BLASTX 7.6.2
Query= UN41450 /QuerySize=717
(716 letters)
Database: TAIR9 protein;
33,410 sequences; 13,468,323 total letters
Score E
Sequences producing significant alignments: (bits) Value
TAIR9_protein||AT3G53460.1 | Symbols: CP29 | CP29; RNA binding /... 217 8e-057
TAIR9_protein||AT3G53460.2 | Symbols: CP29 | CP29; RNA binding /... 217 8e-057
TAIR9_protein||AT2G37220.1 | Symbols: | 29 kDa ribonucleoprotei... 178 5e-045
TAIR9_protein||AT4G24770.1 | Symbols: RBP31, ATRBP31, CP31, ATRB... 116 2e-026
TAIR9_protein||AT5G50250.1 | Symbols: | 31 kDa ribonucleoprotei... 97 1e-020
TAIR9_protein||AT2G21660.1 | Symbols: ATGRP7, CCR2, GR-RBP7, GRP... 90 1e-018
TAIR9_protein||AT2G21660.2 | Symbols: ATGRP7, CCR2 | CCR2 (COLD,... 90 1e-018
TAIR9_protein||AT3G23830.1 | Symbols: GR-RBP4, GRP4 | GRP4 (GLYC... 87 7e-018
TAIR9_protein||AT3G23830.2 | Symbols: GR-RBP4, GRP4 | GRP4 (GLYC... 87 7e-018
TAIR9_protein||AT4G13850.1 | Symbols: ATGRP2, GR-RBP2, GRP2 | GR... 87 9e-018
TAIR9_protein||AT4G13850.2 | Symbols: ATGRP2 | GR-RBP2 (GLYCINE-... 87 9e-018
TAIR9_protein||AT4G13850.3 | Symbols: ATGRP2 | GR-RBP2 (GLYCINE-... 87 9e-018
TAIR9_protein||AT4G13850.4 | Symbols: ATGRP2 | GR-RBP2 (GLYCINE-... 87 9e-018
TAIR9_protein||AT1G60000.1 | Symbols: | 29 kDa ribonucleoprotei... 80 1e-015
TAIR9_protein||AT4G39260.1 | Symbols: CCR1, ATGRP8, GR-RBP8, GRP... 80 1e-015
TAIR9_protein||AT4G39260.2 | Symbols: CCR1, ATGRP8, GRP8 | GR-RB... 80 1e-015
TAIR9_protein||AT4G39260.3 | Symbols: CCR1, ATGRP8, GRP8 | GR-RB... 80 1e-015
TAIR9_protein||AT5G61030.1 | Symbols: GR-RBP3 | GR-RBP3 (glycine... 80 1e-015
>TAIR9_protein||AT3G53460.1 | Symbols: CP29 | CP29; RNA binding / poly(U)
binding | chr3:19819738-19821423 REVERSE
Length = 343
Score = 217 bits (551), Expect = 8e-057
Identities = 115/143 (80%), Positives = 123/143 (86%), Gaps = 7/143 (4%)
Frame = -3
Query: 663 RSGGYGSER-GGYGSER-SGYGSQRSGRSGYGSERSSYGSERSSYGSGSGSGSGSSDRVY 490
R GGYGSER GGYGSER GYGSQRSG GS+RSSYGS GSGSGSGSGS +R+Y
Sbjct: 206 RGGGYGSERGGGYGSERGGGYGSQRSGGGYGGSQRSSYGS-----GSGSGSGSGSGNRLY 260
Query: 489 VGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINSLNG 310
VGNLSWGVDD ALE+LF+EQGKVVEARVIYDRD+GRS+GFGFVTL S QEV +AINSLNG
Sbjct: 261 VGNLSWGVDDMALENLFNEQGKVVEARVIYDRDSGRSKGFGFVTLSSSQEVQKAINSLNG 320
Query: 309 ADLDGRQIRVSEAEARPPRRQF* 241
ADLDGRQIRVSEAEARPPR QF*
Sbjct: 321 ADLDGRQIRVSEAEARPPRGQF* 343
Score = 80 bits (197), Expect = 8e-016
Identities = 45/102 (44%), Positives = 59/102 (57%), Gaps = 5/102 (4%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
+++VGNLS+ VD L LF G V VIYD+ TGRS GFGFVT+ + EV A
Sbjct: 100 KLFVGNLSFNVDSAQLAQLFESAGNVEMVEVIYDKVTGRSRGFGFVTMSTAAEVEAAAQQ 159
Query: 318 LNGADLDGRQIRVSEAEARPPRRQF*ATMSLHCSKRNGGVGS 193
NG + +GR +RV+ A PP+R+ S R+GG GS
Sbjct: 160 FNGYEFEGRPLRVN-AGPPPPKRE----ESFSRGPRSGGYGS 196
>TAIR9_protein||AT3G53460.2 | Symbols: CP29 | CP29; RNA binding / poly(U)
binding | chr3:19819738-19821423 REVERSE
Length = 343
Score = 217 bits (551), Expect = 8e-057
Identities = 115/143 (80%), Positives = 123/143 (86%), Gaps = 7/143 (4%)
Frame = -3
Query: 663 RSGGYGSER-GGYGSER-SGYGSQRSGRSGYGSERSSYGSERSSYGSGSGSGSGSSDRVY 490
R GGYGSER GGYGSER GYGSQRSG GS+RSSYGS GSGSGSGSGS +R+Y
Sbjct: 206 RGGGYGSERGGGYGSERGGGYGSQRSGGGYGGSQRSSYGS-----GSGSGSGSGSGNRLY 260
Query: 489 VGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINSLNG 310
VGNLSWGVDD ALE+LF+EQGKVVEARVIYDRD+GRS+GFGFVTL S QEV +AINSLNG
Sbjct: 261 VGNLSWGVDDMALENLFNEQGKVVEARVIYDRDSGRSKGFGFVTLSSSQEVQKAINSLNG 320
Query: 309 ADLDGRQIRVSEAEARPPRRQF* 241
ADLDGRQIRVSEAEARPPR QF*
Sbjct: 321 ADLDGRQIRVSEAEARPPRGQF* 343
Score = 80 bits (197), Expect = 8e-016
Identities = 45/102 (44%), Positives = 59/102 (57%), Gaps = 5/102 (4%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
+++VGNLS+ VD L LF G V VIYD+ TGRS GFGFVT+ + EV A
Sbjct: 100 KLFVGNLSFNVDSAQLAQLFESAGNVEMVEVIYDKVTGRSRGFGFVTMSTAAEVEAAAQQ 159
Query: 318 LNGADLDGRQIRVSEAEARPPRRQF*ATMSLHCSKRNGGVGS 193
NG + +GR +RV+ A PP+R+ S R+GG GS
Sbjct: 160 FNGYEFEGRPLRVN-AGPPPPKRE----ESFSRGPRSGGYGS 196
>TAIR9_protein||AT2G37220.1 | Symbols: | 29 kDa ribonucleoprotein, chloroplast,
putative / RNA-binding protein cp29, putative | chr2:15634980-15636331
REVERSE
Length = 290
Score = 178 bits (449), Expect = 5e-045
Identities = 88/109 (80%), Positives = 95/109 (87%)
Frame = -3
Query: 567 RSSYGSERSSYGSGSGSGSGSSDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDT 388
RSS+GS S YG G GSG+GS +RVYVGNLSWGVDD ALESLFSEQGKVVEARVIYDRD+
Sbjct: 182 RSSFGSSGSGYGGGGGSGAGSGNRVYVGNLSWGVDDMALESLFSEQGKVVEARVIYDRDS 241
Query: 387 GRSEGFGFVTLGSPQEVTRAINSLNGADLDGRQIRVSEAEARPPRRQF* 241
GRS+GFGFVT S QEV AI SL+GADLDGRQIRVSEAEARPPRRQ+*
Sbjct: 242 GRSKGFGFVTYDSSQEVQNAIKSLDGADLDGRQIRVSEAEARPPRRQY* 290
>TAIR9_protein||AT4G24770.1 | Symbols: RBP31, ATRBP31, CP31, ATRBP33 | RBP31
(31-KDA RNA BINDING PROTEIN); RNA binding / poly(U) binding |
chr4:12766223-12767952 REVERSE
Length = 330
Score = 116 bits (288), Expect = 2e-026
Identities = 55/86 (63%), Positives = 67/86 (77%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
RVYVGNL W VD+ LE LFSE GKVVEARV+YDR+TGRS GFGFVT+ E+ AI++
Sbjct: 245 RVYVGNLPWDVDNGRLEQLFSEHGKVVEARVVYDRETGRSRGFGFVTMSDVDELNEAISA 304
Query: 318 LNGADLDGRQIRVSEAEARPPRRQF* 241
L+G +L+GR IRV+ AE RPPRR +*
Sbjct: 305 LDGQNLEGRAIRVNVAEERPPRRGY* 330
>TAIR9_protein||AT5G50250.1 | Symbols: | 31 kDa ribonucleoprotein, chloroplast,
putative / RNA-binding protein RNP-T, putative / RNA-binding protein
1/2/3, putative / RNA-binding protein cp31, putative |
chr5:20452677-20453965 REVERSE
Length = 290
Score = 97 bits (239), Expect = 1e-020
Identities = 48/82 (58%), Positives = 60/82 (73%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
R+YVGNL W VD LE LFSE GKVV+ARV+ DR+TGRS GFGFV + + EV AI +
Sbjct: 208 RIYVGNLPWDVDSGRLERLFSEHGKVVDARVVSDRETGRSRGFGFVQMSNENEVNVAIAA 267
Query: 318 LNGADLDGRQIRVSEAEARPPR 253
L+G +L+GR I+V+ AE R R
Sbjct: 268 LDGQNLEGRAIKVNVAEERTRR 289
>TAIR9_protein||AT2G21660.1 | Symbols: ATGRP7, CCR2, GR-RBP7, GRP7 | CCR2 (COLD,
CIRCADIAN RHYTHM, AND RNA BINDING 2); RNA binding / double-stranded DNA
binding / single-stranded DNA binding | chr2:9265477-9266316 REVERSE
Length = 177
Score = 90 bits (221), Expect = 1e-018
Identities = 40/79 (50%), Positives = 57/79 (72%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
R +VG L+W DD ALE+ F++ G V+++++I DR+TGRS GFGFVT + + AI
Sbjct: 9 RCFVGGLAWATDDRALETAFAQYGDVIDSKIINDRETGRSRGFGFVTFKDEKAMKDAIEG 68
Query: 318 LNGADLDGRQIRVSEAEAR 262
+NG DLDGR I V+EA++R
Sbjct: 69 MNGQDLDGRSITVNEAQSR 87
>TAIR9_protein||AT2G21660.2 | Symbols: ATGRP7, CCR2 | CCR2 (COLD, CIRCADIAN
RHYTHM, AND RNA BINDING 2); RNA binding / double-stranded DNA binding /
single-stranded DNA binding | chr2:9265477-9266316 REVERSE
Length = 160
Score = 90 bits (221), Expect = 1e-018
Identities = 40/79 (50%), Positives = 57/79 (72%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
R +VG L+W DD ALE+ F++ G V+++++I DR+TGRS GFGFVT + + AI
Sbjct: 9 RCFVGGLAWATDDRALETAFAQYGDVIDSKIINDRETGRSRGFGFVTFKDEKAMKDAIEG 68
Query: 318 LNGADLDGRQIRVSEAEAR 262
+NG DLDGR I V+EA++R
Sbjct: 69 MNGQDLDGRSITVNEAQSR 87
>TAIR9_protein||AT3G23830.1 | Symbols: GR-RBP4, GRP4 | GRP4 (GLYCINE-RICH
RNA-BINDING PROTEIN 4); RNA binding / double-stranded DNA binding /
single-stranded DNA binding | chr3:8606762-8607677 REVERSE
Length = 137
Score = 87 bits (215), Expect = 7e-018
Identities = 50/109 (45%), Positives = 66/109 (60%), Gaps = 5/109 (4%)
Frame = -3
Query: 555 GSERSSYG---SGSGSGSGSSDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTG 385
G +SS G S GS S +++VG LSWG DD++L+ F+ G+V EA VI DR+TG
Sbjct: 14 GVSQSSNGPVTSMLGSLRYMSSKLFVGGLSWGTDDSSLKQAFTSFGEVTEATVIADRETG 73
Query: 384 RSEGFGFVTLGSPQEVTRAINSLNGADLDGRQIRVSEAEAR--PPRRQF 244
RS GFGFV+ AI ++G +L+GRQIRV+ A R PR F
Sbjct: 74 RSRGFGFVSFSCEDSANNAIKEMDGKELNGRQIRVNLATERSSAPRSSF 122
>TAIR9_protein||AT3G23830.2 | Symbols: GR-RBP4, GRP4 | GRP4 (GLYCINE-RICH
RNA-BINDING PROTEIN 4); RNA binding / double-stranded DNA binding /
single-stranded DNA binding | chr3:8606762-8607677 REVERSE
Length = 137
Score = 87 bits (215), Expect = 7e-018
Identities = 50/109 (45%), Positives = 66/109 (60%), Gaps = 5/109 (4%)
Frame = -3
Query: 555 GSERSSYG---SGSGSGSGSSDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTG 385
G +SS G S GS S +++VG LSWG DD++L+ F+ G+V EA VI DR+TG
Sbjct: 14 GVSQSSNGPVTSMLGSLRYMSSKLFVGGLSWGTDDSSLKQAFTSFGEVTEATVIADRETG 73
Query: 384 RSEGFGFVTLGSPQEVTRAINSLNGADLDGRQIRVSEAEAR--PPRRQF 244
RS GFGFV+ AI ++G +L+GRQIRV+ A R PR F
Sbjct: 74 RSRGFGFVSFSCEDSANNAIKEMDGKELNGRQIRVNLATERSSAPRSSF 122
>TAIR9_protein||AT4G13850.1 | Symbols: ATGRP2, GR-RBP2, GRP2 | GR-RBP2
(GLYCINE-RICH RNA-BINDING PROTEIN 2); ATP binding / RNA binding /
double-stranded DNA binding / single-stranded DNA binding |
chr4:8021314-8022065 FORWARD
Length = 159
Score = 87 bits (214), Expect = 9e-018
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = -3
Query: 504 SDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAI 325
S ++++G LSWG DD +L F+ G VV+A+VI DR+TGRS GFGFV T AI
Sbjct: 34 STKLFIGGLSWGTDDASLRDAFAHFGDVVDAKVIVDRETGRSRGFGFVNFNDEGAATAAI 93
Query: 324 NSLNGADLDGRQIRVSEAEARP 259
+ ++G +L+GR IRV+ A RP
Sbjct: 94 SEMDGKELNGRHIRVNPANDRP 115
>TAIR9_protein||AT4G13850.2 | Symbols: ATGRP2 | GR-RBP2 (GLYCINE-RICH
RNA-BINDING PROTEIN 2); ATP binding / RNA binding / double-stranded DNA
binding / single-stranded DNA binding | chr4:8021314-8022065 FORWARD
Length = 154
Score = 87 bits (214), Expect = 9e-018
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = -3
Query: 504 SDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAI 325
S ++++G LSWG DD +L F+ G VV+A+VI DR+TGRS GFGFV T AI
Sbjct: 34 STKLFIGGLSWGTDDASLRDAFAHFGDVVDAKVIVDRETGRSRGFGFVNFNDEGAATAAI 93
Query: 324 NSLNGADLDGRQIRVSEAEARP 259
+ ++G +L+GR IRV+ A RP
Sbjct: 94 SEMDGKELNGRHIRVNPANDRP 115
>TAIR9_protein||AT4G13850.3 | Symbols: ATGRP2 | GR-RBP2 (GLYCINE-RICH
RNA-BINDING PROTEIN 2); ATP binding / RNA binding / double-stranded DNA
binding / single-stranded DNA binding | chr4:8021314-8022065 FORWARD
Length = 145
Score = 87 bits (214), Expect = 9e-018
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = -3
Query: 504 SDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAI 325
S ++++G LSWG DD +L F+ G VV+A+VI DR+TGRS GFGFV T AI
Sbjct: 34 STKLFIGGLSWGTDDASLRDAFAHFGDVVDAKVIVDRETGRSRGFGFVNFNDEGAATAAI 93
Query: 324 NSLNGADLDGRQIRVSEAEARP 259
+ ++G +L+GR IRV+ A RP
Sbjct: 94 SEMDGKELNGRHIRVNPANDRP 115
>TAIR9_protein||AT4G13850.4 | Symbols: ATGRP2 | GR-RBP2 (GLYCINE-RICH
RNA-BINDING PROTEIN 2); ATP binding / RNA binding / double-stranded DNA
binding / single-stranded DNA binding | chr4:8021314-8022065 FORWARD
Length = 130
Score = 87 bits (214), Expect = 9e-018
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = -3
Query: 504 SDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAI 325
S ++++G LSWG DD +L F+ G VV+A+VI DR+TGRS GFGFV T AI
Sbjct: 34 STKLFIGGLSWGTDDASLRDAFAHFGDVVDAKVIVDRETGRSRGFGFVNFNDEGAATAAI 93
Query: 324 NSLNGADLDGRQIRVSEAEARP 259
+ ++G +L+GR IRV+ A RP
Sbjct: 94 SEMDGKELNGRHIRVNPANDRP 115
>TAIR9_protein||AT1G60000.1 | Symbols: | 29 kDa ribonucleoprotein, chloroplast,
putative / RNA-binding protein cp29, putative | chr1:22093678-22094540
REVERSE
Length = 259
Score = 80 bits (196), Expect = 1e-015
Identities = 39/79 (49%), Positives = 54/79 (68%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
+++VGNLSW V +L F E G VV ARV++D DTGRS G+GFV S E+ A+ S
Sbjct: 178 KLFVGNLSWTVTSESLAGAFRECGDVVGARVVFDGDTGRSRGYGFVCYSSKAEMETALES 237
Query: 318 LNGADLDGRQIRVSEAEAR 262
L+G +L+GR IRV+ A+ +
Sbjct: 238 LDGFELEGRAIRVNLAQGK 256
>TAIR9_protein||AT4G39260.1 | Symbols: CCR1, ATGRP8, GR-RBP8, GRP8 | GR-RBP8;
RNA binding / nucleic acid binding / nucleotide binding |
chr4:18274166-18274958 REVERSE
Length = 170
Score = 80 bits (196), Expect = 1e-015
Identities = 36/79 (45%), Positives = 55/79 (69%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
R +VG L+W +D L+ FS+ G V+++++I DR++GRS GFGFVT + + AI
Sbjct: 7 RCFVGGLAWATNDEDLQRTFSQFGDVIDSKIINDRESGRSRGFGFVTFKDEKAMRDAIEE 66
Query: 318 LNGADLDGRQIRVSEAEAR 262
+NG +LDGR I V+EA++R
Sbjct: 67 MNGKELDGRVITVNEAQSR 85
>TAIR9_protein||AT4G39260.2 | Symbols: CCR1, ATGRP8, GRP8 | GR-RBP8; RNA binding
/ nucleic acid binding / nucleotide binding | chr4:18274166-18274958
REVERSE
Length = 127
Score = 80 bits (196), Expect = 1e-015
Identities = 36/79 (45%), Positives = 55/79 (69%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
R +VG L+W +D L+ FS+ G V+++++I DR++GRS GFGFVT + + AI
Sbjct: 7 RCFVGGLAWATNDEDLQRTFSQFGDVIDSKIINDRESGRSRGFGFVTFKDEKAMRDAIEE 66
Query: 318 LNGADLDGRQIRVSEAEAR 262
+NG +LDGR I V+EA++R
Sbjct: 67 MNGKELDGRVITVNEAQSR 85
>TAIR9_protein||AT4G39260.3 | Symbols: CCR1, ATGRP8, GRP8 | GR-RBP8; RNA binding
/ nucleic acid binding / nucleotide binding | chr4:18274166-18274958
REVERSE
Length = 93
Score = 80 bits (196), Expect = 1e-015
Identities = 36/79 (45%), Positives = 55/79 (69%)
Frame = -3
Query: 498 RVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRAINS 319
R +VG L+W +D L+ FS+ G V+++++I DR++GRS GFGFVT + + AI
Sbjct: 7 RCFVGGLAWATNDEDLQRTFSQFGDVIDSKIINDRESGRSRGFGFVTFKDEKAMRDAIEE 66
Query: 318 LNGADLDGRQIRVSEAEAR 262
+NG +LDGR I V+EA++R
Sbjct: 67 MNGKELDGRVITVNEAQSR 85
>TAIR9_protein||AT5G61030.1 | Symbols: GR-RBP3 | GR-RBP3 (glycine-rich
RNA-binding protein 3); ATP binding / RNA binding |
chr5:24560870-24562152 FORWARD
Length = 310
Score = 80 bits (196), Expect = 1e-015
Identities = 36/82 (43%), Positives = 58/82 (70%)
Frame = -3
Query: 507 SSDRVYVGNLSWGVDDTALESLFSEQGKVVEARVIYDRDTGRSEGFGFVTLGSPQEVTRA 328
SS ++++G +++ +D+ +L F++ G+VV+ RVI DR+TGRS GFGFVT S + + A
Sbjct: 38 SSSKLFIGGMAYSMDEDSLREAFTKYGEVVDTRVILDRETGRSRGFGFVTFTSSEAASSA 97
Query: 327 INSLNGADLDGRQIRVSEAEAR 262
I +L+G DL GR ++V+ A R
Sbjct: 98 IQALDGRDLHGRVVKVNYANDR 119
Database: TAIR9 protein
Posted date: Wed Jul 08 15:16:08 2009
Number of letters in database: 13,468,323
Number of sequences in database: 33,410
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,572,546,445
Number of Sequences: 33410
Number of Extensions: 20572546445
Number of Successful Extensions: 658650332
Number of sequences better than 0.0: 0
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