Library    |     Search    |     Batch query    |     SNP    |     SSR  

TrEMBL blast output of UN40359


BLASTX 7.6.2

Query= UN40359 /QuerySize=1075
        (1074 letters)

Database: UniProt/TrEMBL;
          11,397,958 sequences; 3,661,877,547 total letters
                                                                  Score    E
Sequences producing significant alignments:                       (bits) Value

tr|Q8W194|Q8W194_BRAOL Zinc finger protein LSD2 OS=Brassica oler...    374   2e-101
tr|Q8W195|Q8W195_BRAOL Zinc finger protein LSD1 OS=Brassica oler...    348   9e-094
tr|P94077|P94077_ARATH Zinc-finger protein Lsd1 OS=Arabidopsis t...    307   2e-081
tr|Q8RXL2|Q8RXL2_ARATH Putative zinc-finger protein Lsd1 OS=Arab...    305   7e-081
tr|C0Z316|C0Z316_ARATH AT4G20380 protein OS=Arabidopsis thaliana...    288   1e-075
tr|C6TGF7|C6TGF7_SOYBN Putative uncharacterized protein OS=Glyci...    206   4e-051
tr|B9SV62|B9SV62_RICCO Charged multivesicular body protein 2b-B,...    201   2e-049
tr|B7FMP5|B7FMP5_MEDTR Putative uncharacterized protein OS=Medic...    200   3e-049
tr|C6T3P5|C6T3P5_SOYBN Putative uncharacterized protein OS=Glyci...    198   2e-048
tr|C6T505|C6T505_SOYBN Putative uncharacterized protein OS=Glyci...    198   2e-048
tr|B9I0U8|B9I0U8_POPTR Predicted protein OS=Populus trichocarpa ...    193   5e-047
tr|B9GIE5|B9GIE5_POPTR Predicted protein OS=Populus trichocarpa ...    183   5e-044
tr|A9SGA0|A9SGA0_PHYPA Predicted protein (Fragment) OS=Physcomit...    166   6e-039
tr|B8BMY9|B8BMY9_ORYSI Putative uncharacterized protein OS=Oryza...    124   2e-026

>tr|Q8W194|Q8W194_BRAOL Zinc finger protein LSD2 OS=Brassica oleracea GN=LSD2
        PE=2 SV=1

          Length = 193

 Score =  374 bits (958), Expect = 2e-101
 Identities = 177/198 (89%), Positives = 185/198 (93%), Gaps = 6/198 (3%)
 Frame = +3

Query: 213 MQDQLVCHGCRNTLVYPRGATNVRCALCNTINMVPLHPHPPPPPHHHHAHVGMDMAHIVC 392
           MQDQL+CHGCRNTL+YPRGATNVRCALCNTINMVPLHP PPPP   HHAH GMDMAHIVC
Sbjct:   1 MQDQLMCHGCRNTLLYPRGATNVRCALCNTINMVPLHPPPPPP---HHAHAGMDMAHIVC 57

Query: 393 GGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLMYPYGAPSVR 572
           GGCRTMLMYTRGA+SVRCSCCQTVNLVPATPP   NQPAH++CGNCRTTLMYPYGAPSVR
Sbjct:  58 GGCRTMLMYTRGASSVRCSCCQTVNLVPATPP--TNQPAHVNCGNCRTTLMYPYGAPSVR 115

Query: 573 CAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTVVVENPMSVN 752
           CAVCQFVTNVNMGNGRVPFPTN PNGT ++PG M PSTSTQSTPP QTQTVVVENPMSVN
Sbjct: 116 CAVCQFVTNVNMGNGRVPFPTNQPNGTASSPGPM-PSTSTQSTPPSQTQTVVVENPMSVN 174

Query: 753 ESGKLVSNVVVGVTTEKK 806
           ESGKLVSNVVVGVTT+KK
Sbjct: 175 ESGKLVSNVVVGVTTDKK 192

>tr|Q8W195|Q8W195_BRAOL Zinc finger protein LSD1 OS=Brassica oleracea GN=LSD1
        PE=2 SV=1

          Length = 193

 Score =  348 bits (891), Expect = 9e-094
 Identities = 168/195 (86%), Positives = 177/195 (90%), Gaps = 5/195 (2%)
 Frame = +3

Query: 213 MQDQLVCHGCRNTLVYPRGATNVRCALCNTINMVPLHPHPPPPPHHHHAHVGMDMAHIVC 392
           MQDQLVCHGCRNTL+YPRGATNVRCALC+ +NMVPLHPHPPPPP  HHAH GMDMAHIVC
Sbjct:   1 MQDQLVCHGCRNTLMYPRGATNVRCALCHIVNMVPLHPHPPPPPPPHHAHAGMDMAHIVC 60

Query: 393 GGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLMYPYGAPSVR 572
           GGCRTMLMYTRGA+SVRCSCCQTVNLVP  PP  +NQ AHI+CGNCRTTLMYPYGA SV+
Sbjct:  61 GGCRTMLMYTRGASSVRCSCCQTVNLVPGPPP--SNQVAHINCGNCRTTLMYPYGASSVK 118

Query: 573 CAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTVVVENPMSVN 752
           CAVCQFVTNVNM NGRVP  +N PNG TAAPG+M PSTST STPP QTQTVVVENPMSVN
Sbjct: 119 CAVCQFVTNVNMSNGRVPLASNRPNG-TAAPGTM-PSTST-STPPSQTQTVVVENPMSVN 175

Query: 753 ESGKLVSNVVVGVTT 797
           ESGKLVSNVVVGVTT
Sbjct: 176 ESGKLVSNVVVGVTT 190

>tr|P94077|P94077_ARATH Zinc-finger protein Lsd1 OS=Arabidopsis thaliana GN=LSD1
        PE=2 SV=1

          Length = 189

 Score =  307 bits (785), Expect = 2e-081
 Identities = 157/210 (74%), Positives = 169/210 (80%), Gaps = 30/210 (14%)
 Frame = +3

Query: 201 KPGEMQDQLVCHGCRNTLVYPRGATNVRCALCNTINMVPLHPHPPPPPHHHHAHVGMDMA 380
           K  +MQDQLVCHGCRN L+YPRGA+NVRCALCNTINMVP    PPPPPH        DMA
Sbjct:   2 KVADMQDQLVCHGCRNLLMYPRGASNVRCALCNTINMVP----PPPPPH--------DMA 49

Query: 381 HIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAH--------ISCGNCRT 536
           HI+CGGCRTMLMYTRGA+SVRCSCCQT NLV    PAH+NQ AH        I+CG+CRT
Sbjct:  50 HIICGGCRTMLMYTRGASSVRCSCCQTTNLV----PAHSNQVAHAPSSQVAQINCGHCRT 105

Query: 537 TLMYPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQT 716
           TLMYPYGA SV+CAVCQFVTNVNM NGRVP PTN PNGT     + PPSTST STPP QT
Sbjct: 106 TLMYPYGASSVKCAVCQFVTNVNMSNGRVPLPTNRPNGT-----ACPPSTST-STPPSQT 159

Query: 717 QTVVVENPMSVNESGKLVSNVVVGVTTEKK 806
           QTVVVENPMSV+ESGKLVSNVVVGVTT+KK
Sbjct: 160 QTVVVENPMSVDESGKLVSNVVVGVTTDKK 189

>tr|Q8RXL2|Q8RXL2_ARATH Putative zinc-finger protein Lsd1 OS=Arabidopsis
        thaliana GN=At4g20380 PE=2 SV=1

          Length = 184

 Score =  305 bits (780), Expect = 7e-081
 Identities = 156/206 (75%), Positives = 167/206 (81%), Gaps = 30/206 (14%)
 Frame = +3

Query: 213 MQDQLVCHGCRNTLVYPRGATNVRCALCNTINMVPLHPHPPPPPHHHHAHVGMDMAHIVC 392
           MQDQLVCHGCRN L+YPRGA+NVRCALCNTINMVP    PPPPPH        DMAHI+C
Sbjct:   1 MQDQLVCHGCRNLLMYPRGASNVRCALCNTINMVP----PPPPPH--------DMAHIIC 48

Query: 393 GGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAH--------ISCGNCRTTLMY 548
           GGCRTMLMYTRGA+SVRCSCCQT NLV    PAH+NQ AH        I+CG+CRTTLMY
Sbjct:  49 GGCRTMLMYTRGASSVRCSCCQTTNLV----PAHSNQVAHAPSSQVAQINCGHCRTTLMY 104

Query: 549 PYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTVV 728
           PYGA SV+CAVCQFVTNVNM NGRVP PTN PNGT     + PPSTST STPP QTQTVV
Sbjct: 105 PYGASSVKCAVCQFVTNVNMSNGRVPLPTNRPNGT-----ACPPSTST-STPPSQTQTVV 158

Query: 729 VENPMSVNESGKLVSNVVVGVTTEKK 806
           VENPMSV+ESGKLVSNVVVGVTT+KK
Sbjct: 159 VENPMSVDESGKLVSNVVVGVTTDKK 184

>tr|C0Z316|C0Z316_ARATH AT4G20380 protein OS=Arabidopsis thaliana GN=AT4G20380
        PE=2 SV=1

          Length = 218

 Score =  288 bits (735), Expect = 1e-075
 Identities = 147/199 (73%), Positives = 158/199 (79%), Gaps = 30/199 (15%)
 Frame = +3

Query: 201 KPGEMQDQLVCHGCRNTLVYPRGATNVRCALCNTINMVPLHPHPPPPPHHHHAHVGMDMA 380
           K  +MQDQLVCHGCRN L+YPRGA+NVRCALCNTINMVP    PPPPPH        DMA
Sbjct:   2 KVADMQDQLVCHGCRNLLMYPRGASNVRCALCNTINMVP----PPPPPH--------DMA 49

Query: 381 HIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAH--------ISCGNCRT 536
           HI+CGGCRTMLMYTRGA+SVRCSCCQT NLV    PAH+NQ AH        I+CG+CRT
Sbjct:  50 HIICGGCRTMLMYTRGASSVRCSCCQTTNLV----PAHSNQVAHAPSSQVAQINCGHCRT 105

Query: 537 TLMYPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQT 716
           TLMYPYGA SV+CAVCQFVTNVNM NGRVP PTN PNGT     + PPSTST STPP QT
Sbjct: 106 TLMYPYGASSVKCAVCQFVTNVNMSNGRVPLPTNRPNGT-----ACPPSTST-STPPSQT 159

Query: 717 QTVVVENPMSVNESGKLVS 773
           QTVVVENPMSV+ESGKLVS
Sbjct: 160 QTVVVENPMSVDESGKLVS 178

>tr|C6TGF7|C6TGF7_SOYBN Putative uncharacterized protein OS=Glycine max PE=2
        SV=1

          Length = 177

 Score =  206 bits (523), Expect = 4e-051
 Identities = 101/145 (69%), Positives = 120/145 (82%), Gaps = 7/145 (4%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GM+M+ + CGGCRT+LMYTRGA SVRCSCC TVNLV   PPA +NQ AH+ CGNCRTTLM
Sbjct:  38 GMEMSQLYCGGCRTLLMYTRGATSVRCSCCHTVNLV---PPA-SNQVAHVHCGNCRTTLM 93

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           YPYGAPSV+CA+C F+TNV+  NGR+P P + PNGTT A G++P  T++ S P  Q+QTV
Sbjct:  94 YPYGAPSVKCALCHFITNVSTNNGRLPIPVHRPNGTTNA-GTLP--TTSTSMPQSQSQTV 150

Query: 726 VVENPMSVNESGKLVSNVVVGVTTE 800
           VVENPMSV+ SGKLVSNVVVGVTT+
Sbjct: 151 VVENPMSVDSSGKLVSNVVVGVTTD 175

>tr|B9SV62|B9SV62_RICCO Charged multivesicular body protein 2b-B, putative
        OS=Ricinus communis GN=RCOM_0770230 PE=4 SV=1

          Length = 405

 Score =  201 bits (509), Expect = 2e-049
 Identities = 100/142 (70%), Positives = 114/142 (80%), Gaps = 10/142 (7%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GM+MA ++CGGCRT+LM+TRGA SVRCSCC TVNL P      + Q AHI+C NCRTTL+
Sbjct: 270 GMEMAQLICGGCRTLLMHTRGATSVRCSCCHTVNLAPV-----SGQAAHINCRNCRTTLV 324

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           YPYGAPSV+CA+C +VTNV+MGN RV  PTN PNGTT     M PSTST ST   QTQTV
Sbjct: 325 YPYGAPSVKCALCHYVTNVSMGNVRVALPTNRPNGTT----GMTPSTST-STSQSQTQTV 379

Query: 726 VVENPMSVNESGKLVSNVVVGV 791
           VVENPMSV+ESGKLVSNVVVG+
Sbjct: 380 VVENPMSVDESGKLVSNVVVGI 401

>tr|B7FMP5|B7FMP5_MEDTR Putative uncharacterized protein OS=Medicago truncatula
        PE=2 SV=1

          Length = 176

 Score =  200 bits (507), Expect = 3e-049
 Identities = 98/145 (67%), Positives = 114/145 (78%), Gaps = 8/145 (5%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GMDM+ + CGGCRT+LMYTRGA SVRCSCC TVNL P      +NQ AH+ CGN RTTLM
Sbjct:  38 GMDMSQLYCGGCRTLLMYTRGATSVRCSCCHTVNLAPV-----SNQVAHVPCGNYRTTLM 92

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           YPYGAPSV+C VC ++TN+NM NGR+P P + P+GTT     M PSTST S P  Q+QTV
Sbjct:  93 YPYGAPSVKCVVCHYITNINMSNGRLPIPGHRPHGTT--DSGMLPSTST-SMPQSQSQTV 149

Query: 726 VVENPMSVNESGKLVSNVVVGVTTE 800
           VVENPMSV+ SGKLVS+VVVGVTT+
Sbjct: 150 VVENPMSVDSSGKLVSDVVVGVTTD 174

>tr|C6T3P5|C6T3P5_SOYBN Putative uncharacterized protein OS=Glycine max PE=2
        SV=1

          Length = 179

 Score =  198 bits (501), Expect = 2e-048
 Identities = 99/143 (69%), Positives = 116/143 (81%), Gaps = 9/143 (6%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GM+M+ + CGGCRT+LMYTRGA SVRCSCC TVNLV   PPA +NQ AH+ CGNCRTTLM
Sbjct:  38 GMEMSQLYCGGCRTLLMYTRGATSVRCSCCHTVNLV---PPA-SNQVAHVHCGNCRTTLM 93

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           YPYGAPSV+CA+C F+TN N  NGR+P P + PNGTT A G++P  T++ S P  Q+QTV
Sbjct:  94 YPYGAPSVKCALCHFITNTN--NGRLPIPVHRPNGTTNA-GTLP--TTSTSMPQSQSQTV 148

Query: 726 VVENPMSVNESGKLVSNVVVGVT 794
           VVENPMSV+ SGKLV NVVVGVT
Sbjct: 149 VVENPMSVDSSGKLVGNVVVGVT 171

>tr|C6T505|C6T505_SOYBN Putative uncharacterized protein OS=Glycine max PE=2
        SV=1

          Length = 175

 Score =  198 bits (501), Expect = 2e-048
 Identities = 102/143 (71%), Positives = 117/143 (81%), Gaps = 9/143 (6%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GM+M+ + CGGCRT+LMYTRGA SVRCSCC TVNLV   PPA +NQ AH+ CGNCRTTLM
Sbjct:  38 GMEMSQLYCGGCRTLLMYTRGATSVRCSCCHTVNLV---PPA-SNQVAHVHCGNCRTTLM 93

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           YPYGAPSV+CA+C F+TN N  NGR+P P + PNGT  A G++ PSTST S P  Q+QTV
Sbjct:  94 YPYGAPSVKCALCHFITNTN--NGRLPIPVHRPNGTNNA-GTL-PSTST-SMPQSQSQTV 148

Query: 726 VVENPMSVNESGKLVSNVVVGVT 794
           VVENPMSV+ SGKLVSNVVVGVT
Sbjct: 149 VVENPMSVDSSGKLVSNVVVGVT 171

>tr|B9I0U8|B9I0U8_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_890563 PE=4 SV=1

          Length = 179

 Score =  193 bits (488), Expect = 5e-047
 Identities = 97/150 (64%), Positives = 111/150 (74%), Gaps = 11/150 (7%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GMD+A ++C GCRT+LMY RGA +VRCSCC  VN  P  P    NQ AH++CGNC+TTLM
Sbjct:  38 GMDLAQLICRGCRTLLMYARGATTVRCSCCHIVNFAPVGP----NQVAHVNCGNCQTTLM 93

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQST---PPFQT 716
           YP GAPSV+CAVC +VTN++  N RVP P N P+G     G   PSTST S      F T
Sbjct:  94 YPNGAPSVKCAVCHYVTNISTANVRVPLPANRPSGI----GGTMPSTSTVSNWTYFSFLT 149

Query: 717 QTVVVENPMSVNESGKLVSNVVVGVTTEKK 806
           QTVVVENPMSV+ESGKLVSNVVVGVTTEKK
Sbjct: 150 QTVVVENPMSVDESGKLVSNVVVGVTTEKK 179

>tr|B9GIE5|B9GIE5_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_815833 PE=4 SV=1

          Length = 174

 Score =  183 bits (462), Expect = 5e-044
 Identities = 90/143 (62%), Positives = 104/143 (72%), Gaps = 10/143 (6%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GMDMA ++C GCR++LMY  GA +VRCSCC  VN+ P       NQ AH++CGNCRT LM
Sbjct:  38 GMDMAQLICRGCRSLLMYPHGATTVRCSCCHVVNIAPG-----YNQAAHVNCGNCRTALM 92

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           YP G+PSV+C VC +VTNV+M N R+P P N PNG     G   PSTS    P  QTQTV
Sbjct:  93 YPNGSPSVKCPVCHYVTNVSMANMRIPLPANRPNGI----GGTAPSTS-MPLPHSQTQTV 147

Query: 726 VVENPMSVNESGKLVSNVVVGVT 794
           VVENPMSV+ESGKLVSNVVVGVT
Sbjct: 148 VVENPMSVDESGKLVSNVVVGVT 170

>tr|A9SGA0|A9SGA0_PHYPA Predicted protein (Fragment) OS=Physcomitrella patens
        subsp. patens GN=PHYPADRAFT_18644 PE=4 SV=1

          Length = 170

 Score =  166 bits (418), Expect = 6e-039
 Identities = 84/142 (59%), Positives = 99/142 (69%), Gaps = 11/142 (7%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           G +MA +VCGGCRT+LMY RGA SV+CSCC TVNL    P     Q AHI+CG C  TLM
Sbjct:  35 GTEMAQLVCGGCRTLLMYMRGATSVQCSCCHTVNLAMEAP-----QVAHINCGGCGMTLM 89

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGRVPFPTNLPNGTTAAPGSMPPSTSTQSTPPFQTQTV 725
           Y YGA SV+CA+CQFVT++ M N RVP P+  P    AA G  P  TS+       TQTV
Sbjct:  90 YAYGAQSVKCALCQFVTSITMLNVRVPLPSQQPRPPQAAGGPAPAPTSS------HTQTV 143

Query: 726 VVENPMSVNESGKLVSNVVVGV 791
           VVENPM+++ESGKLVSN+ VGV
Sbjct: 144 VVENPMTMDESGKLVSNMAVGV 165

>tr|B8BMY9|B8BMY9_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
        indica GN=OsI_39080 PE=4 SV=1

          Length = 172

 Score =  124 bits (311), Expect = 2e-026
 Identities = 58/102 (56%), Positives = 72/102 (70%), Gaps = 7/102 (6%)
 Frame = +3

Query: 366 GMDMAHIVCGGCRTMLMYTRGANSVRCSCCQTVNLVPATPPAHANQPAHISCGNCRTTLM 545
           GMD+AH++CGGCRT+LMYTR A SVRCSCC TVNLV        +  AH++CG C+T LM
Sbjct:  39 GMDIAHLICGGCRTLLMYTRNATSVRCSCCDTVNLV-----RPVSSIAHLNCGQCQTVLM 93

Query: 546 YPYGAPSVRCAVCQFVTNVNMGNGR--VPFPTNLPNGTTAAP 665
           YPYGAPSV+CA+C F+TN  M   R   P  T+    +T+AP
Sbjct:  94 YPYGAPSVKCAICNFITNTGMNTMRHLPPNGTSYTVPSTSAP 135

  Database: UniProt/TrEMBL
    Posted date:  Sat Aug 07 14:51:12 2010
  Number of letters in database: 3,661,877,547
  Number of sequences in database:  11,397,958

Lambda     K     H
   0.267   0.041    0.140
Gapped
Lambda     K     H
   0.267   0.041    0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,086,795,362,708
Number of Sequences: 11397958
Number of Extensions: 3086795362708
Number of Successful Extensions: 1067053429
Number of sequences better than 0.0: 0