BLASTX 7.6.2
Query= UN42682 /QuerySize=1220
(1219 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=... 320 1e-086
sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 ... 318 6e-086
sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN... 318 6e-086
sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=... 316 2e-085
sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Ni... 312 4e-084
sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE... 310 1e-083
sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 P... 310 2e-083
sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 ... 307 1e-082
sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 ... 306 3e-082
sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosacchar... 299 3e-080
sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoi... 283 2e-075
sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces ce... 260 1e-068
sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusio... 161 1e-038
sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1... 161 2e-038
sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=N... 160 3e-038
sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1... 159 5e-038
sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 P... 156 3e-037
sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusio... 151 9e-036
sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoi... 143 4e-033
sp|O94660|NIT2_SCHPO Probable hydrolase nit2 OS=Schizosaccharomy... 120 2e-026
>sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=nit2a PE=2
SV=1
Length = 276
Score = 320 bits (820), Expect = 1e-086
Identities = 160/277 (57%), Positives = 199/277 (71%), Gaps = 6/277 (2%)
Frame = +3
Query: 165 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 344
+ KF + L Q V+ K+ N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFKLSLVQFLVSPVKSDNLNRACKLIKEAAQKGAQIVALPECFNSPYGTTYFPEYAEK 60
Query: 345 IDAGGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 524
I GE ST +LS+V+K GI +IGGSIPE +LYNTC VFG DG L KHRKIH
Sbjct: 61 IP---GE---STELLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIH 114
Query: 525 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 704
LFDID+PGKI F ES+TL+ G++ ++ DT ++G+GICYDIRF ELA IYA +G LL
Sbjct: 115 LFDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDIRFAELAQIYANKGCQLLV 174
Query: 705 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 884
YPGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A
Sbjct: 175 YPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVVAK 234
Query: 885 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 995
EE ++ AEID L + R +P+ +QRR DLY +
Sbjct: 235 AGSEETVLSAEIDLQYLAEIREQIPIRRQRRRDLYNV 271
>sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 PE=1 SV=1
Length = 276
Score = 318 bits (814), Expect = 6e-086
Identities = 160/275 (58%), Positives = 199/275 (72%), Gaps = 6/275 (2%)
Frame = +3
Query: 165 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 344
+T F + L QL +++ K+ N++ A I EAA++GAK+V LPE +NSPY FP YAE+
Sbjct: 1 MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEK 60
Query: 345 IDAGGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 524
I GE ST LSEV+K I +IGGSIPE +LYNTC VFG DG L AK+RKIH
Sbjct: 61 IP---GE---STQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIH 114
Query: 525 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 704
LFDID+PGKITF ESKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL
Sbjct: 115 LFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLV 174
Query: 705 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 884
YPGAFN+TTGP HWELLQR+RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA
Sbjct: 175 YPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAK 234
Query: 885 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 989
EEAI+ ++ID L + R +P+ +Q+R DLY
Sbjct: 235 AGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLY 269
>sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN=nit2 PE=2
SV=1
Length = 276
Score = 318 bits (814), Expect = 6e-086
Identities = 157/277 (56%), Positives = 198/277 (71%), Gaps = 6/277 (2%)
Frame = +3
Query: 165 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 344
+ KF + L Q V+ K+ N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60
Query: 345 IDAGGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 524
I GE ST LS+V+K GI +IGGSIPE +LYNTC VFG DG L KHRKIH
Sbjct: 61 IP---GE---STERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIH 114
Query: 525 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 704
LFDID+PGKI F ES+TL+ G++ ++ +T ++G+GICYDIRF ELA +Y+ +G LL
Sbjct: 115 LFDIDVPGKIRFQESETLSPGDSFSVFETPYCKVGVGICYDIRFAELAQLYSKKGCQLLV 174
Query: 705 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 884
YPGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A
Sbjct: 175 YPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAK 234
Query: 885 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 995
EE +I A+ID L + R +P+ +QRR DLY +
Sbjct: 235 AGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 271
>sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=nit2b PE=2
SV=1
Length = 276
Score = 316 bits (809), Expect = 2e-085
Identities = 157/275 (57%), Positives = 197/275 (71%), Gaps = 6/275 (2%)
Frame = +3
Query: 165 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 344
+ KF + L Q V+ K+ N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFRLSLVQFLVSPVKSDNLNKACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60
Query: 345 IDAGGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 524
I GE ST +LS+V+K GI +IGGSIPE + YNTC VFG DG L KHRKIH
Sbjct: 61 IP---GE---STELLSQVAKECGIYLIGGSIPEEDSGKFYNTCAVFGPDGTLLVKHRKIH 114
Query: 525 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 704
LFDID+PGKI F ES+TL+ G++ ++ DT ++G+GICYD+RF ELA IYA +G LL
Sbjct: 115 LFDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDMRFAELAQIYANKGCQLLV 174
Query: 705 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 884
YPGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A
Sbjct: 175 YPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTVVSPWGEVIAK 234
Query: 885 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 989
EE +I A+ID L + R +P+ +QRR +LY
Sbjct: 235 AGFEETVISADIDLQYLAEIREQIPIRRQRRDNLY 269
>sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Nit2 PE=1 SV=1
Length = 276
Score = 312 bits (798), Expect = 4e-084
Identities = 158/270 (58%), Positives = 192/270 (71%), Gaps = 6/270 (2%)
Frame = +3
Query: 186 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGGE 365
L QL V++ K+ NI+ A + EAA +GA +V LPE +NSPY + FP YAE+I GE
Sbjct: 8 LIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPECFNSPYGTNYFPEYAEKIP---GE 64
Query: 366 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 545
ST LSEV+K I +IGGSIPE +LYNTC VFG DG L KHRKIHLFDID+P
Sbjct: 65 ---STKKLSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGPDGNLLVKHRKIHLFDIDVP 121
Query: 546 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 725
GKITF ESKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL YPGAFNM
Sbjct: 122 GKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYARRGCQLLVYPGAFNM 181
Query: 726 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 905
TTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G+VL EE I
Sbjct: 182 TTGPAHWELLQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLTKAGTEETI 241
Query: 906 IIAEIDYSILEQRRTSLPLSKQRRGDLYQL 995
+ ++ID L + R +P+ KQ+R DLY +
Sbjct: 242 LYSDIDLKKLSEIRQQIPILKQKRADLYSV 271
>sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE=2 SV=1
Length = 276
Score = 310 bits (794), Expect = 1e-083
Identities = 153/270 (56%), Positives = 192/270 (71%), Gaps = 6/270 (2%)
Frame = +3
Query: 186 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGGE 365
L QL V++ K+ N++ A I EA+ +GA++V LPE +NSPY FP YAE+I
Sbjct: 8 LIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEKI------ 61
Query: 366 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 545
ST LSEV+K + +IGGSIPE+ +LYNTC VFG DG L KHRK+HLFDID+P
Sbjct: 62 PGDSTQKLSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDIDVP 121
Query: 546 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 725
GKITF ES+TL+ G++ ++ DT R+G+GICYDIRF ELA IYA RG LL YPGAFN+
Sbjct: 122 GKITFQESETLSPGDSFSLFDTPYCRVGLGICYDIRFAELAQIYAQRGCQLLVYPGAFNL 181
Query: 726 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 905
TTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EE I
Sbjct: 182 TTGPAHWELLQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAKAGTEETI 241
Query: 906 IIAEIDYSILEQRRTSLPLSKQRRGDLYQL 995
+ A+ID L + R +P+ Q+R DLY++
Sbjct: 242 VYADIDLKKLAEIRQQIPIFSQKRSDLYEV 271
>sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 PE=2 SV=1
Length = 277
Score = 310 bits (793), Expect = 2e-083
Identities = 153/272 (56%), Positives = 195/272 (71%), Gaps = 6/272 (2%)
Frame = +3
Query: 192 QLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGGEAS 371
QL V+ K N+ A+ ++EAA +GAK+V+LPE +NSPY F YAE+I GE
Sbjct: 10 QLHVSKIKADNLGRAQTLVKEAAGQGAKVVVLPECFNSPYGTGFFKEYAEKIP---GE-- 64
Query: 372 PSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGK 551
ST +LSE +K+ GI ++GGSIPE G +LYNTC VFG DG L HRKIHLFDID+PGK
Sbjct: 65 -STQVLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVFGPDGTLLVTHRKIHLFDIDVPGK 123
Query: 552 ITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTT 731
I F ES+TL+ G++ ++ +T ++G+GICYDIRF ELA IYA +G LL YPGAFNMTT
Sbjct: 124 IRFQESETLSPGKSLSMFETPYCKVGVGICYDIRFAELAQIYAKKGCQLLVYPGAFNMTT 183
Query: 732 GPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIII 911
GP HWELLQR RA DNQ+YVAT SPARD A Y AWGHS++I P+GEV++ EE+++
Sbjct: 184 GPAHWELLQRGRAVDNQVYVATASPARDETASYVAWGHSSVINPWGEVISKAGSEESVVY 243
Query: 912 AEIDYSILEQRRTSLPLSKQRRGDLYQLVDVQ 1007
A+ID L R +P++KQRR DLY + VQ
Sbjct: 244 ADIDLQYLADVRQQIPITKQRRNDLYSVNSVQ 275
>sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 PE=1 SV=1
Length = 276
Score = 307 bits (786), Expect = 1e-082
Identities = 155/268 (57%), Positives = 190/268 (70%), Gaps = 6/268 (2%)
Frame = +3
Query: 186 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGGE 365
L QL V++ K+ N++ A + EAA +GA +V LPE +NSPY FP YAE+I GE
Sbjct: 8 LIQLQVSSIKSDNLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDYAEKIP---GE 64
Query: 366 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 545
ST LSEV+K I +IGGSIPE +LYNTC VFG DG L KHRKIHLFDID+P
Sbjct: 65 ---STQKLSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVP 121
Query: 546 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 725
GKITF ESKTL+ G++ + DT ++G+GICYD+RF ELA IYA RG LL YPGAFN+
Sbjct: 122 GKITFQESKTLSPGDSFSTFDTPYCKVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNL 181
Query: 726 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 905
TTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G+VL EE I
Sbjct: 182 TTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEETI 241
Query: 906 IIAEIDYSILEQRRTSLPLSKQRRGDLY 989
+ ++ID L + R +P+ KQ+R DLY
Sbjct: 242 LYSDIDLKKLAEIRQQIPILKQKRADLY 269
>sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 PE=3 SV=1
Length = 275
Score = 306 bits (782), Expect = 3e-082
Identities = 157/268 (58%), Positives = 192/268 (71%), Gaps = 7/268 (2%)
Frame = +3
Query: 186 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGGE 365
L QL +++ + N++ A I EAA++GAK+V LPE +NSPY FP YAE+I GE
Sbjct: 8 LIQLQISSINSDNVTRACSFIREAATQGAKIVSLPECFNSPYGTKYFPEYAEKIP---GE 64
Query: 366 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 545
ST LSEV+K I +IGGSIPE +LYNTC VFG DG L AK+RKIHLFDID+P
Sbjct: 65 ---STQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVP 121
Query: 546 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 725
GKITF ESKTL+ G++ DT R+G+GICYD+RF ELA IYA RG LL YPGAFN+
Sbjct: 122 GKITFQESKTLSPGDSFCTFDT-YCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNL 180
Query: 726 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 905
TTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EEAI
Sbjct: 181 TTGPAHWELLQRGRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAI 240
Query: 906 IIAEIDYSILEQRRTSLPLSKQRRGDLY 989
+ ++ID L + R +P+ +Q+R DLY
Sbjct: 241 VYSDIDLKKLAEIRQQIPVFRQKRSDLY 268
>sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosaccharomyces pombe
GN=SPAC26A3.11 PE=2 SV=1
Length = 322
Score = 299 bits (765), Expect = 3e-080
Identities = 149/287 (51%), Positives = 198/287 (68%), Gaps = 4/287 (1%)
Frame = +3
Query: 138 SALPLPAPPLTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSN 317
SA L F IGL QL+ T DK+ N+ A+ + EAA G+ +++LPEI+NSPY
Sbjct: 32 SASSLVPKDFRAFRIGLVQLANTKDKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPYGT 91
Query: 318 DSFPVYAEEIDAGGGEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGE 497
F YAE I+ E+SPS LS ++K + GGSIPER +LYNT VF G+
Sbjct: 92 GYFNQYAEPIE----ESSPSYQALSSMAKDTKTYLFGGSIPERKDGKLYNTAMVFDPSGK 147
Query: 498 LKAKHRKIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIY 677
L A HRKIHLFDIDIPG ++F ES +L+ G+ T+VDT+ G+ G+GICYDIRF ELAMI
Sbjct: 148 LIAVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTMVDTEYGKFGLGICYDIRFPELAMIA 207
Query: 678 AARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLI 857
A G ++ YPGAFN++TGPLHWELL RARA DN+++VA C+PARD A Y +WGHST++
Sbjct: 208 ARNGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVV 267
Query: 858 GPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLV 998
PFG+V+ATT+ + +I+ A+ID S++ R S+P+ QRR D+Y V
Sbjct: 268 DPFGKVIATTDEKPSIVYADIDPSVMSTARNSVPIYTQRRFDVYSEV 314
>sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoideum GN=nit2
PE=3 SV=1
Length = 328
Score = 283 bits (724), Expect = 2e-075
Identities = 142/281 (50%), Positives = 192/281 (68%), Gaps = 8/281 (2%)
Frame = +3
Query: 174 FNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDA 353
F QL +K N+ +A K I+EAA GAKL+ LPE +NSPYS +F Y+E D
Sbjct: 53 FKFAGIQLLCGDNKEENVQNAIKHIDEAAKNGAKLISLPECFNSPYSTSTFEKYSETED- 111
Query: 354 GGGEASPSTAMLSEVSKRLGITIIGGSIPE--RVGDRLYNTCCVFGSDGELKAKHRKIHL 527
GE + LSE +KR I ++GGSIPE + ++YNTC +F GE+ KHRKIHL
Sbjct: 112 --GE---TVKKLSEAAKRNQIFLVGGSIPEIDKATGKIYNTCFIFNDKGEVVKKHRKIHL 166
Query: 528 FDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCY 707
FDID+P KI F ES+TLT G++ ++VD +IG+ ICYDIRF ELAM+Y+ GA L Y
Sbjct: 167 FDIDVPNKIRFKESETLTPGDSFSVVDIGYCKIGVAICYDIRFPELAMLYSKMGAKFLIY 226
Query: 708 PGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATT 887
PGAFNM TGP HWELLQR RA DNQ++VA SPAR+ + Y AWGHST++ +G +LATT
Sbjct: 227 PGAFNMVTGPAHWELLQRGRAVDNQVFVAAISPARNPSSTYQAWGHSTIVNSWGTILATT 286
Query: 888 EHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLVDVQR 1010
+ ++II ++ID + L + R+S+P+ Q+R DLY+L +++
Sbjct: 287 DEHQSIIYSDIDLNTLNETRSSIPIYSQKRDDLYKLDSIKK 327
>sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces cerevisiae
GN=NIT3 PE=1 SV=1
Length = 291
Score = 260 bits (664), Expect = 1e-068
Identities = 136/282 (48%), Positives = 185/282 (65%), Gaps = 7/282 (2%)
Frame = +3
Query: 171 KFNIGLCQLSVTA-DKTRNISHAKKAIEEAASK--GAKLVLLPEIWNSPYSNDSFPVYAE 341
K + L QLS ++ DK N+ A IE A + KLV+LPE +NSPYS D F Y+E
Sbjct: 10 KIKVALVQLSGSSPDKMANLQRAATFIERAMKEQPDTKLVVLPECFNSPYSTDQFRKYSE 69
Query: 342 EIDAGGGEASPSTAMLSEVSKRLGITIIGGSIPE--RVGDRLYNTCCVFGSDGELKAKHR 515
I+ E S S LS ++ + I ++GG+IPE D++YNT +F DG+L KHR
Sbjct: 70 VINP--KEPSTSVQFLSNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLIDKHR 127
Query: 516 KIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 695
K+HLFD+DIP I+F ES+TL+ GE T +DT G+ G+GICYD+RF ELAM+ A +GA
Sbjct: 128 KVHLFDVDIPNGISFHESETLSPGEKSTTIDTKYGKFGVGICYDMRFPELAMLSARKGAF 187
Query: 696 LLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEV 875
+ YP AFN TGPLHW LL R+RA DNQ+YV CSPAR+ + Y A+GHS ++ P G++
Sbjct: 188 AMIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKI 247
Query: 876 LATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLVD 1001
+A E II AE+D ++E R ++PL+KQRR D+Y V+
Sbjct: 248 VAEAGEGEEIIYAELDPEVIESFRQAVPLTKQRRFDVYSDVN 289
>sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Drosophila melanogaster GN=NitFhit PE=1 SV=1
Length = 460
Score = 161 bits (406), Expect = 1e-038
Identities = 110/333 (33%), Positives = 168/333 (50%), Gaps = 16/333 (4%)
Frame = +3
Query: 180 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 359
I + Q+ T+DK N+S + ++ A S+ A ++ LPE + + +S + I+
Sbjct: 35 IAVGQMRSTSDKAANLSQVIELVDRAKSQNACMLFLPECCD--FVGES---RTQTIELSE 89
Query: 360 GEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDID 539
G A E++K I I G + ER +++N + GEL A +RK+H+FD+
Sbjct: 90 GLDGELMAQYRELAKCNKIWISLGGVHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVT 149
Query: 540 IPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYP 710
++ ES T+T G E P V T VG+IG+ ICYD+RF E A++ GA+LL YP
Sbjct: 150 TK-EVRLRESDTVTPGYCLERP--VSTPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYP 206
Query: 711 GAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLA-TT 887
AF TG HWE+L RARA + Q +V + +WGHS ++ P+G VLA +
Sbjct: 207 SAFTYATGKAHWEILLRARAIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCS 266
Query: 888 EHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLVDVQRLNSK*VQQRERESESEMLS 1067
E E I AE+D S+L+ ++P + RR D+Y L + + Q R +
Sbjct: 267 EQELDIGTAEVDLSVLQSLYQTMPCFEHRRNDIYALTAYNLRSKEPTQDRPFATNIVDKR 326
Query: 1068 LAFVESENSLVTCNFLTCQRCNKKKKNFLTCQR 1166
F ESE+ C T RC K ++ +R
Sbjct: 327 TIFYESEH----CFAFTNLRCVVKGHVLVSTKR 355
>sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1 PE=1 SV=2
Length = 327
Score = 161 bits (405), Expect = 2e-038
Identities = 96/278 (34%), Positives = 146/278 (52%), Gaps = 15/278 (5%)
Frame = +3
Query: 180 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 359
+ +CQ++ T DK +N + + EAA GA L LPE ++ + + ++ E GG
Sbjct: 49 VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSE-PLGG 107
Query: 360 GEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 524
T ++++ G+ + G ER D ++YN + S G + A +RK H
Sbjct: 108 KLLEEYT----QLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTH 163
Query: 525 LFDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 695
L D++IPG+ ES + G E+P V T G+IG+ +CYD+RF EL++ A GA
Sbjct: 164 LCDVEIPGQGPMCESNSTMPGPSLESP--VSTPAGKIGLAVCYDMRFPELSLALAQAGAE 221
Query: 696 LLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEV 875
+L YP AF TGP HWE+L RARA + Q YV + ++GHS ++ P+G V
Sbjct: 222 ILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTV 281
Query: 876 LATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 989
+A + +A ID + L Q R LP+ + RR DLY
Sbjct: 282 VARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLY 319
>sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=Nit1 PE=2 SV=1
Length = 292
Score = 160 bits (403), Expect = 3e-038
Identities = 99/281 (35%), Positives = 146/281 (51%), Gaps = 21/281 (7%)
Frame = +3
Query: 180 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 359
+ +CQ++ T +K N + ++EA GA L LPE ++ N + + E G
Sbjct: 14 VAVCQVTSTPNKQENFKTCAELVQEATRLGACLAFLPEAFDFIARNPAETLLLSEPLDG- 72
Query: 360 GEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 524
S++++ GI + G ER D ++YN + S G + A +RK H
Sbjct: 73 ----DLLGQYSQLARECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASYRKTH 128
Query: 525 LFDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 695
L D++IPG+ ES G E P V T G++G+ ICYD+RF EL++ A GA
Sbjct: 129 LCDVEIPGQGPMRESNYTMPGYALEPP--VKTPAGKVGLAICYDMRFPELSLKLAQAGAE 186
Query: 696 LLCYPGAFNMTTGPLHWELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPF 866
+L YP AF TGP HWE+L RARA ++Q YV A C ++ A Y GHS ++ P+
Sbjct: 187 ILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPW 243
Query: 867 GEVLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 989
G V+A+ + +A ID L+Q R LP+ + RR DLY
Sbjct: 244 GTVVASCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 284
>sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1 PE=2 SV=1
Length = 323
Score = 159 bits (401), Expect = 5e-038
Identities = 98/279 (35%), Positives = 144/279 (51%), Gaps = 17/279 (6%)
Frame = +3
Query: 180 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 359
+ +CQ++ T +K N + ++EAA GA L LPE ++ N + + E G
Sbjct: 45 VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPLNG- 103
Query: 360 GEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 524
S++++ GI + G ER D ++YN + S G + A +RK H
Sbjct: 104 ----DLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTH 159
Query: 525 LFDIDIPGKITFMESK-TLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLL 701
L D++IPG+ ES T G V T G++G+ ICYD+RF EL++ A GA +L
Sbjct: 160 LCDVEIPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGAEIL 219
Query: 702 CYPGAFNMTTGPLHWELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPFGE 872
Y AF TGP HWE+L RARA ++Q YV A C ++ A Y GHS ++ P+G
Sbjct: 220 TYSSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPWGT 276
Query: 873 VLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 989
V+A + +A ID L+Q R LP+ + RR DLY
Sbjct: 277 VVARCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 315
>sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 PE=2 SV=1
Length = 328
Score = 156 bits (394), Expect = 3e-037
Identities = 93/278 (33%), Positives = 143/278 (51%), Gaps = 15/278 (5%)
Frame = +3
Query: 180 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 359
+ +CQ++ T DK +N + I EAA GA L LPE ++ + E +G
Sbjct: 50 VAVCQVTSTPDKEQNFKTCAELIREAARLGACLAFLPEAFDFIARDPEETRRLSEPLSGN 109
Query: 360 GEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 524
+++++ G+ + G ER D ++YN + + G + A +RK H
Sbjct: 110 -----LLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVIMNNMGSVVATYRKTH 164
Query: 525 LFDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 695
L D++IPG+ ES + G E+P + T G+IG+ ICYD+RF EL++ GA
Sbjct: 165 LCDVEIPGQGPMRESNSTIPGPSLESP--ISTPAGKIGLAICYDMRFPELSLALVQAGAE 222
Query: 696 LLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEV 875
+L YP AF TGP HWE+L RARA + Q YV + ++GHS ++ P+G V
Sbjct: 223 ILTYPSAFGSVTGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTV 282
Query: 876 LATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 989
+A + +A ID + L+Q R LP+ + RR DLY
Sbjct: 283 VARCSEGPGLCLARIDLNYLQQLRKQLPVFQHRRPDLY 320
>sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Caenorhabditis elegans GN=nft-1 PE=1 SV=1
Length = 440
Score = 151 bits (381), Expect = 9e-036
Identities = 77/179 (43%), Positives = 107/179 (59%), Gaps = 1/179 (0%)
Frame = +3
Query: 462 YNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAG-ETPTIVDTDVGRIGIGI 638
+NT + SDG +A++ K+HLFD++IPGK+ MES+ AG E VDT +GR+G+ I
Sbjct: 109 WNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGLSI 168
Query: 639 CYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDS 818
CYD+RF EL++ RGA LL +P AF + TG HWE L RARA +NQ YV +
Sbjct: 169 CYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAH 228
Query: 819 GAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 995
++GHS ++ P+G V+A + AEID S ++ R P+ RR DLY L
Sbjct: 229 NPKRQSYGHSMVVDPWGAVVAQCSERVDMCFAEIDLSYVDTLREMQPVFSHRRSDLYTL 287
>sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoideum GN=nit1-1
PE=3 SV=1
Length = 291
Score = 143 bits (358), Expect = 4e-033
Identities = 90/285 (31%), Positives = 148/285 (51%), Gaps = 21/285 (7%)
Frame = +3
Query: 180 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIW-------NSPYSNDSFPVYA 338
IGL Q++ T +K N K+ IE+A L LPE + + S D+ A
Sbjct: 15 IGLGQITSTNNKEDNFRKCKEMIEKAVENKVNLFCLPECFAFISGGIHQFESRDN----A 70
Query: 339 EEIDAGGGEASPSTAMLSEVSKRLGITIIGGSIPERV----GDRLYNTCCVFGSDGELKA 506
E +D GG +++K+ I + G E++ D +YNT + S+G +
Sbjct: 71 EYLDQKGG----IIERYKDLAKQNNIWLSLGGFHEKILDDPNDMIYNTHLIIDSNGVIVC 126
Query: 507 KHRKIHLFDIDIPGK-ITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAA 683
++RK+HLFD+DIP K + ESK + G + D+ VG++G+ ICYD+RF EL +
Sbjct: 127 EYRKMHLFDVDIPSKGVKMNESKVVKGGNDLVVCDSPVGKLGLSICYDLRFPELYLSLRR 186
Query: 684 RGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGP 863
A +L P AF +TG HW+ L +ARA +NQ YV + D + +++GHS +I P
Sbjct: 187 MDAQILLVPSAFMKSTGEAHWKPLLQARAIENQTYVIAAAQTGDHHSKRSSYGHSMIIDP 246
Query: 864 FGEVL-ATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 995
+G+VL ++ I +ID + R ++P+ ++ + Y++
Sbjct: 247 WGKVLHDLPDNLNDIAFVDIDLDYISTCRENIPVFNHKKLNNYKI 291
>sp|O94660|NIT2_SCHPO Probable hydrolase nit2 OS=Schizosaccharomyces pombe
GN=nit2 PE=2 SV=1
Length = 276
Score = 120 bits (300), Expect = 2e-026
Identities = 69/192 (35%), Positives = 105/192 (54%), Gaps = 7/192 (3%)
Frame = +3
Query: 438 PERVGDRLYNTCC-VFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDT 611
P +V ++L N+ + GE+ +++ K HLFD++I T ES T GE T
Sbjct: 87 PSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKESNTTLRGEAILPPCKT 146
Query: 612 DVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYV 791
+G++G IC+DIRF E A+ GAH++ YP AF TG HWE+L RARA D+Q YV
Sbjct: 147 PLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAAHWEVLLRARALDSQCYV 206
Query: 792 ATCSPARDSGAGYTAWGHSTLIGPFGEVLA---TTEHEEAIIIAEIDYSILEQRRTSLPL 962
+ ++GHS ++ P+G V+A +I A++D ++++ RT +PL
Sbjct: 207 IAPAQGGKHNEKRASYGHSMIVDPWGTVIAQYSDISSPNGLIFADLDLNLVDHVRTYIPL 266
Query: 963 SKQRRGDLYQLV 998
RR DLY +
Sbjct: 267 --LRRNDLYPTI 276
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 164,931,892,944
Number of Sequences: 518415
Number of Extensions: 164931892944
Number of Successful Extensions: 1021520109
Number of sequences better than 0.0: 0
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