BLASTX 7.6.2
Query= UN39960 /QuerySize=1132
(1131 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O94298|YOOF_SCHPO Uncharacterized hydroxylase C887.15c OS=Sch... 197 1e-049
sp|P38992|SUR2_YEAST Sphingolipid C4-hydroxylase SUR2 OS=Sacchar... 174 9e-043
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 92 6e-018
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 89 4e-017
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 89 4e-017
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 88 9e-017
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 87 1e-016
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 86 4e-016
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 86 4e-016
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 76 5e-013
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 75 6e-013
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 74 1e-012
>sp|O94298|YOOF_SCHPO Uncharacterized hydroxylase C887.15c
OS=Schizosaccharomyces pombe GN=SPBC887.15c PE=1 SV=1
Length = 293
Score = 197 bits (500), Expect = 1e-049
Identities = 83/143 (58%), Positives = 107/143 (74%)
Frame = +3
Query: 498 ARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDT 677
A Q+ A +ID+WQYF HRY+H NK LY IH+ HHRL VPY+ GALYNHP EGL+LDT
Sbjct: 131 AFQYFFAFFIIDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDT 190
Query: 678 VGGALSFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIFFTNNSAYHDVHHQLY 857
G +++L +G+SP+ A+ FF+ +T+KTVDDHCG P +P +FF NN+ YHD+HHQ Y
Sbjct: 191 FGAGVAYLAAGLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPY 250
Query: 858 VTKYNFSQPFFVMWDRILGTYLP 926
+ NFSQPFF WD +LGTY+P
Sbjct: 251 GFQKNFSQPFFTFWDHVLGTYMP 273
>sp|P38992|SUR2_YEAST Sphingolipid C4-hydroxylase SUR2 OS=Saccharomyces
cerevisiae GN=SUR2 PE=1 SV=1
Length = 349
Score = 174 bits (441), Expect = 9e-043
Identities = 80/157 (50%), Positives = 104/157 (66%), Gaps = 4/157 (2%)
Frame = +3
Query: 459 RCVYDAAI----LPSTLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPY 626
R + DAAI + A + L +DTWQYF+HR MH+NK LYK HS HH L VPY
Sbjct: 141 RIIPDAAIYYGYMYGMSALKIFAGFLFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPY 200
Query: 627 SYGALYNHPLEGLLLDTVGGALSFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFH 806
+YGAL+N+P+EG LLDT+G ++ + ++ R I F+FAT+KTVDDHCG LP +PF
Sbjct: 201 AYGALFNNPVEGFLLDTLGTGIAMTLTHLTHREQIILFTFATMKTVDDHCGYALPLDPFQ 260
Query: 807 IFFTNNSAYHDVHHQLYVTKYNFSQPFFVMWDRILGT 917
F NN+ YHD+HHQ + K NF+QPFF WD + T
Sbjct: 261 WLFPNNAVYHDIHHQQFGIKTNFAQPFFTFWDNLFQT 297
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 92 bits (227), Expect = 6e-018
Identities = 60/162 (37%), Positives = 84/162 (51%), Gaps = 16/162 (9%)
Frame = +3
Query: 468 YDAAILPS---TLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGA 638
YD +P LAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A
Sbjct: 127 YDWETMPRWYIALARCFGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKIHHEFQAPFGMEA 184
Query: 639 LYNHPLEGLLLDTVGGALSFLFSGMSPRTAIFFFSFAT---IKTVDDHCGLWLPGNPFHI 809
Y HPLE L+L G F+ + I +++ T I+T+D H G +P NP H+
Sbjct: 185 EYAHPLETLIL----GTGFFIGIMLLCDHVILLWAWVTVRLIETIDVHSGYDIPLNPLHL 240
Query: 810 F-FTNNSAYHDVHHQLYVTKYNFSQPFFVMWDRILGTYLPYS 932
F S +HD HH ++ Y + F WDRI GT Y+
Sbjct: 241 IPFYAGSRHHDFHHMNFIGNYAST---FTWWDRIFGTDAQYN 279
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 89 bits (220), Expect = 4e-017
Identities = 58/160 (36%), Positives = 82/160 (51%), Gaps = 13/160 (8%)
Frame = +3
Query: 495 LARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLD 674
LAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE L+L
Sbjct: 139 LARCFGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLIL- 195
Query: 675 TVGGALSFLFSGMSPRTAIFFFSFATIK---TVDDHCGLWLPGNPFHIF-FTNNSAYHDV 842
G F+ + I +++ TI+ T+D H G +P NP ++ F S +HD
Sbjct: 196 ---GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDF 252
Query: 843 HHQLYVTKYNFSQPFFVMWDRILGTYLPYSLDKRATGGFE 962
HH ++ Y + F WDRI GT Y+ FE
Sbjct: 253 HHMNFIGNYAST---FTWWDRIFGTDSQYNAYNEKRKKFE 289
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 89 bits (220), Expect = 4e-017
Identities = 58/160 (36%), Positives = 82/160 (51%), Gaps = 13/160 (8%)
Frame = +3
Query: 495 LARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLD 674
LAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE L+L
Sbjct: 139 LARCFGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLIL- 195
Query: 675 TVGGALSFLFSGMSPRTAIFFFSFATIK---TVDDHCGLWLPGNPFHIF-FTNNSAYHDV 842
G F+ + I +++ TI+ T+D H G +P NP ++ F S +HD
Sbjct: 196 ---GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDF 252
Query: 843 HHQLYVTKYNFSQPFFVMWDRILGTYLPYSLDKRATGGFE 962
HH ++ Y + F WDRI GT Y+ FE
Sbjct: 253 HHMNFIGNYAST---FTWWDRIFGTDSQYNAYNEKRKKFE 289
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 88 bits (217), Expect = 9e-017
Identities = 56/149 (37%), Positives = 79/149 (53%), Gaps = 13/149 (8%)
Frame = +3
Query: 495 LARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLD 674
LAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE L+L
Sbjct: 139 LARCFGCA-VIEDTWHYFMHRLLH-HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLIL- 195
Query: 675 TVGGALSFLFSGMSPRTAIFFFSFATIK---TVDDHCGLWLPGNPFHIF-FTNNSAYHDV 842
G F+ + I +++ TI+ T+D H G +P NP ++ F S +HD
Sbjct: 196 ---GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDF 252
Query: 843 HHQLYVTKYNFSQPFFVMWDRILGTYLPY 929
HH ++ Y + F WDRI GT Y
Sbjct: 253 HHMNFIGNYAST---FTWWDRIFGTDSQY 278
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 87 bits (215), Expect = 1e-016
Identities = 55/150 (36%), Positives = 80/150 (53%), Gaps = 13/150 (8%)
Frame = +3
Query: 492 TLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLL 671
TLAR A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE L+L
Sbjct: 138 TLARCLGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLIL 195
Query: 672 DTVGGALSFLFSGMSPRTAIFFFSFAT---IKTVDDHCGLWLPGNPF-HIFFTNNSAYHD 839
G F+ + I +++ T ++T+D H G +P NP +I F + +HD
Sbjct: 196 ----GTGFFIGIVLLCDHVILLWAWVTMRLLETIDVHSGYDIPLNPLNYIPFYTGARHHD 251
Query: 840 VHHQLYVTKYNFSQPFFVMWDRILGTYLPY 929
HH ++ Y + F WDRI GT + Y
Sbjct: 252 FHHMNFIGNYAST---FTWWDRIFGTDVQY 278
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 86 bits (211), Expect = 4e-016
Identities = 47/136 (34%), Positives = 73/136 (53%), Gaps = 12/136 (8%)
Frame = +3
Query: 522 LVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTVGGALSFL 701
++ D W YF+HR +H +K +YK+IH HH + P+ A Y HPLE L+L GA F+
Sbjct: 147 VIEDAWHYFLHRLLH-HKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLIL----GAGFFI 201
Query: 702 FSGMSPRTAIFFFSFA---TIKTVDDHCGLWLPGNPFHIF-FTNNSAYHDVHHQLYVTKY 869
+ + +++ ++T+D H G +P NP H+ F + +HD HH ++ Y
Sbjct: 202 GIVVFCNHVVLLWAWVICRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHHMNFIGNY 261
Query: 870 NFSQPFFVMWDRILGT 917
+ F WDRI GT
Sbjct: 262 AST---FTWWDRIFGT 274
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 86 bits (211), Expect = 4e-016
Identities = 53/150 (35%), Positives = 79/150 (52%), Gaps = 13/150 (8%)
Frame = +3
Query: 492 TLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLL 671
TLAR A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE L+L
Sbjct: 138 TLARCLGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLIL 195
Query: 672 DTVGGALSFLFSGMSPRTAIFFFSFATIK---TVDDHCGLWLPGNPFHIF-FTNNSAYHD 839
G F+ + I +++ TI+ T+D H G +P NP ++ F + +HD
Sbjct: 196 ----GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLVPFYTGARHHD 251
Query: 840 VHHQLYVTKYNFSQPFFVMWDRILGTYLPY 929
HH ++ Y + F WD++ GT Y
Sbjct: 252 FHHMNFIGNYAST---FTWWDKLFGTDAQY 278
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 76 bits (185), Expect = 5e-013
Identities = 54/150 (36%), Positives = 73/150 (48%), Gaps = 8/150 (5%)
Frame = +3
Query: 477 AILPSTLARQFIIA--MLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNH 650
A LPS F IA L+ D + Y++HR +H + F YKHIH HH P+ A Y H
Sbjct: 105 APLPSIPYLIFTIACCFLIEDFYFYWVHRALH-HGFWYKHIHKVHHDHAAPFGMTAEYAH 163
Query: 651 PLEGLLLDTVGGALSFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIF-FTNNS 827
PLE ++L FLFS T + +TV+ H G P NP + F S
Sbjct: 164 PLETVILGVGTVIGPFLFS-RDLFTLWVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGS 222
Query: 828 AYHDVHHQLYVTKYNFSQPFFVMWDRILGT 917
+HD HH+ +V Y+ + F D+I GT
Sbjct: 223 HFHDFHHETFVGNYSST---FTYLDKIFGT 249
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 75 bits (184), Expect = 6e-013
Identities = 49/147 (33%), Positives = 72/147 (48%), Gaps = 9/147 (6%)
Frame = +3
Query: 492 TLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLL 671
T+A + + ++ DTW Y+ HR H F YK+IH QHHR P+ A Y HP E L L
Sbjct: 140 TMALEIGLFFVLEDTWHYWAHRLFHYGVF-YKYIHKQHHRYAAPFGLSAEYAHPAETLSL 198
Query: 672 --DTVGGALSF-LFSG-MSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIF-FTNNSAYH 836
TVG + + +++G + T + + + VD H G P + I F + +H
Sbjct: 199 GFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHH 258
Query: 837 DVHHQLYVTKYNFSQPFFVMWDRILGT 917
D+HH ++ Y S F WD L T
Sbjct: 259 DLHHHYFIGNYASS---FRWWDYCLDT 282
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 74 bits (181), Expect = 1e-012
Identities = 49/144 (34%), Positives = 69/144 (47%), Gaps = 11/144 (7%)
Frame = +3
Query: 504 QFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLL--LDT 677
Q+ + ++ DTW Y+ HR +H F YK+IH QHHR P+ A Y HP+E L L T
Sbjct: 144 QWAVFFVLEDTWHYWFHRGLHYGVF-YKYIHKQHHRYAAPFGLAAEYAHPVEVALLGLGT 202
Query: 678 VGGAL--SFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIF--FTNNSAYHDVH 845
VG + + + T + + VD H G P + H F F + +HD H
Sbjct: 203 VGIPIVWCLITGNLHLFTVSIWIILRLFQAVDAHSGYEFPWS-LHNFLPFWAGADHHDEH 261
Query: 846 HQLYVTKYNFSQPFFVMWDRILGT 917
H ++ Y+ S F WD IL T
Sbjct: 262 HHYFIGGYSSS---FRWWDFILDT 282
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 155,740,548,379
Number of Sequences: 518415
Number of Extensions: 155740548379
Number of Successful Extensions: 952083542
Number of sequences better than 0.0: 0
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