 114

>sp|Q9FE67|ERF80_ARATH Ethylene-responsive transcription factor 9 OS=Arabidopsis
        thaliana GN=ERF9 PE=2 SV=1

          Length = 200

 Score =  110 bits (273), Expect = 2e-023
 Identities = 54/81 (66%), Positives = 61/81 (75%), Gaps = 6/81 (7%)
 Frame = -1

Query: 714 KTAVVNTNGGDERI------KETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAA 553
           ++  V+  GG + +      KE  +RGVRKRPWGRYAAEIRDP KKTRVWLGTFDT E A
Sbjct:   9 RSIAVSEGGGGKTMTMTTMRKEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEA 68

Query: 552 ARAYDRAALRFRGAKAKTNFP 490
           ARAYD AA  FRG+KAKTNFP
Sbjct:  69 ARAYDTAAREFRGSKAKTNFP 89

>sp|O80339|ERF82_ARATH Ethylene-responsive transcription factor 3 OS=Arabidopsis
        thaliana GN=ERF3 PE=1 SV=1

          Length = 225

 Score =  108 bits (269), Expect = 5e-023
 Identities = 54/85 (63%), Positives = 62/85 (72%), Gaps = 7/85 (8%)
 Frame = -1

Query: 711 TAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRA 532
           T V   NG    +KE R+RGVRKRPWGR+AAEIRDP+KK RVWLGTFD+ E AARAYD A
Sbjct:  14 TVVAAINGS---VKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTFDSAEEAARAYDSA 70

Query: 531 ALRFRGAKAKTNFPYTYVVDNNESP 457
           A   RG KAKTNFP    +D++  P
Sbjct:  71 ARNLRGPKAKTNFP----IDSSSPP 91

>sp|Q70II3|EF110_ARATH Ethylene-responsive transcription factor ERF110
        OS=Arabidopsis thaliana GN=ERF110 PE=2 SV=2

          Length = 220

 Score =  107 bits (266), Expect = 1e-022
 Identities = 49/65 (75%), Positives = 56/65 (86%)
 Frame = -1

Query: 684 DERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKA 505
           + R K+ RYRGVR+RPWG++AAEIRDP++  RVWLGTFDT EAAARAYD AALRFRG KA
Sbjct:  69 EAREKKRRYRGVRQRPWGKWAAEIRDPHRAARVWLGTFDTAEAAARAYDEAALRFRGNKA 128

Query: 504 KTNFP 490
           K NFP
Sbjct: 129 KLNFP 133

>sp|Q9FH54|EF114_ARATH Ethylene-responsive transcription factor ERF114
        OS=Arabidopsis thaliana GN=ERF114 PE=2 SV=1

          Length = 248

 Score =  107 bits (265), Expect = 1e-022
 Identities = 59/126 (46%), Positives = 78/126 (61%), Gaps = 9/126 (7%)
 Frame = -1

Query: 768 HSNLS----NLLSLSIYMSPTLKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPY 601
           H N+S    N  S+     P  + A  + + GD  ++   YRGVR+RPWG++AAEIRDP 
Sbjct:  52 HDNISSIDDNYPSVYNPQDPNQQVAPTHQDQGD--LRRRHYRGVRQRPWGKWAAEIRDPK 109

Query: 600 KKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNFPYTYVVDNNE---SPTQSSTVESS 430
           K  RVWLGTF+T E+AA AYD AAL+F+G+KAK NFP    + +N    S  Q   +E  
Sbjct: 110 KAARVWLGTFETAESAALAYDEAALKFKGSKAKLNFPERVQLGSNSTYYSSNQIPQMEPQ 169

Query: 429 SIPDLN 412
           SIP+ N
Sbjct: 170 SIPNYN 175

>sp|Q7G1L2|RAP26_ARATH Ethylene-responsive transcription factor RAP2-6
        OS=Arabidopsis thaliana GN=RAP2-6 PE=2 SV=2

          Length = 192

 Score =  106 bits (263), Expect = 2e-022
 Identities = 53/85 (62%), Positives = 63/85 (74%), Gaps = 3/85 (3%)
 Frame = -1

Query: 663 RYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNFPY- 487
           +YRGVR+RPWG++AAEIRDP+K TRVWLGTF+T EAAARAYD AALRFRG+KAK NFP  
Sbjct:  60 KYRGVRQRPWGKWAAEIRDPHKATRVWLGTFETAEAAARAYDAAALRFRGSKAKLNFPEN 119

Query: 486 --TYVVDNNESPTQSSTVESSSIPD 418
             T  +  N    Q+S   S +  D
Sbjct: 120 VGTQTIQRNSHFLQNSMQPSLTYID 144

>sp|Q9LDE4|ERF83_ARATH Ethylene-responsive transcription factor 7 OS=Arabidopsis
        thaliana GN=ERF7 PE=1 SV=1

          Length = 244

 Score =  105 bits (260), Expect = 5e-022
 Identities = 53/79 (67%), Positives = 59/79 (74%), Gaps = 2/79 (2%)
 Frame = -1

Query: 726 SPTLKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAAR 547
           S  +  A+  T GG   +KE RYRGVRKRPWGR+AAEIRDP KK+RVWLGTFD+   AAR
Sbjct:   7 SSVVGPALPVTAGGS--VKEPRYRGVRKRPWGRFAAEIRDPLKKSRVWLGTFDSAVDAAR 64

Query: 546 AYDRAALRFRGAKAKTNFP 490
           AYD AA   RG KAKTNFP
Sbjct:  65 AYDTAARNLRGPKAKTNFP 83

>sp|Q9FGF8|ABR1_ARATH Ethylene-responsive transcription factor ABR1
        OS=Arabidopsis thaliana GN=ABR1 PE=2 SV=1

          Length = 391

 Score =  104 bits (258), Expect = 9e-022
 Identities = 51/81 (62%), Positives = 57/81 (70%)
 Frame = -1

Query: 732 YMSPTLKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAA 553
           Y   T  TA   T+         RYRGVR+RPWG++AAEIRDP+K  RVWLGTFD  E+A
Sbjct: 161 YEYTTTATASSETSSFSGDQPRRRYRGVRQRPWGKWAAEIRDPFKAARVWLGTFDNAESA 220

Query: 552 ARAYDRAALRFRGAKAKTNFP 490
           ARAYD AALRFRG KAK NFP
Sbjct: 221 ARAYDEAALRFRGNKAKLNFP 241

>sp|Q9SZ06|EF109_ARATH Ethylene-responsive transcription factor ERF109
        OS=Arabidopsis thaliana GN=ERF109 PE=2 SV=1

          Length = 268

 Score =  104 bits (258), Expect = 9e-022
 Identities = 51/111 (45%), Positives = 69/111 (62%)
 Frame = -1

Query: 726 SPTLKTAVVNTNGGDERIKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAAR 547
           SP  K A         + K+  YRGVR+RPWG++AAEIRDP + TRVWLGTF+T E AAR
Sbjct: 114 SPVAKKAEGGGKIRKRKNKKNGYRGVRQRPWGKFAAEIRDPKRATRVWLGTFETAEDAAR 173

Query: 546 AYDRAALRFRGAKAKTNFPYTYVVDNNESPTQSSTVESSSIPDLNLGSVGS 394
           AYDRAA+ FRG +AK NFP+     +  SP  +  + + +    ++ +  S
Sbjct: 174 AYDRAAIGFRGPRAKLNFPFVDYTSSVSSPVAADDIGAKASASASVSATDS 224

>sp|Q9SAD4|ESR1_ARATH Ethylene-responsive transcription factor ESR1
        OS=Arabidopsis thaliana GN=ESR1 PE=1 SV=1

          Length = 328

 Score =  102 bits (253), Expect = 3e-021
 Identities = 57/98 (58%), Positives = 61/98 (62%), Gaps = 5/98 (5%)
 Frame = -1

Query: 666 TRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNFPY 487
           TRYRGVR+RPWGRYAAEIRDP  K R WLGTFDT E AA AYD AA  FRGAKA+TNF Y
Sbjct:  55 TRYRGVRRRPWGRYAAEIRDPMSKERRWLGTFDTAEQAACAYDSAARAFRGAKARTNFTY 114

Query: 486 TYVVDNNE-----SPTQSSTVESSSIPDLNLGSVGSRF 388
              V   E     S  +SS      +P L L S    F
Sbjct: 115 PTAVIMPEPRFSFSNKKSSPSARCPLPSLPLDSSTQNF 152

>sp|Q9LY29|EF115_ARATH Ethylene-responsive transcription factor ERF115
        OS=Arabidopsis thaliana GN=ERF115 PE=2 SV=1

          Length = 263

 Score =  101 bits (249), Expect = 1e-020
 Identities = 46/74 (62%), Positives = 58/74 (78%)
 Frame = -1

Query: 675 IKETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTN 496
           +++  YRGVR+RPWG++AAEIRDP K  RVWLGTF+T EAAA AYD AAL+F+G+KAK N
Sbjct:  87 LRKRHYRGVRQRPWGKWAAEIRDPQKAARVWLGTFETAEAAALAYDNAALKFKGSKAKLN 146

Query: 495 FPYTYVVDNNESPT 454
           FP    + +N S T
Sbjct: 147 FPERAQLASNTSTT 160

>sp|Q9LYU3|EF113_ARATH Ethylene-responsive transcription factor ERF113
        OS=Arabidopsis thaliana GN=ERF113 PE=2 SV=1

          Length = 212

 Score =  99 bits (244), Expect = 4e-020
 Identities = 46/78 (58%), Positives = 58/78 (74%), Gaps = 5/78 (6%)
 Frame = -1

Query: 672 KETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNF 493
           +   YRGVR+RPWG++AAEIRDP K  RVWLGTF+T E AA AYDRAAL+F+G KAK NF
Sbjct:  35 RRRHYRGVRQRPWGKWAAEIRDPKKAARVWLGTFETAEEAALAYDRAALKFKGTKAKLNF 94

Query: 492 PYTYVVDNNESPTQSSTV 439
           P     +  + PT ++T+
Sbjct:  95 P-----ERVQGPTTTTTI 107

>sp|Q8H0T5|ERF73_ARATH Ethylene-responsive transcription factor ERF073
        OS=Arabidopsis thaliana GN=ERF073 PE=2 SV=1

          Length = 211

 Score =  97 bits (241), Expect = 8e-020
 Identities = 49/93 (52%), Positives = 59/93 (63%)
 Frame = -1

Query: 672 KETRYRGVRKRPWGRYAAEIRDPYKKTRVWLGTFDTPEAAARAYDRAALRFRGAKAKTNF 493
           + +RY+G+R+RPWGR+AAEIRDP K  RVWLGTF+T E AARAYD  A R RGAKAK NF
Sbjct:  21 QSSRYKGIRRRPWGRWAAEIRDPIKGVRVWLGTFNTAEEAARAYDLEAKRIRGAKAKLNF 80

Query: 492 PYTYVVDNNESPTQSSTVESSSIPDLNLGSVGS 394
           P                VE +   DL++  V S
Sbjct:  81 PNESSGKRKAKAKTVQQVEENHEADLDVAVVSS 113

  Database: UniProt/SwissProt
    Posted date:  Sat Aug 07 14:36:18 2010
  Number of letters in database: 182,829,261
  Number of sequences in database:  518,415

Lambda     K     H
   0.267   0.041    0.140
Gapped
Lambda     K     H
   0.267   0.041    0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 151,686,369,441
Number of Sequences: 518415
Number of Extensions: 151686369441
Number of Successful Extensions: 928182970
Number of sequences better than 0.0: 0