BLASTX 7.6.2
Query= UN40562 /QuerySize=1109
(1108 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9LLC1|BCCP2_ARATH Biotin carboxyl carrier protein of acetyl-... 178 1e-043
sp|Q42783|BCCP_SOYBN Biotin carboxyl carrier protein of acetyl-C... 156 3e-037
sp|Q42533|BCCP1_ARATH Biotin carboxyl carrier protein of acetyl-... 149 3e-035
sp|P37799|BCCP_PSEAE Biotin carboxyl carrier protein of acetyl-C... 105 7e-022
sp|Q06881|BCCP_ANASP Biotin carboxyl carrier protein of acetyl-C... 100 3e-020
sp|P0ABE0|BCCP_ECO57 Biotin carboxyl carrier protein of acetyl-C... 97 2e-019
sp|P0ABD9|BCCP_ECOL6 Biotin carboxyl carrier protein of acetyl-C... 97 2e-019
sp|P0ABD8|BCCP_ECOLI Biotin carboxyl carrier protein of acetyl-C... 97 2e-019
sp|P0ABE1|BCCP_SHIFL Biotin carboxyl carrier protein of acetyl-C... 97 2e-019
sp|P43874|BCCP_HAEIN Biotin carboxyl carrier protein of acetyl-C... 87 2e-016
sp|P51283|BCCP_PORPU Biotin carboxyl carrier protein of acetyl-C... 86 4e-016
sp|Q9Z901|BCCP_CHLPN Biotin carboxyl carrier protein of acetyl-C... 85 7e-016
sp|Q1XDK5|BCCP_PORYE Biotin carboxyl carrier protein of acetyl-C... 85 7e-016
sp|O84125|BCCP_CHLTR Biotin carboxyl carrier protein of acetyl-C... 81 1e-014
sp|P49786|BCCP_BACSU Biotin carboxyl carrier protein of acetyl-C... 80 2e-014
sp|Q9PKR5|BCCP_CHLMU Biotin carboxyl carrier protein of acetyl-C... 79 5e-014
sp|O19918|BCCP_CYACA Biotin carboxyl carrier protein of acetyl-C... 72 6e-012
sp|Q9ZAA7|GCDC_ACIFV Glutaconyl-CoA decarboxylase subunit gamma ... 70 2e-011
>sp|Q9LLC1|BCCP2_ARATH Biotin carboxyl carrier protein of acetyl-CoA carboxylase
2, chloroplastic OS=Arabidopsis thaliana GN=BCCP2 PE=1 SV=1
Length = 255
Score = 178 bits (449), Expect = 1e-043
Identities = 92/119 (77%), Positives = 98/119 (82%), Gaps = 6/119 (5%)
Frame = -3
Query: 599 SPPPPMAGLPVPSSPSVS---PPTPSSPPATA---TAPSFFHPPLKSPMAGTFYRSPGPG 438
S PP MA +P + V+ PTP+S PATA +APS HPPLKSPMAGTFYRSPGPG
Sbjct: 137 SMPPVMADFSMPPAQPVALPPSPTPTSTPATAKPTSAPSSSHPPLKSPMAGTFYRSPGPG 196
Query: 437 EPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTIVP 261
EPPFVKVGDKVQKGQ+VCIIEAMKLMNEIEAEKSGTI ELLAEDGKPVSVDTPLF I P
Sbjct: 197 EPPFVKVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDGKPVSVDTPLFVIAP 255
Score = 165 bits (416), Expect = 7e-040
Identities = 101/142 (71%), Positives = 111/142 (78%), Gaps = 10/142 (7%)
Frame = -2
Query: 1017 MPSLAVLTYAKISAPNGRAGSIPGI--RRQPPQTNWISF--HVSHNQSTTWRLRATTNEV 850
M SL+V KI A N R GS+PGI +R PQ N ISF VS N S WRLRATTNEV
Sbjct: 1 MASLSV-PCVKICALNRRVGSLPGISTQRWQPQPNGISFPSDVSQNHSAFWRLRATTNEV 59
Query: 849 VSNSTTPVTNGGGLNGNVKTNVPPEPATDLSDFISKVSGLLKLVDSRDIVELELKQLDCE 670
VSNS TP+TNGG +NG KTNV PEPA +LS+F++KVSGLLKLVDS+DIVELELKQLDCE
Sbjct: 60 VSNS-TPMTNGGYMNGKAKTNV-PEPA-ELSEFMAKVSGLLKLVDSKDIVELELKQLDCE 116
Query: 669 IVIRKKEALQQ--QPAPVYHSM 610
IVIRKKEALQQ PAPVYHSM
Sbjct: 117 IVIRKKEALQQAVPPAPVYHSM 138
>sp|Q42783|BCCP_SOYBN Biotin carboxyl carrier protein of acetyl-CoA carboxylase,
chloroplastic OS=Glycine max GN=ACCB-1 PE=1 SV=1
Length = 262
Score = 156 bits (393), Expect = 3e-037
Identities = 83/121 (68%), Positives = 92/121 (76%), Gaps = 11/121 (9%)
Frame = -3
Query: 599 SPPPPMAGLPVPSSPSVSP--------PTPSSPPATATAPSFFHPPLKSPMAGTFYRSPG 444
SPPPP LP P P+ +P PTP+S PA +A S PPLKSPMAGTFYRSP
Sbjct: 145 SPPPP--ALPPPPVPASTPAPTLARATPTPTSAPAVKSAKSSL-PPLKSPMAGTFYRSPA 201
Query: 443 PGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTIV 264
PGEP FVKVGDKV+KGQVVCIIEAMKLMNEIEA++SGTI E++AED K VSVDTPLF I
Sbjct: 202 PGEPSFVKVGDKVKKGQVVCIIEAMKLMNEIEADQSGTIVEIVAEDAKSVSVDTPLFVIQ 261
Query: 263 P 261
P
Sbjct: 262 P 262
>sp|Q42533|BCCP1_ARATH Biotin carboxyl carrier protein of acetyl-CoA carboxylase
1, chloroplastic OS=Arabidopsis thaliana GN=BCCP1 PE=1 SV=2
Length = 280
Score = 149 bits (376), Expect = 3e-035
Identities = 70/113 (61%), Positives = 89/113 (78%), Gaps = 7/113 (6%)
Frame = -3
Query: 596 PPPPMAGLPVPSSP-SVSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVK 420
P P A P PS+P S+ PP+P +P ++ P +KSPMAGTFYRSP PGEPPF+K
Sbjct: 174 PAAPAAAAPAPSTPASLPPPSPPTPAKSSL------PTVKSPMAGTFYRSPAPGEPPFIK 227
Query: 419 VGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTIVP 261
VGDKVQKGQV+CI+EAMKLMNEIE++ +GT+ +++AEDGKPVS+DTPLF + P
Sbjct: 228 VGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPLFVVQP 280
Score = 74 bits (179), Expect = 2e-012
Identities = 38/60 (63%), Positives = 50/60 (83%), Gaps = 2/60 (3%)
Frame = -2
Query: 780 PEPATD--LSDFISKVSGLLKLVDSRDIVELELKQLDCEIVIRKKEALQQQPAPVYHSMI 607
PE AT+ +S+F+++V+ L+KLVDSRDIVEL+LKQLDCE+VIRKKEAL Q AP + M+
Sbjct: 100 PELATEESISEFLTQVTTLVKLVDSRDIVELQLKQLDCELVIRKKEALPQPQAPASYVMM 159
>sp|P37799|BCCP_PSEAE Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Pseudomonas aeruginosa GN=accB PE=3 SV=1
Length = 156
Score = 105 bits (261), Expect = 7e-022
Identities = 55/115 (47%), Positives = 72/115 (62%), Gaps = 6/115 (5%)
Frame = -3
Query: 590 PPMAGLPVPSSPSVSPPTPSSPPATATAPSFFHPP------LKSPMAGTFYRSPGPGEPP 429
P A P ++P +P ++ PA A A S P ++SPM GTFYR+ P
Sbjct: 42 PVYAQAPAFAAPVAAPAPAAAAPAAAAAESAPAAPKLNGNVVRSPMVGTFYRAASPTSAN 101
Query: 428 FVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTIV 264
FV+VG V+KG ++CI+EAMK+MN IEAE SGTI +L E+G+PV D PLFTIV
Sbjct: 102 FVEVGQSVKKGDILCIVEAMKMMNHIEAEVSGTIESILVENGQPVEFDQPLFTIV 156
>sp|Q06881|BCCP_ANASP Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Anabaena sp. (strain PCC 7120) GN=accB PE=1 SV=2
Length = 182
Score = 100 bits (247), Expect = 3e-020
Identities = 45/73 (61%), Positives = 58/73 (79%)
Frame = -3
Query: 479 SPMAGTFYRSPGPGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGK 300
SPM GTFYR+P PGE FV+VGD++++GQ VCIIEAMKLMNEIEA+ SG + E+L ++G+
Sbjct: 109 SPMVGTFYRAPAPGEAVFVEVGDRIRQGQTVCIIEAMKLMNEIEADVSGQVIEILVQNGE 168
Query: 299 PVSVDTPLFTIVP 261
PV + PL I P
Sbjct: 169 PVEYNQPLMRIKP 181
>sp|P0ABE0|BCCP_ECO57 Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Escherichia coli O157:H7 GN=accB PE=3 SV=1
Length = 156
Score = 97 bits (239), Expect = 2e-019
Identities = 51/107 (47%), Positives = 67/107 (62%), Gaps = 2/107 (1%)
Frame = -3
Query: 587 PMAGLPVPSSPSVSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVKVGDK 408
PM P S+ + +P T S A A A H ++SPM GTFYR+P P F++VG K
Sbjct: 51 PMMQQPAQSN-AAAPATVPSMEAPAAAEISGH-IVRSPMVGTFYRTPSPDAKAFIEVGQK 108
Query: 407 VQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
V G +CI+EAMK+MN+IEA+KSGT+ +L E G+PV D PL I
Sbjct: 109 VNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVI 155
>sp|P0ABD9|BCCP_ECOL6 Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Escherichia coli O6 GN=accB PE=3 SV=1
Length = 156
Score = 97 bits (239), Expect = 2e-019
Identities = 51/107 (47%), Positives = 67/107 (62%), Gaps = 2/107 (1%)
Frame = -3
Query: 587 PMAGLPVPSSPSVSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVKVGDK 408
PM P S+ + +P T S A A A H ++SPM GTFYR+P P F++VG K
Sbjct: 51 PMMQQPAQSN-AAAPATVPSMEAPAAAEISGH-IVRSPMVGTFYRTPSPDAKAFIEVGQK 108
Query: 407 VQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
V G +CI+EAMK+MN+IEA+KSGT+ +L E G+PV D PL I
Sbjct: 109 VNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVI 155
>sp|P0ABD8|BCCP_ECOLI Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Escherichia coli (strain K12) GN=accB PE=1 SV=1
Length = 156
Score = 97 bits (239), Expect = 2e-019
Identities = 51/107 (47%), Positives = 67/107 (62%), Gaps = 2/107 (1%)
Frame = -3
Query: 587 PMAGLPVPSSPSVSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVKVGDK 408
PM P S+ + +P T S A A A H ++SPM GTFYR+P P F++VG K
Sbjct: 51 PMMQQPAQSN-AAAPATVPSMEAPAAAEISGH-IVRSPMVGTFYRTPSPDAKAFIEVGQK 108
Query: 407 VQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
V G +CI+EAMK+MN+IEA+KSGT+ +L E G+PV D PL I
Sbjct: 109 VNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVI 155
>sp|P0ABE1|BCCP_SHIFL Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Shigella flexneri GN=accB PE=3 SV=1
Length = 156
Score = 97 bits (239), Expect = 2e-019
Identities = 51/107 (47%), Positives = 67/107 (62%), Gaps = 2/107 (1%)
Frame = -3
Query: 587 PMAGLPVPSSPSVSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVKVGDK 408
PM P S+ + +P T S A A A H ++SPM GTFYR+P P F++VG K
Sbjct: 51 PMMQQPAQSN-AAAPATVPSMEAPAAAEISGH-IVRSPMVGTFYRTPSPDAKAFIEVGQK 108
Query: 407 VQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
V G +CI+EAMK+MN+IEA+KSGT+ +L E G+PV D PL I
Sbjct: 109 VNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVI 155
>sp|P43874|BCCP_HAEIN Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Haemophilus influenzae GN=accB PE=1 SV=1
Length = 155
Score = 87 bits (214), Expect = 2e-016
Identities = 49/120 (40%), Positives = 65/120 (54%), Gaps = 6/120 (5%)
Frame = -3
Query: 614 P*FPPSPPPPMAGLPVPSSPSVSPPTPSSPPATATAPS----FFHPPLKSPMAGTFYRSP 447
P P+ A V +P+ +P P A TAP+ ++SPM GTFYRSP
Sbjct: 37 PVIAPAAVQYAAAPVVAPTPAAAP--AQVPAAATTAPAASDELSGHLVRSPMVGTFYRSP 94
Query: 446 GPGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
P FV+VG V+ G +CI+EAMK+MN IEA+K+G + +L DG V D PL I
Sbjct: 95 SPEAKAFVEVGQSVKVGDALCIVEAMKMMNRIEADKAGVVKAILINDGNAVEFDEPLIVI 154
>sp|P51283|BCCP_PORPU Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Porphyra purpurea GN=accB PE=3 SV=1
Length = 157
Score = 86 bits (211), Expect = 4e-016
Identities = 53/119 (44%), Positives = 67/119 (56%), Gaps = 7/119 (5%)
Frame = -3
Query: 602 PSPPPPMAGLPVPSS---PSVSPPTPSSPP----ATATAPSFFHPPLKSPMAGTFYRSPG 444
PS P + + SS S+ T + PP + + PS + + SPM GTFY SP
Sbjct: 37 PSKKVPQEVVSLKSSHIFKSIHSETINIPPKKTESINSKPSTNYATIVSPMVGTFYHSPA 96
Query: 443 PGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
PGE FV+VGD V+ Q VCIIEAMKLMNEIEAE G I E+L ++G V L +
Sbjct: 97 PGEKIFVQVGDIVKCNQTVCIIEAMKLMNEIEAEIEGIIIEILVKNGDIVDCGQALMKV 155
>sp|Q9Z901|BCCP_CHLPN Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Chlamydia pneumoniae GN=accB PE=3 SV=1
Length = 167
Score = 85 bits (209), Expect = 7e-016
Identities = 45/106 (42%), Positives = 62/106 (58%), Gaps = 7/106 (6%)
Frame = -3
Query: 572 PVPSSPS----VSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVKVGDKV 405
P+P+ P T +S +T T+ F + SP+ GTFY SP P P FVK GD V
Sbjct: 60 PIPTDPKKDTIKETTTENSETSTTTSSGDF---ISSPLVGTFYGSPAPDSPSFVKPGDIV 116
Query: 404 QKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
+ +VCI+EAMK+MNE++A SG + E+L +G PV + LF I
Sbjct: 117 SEDTIVCIVEAMKVMNEVKAGMSGRVLEVLITNGDPVQFGSKLFRI 162
>sp|Q1XDK5|BCCP_PORYE Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Porphyra yezoensis GN=accB PE=3 SV=1
Length = 158
Score = 85 bits (209), Expect = 7e-016
Identities = 43/71 (60%), Positives = 49/71 (69%)
Frame = -3
Query: 479 SPMAGTFYRSPGPGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGK 300
SPM GTFY SP PGE FV+VGD+V+ Q VCIIEAMKLMNEIEAE G I E+L +DG
Sbjct: 86 SPMVGTFYHSPAPGEKIFVQVGDEVKFNQTVCIIEAMKLMNEIEAEIEGKIIEILVKDGD 145
Query: 299 PVSVDTPLFTI 267
V L +
Sbjct: 146 IVDCGQALMKV 156
>sp|O84125|BCCP_CHLTR Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Chlamydia trachomatis GN=accB PE=3 SV=1
Length = 164
Score = 81 bits (199), Expect = 1e-014
Identities = 38/72 (52%), Positives = 49/72 (68%)
Frame = -3
Query: 479 SPMAGTFYRSPGPGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGK 300
SP+ GTFY SP P P F+K GD V + VVCI+EAMK+MNE++A SG + E+L +G
Sbjct: 91 SPLVGTFYGSPSPEAPAFIKPGDTVSEDTVVCIVEAMKVMNEVKAGMSGRVEEILITNGD 150
Query: 299 PVSVDTPLFTIV 264
PV + LF IV
Sbjct: 151 PVQFGSKLFRIV 162
>sp|P49786|BCCP_BACSU Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Bacillus subtilis GN=accB PE=3 SV=2
Length = 159
Score = 80 bits (197), Expect = 2e-014
Identities = 45/109 (41%), Positives = 57/109 (52%), Gaps = 2/109 (1%)
Frame = -3
Query: 587 PMAGLPVPSSPSVSPPTPSSPPATATAPSFFH--PPLKSPMAGTFYRSPGPGEPPFVKVG 414
P A + + +V P + AP + SPM GTFY S P P+V G
Sbjct: 48 PAAPVQAQAPQAVQPQAQQAAAPAQEAPKQDENLHKITSPMVGTFYASSSPEAGPYVTAG 107
Query: 413 DKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTI 267
KV + VVCI+EAMKL NEIEAE G I E+L E+G+ V PLF +
Sbjct: 108 SKVNENTVVCIVEAMKLFNEIEAEVKGEIVEVLVENGQLVEYGQPLFLV 156
>sp|Q9PKR5|BCCP_CHLMU Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Chlamydia muridarum GN=accB PE=3 SV=1
Length = 163
Score = 79 bits (193), Expect = 5e-014
Identities = 41/103 (39%), Positives = 57/103 (55%)
Frame = -3
Query: 572 PVPSSPSVSPPTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPGEPPFVKVGDKVQKGQ 393
P+PS ++ P + SP+ GTFY +P P P FVK GD V +
Sbjct: 59 PIPSDQNLGNPIVKEVGEKKEDKPVEGDFIVSPLVGTFYGAPSPESPAFVKPGDIVSEDT 118
Query: 392 VVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVSVDTPLFTIV 264
VVCI+EAMK+MNE++A +G + E+L +G PV + LF IV
Sbjct: 119 VVCIVEAMKVMNEVKAGMAGRVEEVLITNGDPVQFGSKLFRIV 161
>sp|O19918|BCCP_CYACA Biotin carboxyl carrier protein of acetyl-CoA carboxylase
OS=Cyanidium caldarium GN=accB PE=3 SV=1
Length = 152
Score = 72 bits (175), Expect = 6e-012
Identities = 36/72 (50%), Positives = 47/72 (65%)
Frame = -3
Query: 479 SPMAGTFYRSPGPGEPPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGK 300
SP++G FY S PG PFV VG V KGQ +CIIEAMK MNEIE++ G I ++ A +G
Sbjct: 77 SPISGIFYSSSKPGASPFVAVGSVVSKGQTLCIIEAMKTMNEIESDSIGKIHQICARNGD 136
Query: 299 PVSVDTPLFTIV 264
V+ + L I+
Sbjct: 137 FVTKNQVLMKII 148
>sp|Q9ZAA7|GCDC_ACIFV Glutaconyl-CoA decarboxylase subunit gamma
OS=Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
GN=gcdC PE=1 SV=1
Length = 145
Score = 70 bits (171), Expect = 2e-011
Identities = 42/111 (37%), Positives = 62/111 (55%), Gaps = 4/111 (3%)
Frame = -3
Query: 614 P*FPPSPPPPMAGLPVPSSPSVSP-PTPSSPPATATAPSFFHPPLKSPMAGTFYRSPGPG 438
P P +P A PV ++P+ +P P P++ PA A AP+ P +P +P PG
Sbjct: 28 PAAPAAPAAAPAAAPVAAAPAAAPAPAPAAAPAAAPAPA-AKPAAAAPAGSVTVSAPMPG 86
Query: 437 E--PPFVKVGDKVQKGQVVCIIEAMKLMNEIEAEKSGTITELLAEDGKPVS 291
+ VK GDKV+ G V+ I+EAMK+ NEI A + GT++E+ G V+
Sbjct: 87 KILSVNVKPGDKVEAGDVLLILEAMKMQNEIMAPEDGTVSEVRVNAGDTVA 137
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 155,740,548,379
Number of Sequences: 518415
Number of Extensions: 155740548379
Number of Successful Extensions: 952083542
Number of sequences better than 0.0: 0
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