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TrEMBL blast output of UN44134


BLASTX 7.6.2

Query= UN44134 /QuerySize=837
        (836 letters)

Database: UniProt/TrEMBL;
          11,397,958 sequences; 3,661,877,547 total letters
                                                                  Score    E
Sequences producing significant alignments:                       (bits) Value

tr|Q8RYC3|Q8RYC3_ARATH Expressed protein OS=Arabidopsis thaliana...    177   1e-042
tr|A9P929|A9P929_POPTR Putative uncharacterized protein OS=Popul...    154   1e-035
tr|B9HZQ6|B9HZQ6_POPTR Predicted protein OS=Populus trichocarpa ...    153   2e-035
tr|A9PCG5|A9PCG5_POPTR Putative uncharacterized protein OS=Popul...    151   8e-035
tr|B6SHT0|B6SHT0_MAIZE OB-fold nucleic acid binding domain conta...    151   8e-035
tr|B9N1D5|B9N1D5_POPTR Predicted protein (Fragment) OS=Populus t...    151   8e-035
tr|Q5JML8|Q5JML8_ORYSJ DNA-binding protein-related-like OS=Oryza...    151   8e-035
tr|A2WML6|A2WML6_ORYSI Putative uncharacterized protein OS=Oryza...    149   3e-034
tr|B9RBA3|B9RBA3_RICCO Putative uncharacterized protein OS=Ricin...    149   3e-034
tr|C5XJI8|C5XJI8_SORBI Putative uncharacterized protein Sb03g000...    149   3e-034
tr|Q9FTU2|Q9FTU2_ORYSJ Os01g0238600 protein OS=Oryza sativa subs...    149   4e-034
tr|B4FR03|B4FR03_MAIZE OB-fold nucleic acid binding domain conta...    149   5e-034
tr|B8A9R0|B8A9R0_ORYSI Putative uncharacterized protein OS=Oryza...    148   7e-034
tr|B9SHB1|B9SHB1_RICCO Putative uncharacterized protein OS=Ricin...    147   2e-033
tr|A9NK64|A9NK64_PICSI Putative uncharacterized protein OS=Picea...    145   6e-033
tr|A5C3Z4|A5C3Z4_VITVI Putative uncharacterized protein OS=Vitis...    144   1e-032
tr|B9RVU7|B9RVU7_RICCO Putative uncharacterized protein OS=Ricin...    144   1e-032
tr|C6T2Y3|C6T2Y3_SOYBN Putative uncharacterized protein OS=Glyci...    144   2e-032
tr|A9PCK0|A9PCK0_POPTR Predicted protein OS=Populus trichocarpa ...    143   2e-032
tr|A5BEE0|A5BEE0_VITVI Putative uncharacterized protein OS=Vitis...    143   4e-032

>tr|Q8RYC3|Q8RYC3_ARATH Expressed protein OS=Arabidopsis thaliana GN=At2g33845
        PE=2 SV=1

          Length = 182

 Score =  177 bits (448), Expect = 1e-042
 Identities = 99/155 (63%), Positives = 109/155 (70%), Gaps = 3/155 (1%)
 Frame = -1

Query: 713 GASPKTPPKK-RTSSSSPPRKKPAASLSP--DDGHGHCSGSSGEEETRLRQGRSSQKPNA 543
           G +P  P    RTS + PP+  P     P          G+SG   T     ++S     
Sbjct:  27 GRNPPPPSSSTRTSQNQPPQHTPQEKKKPVFVKVDQLKPGTSGHTLTVKVVDQNSVPQKP 86

Query: 542 AAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLT 363
            AA+SSHLRP RISECLVGDETA ILFTARNDQV+LMKPGATVNLRNAKIDMFKGSMRL 
Sbjct:  87 GAASSSHLRPARISECLVGDETACILFTARNDQVELMKPGATVNLRNAKIDMFKGSMRLA 146

Query: 362 VDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           VDKWGRIE TEP ++TVKEDNNLSLVEYELVNV E
Sbjct: 147 VDKWGRIEATEPADITVKEDNNLSLVEYELVNVVE 181

>tr|A9P929|A9P929_POPTR Putative uncharacterized protein OS=Populus trichocarpa
        PE=2 SV=1

          Length = 155

 Score =  154 bits (388), Expect = 1e-035
 Identities = 74/93 (79%), Positives = 81/93 (87%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           + S H+R  RI+ECL+GDET +I+FTARNDQVDLMKPG TV LRNAKIDMFKGSMRL VD
Sbjct:  62 SVSLHVRQTRIAECLIGDETGTIIFTARNDQVDLMKPGTTVILRNAKIDMFKGSMRLAVD 121

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           KWGR+EVTEP E  VKEDNNLSLVEYELVNV E
Sbjct: 122 KWGRVEVTEPAEFVVKEDNNLSLVEYELVNVAE 154

>tr|B9HZQ6|B9HZQ6_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_1094220 PE=4 SV=1

          Length = 155

 Score =  153 bits (386), Expect = 2e-035
 Identities = 74/93 (79%), Positives = 80/93 (86%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           + S H R  RI+ECL+GDET +I+FTARNDQVDLMKPG TV LRNAKIDMFKGSMRL VD
Sbjct:  62 SVSLHARQTRIAECLIGDETGTIIFTARNDQVDLMKPGTTVILRNAKIDMFKGSMRLAVD 121

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           KWGR+EVTEP E  VKEDNNLSLVEYELVNV E
Sbjct: 122 KWGRVEVTEPAEFVVKEDNNLSLVEYELVNVAE 154

>tr|A9PCG5|A9PCG5_POPTR Putative uncharacterized protein OS=Populus trichocarpa
        PE=2 SV=1

          Length = 153

 Score =  151 bits (381), Expect = 8e-035
 Identities = 72/91 (79%), Positives = 81/91 (89%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           + S HLR +RI+ECL+GD+T SI+FTARN+QVDL+KPG TV LRNAKIDMFKGSMRL VD
Sbjct:  60 SVSHHLRQSRIAECLIGDDTGSIIFTARNEQVDLVKPGTTVILRNAKIDMFKGSMRLAVD 119

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNV 264
           KWGR+EVTEP E  VKEDNNLSLVEYELVNV
Sbjct: 120 KWGRVEVTEPAEFVVKEDNNLSLVEYELVNV 150

>tr|B6SHT0|B6SHT0_MAIZE OB-fold nucleic acid binding domain containing protein
        OS=Zea mays PE=2 SV=1

          Length = 146

 Score =  151 bits (381), Expect = 8e-035
 Identities = 79/99 (79%), Positives = 83/99 (83%), Gaps = 2/99 (2%)
 Frame = -1

Query: 554 KPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGS 375
           +P A AAASS  R  RI+ECLVGDET  I+FTARNDQVDL+KP ATV LRNAKIDMFKGS
Sbjct:  50 RPGAPAAASS--RAPRIAECLVGDETGVIVFTARNDQVDLLKPDATVILRNAKIDMFKGS 107

Query: 374 MRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           MRL VDKWGRIE TEP   TVKEDNNLSLVEYELVNV E
Sbjct: 108 MRLAVDKWGRIEATEPASFTVKEDNNLSLVEYELVNVAE 146

>tr|B9N1D5|B9N1D5_POPTR Predicted protein (Fragment) OS=Populus trichocarpa
        GN=POPTRDRAFT_263374 PE=4 SV=1

          Length = 134

 Score =  151 bits (381), Expect = 8e-035
 Identities = 72/91 (79%), Positives = 81/91 (89%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           + S HLR +RI+ECL+GD+T SI+FTARN+QVDL+KPG TV LRNAKIDMFKGSMRL VD
Sbjct:  43 SVSHHLRQSRIAECLIGDDTGSIIFTARNEQVDLVKPGTTVILRNAKIDMFKGSMRLAVD 102

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNV 264
           KWGR+EVTEP E  VKEDNNLSLVEYELVNV
Sbjct: 103 KWGRVEVTEPAEFVVKEDNNLSLVEYELVNV 133

>tr|Q5JML8|Q5JML8_ORYSJ DNA-binding protein-related-like OS=Oryza sativa subsp.
        japonica GN=P0503C12.16 PE=2 SV=1

          Length = 140

 Score =  151 bits (381), Expect = 8e-035
 Identities = 76/99 (76%), Positives = 81/99 (81%)
 Frame = -1

Query: 557 QKPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKG 378
           QK  AAA A    RP RI+ECL+GDET  ILFTARN+QVDLMK  +TV +RNAKIDMFKG
Sbjct:  41 QKGRAAAGAGPAARPTRIAECLIGDETGCILFTARNEQVDLMKADSTVIIRNAKIDMFKG 100

Query: 377 SMRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVE 261
           SMRL VDKWGRIEVTEP    VKEDNNLSLVEYELVNVE
Sbjct: 101 SMRLAVDKWGRIEVTEPASFNVKEDNNLSLVEYELVNVE 139

>tr|A2WML6|A2WML6_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
        indica GN=OsI_01083 PE=4 SV=1

          Length = 141

 Score =  149 bits (376), Expect = 3e-034
 Identities = 75/99 (75%), Positives = 83/99 (83%), Gaps = 4/99 (4%)
 Frame = -1

Query: 554 KPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGS 375
           +P  AA +    RP RI+ECLVGDET +I+FTARN+QVDL+KPGATV +RNAKIDMFKGS
Sbjct:  47 RPGVAAPS----RPPRIAECLVGDETGAIVFTARNEQVDLLKPGATVIMRNAKIDMFKGS 102

Query: 374 MRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           MRL VDKWGRIE TEP   TVKEDNNLSLVEYELVNV E
Sbjct: 103 MRLAVDKWGRIEATEPASFTVKEDNNLSLVEYELVNVTE 141

>tr|B9RBA3|B9RBA3_RICCO Putative uncharacterized protein OS=Ricinus communis
        GN=RCOM_1673610 PE=4 SV=1

          Length = 150

 Score =  149 bits (376), Expect = 3e-034
 Identities = 78/108 (72%), Positives = 86/108 (79%), Gaps = 7/108 (6%)
 Frame = -1

Query: 587 ETRLRQGRSSQKPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNL 408
           +T L +GR       A A+S+ LR  RI+ECL+GDET +ILFTARNDQVDLMKP  TV L
Sbjct:  46 DTVLHKGR-------ATASSNRLRNTRIAECLIGDETGTILFTARNDQVDLMKPETTVIL 98

Query: 407 RNAKIDMFKGSMRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNV 264
           RNAKIDMFKGSMRL VDKWGRIEVTEP +  VKEDNNLSLVEYELV V
Sbjct:  99 RNAKIDMFKGSMRLAVDKWGRIEVTEPAKFAVKEDNNLSLVEYELVTV 146

>tr|C5XJI8|C5XJI8_SORBI Putative uncharacterized protein Sb03g000440 OS=Sorghum
        bicolor GN=Sb03g000440 PE=4 SV=1

          Length = 146

 Score =  149 bits (376), Expect = 3e-034
 Identities = 77/99 (77%), Positives = 82/99 (82%), Gaps = 2/99 (2%)
 Frame = -1

Query: 554 KPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGS 375
           +P A AA  S  R  RI+ECLVGDET +I+FTARNDQVDL+KP ATV LRNAKIDMFKGS
Sbjct:  50 RPGAPAAVPS--RAPRIAECLVGDETGAIVFTARNDQVDLLKPNATVILRNAKIDMFKGS 107

Query: 374 MRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           MRL VDKWGRIE TEP   TVKEDNNLSLVEYELVNV E
Sbjct: 108 MRLAVDKWGRIEATEPASFTVKEDNNLSLVEYELVNVAE 146

>tr|Q9FTU2|Q9FTU2_ORYSJ Os01g0238600 protein OS=Oryza sativa subsp. japonica
        GN=OSJNBa0086P08.24 PE=2 SV=1

          Length = 141

 Score =  149 bits (375), Expect = 4e-034
 Identities = 74/99 (74%), Positives = 83/99 (83%), Gaps = 4/99 (4%)
 Frame = -1

Query: 554 KPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGS 375
           +P  AA +    RP RI+ECL+GDET +I+FTARN+QVDL+KPGATV +RNAKIDMFKGS
Sbjct:  47 RPGVAAPS----RPPRIAECLIGDETGAIVFTARNEQVDLLKPGATVIMRNAKIDMFKGS 102

Query: 374 MRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           MRL VDKWGRIE TEP   TVKEDNNLSLVEYELVNV E
Sbjct: 103 MRLAVDKWGRIEATEPASFTVKEDNNLSLVEYELVNVTE 141

>tr|B4FR03|B4FR03_MAIZE OB-fold nucleic acid binding domain containing protein
        OS=Zea mays PE=2 SV=1

          Length = 146

 Score =  149 bits (374), Expect = 5e-034
 Identities = 76/99 (76%), Positives = 82/99 (82%), Gaps = 2/99 (2%)
 Frame = -1

Query: 554 KPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGS 375
           +P A AAA S  R  RI+ECLVGDET +I+FTARNDQVDL+KP ATV LRNAKIDMFKGS
Sbjct:  50 RPGAPAAAPS--RAPRIAECLVGDETGAIVFTARNDQVDLLKPNATVILRNAKIDMFKGS 107

Query: 374 MRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           MRL VDKWGRIE  EP   TVKEDNNLSL+EYELVNV E
Sbjct: 108 MRLAVDKWGRIEAVEPASFTVKEDNNLSLIEYELVNVAE 146

>tr|B8A9R0|B8A9R0_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
        indica GN=OsI_03774 PE=4 SV=1

          Length = 140

 Score =  148 bits (373), Expect = 7e-034
 Identities = 76/109 (69%), Positives = 87/109 (79%), Gaps = 4/109 (3%)
 Frame = -1

Query: 587 ETRLRQGRSSQKPNAAAAASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNL 408
           +T +++GR++    A A A    RP RI+ECL+GDET  ILFTARN+QVDLMK  +TV +
Sbjct:  35 KTVVQKGRAA----AGAGAGPAARPTRIAECLIGDETGCILFTARNEQVDLMKADSTVII 90

Query: 407 RNAKIDMFKGSMRLTVDKWGRIEVTEPVEMTVKEDNNLSLVEYELVNVE 261
           RNAKIDMFKGSMRL VDKWGRIEVTEP    VKEDNNLSLVEYELVNVE
Sbjct:  91 RNAKIDMFKGSMRLAVDKWGRIEVTEPASFNVKEDNNLSLVEYELVNVE 139

>tr|B9SHB1|B9SHB1_RICCO Putative uncharacterized protein OS=Ricinus communis
        GN=RCOM_0528470 PE=4 SV=1

          Length = 136

 Score =  147 bits (370), Expect = 2e-033
 Identities = 72/93 (77%), Positives = 78/93 (83%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           A    +R  RI+ECLVGDET  I+FTARNDQVDLMK G+TV LRNAKIDMFKGSMRL VD
Sbjct:  43 ADGPQIRQMRIAECLVGDETGMIIFTARNDQVDLMKEGSTVTLRNAKIDMFKGSMRLAVD 102

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           KWGR+EVTEP   TVKEDNNLSL+EYELVNV E
Sbjct: 103 KWGRVEVTEPASFTVKEDNNLSLIEYELVNVVE 135

>tr|A9NK64|A9NK64_PICSI Putative uncharacterized protein OS=Picea sitchensis
        PE=2 SV=1

          Length = 140

 Score =  145 bits (365), Expect = 6e-033
 Identities = 70/90 (77%), Positives = 80/90 (88%)
 Frame = -1

Query: 527 SHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVDKWG 348
           S +R  RI+ECL+GDET  ++FTARN+QVDLMK G+TV LRNAKIDMFKGSMRL VDKWG
Sbjct:  50 SQVRQMRIAECLIGDETGIVIFTARNEQVDLMKEGSTVILRNAKIDMFKGSMRLAVDKWG 109

Query: 347 RIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           RIEVTEP E +VKE+NNLSLVEYELVNV+E
Sbjct: 110 RIEVTEPAEFSVKEENNLSLVEYELVNVQE 139

>tr|A5C3Z4|A5C3Z4_VITVI Putative uncharacterized protein OS=Vitis vinifera
        GN=VITISV_018558 PE=4 SV=1

          Length = 137

 Score =  144 bits (362), Expect = 1e-032
 Identities = 71/88 (80%), Positives = 77/88 (87%)
 Frame = -1

Query: 521 LRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVDKWGRI 342
           +R  RI+ECLVGDET  I+FTARNDQVDLMK G+TV LRNAKIDMFKGSMRL VDKWGR+
Sbjct:  49 VRQMRIAECLVGDETGMIIFTARNDQVDLMKEGSTVVLRNAKIDMFKGSMRLAVDKWGRV 108

Query: 341 EVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           EVTEP   +VKEDNNLSLVEYELVNV E
Sbjct: 109 EVTEPASFSVKEDNNLSLVEYELVNVVE 136

>tr|B9RVU7|B9RVU7_RICCO Putative uncharacterized protein OS=Ricinus communis
        GN=RCOM_1173010 PE=4 SV=1

          Length = 140

 Score =  144 bits (362), Expect = 1e-032
 Identities = 71/86 (82%), Positives = 76/86 (88%)
 Frame = -1

Query: 518 RPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVDKWGRIE 339
           RP RISECLVGDETASI+FTARN+QVDLM PGATV LRNAKIDMFKGSMRL VDKWGRIE
Sbjct:  54 RPARISECLVGDETASIVFTARNEQVDLMNPGATVILRNAKIDMFKGSMRLAVDKWGRIE 113

Query: 338 VTEPVEMTVKEDNNLSLVEYELVNVE 261
            TEP +  V+E NNLSLVEYELV V+
Sbjct: 114 ATEPADFVVQETNNLSLVEYELVTVQ 139

>tr|C6T2Y3|C6T2Y3_SOYBN Putative uncharacterized protein OS=Glycine max PE=2
        SV=1

          Length = 137

 Score =  144 bits (361), Expect = 2e-032
 Identities = 69/85 (81%), Positives = 74/85 (87%)
 Frame = -1

Query: 518 RPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVDKWGRIE 339
           R  RI+ECLVGDET  I+FTARNDQVD+MK GATV LRNAKIDMFKGSMRL  DKWGR+E
Sbjct:  50 RQMRIAECLVGDETGMIIFTARNDQVDMMKEGATVTLRNAKIDMFKGSMRLAADKWGRVE 109

Query: 338 VTEPVEMTVKEDNNLSLVEYELVNV 264
           VTEP   TVKEDNNLSL+EYELVNV
Sbjct: 110 VTEPASFTVKEDNNLSLIEYELVNV 134

>tr|A9PCK0|A9PCK0_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_814065 PE=2 SV=1

          Length = 138

 Score =  143 bits (360), Expect = 2e-032
 Identities = 71/93 (76%), Positives = 78/93 (83%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           A    +R  RI+ECLVGDET  I+FTARNDQVDLMK  +TV LRNAKIDMFKGSMRL VD
Sbjct:  45 ADGPQVRQMRIAECLVGDETGLIIFTARNDQVDLMKEDSTVILRNAKIDMFKGSMRLAVD 104

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           KWGR+EVTEP + TVKEDNNLSL+EYELVNV E
Sbjct: 105 KWGRVEVTEPADFTVKEDNNLSLIEYELVNVVE 137

>tr|A5BEE0|A5BEE0_VITVI Putative uncharacterized protein OS=Vitis vinifera
        GN=VITISV_018007 PE=4 SV=1

          Length = 130

 Score =  143 bits (358), Expect = 4e-032
 Identities = 69/93 (74%), Positives = 76/93 (81%)
 Frame = -1

Query: 536 AASSHLRPNRISECLVGDETASILFTARNDQVDLMKPGATVNLRNAKIDMFKGSMRLTVD 357
           + S HLR  RI+ECLVGDET +I+FTARNDQVD+MKPG TV L  AKIDMFKGSMRL +D
Sbjct:  37 SVSQHLRHTRIAECLVGDETGAIIFTARNDQVDMMKPGTTVILHKAKIDMFKGSMRLVID 96

Query: 356 KWGRIEVTEPVEMTVKEDNNLSLVEYELVNVEE 258
           KWGR+EVTE     VKE NNLSLVEYELVNV E
Sbjct:  97 KWGRVEVTEDANFVVKEQNNLSLVEYELVNVLE 129

  Database: UniProt/TrEMBL
    Posted date:  Sat Aug 07 14:51:12 2010
  Number of letters in database: 3,661,877,547
  Number of sequences in database:  11,397,958

Lambda     K     H
   0.267   0.041    0.140
Gapped
Lambda     K     H
   0.267   0.041    0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,328,092,471,232
Number of Sequences: 11397958
Number of Extensions: 3328092471232
Number of Successful Extensions: 1153085747
Number of sequences better than 0.0: 0