BLASTX 7.6.2
Query= UN43471 /QuerySize=1313
(1312 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=... 320 2e-086
sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 ... 318 8e-086
sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN... 318 8e-086
sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=... 316 3e-085
sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Ni... 312 6e-084
sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE... 310 1e-083
sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 P... 310 2e-083
sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 ... 307 1e-082
sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 ... 305 4e-082
sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosacchar... 299 3e-080
sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoi... 284 1e-075
sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces ce... 260 2e-068
sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=N... 161 1e-038
sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1... 161 2e-038
sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1... 161 2e-038
sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 P... 158 1e-037
sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusio... 151 1e-035
sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusio... 142 8e-033
sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoi... 140 2e-032
sp|O94660|NIT2_SCHPO Probable hydrolase nit2 OS=Schizosaccharomy... 120 3e-026
>sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=nit2a PE=2
SV=1
Length = 276
Score = 320 bits (819), Expect = 2e-086
Identities = 158/277 (57%), Positives = 197/277 (71%), Gaps = 6/277 (2%)
Frame = +2
Query: 242 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 421
+ KF + L Q V+ K+ N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFKLSLVQFLVSPVKSDNLNRACKLIKEAAQKGAQIVALPECFNSPYGTTYFPEYAEK 60
Query: 422 IDAGGVEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 601
I ST +LS+V+K GI +IGGSIPE +LYNTC VFG DG L KHRKIH
Sbjct: 61 I------PGESTELLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIH 114
Query: 602 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 781
LFDID+PGKI F ES+TL+ G++ ++ DT ++G+GICYDIRF ELA IYA +G LL
Sbjct: 115 LFDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDIRFAELAQIYANKGCQLLV 174
Query: 782 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 961
YPGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A
Sbjct: 175 YPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVVAK 234
Query: 962 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
EE ++ AEID L + R +P+ +QRR DLY +
Sbjct: 235 AGSEETVLSAEIDLQYLAEIREQIPIRRQRRRDLYNV 271
>sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 PE=1 SV=1
Length = 276
Score = 318 bits (813), Expect = 8e-086
Identities = 158/275 (57%), Positives = 197/275 (71%), Gaps = 6/275 (2%)
Frame = +2
Query: 242 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 421
+T F + L QL +++ K+ N++ A I EAA++GAK+V LPE +NSPY FP YAE+
Sbjct: 1 MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEK 60
Query: 422 IDAGGVEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 601
I ST LSEV+K I +IGGSIPE +LYNTC VFG DG L AK+RKIH
Sbjct: 61 I------PGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIH 114
Query: 602 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 781
LFDID+PGKITF ESKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL
Sbjct: 115 LFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLV 174
Query: 782 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 961
YPGAFN+TTGP HWELLQR+RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA
Sbjct: 175 YPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAK 234
Query: 962 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
EEAI+ ++ID L + R +P+ +Q+R DLY
Sbjct: 235 AGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLY 269
>sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN=nit2 PE=2
SV=1
Length = 276
Score = 318 bits (813), Expect = 8e-086
Identities = 155/277 (55%), Positives = 196/277 (70%), Gaps = 6/277 (2%)
Frame = +2
Query: 242 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 421
+ KF + L Q V+ K+ N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFRLSLVQFLVSPVKSENLNRACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60
Query: 422 IDAGGVEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 601
I ST LS+V+K GI +IGGSIPE +LYNTC VFG DG L KHRKIH
Sbjct: 61 I------PGESTERLSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIH 114
Query: 602 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 781
LFDID+PGKI F ES+TL+ G++ ++ +T ++G+GICYDIRF ELA +Y+ +G LL
Sbjct: 115 LFDIDVPGKIRFQESETLSPGDSFSVFETPYCKVGVGICYDIRFAELAQLYSKKGCQLLV 174
Query: 782 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 961
YPGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A
Sbjct: 175 YPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAK 234
Query: 962 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
EE +I A+ID L + R +P+ +QRR DLY +
Sbjct: 235 AGSEETVISADIDLEYLAEIREQIPIRRQRRHDLYSV 271
>sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=nit2b PE=2
SV=1
Length = 276
Score = 316 bits (808), Expect = 3e-085
Identities = 155/275 (56%), Positives = 195/275 (70%), Gaps = 6/275 (2%)
Frame = +2
Query: 242 LTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEE 421
+ KF + L Q V+ K+ N++ A K I+EAA KGA++V LPE +NSPY FP YAE+
Sbjct: 1 MAKFRLSLVQFLVSPVKSDNLNKACKLIKEAAQKGAQIVALPECFNSPYGTKYFPEYAEK 60
Query: 422 IDAGGVEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIH 601
I ST +LS+V+K GI +IGGSIPE + YNTC VFG DG L KHRKIH
Sbjct: 61 I------PGESTELLSQVAKECGIYLIGGSIPEEDSGKFYNTCAVFGPDGTLLVKHRKIH 114
Query: 602 LFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLC 781
LFDID+PGKI F ES+TL+ G++ ++ DT ++G+GICYD+RF ELA IYA +G LL
Sbjct: 115 LFDIDVPGKIRFQESETLSPGDSFSVFDTPYCKVGVGICYDMRFAELAQIYANKGCQLLV 174
Query: 782 YPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLAT 961
YPGAFNMTTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A
Sbjct: 175 YPGAFNMTTGPAHWELLQRARALDNQVYVATASPARDEKASYVAWGHSTVVSPWGEVIAK 234
Query: 962 TEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
EE +I A+ID L + R +P+ +QRR +LY
Sbjct: 235 AGFEETVISADIDLQYLAEIREQIPIRRQRRDNLY 269
>sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Nit2 PE=1 SV=1
Length = 276
Score = 312 bits (797), Expect = 6e-084
Identities = 156/270 (57%), Positives = 190/270 (70%), Gaps = 6/270 (2%)
Frame = +2
Query: 263 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGVE 442
L QL V++ K+ NI+ A + EAA +GA +V LPE +NSPY + FP YAE+I
Sbjct: 8 LIQLQVSSIKSDNITRACSLVREAAKQGANIVSLPECFNSPYGTNYFPEYAEKI------ 61
Query: 443 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 622
ST LSEV+K I +IGGSIPE +LYNTC VFG DG L KHRKIHLFDID+P
Sbjct: 62 PGESTKKLSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGPDGNLLVKHRKIHLFDIDVP 121
Query: 623 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 802
GKITF ESKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL YPGAFNM
Sbjct: 122 GKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYARRGCQLLVYPGAFNM 181
Query: 803 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 982
TTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G+VL EE I
Sbjct: 182 TTGPAHWELLQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLTKAGTEETI 241
Query: 983 IIAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
+ ++ID L + R +P+ KQ+R DLY +
Sbjct: 242 LYSDIDLKKLSEIRQQIPILKQKRADLYSV 271
>sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE=2 SV=1
Length = 276
Score = 310 bits (794), Expect = 1e-083
Identities = 153/270 (56%), Positives = 192/270 (71%), Gaps = 6/270 (2%)
Frame = +2
Query: 263 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGVE 442
L QL V++ K+ N++ A I EA+ +GA++V LPE +NSPY FP YAE+I
Sbjct: 8 LIQLQVSSIKSENLTRACGLIREASKQGAQIVSLPECFNSPYGTKYFPDYAEKI------ 61
Query: 443 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 622
ST LSEV+K + +IGGSIPE+ +LYNTC VFG DG L KHRK+HLFDID+P
Sbjct: 62 PGDSTQKLSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDIDVP 121
Query: 623 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 802
GKITF ES+TL+ G++ ++ DT R+G+GICYDIRF ELA IYA RG LL YPGAFN+
Sbjct: 122 GKITFQESETLSPGDSFSLFDTPYCRVGLGICYDIRFAELAQIYAQRGCQLLVYPGAFNL 181
Query: 803 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 982
TTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EE I
Sbjct: 182 TTGPAHWELLQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAKAGTEETI 241
Query: 983 IIAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
+ A+ID L + R +P+ Q+R DLY++
Sbjct: 242 VYADIDLKKLAEIRQQIPIFSQKRSDLYEV 271
>sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 PE=2 SV=1
Length = 277
Score = 310 bits (792), Expect = 2e-083
Identities = 151/272 (55%), Positives = 193/272 (70%), Gaps = 6/272 (2%)
Frame = +2
Query: 269 QLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGVEAS 448
QL V+ K N+ A+ ++EAA +GAK+V+LPE +NSPY F YAE+I
Sbjct: 10 QLHVSKIKADNLGRAQTLVKEAAGQGAKVVVLPECFNSPYGTGFFKEYAEKI------PG 63
Query: 449 PSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGK 628
ST +LSE +K+ GI ++GGSIPE G +LYNTC VFG DG L HRKIHLFDID+PGK
Sbjct: 64 ESTQVLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVFGPDGTLLVTHRKIHLFDIDVPGK 123
Query: 629 ITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTT 808
I F ES+TL+ G++ ++ +T ++G+GICYDIRF ELA IYA +G LL YPGAFNMTT
Sbjct: 124 IRFQESETLSPGKSLSMFETPYCKVGVGICYDIRFAELAQIYAKKGCQLLVYPGAFNMTT 183
Query: 809 GPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIII 988
GP HWELLQR RA DNQ+YVAT SPARD A Y AWGHS++I P+GEV++ EE+++
Sbjct: 184 GPAHWELLQRGRAVDNQVYVATASPARDETASYVAWGHSSVINPWGEVISKAGSEESVVY 243
Query: 989 AEIDYSILEQRRTSLPLSKQRRGDLYQLVDVQ 1084
A+ID L R +P++KQRR DLY + VQ
Sbjct: 244 ADIDLQYLADVRQQIPITKQRRNDLYSVNSVQ 275
>sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 PE=1 SV=1
Length = 276
Score = 307 bits (785), Expect = 1e-082
Identities = 153/268 (57%), Positives = 188/268 (70%), Gaps = 6/268 (2%)
Frame = +2
Query: 263 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGVE 442
L QL V++ K+ N++ A + EAA +GA +V LPE +NSPY FP YAE+I
Sbjct: 8 LIQLQVSSIKSDNLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDYAEKI------ 61
Query: 443 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 622
ST LSEV+K I +IGGSIPE +LYNTC VFG DG L KHRKIHLFDID+P
Sbjct: 62 PGESTQKLSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVP 121
Query: 623 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 802
GKITF ESKTL+ G++ + DT ++G+GICYD+RF ELA IYA RG LL YPGAFN+
Sbjct: 122 GKITFQESKTLSPGDSFSTFDTPYCKVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNL 181
Query: 803 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 982
TTGP HWELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G+VL EE I
Sbjct: 182 TTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEETI 241
Query: 983 IIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
+ ++ID L + R +P+ KQ+R DLY
Sbjct: 242 LYSDIDLKKLAEIRQQIPILKQKRADLY 269
>sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 PE=3 SV=1
Length = 275
Score = 305 bits (781), Expect = 4e-082
Identities = 155/268 (57%), Positives = 190/268 (70%), Gaps = 7/268 (2%)
Frame = +2
Query: 263 LCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGVE 442
L QL +++ + N++ A I EAA++GAK+V LPE +NSPY FP YAE+I
Sbjct: 8 LIQLQISSINSDNVTRACSFIREAATQGAKIVSLPECFNSPYGTKYFPEYAEKI------ 61
Query: 443 ASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIP 622
ST LSEV+K I +IGGSIPE +LYNTC VFG DG L AK+RKIHLFDID+P
Sbjct: 62 PGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVP 121
Query: 623 GKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNM 802
GKITF ESKTL+ G++ DT R+G+GICYD+RF ELA IYA RG LL YPGAFN+
Sbjct: 122 GKITFQESKTLSPGDSFCTFDT-YCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNL 180
Query: 803 TTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAI 982
TTGP HWELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EEAI
Sbjct: 181 TTGPAHWELLQRGRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAI 240
Query: 983 IIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
+ ++ID L + R +P+ +Q+R DLY
Sbjct: 241 VYSDIDLKKLAEIRQQIPVFRQKRSDLY 268
>sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosaccharomyces pombe
GN=SPAC26A3.11 PE=2 SV=1
Length = 322
Score = 299 bits (765), Expect = 3e-080
Identities = 149/287 (51%), Positives = 198/287 (68%), Gaps = 4/287 (1%)
Frame = +2
Query: 215 SALPLPAPPLTKFNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSN 394
SA L F IGL QL+ T DK+ N+ A+ + EAA G+ +++LPEI+NSPY
Sbjct: 32 SASSLVPKDFRAFRIGLVQLANTKDKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPYGT 91
Query: 395 DSFPVYAEEIDAGGVEASPSTAMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGE 574
F YAE I+ E+SPS LS ++K + GGSIPER +LYNT VF G+
Sbjct: 92 GYFNQYAEPIE----ESSPSYQALSSMAKDTKTYLFGGSIPERKDGKLYNTAMVFDPSGK 147
Query: 575 LKAKHRKIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIY 754
L A HRKIHLFDIDIPG ++F ES +L+ G+ T+VDT+ G+ G+GICYDIRF ELAMI
Sbjct: 148 LIAVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTMVDTEYGKFGLGICYDIRFPELAMIA 207
Query: 755 AARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLI 934
A G ++ YPGAFN++TGPLHWELL RARA DN+++VA C+PARD A Y +WGHST++
Sbjct: 208 ARNGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVV 267
Query: 935 GPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLV 1075
PFG+V+ATT+ + +I+ A+ID S++ R S+P+ QRR D+Y V
Sbjct: 268 DPFGKVIATTDEKPSIVYADIDPSVMSTARNSVPIYTQRRFDVYSEV 314
>sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoideum GN=nit2
PE=3 SV=1
Length = 328
Score = 284 bits (725), Expect = 1e-075
Identities = 141/281 (50%), Positives = 191/281 (67%), Gaps = 8/281 (2%)
Frame = +2
Query: 251 FNIGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDA 430
F QL +K N+ +A K I+EAA GAKL+ LPE +NSPYS +F Y+E D
Sbjct: 53 FKFAGIQLLCGDNKEENVQNAIKHIDEAAKNGAKLISLPECFNSPYSTSTFEKYSETEDG 112
Query: 431 GGVEASPSTAMLSEVSKRLGITIIGGSIPE--RVGDRLYNTCCVFGSDGELKAKHRKIHL 604
V+ LSE +KR I ++GGSIPE + ++YNTC +F GE+ KHRKIHL
Sbjct: 113 ETVK------KLSEAAKRNQIFLVGGSIPEIDKATGKIYNTCFIFNDKGEVVKKHRKIHL 166
Query: 605 FDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCY 784
FDID+P KI F ES+TLT G++ ++VD +IG+ ICYDIRF ELAM+Y+ GA L Y
Sbjct: 167 FDIDVPNKIRFKESETLTPGDSFSVVDIGYCKIGVAICYDIRFPELAMLYSKMGAKFLIY 226
Query: 785 PGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATT 964
PGAFNM TGP HWELLQR RA DNQ++VA SPAR+ + Y AWGHST++ +G +LATT
Sbjct: 227 PGAFNMVTGPAHWELLQRGRAVDNQVFVAAISPARNPSSTYQAWGHSTIVNSWGTILATT 286
Query: 965 EHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLVDVQR 1087
+ ++II ++ID + L + R+S+P+ Q+R DLY+L +++
Sbjct: 287 DEHQSIIYSDIDLNTLNETRSSIPIYSQKRDDLYKLDSIKK 327
>sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces cerevisiae
GN=NIT3 PE=1 SV=1
Length = 291
Score = 260 bits (664), Expect = 2e-068
Identities = 136/282 (48%), Positives = 185/282 (65%), Gaps = 7/282 (2%)
Frame = +2
Query: 248 KFNIGLCQLSVTA-DKTRNISHAKKAIEEAASK--GAKLVLLPEIWNSPYSNDSFPVYAE 418
K + L QLS ++ DK N+ A IE A + KLV+LPE +NSPYS D F Y+E
Sbjct: 10 KIKVALVQLSGSSPDKMANLQRAATFIERAMKEQPDTKLVVLPECFNSPYSTDQFRKYSE 69
Query: 419 EIDAGGVEASPSTAMLSEVSKRLGITIIGGSIPE--RVGDRLYNTCCVFGSDGELKAKHR 592
I+ E S S LS ++ + I ++GG+IPE D++YNT +F DG+L KHR
Sbjct: 70 VINP--KEPSTSVQFLSNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLIDKHR 127
Query: 593 KIHLFDIDIPGKITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 772
K+HLFD+DIP I+F ES+TL+ GE T +DT G+ G+GICYD+RF ELAM+ A +GA
Sbjct: 128 KVHLFDVDIPNGISFHESETLSPGEKSTTIDTKYGKFGVGICYDMRFPELAMLSARKGAF 187
Query: 773 LLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEV 952
+ YP AFN TGPLHW LL R+RA DNQ+YV CSPAR+ + Y A+GHS ++ P G++
Sbjct: 188 AMIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKI 247
Query: 953 LATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQLVD 1078
+A E II AE+D ++E R ++PL+KQRR D+Y V+
Sbjct: 248 VAEAGEGEEIIYAELDPEVIESFRQAVPLTKQRRFDVYSDVN 289
>sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=Nit1 PE=2 SV=1
Length = 292
Score = 161 bits (406), Expect = 1e-038
Identities = 99/281 (35%), Positives = 147/281 (52%), Gaps = 21/281 (7%)
Frame = +2
Query: 257 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 436
+ +CQ++ T +K N + ++EA GA L LPE ++ N + + E G
Sbjct: 14 VAVCQVTSTPNKQENFKTCAELVQEATRLGACLAFLPEAFDFIARNPAETLLLSEPLDGD 73
Query: 437 VEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 601
+ S++++ GI + G ER D ++YN + S G + A +RK H
Sbjct: 74 L-----LGQYSQLARECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASYRKTH 128
Query: 602 LFDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 772
L D++IPG+ ES G E P V T G++G+ ICYD+RF EL++ A GA
Sbjct: 129 LCDVEIPGQGPMRESNYTMPGYALEPP--VKTPAGKVGLAICYDMRFPELSLKLAQAGAE 186
Query: 773 LLCYPGAFNMTTGPLHWELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPF 943
+L YP AF TGP HWE+L RARA ++Q YV A C ++ A Y GHS ++ P+
Sbjct: 187 ILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPW 243
Query: 944 GEVLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
G V+A+ + +A ID L+Q R LP+ + RR DLY
Sbjct: 244 GTVVASCSEGPGLCLARIDLHFLQQMRQHLPVFQHRRPDLY 284
>sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1 PE=1 SV=2
Length = 327
Score = 161 bits (405), Expect = 2e-038
Identities = 96/278 (34%), Positives = 146/278 (52%), Gaps = 15/278 (5%)
Frame = +2
Query: 257 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 436
+ +CQ++ T DK +N + + EAA GA L LPE ++ + + ++ E GG
Sbjct: 49 VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSE-PLGG 107
Query: 437 VEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 601
T ++++ G+ + G ER D ++YN + S G + A +RK H
Sbjct: 108 KLLEEYT----QLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTH 163
Query: 602 LFDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 772
L D++IPG+ ES + G E+P V T G+IG+ +CYD+RF EL++ A GA
Sbjct: 164 LCDVEIPGQGPMCESNSTMPGPSLESP--VSTPAGKIGLAVCYDMRFPELSLALAQAGAE 221
Query: 773 LLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEV 952
+L YP AF TGP HWE+L RARA + Q YV + ++GHS ++ P+G V
Sbjct: 222 ILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTV 281
Query: 953 LATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
+A + +A ID + L Q R LP+ + RR DLY
Sbjct: 282 VARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLY 319
>sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1 PE=2 SV=1
Length = 323
Score = 161 bits (405), Expect = 2e-038
Identities = 103/316 (32%), Positives = 158/316 (50%), Gaps = 24/316 (7%)
Frame = +2
Query: 146 SGLRSISSSSMASSFKPEQARVPSALPLPAPPLTKFNIGLCQLSVTADKTRNISHAKKAI 325
+G R + + + + +P ++ LP + +CQ++ T +K N + +
Sbjct: 15 TGYRLLRTPVLCTQPRPRTMSSSTSWELPL-------VAVCQVTSTPNKQENFKTCAELV 67
Query: 326 EEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGGVEASPSTAMLSEVSKRLGITIIG 505
+EAA GA L LPE ++ N + + E G + S++++ GI +
Sbjct: 68 QEAARLGACLAFLPEAFDFIARNPAETLLLSEPLNGDL-----LGQYSQLARECGIWLSL 122
Query: 506 GSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESK-TLTAGE 667
G ER D ++YN + S G + A +RK HL D++IPG+ ES T G
Sbjct: 123 GGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRESNYTKPGGT 182
Query: 668 TPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARA 847
V T G++G+ ICYD+RF EL++ A GA +L Y AF TGP HWE+L RARA
Sbjct: 183 LEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGAEILTYSSAFGSVTGPAHWEVLLRARA 242
Query: 848 TDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDYSILEQ 1018
++Q YV A C ++ A Y GHS ++ P+G V+A + +A ID L+Q
Sbjct: 243 IESQCYVIAAAQCGRHHETRASY---GHSMVVDPWGTVVARCSEGPGLCLARIDLHFLQQ 299
Query: 1019 RRTSLPLSKQRRGDLY 1066
R LP+ + RR DLY
Sbjct: 300 MRQHLPVFQHRRPDLY 315
>sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 PE=2 SV=1
Length = 328
Score = 158 bits (397), Expect = 1e-037
Identities = 93/278 (33%), Positives = 144/278 (51%), Gaps = 15/278 (5%)
Frame = +2
Query: 257 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDAGG 436
+ +CQ++ T DK +N + I EAA GA L LPE ++ + E +G
Sbjct: 50 VAVCQVTSTPDKEQNFKTCAELIREAARLGACLAFLPEAFDFIARDPEETRRLSEPLSGN 109
Query: 437 VEASPSTAMLSEVSKRLGITIIGGSIPERVGD-----RLYNTCCVFGSDGELKAKHRKIH 601
+ +++++ G+ + G ER D ++YN + + G + A +RK H
Sbjct: 110 L-----LEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVIMNNMGSVVATYRKTH 164
Query: 602 LFDIDIPGKITFMESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAH 772
L D++IPG+ ES + G E+P + T G+IG+ ICYD+RF EL++ GA
Sbjct: 165 LCDVEIPGQGPMRESNSTIPGPSLESP--ISTPAGKIGLAICYDMRFPELSLALVQAGAE 222
Query: 773 LLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEV 952
+L YP AF TGP HWE+L RARA + Q YV + ++GHS ++ P+G V
Sbjct: 223 ILTYPSAFGSVTGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTV 282
Query: 953 LATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLY 1066
+A + +A ID + L+Q R LP+ + RR DLY
Sbjct: 283 VARCSEGPGLCLARIDLNYLQQLRKQLPVFQHRRPDLY 320
>sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Caenorhabditis elegans GN=nft-1 PE=1 SV=1
Length = 440
Score = 151 bits (381), Expect = 1e-035
Identities = 77/179 (43%), Positives = 107/179 (59%), Gaps = 1/179 (0%)
Frame = +2
Query: 539 YNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAG-ETPTIVDTDVGRIGIGI 715
+NT + SDG +A++ K+HLFD++IPGK+ MES+ AG E VDT +GR+G+ I
Sbjct: 109 WNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGLSI 168
Query: 716 CYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDS 895
CYD+RF EL++ RGA LL +P AF + TG HWE L RARA +NQ YV +
Sbjct: 169 CYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAH 228
Query: 896 GAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
++GHS ++ P+G V+A + AEID S ++ R P+ RR DLY L
Sbjct: 229 NPKRQSYGHSMVVDPWGAVVAQCSERVDMCFAEIDLSYVDTLREMQPVFSHRRSDLYTL 287
>sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Drosophila melanogaster GN=NitFhit PE=1 SV=1
Length = 460
Score = 142 bits (356), Expect = 8e-033
Identities = 82/209 (39%), Positives = 118/209 (56%), Gaps = 7/209 (3%)
Frame = +2
Query: 458 AMLSEVSKRLGITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITF 637
A E++K I I G + ER +++N + GEL A +RK+H+FD+ ++
Sbjct: 97 AQYRELAKCNKIWISLGGVHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTK-EVRL 155
Query: 638 MESKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTT 808
ES T+T G E P V T VG+IG+ ICYD+RF E A++ GA+LL YP AF T
Sbjct: 156 RESDTVTPGYCLERP--VSTPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYAT 213
Query: 809 GPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLA-TTEHEEAII 985
G HWE+L RARA + Q +V + +WGHS ++ P+G VLA +E E I
Sbjct: 214 GKAHWEILLRARAIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIG 273
Query: 986 IAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
AE+D S+L+ ++P + RR D+Y L
Sbjct: 274 TAEVDLSVLQSLYQTMPCFEHRRNDIYAL 302
>sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoideum GN=nit1-1
PE=3 SV=1
Length = 291
Score = 140 bits (352), Expect = 2e-032
Identities = 90/286 (31%), Positives = 149/286 (52%), Gaps = 23/286 (8%)
Frame = +2
Query: 257 IGLCQLSVTADKTRNISHAKKAIEEAASKGAKLVLLPEIW-------NSPYSNDSFPVYA 415
IGL Q++ T +K N K+ IE+A L LPE + + S D+ A
Sbjct: 15 IGLGQITSTNNKEDNFRKCKEMIEKAVENKVNLFCLPECFAFISGGIHQFESRDN----A 70
Query: 416 EEID-AGGVEASPSTAMLSEVSKRLGITIIGGSIPERV----GDRLYNTCCVFGSDGELK 580
E +D GG+ +++K+ I + G E++ D +YNT + S+G +
Sbjct: 71 EYLDQKGGI-----IERYKDLAKQNNIWLSLGGFHEKILDDPNDMIYNTHLIIDSNGVIV 125
Query: 581 AKHRKIHLFDIDIPGK-ITFMESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYA 757
++RK+HLFD+DIP K + ESK + G + D+ VG++G+ ICYD+RF EL +
Sbjct: 126 CEYRKMHLFDVDIPSKGVKMNESKVVKGGNDLVVCDSPVGKLGLSICYDLRFPELYLSLR 185
Query: 758 ARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIG 937
A +L P AF +TG HW+ L +ARA +NQ YV + D + +++GHS +I
Sbjct: 186 RMDAQILLVPSAFMKSTGEAHWKPLLQARAIENQTYVIAAAQTGDHHSKRSSYGHSMIID 245
Query: 938 PFGEVL-ATTEHEEAIIIAEIDYSILEQRRTSLPLSKQRRGDLYQL 1072
P+G+VL ++ I +ID + R ++P+ ++ + Y++
Sbjct: 246 PWGKVLHDLPDNLNDIAFVDIDLDYISTCRENIPVFNHKKLNNYKI 291
>sp|O94660|NIT2_SCHPO Probable hydrolase nit2 OS=Schizosaccharomyces pombe
GN=nit2 PE=2 SV=1
Length = 276
Score = 120 bits (300), Expect = 3e-026
Identities = 69/192 (35%), Positives = 105/192 (54%), Gaps = 7/192 (3%)
Frame = +2
Query: 515 PERVGDRLYNTCC-VFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAGET-PTIVDT 688
P +V ++L N+ + GE+ +++ K HLFD++I T ES T GE T
Sbjct: 87 PSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKESNTTLRGEAILPPCKT 146
Query: 689 DVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYV 868
+G++G IC+DIRF E A+ GAH++ YP AF TG HWE+L RARA D+Q YV
Sbjct: 147 PLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAAHWEVLLRARALDSQCYV 206
Query: 869 ATCSPARDSGAGYTAWGHSTLIGPFGEVLA---TTEHEEAIIIAEIDYSILEQRRTSLPL 1039
+ ++GHS ++ P+G V+A +I A++D ++++ RT +PL
Sbjct: 207 IAPAQGGKHNEKRASYGHSMIVDPWGTVIAQYSDISSPNGLIFADLDLNLVDHVRTYIPL 266
Query: 1040 SKQRRGDLYQLV 1075
RR DLY +
Sbjct: 267 --LRRNDLYPTI 276
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 164,931,892,944
Number of Sequences: 518415
Number of Extensions: 164931892944
Number of Successful Extensions: 1021520109
Number of sequences better than 0.0: 0
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