BLASTX 7.6.2
Query= UN39887 /QuerySize=1211
(1210 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 232 5e-060
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 226 4e-058
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 198 1e-049
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 198 1e-049
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 197 1e-049
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 196 4e-049
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 196 4e-049
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 195 1e-048
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 193 2e-048
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 191 1e-047
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 191 1e-047
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 186 3e-046
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 173 3e-042
sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=... 130 2e-029
sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1,... 122 5e-027
sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sap... 122 6e-027
sp|Q5PRC0|CH25H_DANRE Cholesterol 25-hydroxylase-like protein OS... 114 1e-024
sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS... 112 8e-024
sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS... 109 7e-023
sp|O95992|CH25H_HUMAN Cholesterol 25-hydroxylase OS=Homo sapiens... 97 2e-019
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 232 bits (590), Expect = 5e-060
Identities = 113/257 (43%), Positives = 154/257 (59%), Gaps = 4/257 (1%)
Frame = -3
Query: 1118 VESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQ-AKNNTPA 942
+E W V ++ + L G+F HE +F S +PF + FL KYKIQ K N
Sbjct: 9 IEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKKNEWK 68
Query: 941 AQEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVF 762
Q CI ++L+ H V LP+M P +A+G+ + PLPS + I F+IEDF F
Sbjct: 69 TQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYF 128
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
YW HR LH + YK +H VHH++A PFG+T+EYAHP E + LG T++GP L L TL
Sbjct: 129 YWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTL 188
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W+W+ R+ +TVE H GY FPW+ + +P +GGS FHD+HH T GNYSSTF Y+D
Sbjct: 189 WVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHE---TFVGNYSSTFTYLDK 245
Query: 401 IFGTDKGYRRLKSLKEN 351
IFGT Y K ++++
Sbjct: 246 IFGTSDKYYSRKQIRDS 262
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 226 bits (574), Expect = 4e-058
Identities = 106/244 (43%), Positives = 149/244 (61%), Gaps = 4/244 (1%)
Frame = -3
Query: 1118 VESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKI-QAKNNTPA 942
+E W V ++ + L G+F H+ +F +PF+ + F KYKI Q K N
Sbjct: 9 IEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTKENDWK 68
Query: 941 AQEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVF 762
+Q C +++L + LP+M P +A+G+ + PLPS + ++ FIIEDF F
Sbjct: 69 SQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIEDFYF 128
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
YW HR LH YK +H VHH+YA+PFG+T+EYAHP E + LG T++GP L L TL
Sbjct: 129 YWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRDLFTL 188
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W+W+ +R+ +TVE H GY FPWS +N +P +GG+ FHDYHH + GNY+STF Y+D
Sbjct: 189 WVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEVFI---GNYASTFTYLDK 245
Query: 401 IFGT 390
IFGT
Sbjct: 246 IFGT 249
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 198 bits (501), Expect = 1e-049
Identities = 99/248 (39%), Positives = 143/248 (57%), Gaps = 5/248 (2%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+++FL LP + ++ KYKIQ K T
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVF 762
Q KC LL HFC+ LPL+ +Y + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W+ +R+LET++ H GY P + N +P Y GS HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 401 IFGTDKGY 378
IFGTD Y
Sbjct: 271 IFGTDSQY 278
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 198 bits (501), Expect = 1e-049
Identities = 99/248 (39%), Positives = 143/248 (57%), Gaps = 5/248 (2%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+++FL LP + ++ KYKIQ K T
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVF 762
Q KC LL HFC+ LPL+ +Y + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W+ +R+LET++ H GY P + N +P Y GS HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 401 IFGTDKGY 378
IFGTD Y
Sbjct: 271 IFGTDSQY 278
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 197 bits (500), Expect = 1e-049
Identities = 101/263 (38%), Positives = 151/263 (57%), Gaps = 9/263 (3%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+++FL LP + F+ KYKIQ K T
Sbjct: 34 KNAWVYMLDNYTKFQIATWGSLIVHETIYFLFSLPGFLFQFIPFMRKYKIQKDKPETFEG 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVF 762
Q KC+ +L HF + LPL+ +Y + + +P W A+ L +IED
Sbjct: 94 QWKCLKGILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYFTLARCLGCAVIEDTWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W+ +R+LET++ H GY P + N++P Y G+ HD+HH GNY+STF + D
Sbjct: 214 WAWVTMRLLETIDVHSGYDIPLNPLNYIPFYTGARHHDFHHMNFI---GNYASTFTWWDR 270
Query: 401 IFGTDKGY----RRLKSLKENSD 345
IFGTD Y ++K L + S+
Sbjct: 271 IFGTDVQYHAYTEKMKKLGKKSE 293
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 196 bits (496), Expect = 4e-049
Identities = 98/248 (39%), Positives = 142/248 (57%), Gaps = 5/248 (2%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+++F LP + ++ KYKIQ K T
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFSFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVF 762
Q KC LL HFC+ LPL+ +Y + + +P W + A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I L
Sbjct: 154 YFMHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W+ +R+LET++ H GY P + N +P Y GS HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 401 IFGTDKGY 378
IFGTD Y
Sbjct: 271 IFGTDSQY 278
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 196 bits (496), Expect = 4e-049
Identities = 100/263 (38%), Positives = 150/263 (57%), Gaps = 9/263 (3%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+++FL LP + ++ KYKIQ K T
Sbjct: 34 KNAWVYMLDNYTKFQIATWGSLIVHEAIYFLFSLPGFLFQFIPYMRKYKIQKDKPETFEG 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVF 762
Q KC+ ++L HF + LPL+ +Y + + +P W A+ L +IED
Sbjct: 94 QWKCLKKILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYLTLARCLGCAVIEDTWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W+ +R+LET++ H GY P + N +P Y G+ HD+HH GNY+STF + D
Sbjct: 214 WAWVTIRLLETIDVHSGYDIPLNPLNLVPFYTGARHHDFHHMNFI---GNYASTFTWWDK 270
Query: 401 IFGTDKGY----RRLKSLKENSD 345
+FGTD Y + K L + SD
Sbjct: 271 LFGTDAQYHAYIEKSKKLGKKSD 293
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 195 bits (493), Expect = 1e-048
Identities = 97/261 (37%), Positives = 149/261 (57%), Gaps = 10/261 (3%)
Frame = -3
Query: 1124 SLVESGWQHLVTHF-SDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQAKN-N 951
+ VE W F +D + SF LHE ++F +P++ ++ + ++KIQ K
Sbjct: 28 NFVEQLWLAYYKWFDNDVVATGLMSFLLHELIYFGRCIPWMIIDAMPYFRRWKIQPKKVP 87
Query: 950 TPAAQEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 771
T A Q +C +LL HF V LP + P+ G+ +S P P ++ QI +F +ED
Sbjct: 88 TLAEQWECTRLVLLSHFTVELPQIWLFDPMCATFGLSTSVPFPPVTKMIWQITLFFFLED 147
Query: 770 FVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL----- 606
YW HR+ H Y+ +H VHH Y+ PFGL++EYAHP EI+ LG T+ P +
Sbjct: 148 TWHYWAHRLFHYGIFYRFIHKVHHRYSAPFGLSAEYAHPLEIILLGAGTVFVPLMWCYFT 207
Query: 605 TGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYS 426
HL+T+++W+ LR+ + V++H GY FPWSL+ FLP++ G+D HDYHH N+S
Sbjct: 208 HDLHLVTMYIWITLRLFQAVDSHAGYDFPWSLNKFLPIWAGADHHDYHHMAF---KDNFS 264
Query: 425 STFVYMDWIFGTDKGYRRLKS 363
S+F + D + TD+ Y + K+
Sbjct: 265 SSFRWWDAVLKTDQNYHQFKA 285
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 193 bits (490), Expect = 2e-048
Identities = 95/247 (38%), Positives = 139/247 (56%), Gaps = 5/247 (2%)
Frame = -3
Query: 1106 WQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAAQEK 930
W H++ +++ FQ+A GS +HE ++FL LP + F+ KYKIQ K T Q K
Sbjct: 37 WNHMLQNYTKFQIATWGSLIVHELIYFLFCLPGFIFQFLPFMQKYKIQPDKPETWEKQWK 96
Query: 929 CITRLLLYHFCVNLPLMMASYPVFRAMGMRSSF-PLPSWKEVSAQILFYFIIEDFVFYWG 753
C LL HFC+ LPL+ +Y + + +P W + AQ +IED Y+
Sbjct: 97 CFKMLLFNHFCIQLPLICGTYYFTEFFSIPYDWDTMPRWPFLLAQCFGCAVIEDTWHYFL 156
Query: 752 HRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLW 573
HR LH + +YK +H VHH++ +PFG+ +EYAHP E L LG +G + H+I LW W
Sbjct: 157 HRALHHRRIYKYIHKVHHDFTSPFGMQAEYAHPLETLILGAGFFIGTMVFCNHMILLWAW 216
Query: 572 MVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFG 393
+ R+LET++ H GY P + + +P Y G+ FHD+HH GNY STF + D +F
Sbjct: 217 VTFRLLETIDVHSGYDIPLNPLHLIPFYAGARFHDFHHMNFV---GNYGSTFTWWDRLFD 273
Query: 392 TDKGYRR 372
TD + +
Sbjct: 274 TDSQFNK 280
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 191 bits (483), Expect = 1e-047
Identities = 94/261 (36%), Positives = 148/261 (56%), Gaps = 5/261 (1%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+ +FL +P + ++ KYKIQ K T
Sbjct: 34 KNAWNYMLDNYTKFQIATWGSLLVHEASYFLLCVPGFIFQFIPYMQKYKIQPDKPETWEK 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSF-PLPSWKEVSAQILFYFIIEDFVF 762
Q KC L+ HF + LPL+ +Y + + +P W + AQ +IED
Sbjct: 94 QWKCFKTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWYVLLAQCFGCAVIEDAWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H VHHE+ +PFG+ +EYAHP E L LG +G + H++ L
Sbjct: 154 YFLHRLLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILGAGFFIGIVVFCNHVVLL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W++ R++ET++ H GY P + + +P Y G+ FHD+HH GNY+STF + D
Sbjct: 214 WAWVICRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHHMNFI---GNYASTFTWWDR 270
Query: 401 IFGTDKGYRRLKSLKENSDLK 339
IFGTD + K ++ L+
Sbjct: 271 IFGTDSQFIAYKEKEKKQQLR 291
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 191 bits (483), Expect = 1e-047
Identities = 94/259 (36%), Positives = 144/259 (55%), Gaps = 5/259 (1%)
Frame = -3
Query: 1115 ESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAA 939
++ W +++ +++ FQ+A GS +HE+++F LP + ++ KYKIQ K T
Sbjct: 34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFFFCLPGFLFQFIPYMKKYKIQKDKPETWEN 93
Query: 938 QEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSF-PLPSWKEVSAQILFYFIIEDFVF 762
Q KC LL HFC+ PL+ +Y + + +P W A+ +IED
Sbjct: 94 QWKCFKVLLFNHFCIQFPLICGTYYFTEYFSIPYDWETMPRWYIALARCFGCAVIEDTWH 153
Query: 761 YWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITL 582
Y+ HR+LH K +YK +H +HHE+ PFG+ +EYAHP E L LG +G L H+I L
Sbjct: 154 YFLHRLLHHKRIYKYIHKIHHEFQAPFGMEAEYAHPLETLILGTGFFIGIMLLCDHVILL 213
Query: 581 WLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDW 402
W W+ +R++ET++ H GY P + + +P Y GS HD+HH GNY+STF + D
Sbjct: 214 WAWVTVRLIETIDVHSGYDIPLNPLHLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDR 270
Query: 401 IFGTDKGYRRLKSLKENSD 345
IFGTD Y ++ ++
Sbjct: 271 IFGTDAQYNAYNEKRKKAE 289
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 186 bits (472), Expect = 3e-046
Identities = 100/279 (35%), Positives = 157/279 (56%), Gaps = 19/279 (6%)
Frame = -3
Query: 1124 SLVESGWQHLVTHFSDFQLAC-IGSFHLHESVFFLSGLPFIYLERQGFLTKYKIQAKNNT 948
+ +E W ++ ++ LA + F LHE ++F LP+ +++ + ++K+Q
Sbjct: 34 NFMEKYWAAWYSYMNNDVLATGLMFFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQ-PTKI 92
Query: 947 PAAQEK--CITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 774
P+A+E+ C+ +LL HF V + +P+ +G+ P PS K ++ +I +F++E
Sbjct: 93 PSAKEQLYCLKSVLLSHFLVEAIPIWTFHPMCEKLGITVEVPFPSLKTMALEIGLFFVLE 152
Query: 773 DFVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAL---- 606
D YW HR+ H YK +H HH YA PFGL++EYAHPAE L LGF T+ P L
Sbjct: 153 DTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMY 212
Query: 605 TGP-HLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNY 429
TG HL TL +W+ LR+ + V++H GY FPWSL+ +P + G++ HD HH GNY
Sbjct: 213 TGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDLHHHYFI---GNY 269
Query: 428 SSTFVYMDWIFGTDKG-------YRRLKSLKENSDLKQT 333
+S+F + D+ T+ G R+K EN+ K+T
Sbjct: 270 ASSFRWWDYCLDTESGPEAKASREERMKKRAENNAQKKT 308
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 173 bits (437), Expect = 3e-042
Identities = 92/239 (38%), Positives = 133/239 (55%), Gaps = 9/239 (3%)
Frame = -3
Query: 1052 FHLHESVFFLSGLPFIYLERQGFLTKYKIQ-AKNNTPAAQEKCITRLLLYHFCVNLPLMM 876
F HE +F LP+ ++R + K+KIQ K + Q +C+ +L HF V +
Sbjct: 59 FLTHEIFYFGRCLPWAIIDRIPYFRKWKIQDEKIPSDKEQWECLKSVLTSHFLVEAFPIW 118
Query: 875 ASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHHE 696
+P+ + +G+ P P ++ Q +F++ED YW HR LH YK +H HH
Sbjct: 119 FFHPLCQKIGISYQVPFPKITDMLIQWAVFFVLEDTWHYWFHRGLHYGVFYKYIHKQHHR 178
Query: 695 YATPFGLTSEYAHPAEILFLGFAT----IVGPALTGP-HLITLWLWMVLRVLETVEAHCG 531
YA PFGL +EYAHP E+ LG T IV +TG HL T+ +W++LR+ + V+AH G
Sbjct: 179 YAAPFGLAAEYAHPVEVALLGLGTVGIPIVWCLITGNLHLFTVSIWIILRLFQAVDAHSG 238
Query: 530 YHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKE 354
Y FPWSL NFLP + G+D HD HH G YSS+F + D+I T+ G + K ++
Sbjct: 239 YEFPWSLHNFLPFWAGADHHDEHHHYFI---GGYSSSFRWWDFILDTEAGPKAKKGRED 294
>sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=Macaca
fascicularis GN=QccE-20373 PE=2 SV=1
Length = 333
Score = 130 bits (326), Expect = 2e-029
Identities = 82/265 (30%), Positives = 137/265 (51%), Gaps = 23/265 (8%)
Frame = -3
Query: 1115 ESGWQHLVTHF--SDFQLACIGSFHLHESVFFLS--GLPFIY--LERQGFLTKYKIQAKN 954
++ W+ L+T F ++ L IG+ + +FF S GL + + F+++Y+IQ
Sbjct: 61 QAQWERLLTTFEGKEWILFFIGAIQV-PCLFFWSFNGLLLVVDTTGKPNFISRYRIQVGK 119
Query: 953 NTPAAQEKC--ITRLLLYHFC-VNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYF 783
N P K R +L++ C V+ P+++ YP + G LP++ ++ +
Sbjct: 120 NEPVDPVKLRQSIRTVLFNQCMVSFPMVVFLYPFLKWWGDPCRRELPTFHWFLLELAIFT 179
Query: 782 IIEDFVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAE-ILFLGFATIVGPAL 606
+IE+ +FY+ HR+LH YK +H HHE+ P G+ S YAHP E ++ IVGP +
Sbjct: 180 LIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIEHVVSNMLPAIVGPLV 239
Query: 605 TGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYS 426
G HL ++ +W L ++ T +HCGYH P FLP +FHDYHH K
Sbjct: 240 MGSHLSSITMWFSLALIITTISHCGYHLP-----FLP---SPEFHDYHH----LKFNQCY 287
Query: 425 STFVYMDWIFGTDKGYRRLKSLKEN 351
+D + GTD +++ K+ +++
Sbjct: 288 GVLGVLDHLHGTDTMFKQTKAYEKH 312
>sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1, member 2
OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1
Length = 276
Score = 122 bits (306), Expect = 5e-027
Identities = 80/264 (30%), Positives = 132/264 (50%), Gaps = 25/264 (9%)
Frame = -3
Query: 1124 SLVESGWQHLVTHFSDFQLACIGSFHLHESVFFLSGLPFIYLERQG----FLTKYKIQA- 960
S+++ W ++ + S+ + + L S +F+ LP++ + G + +YKIQ
Sbjct: 18 SVLQPIWDYVRLNHSETLRSPLFPVVLTVSSYFVLVLPYLSCDILGRKWPAIYRYKIQPD 77
Query: 959 KNNTPAAQEKCITRLLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQI----- 795
K T A C L H + +P +A + R PLP ++
Sbjct: 78 KLPTTAMLLHCSGVTLYNHILLVIPAAVAQWM------WRPPIPLPEQAPTLLELVGGVT 131
Query: 794 --LFYFIIEDFVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATI 621
L F ++ F++++ H + +WLY T H++HH Y+ PF L ++ E++ +GF T
Sbjct: 132 GNLLLFDLQYFIWHFLHHKI--RWLYVTFHAIHHNYSAPFALATQCLGGWELVTVGFWTT 189
Query: 620 VGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLP--LYGGSDFHDYHHRLLY 447
+ P L HL+T W++MV+ V +VE HCGY FPWS S +P +YGG HD HH+
Sbjct: 190 LNPVLLRCHLLTTWMFMVVHVYVSVEDHCGYDFPWSTSRLIPFGVYGGPSKHDVHHQKPN 249
Query: 446 TKSGNYSSTFVYMDWIFGTDKGYR 375
T N++ F + D +FGT +R
Sbjct: 250 T---NFAPHFSHWDKMFGTHADFR 270
>sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sapiens GN=C5orf4
PE=1 SV=1
Length = 333
Score = 122 bits (305), Expect = 6e-027
Identities = 68/219 (31%), Positives = 111/219 (50%), Gaps = 16/219 (7%)
Frame = -3
Query: 995 RQGFLTKYKIQAKNNTPAAQEKC--ITRLLLYHFC-VNLPLMMASYPVFRAMGMRSSFPL 825
+ F+++Y+IQ N P K R +L++ C ++ P+++ YP + L
Sbjct: 106 KPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKWWRDPCRREL 165
Query: 824 PSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEI 645
P++ ++ + +IE+ +FY+ HR+LH YK +H HHE+ P G+ S YAHP E
Sbjct: 166 PTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIEH 225
Query: 644 LFLG-FATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 468
IVGP + G HL ++ +W L ++ T +HCGYH P FLP +FHD
Sbjct: 226 AVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGYHLP-----FLP---SPEFHD 277
Query: 467 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKEN 351
YHH K +D + GTD +++ K+ + +
Sbjct: 278 YHH----LKFNQCYGVLGVLDHLHGTDTMFKQTKAYERH 312
>sp|Q5PRC0|CH25H_DANRE Cholesterol 25-hydroxylase-like protein OS=Danio rerio
GN=ch25h PE=2 SV=1
Length = 251
Score = 114 bits (285), Expect = 1e-024
Identities = 73/226 (32%), Positives = 110/226 (48%), Gaps = 18/226 (7%)
Frame = -3
Query: 1037 SVFFLSGLPFIYLE----RQGFLTKYKIQAKNNTPAAQEKCITRLLLYHFCVNLPLMMAS 870
+V+ LPF+ L+ + + +YKIQ K + L LY+ V + +
Sbjct: 30 TVYLSFCLPFVLLDALSPKVELIRRYKIQQKASVSWTMMWSCLALSLYNHVVYIFPLSVL 89
Query: 869 YPVFRAMGMRSSFP---LPSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVH 702
+ +R + + P W + +LF DF ++ H + H WLY+T H VH
Sbjct: 90 HWYWRPVSYLAEAPGVLRVVWDLAACLLLF-----DFQYFVWHLLHHKVPWLYRTFHKVH 144
Query: 701 HEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHF 522
H+Y + F L +EY+ E L LGF V P L G H +T L+ +L + +VE HCGY
Sbjct: 145 HKYTSTFALATEYSGAWETLSLGFFAAVNPMLLGVHPMTEMLFHMLNMWLSVEDHCGYDL 204
Query: 521 PWSLSNFLP--LYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 390
PW+ +P LYGG+ HD HH+ NY+ F + D +FGT
Sbjct: 205 PWATHRLMPFGLYGGAPHHDVHHQKF---KSNYAPYFTHWDKLFGT 247
>sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
elegans GN=F35C8.5 PE=1 SV=1
Length = 300
Score = 112 bits (278), Expect = 8e-024
Identities = 54/158 (34%), Positives = 83/158 (52%), Gaps = 4/158 (2%)
Frame = -3
Query: 824 PSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVHHEYATPFGLTSEYAHPAE 648
P+ E+ +Q+ +F+ DF ++W H I H KWLY+ HSVHH Y++PF ++++ HP E
Sbjct: 125 PTVFEMLSQLAIFFLAFDFTYFWFHYINHKVKWLYRWCHSVHHMYSSPFAASAQHLHPFE 184
Query: 647 ILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 468
+ F+G P + H +T W+W + + E H GY FP++L Y G+ HD
Sbjct: 185 LFFVGTFITTIPWIFPTHCLTYWIWFFIAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHD 244
Query: 467 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKE 354
HH T + F Y+D + G Y LK + E
Sbjct: 245 MHHLRPLT---CFQPWFNYLDRLMGYHITYADLKKMTE 279
>sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
briggsae GN=CBG14675 PE=3 SV=1
Length = 300
Score = 109 bits (270), Expect = 7e-023
Identities = 53/158 (33%), Positives = 81/158 (51%), Gaps = 4/158 (2%)
Frame = -3
Query: 824 PSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVHHEYATPFGLTSEYAHPAE 648
P+ E+ +Q+ +F+ DF ++W H H KWLY+ HSVHH Y++PF ++++ HP E
Sbjct: 125 PTVFEMVSQLAIFFLAFDFTYFWFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHLHPFE 184
Query: 647 ILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 468
+ F+ P + H +T WLW + + E H GY FP++L Y G+ HD
Sbjct: 185 LFFVATFITTVPWIFPTHCLTYWLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHD 244
Query: 467 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKE 354
HH T + F Y+D + G Y LK + E
Sbjct: 245 MHHLRPLT---CFQPWFNYLDRLMGYHITYEDLKKMTE 279
>sp|O95992|CH25H_HUMAN Cholesterol 25-hydroxylase OS=Homo sapiens GN=CH25H PE=1
SV=1
Length = 272
Score = 97 bits (240), Expect = 2e-019
Identities = 67/218 (30%), Positives = 106/218 (48%), Gaps = 16/218 (7%)
Frame = -3
Query: 1016 LPFIYLE----RQGFLTKYKIQAKNNTPAAQE--KCITRLLLYHFCVNLPLMMASYPVFR 855
LPF+ L+ L +YKI + +P+AQ+ C+ + L H P+ + +
Sbjct: 53 LPFVVLDILCSWVPALRRYKIH-PDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSP 111
Query: 854 AMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVHHEYATPFG 678
A+ + P + ILF ++ D F+ H + H WLY+T H VHH+ ++ F
Sbjct: 112 ALLPHEA---PELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFA 168
Query: 677 LTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFL 498
L ++Y E+ LGF ++ L G H +T + V+ + +VE H GY+FPWS +
Sbjct: 169 LATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLV 228
Query: 497 PL--YGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 390
P YGG HD HH + N++ F + D I GT
Sbjct: 229 PFGWYGGVVHHDLHHSHF---NCNFAPYFTHWDKILGT 263
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 155,740,548,379
Number of Sequences: 518415
Number of Extensions: 155740548379
Number of Successful Extensions: 952083542
Number of sequences better than 0.0: 0
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