BLASTX 7.6.2
Query= UN44507 /QuerySize=1208
(1207 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 121 2e-026
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 90 3e-017
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 85 1e-015
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 85 1e-015
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 85 1e-015
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 82 9e-015
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 82 9e-015
sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=... 77 2e-013
sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sap... 73 3e-012
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 61 2e-008
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 54 2e-006
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 53 4e-006
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 53 4e-006
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 121 bits (301), Expect = 2e-026
Identities = 66/218 (30%), Positives = 106/218 (48%), Gaps = 7/218 (3%)
Frame = +2
Query: 143 NLTWLETVWFDYSATKSDYYLYCHNILFLFLVFSLAP-LPLVLVELARSASGWFDRYKIQ 319
+L ++E WF + + +L + VF +P L + + +YKIQ
Sbjct: 5 SLKFIEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDF----MPFLQKYKIQ 60
Query: 320 PKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGIRSGSPLPTFSEMLPQLFVY 499
P F C V+ I P+ + P+I+ IG+ + +PLP+ ++ +
Sbjct: 61 PTKKNEWKTQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACC 120
Query: 500 FLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAI 679
FLIED+ YWVHR H + Y++IH+VHH++ AP G A YAH E ++LG+ T +GP +
Sbjct: 121 FLIEDFYFYWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFL 180
Query: 680 APGHMITFWLWIALRQIGAIE--STI*FSMESNKIHPF 787
+ T W+W+ R +E S F K+ PF
Sbjct: 181 FSRDLFTLWVWLGTRLFQTVECHSGYDFPWNPTKLIPF 218
Score = 64 bits (155), Expect = 1e-009
Identities = 29/59 (49%), Positives = 40/59 (67%), Gaps = 7/59 (11%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYHYYHH--YVGGQSHSNLASVFTYCDYIYGTDKGYRFQKKL 920
YDFPW+PTK IPF+GG+ +H +HH +VG N +S FTY D I+GT Y +K++
Sbjct: 206 YDFPWNPTKLIPFWGGSHFHDFHHETFVG-----NYSSTFTYLDKIFGTSDKYYSRKQI 259
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 90 bits (222), Expect = 3e-017
Identities = 49/163 (30%), Positives = 80/163 (49%), Gaps = 1/163 (0%)
Frame = +2
Query: 296 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGI-RSGSPLPTFS 472
+ +YKIQP + + C+K ++ PL +Y + I +P +
Sbjct: 77 YMQKYKIQPDKPETWEKQWKCFKTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWY 136
Query: 473 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 652
+L Q F +IED +Y++HR H K Y+ IH+VHHE+ +P G A YAH E L+LG
Sbjct: 137 VLLAQCFGCAVIEDAWHYFLHRLLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILG 196
Query: 653 IPTFMGPAIAPGHMITFWLWIALRQIGAIESTI*FSMESNKIH 781
F+G + H++ W W+ R + I+ + + N +H
Sbjct: 197 AGFFIGIVVFCNHVVLLWAWVICRLMETIDVHSGYDIPLNPLH 239
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 85 bits (208), Expect = 1e-015
Identities = 47/150 (31%), Positives = 76/150 (50%), Gaps = 1/150 (0%)
Frame = +2
Query: 296 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGI-RSGSPLPTFS 472
+ +YKIQ + + + C+K ++ PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 473 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 652
+L + F +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 653 IPTFMGPAIAPGHMITFWLWIALRQIGAIE 742
F+G + H+I W W+ +R + I+
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETID 226
Score = 52 bits (122), Expect = 1e-005
Identities = 27/66 (40%), Positives = 37/66 (56%), Gaps = 15/66 (22%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYHYYHH--YVGGQSHSNLASVFTYCDYIYGTDKGY------- 902
YD P +P IPFY G+ +H +HH ++G N AS FT+ D I+GTD Y
Sbjct: 231 YDIPLNPLNLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDSQYNAYNEKR 285
Query: 903 -RFQKK 917
+F+KK
Sbjct: 286 KKFEKK 291
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 85 bits (208), Expect = 1e-015
Identities = 47/150 (31%), Positives = 76/150 (50%), Gaps = 1/150 (0%)
Frame = +2
Query: 296 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGI-RSGSPLPTFS 472
+ +YKIQ + + + C+K ++ PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 473 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 652
+L + F +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFMHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 653 IPTFMGPAIAPGHMITFWLWIALRQIGAIE 742
F+G + H+I W W+ +R + I+
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETID 226
Score = 52 bits (123), Expect = 8e-006
Identities = 26/60 (43%), Positives = 36/60 (60%), Gaps = 9/60 (15%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYHYYHH--YVGGQSHSNLASVFTYCDYIYGTDKGYR--FQKK 917
YD P +P IPFY G+ +H +HH ++G N AS FT+ D I+GTD Y ++KK
Sbjct: 231 YDIPLNPLNLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDSQYHAYYEKK 285
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 85 bits (208), Expect = 1e-015
Identities = 47/150 (31%), Positives = 76/150 (50%), Gaps = 1/150 (0%)
Frame = +2
Query: 296 WFDRYKIQPKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGI-RSGSPLPTFS 472
+ +YKIQ + + + C+K ++ PL +Y + I +P +
Sbjct: 77 YMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWY 136
Query: 473 EMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLG 652
+L + F +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 137 FLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILG 196
Query: 653 IPTFMGPAIAPGHMITFWLWIALRQIGAIE 742
F+G + H+I W W+ +R + I+
Sbjct: 197 TGFFIGIVLLCDHVILLWAWVTIRLLETID 226
Score = 52 bits (122), Expect = 1e-005
Identities = 27/66 (40%), Positives = 37/66 (56%), Gaps = 15/66 (22%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYHYYHH--YVGGQSHSNLASVFTYCDYIYGTDKGY------- 902
YD P +P IPFY G+ +H +HH ++G N AS FT+ D I+GTD Y
Sbjct: 231 YDIPLNPLNLIPFYAGSRHHDFHHMNFIG-----NYASTFTWWDRIFGTDSQYNAYNEKR 285
Query: 903 -RFQKK 917
+F+KK
Sbjct: 286 KKFEKK 291
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 82 bits (200), Expect = 9e-015
Identities = 46/147 (31%), Positives = 71/147 (48%), Gaps = 1/147 (0%)
Frame = +2
Query: 305 RYKIQPKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGI-RSGSPLPTFSEML 481
+YKIQ + + C K ++ PL +Y + I +P + L
Sbjct: 80 KYKIQKDKPETFEGQWKCLKKILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYLTL 139
Query: 482 PQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPT 661
+ +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 140 ARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGF 199
Query: 662 FMGPAIAPGHMITFWLWIALRQIGAIE 742
F+G + H+I W W+ +R + I+
Sbjct: 200 FIGIVLLCDHVILLWAWVTIRLLETID 226
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 82 bits (200), Expect = 9e-015
Identities = 46/147 (31%), Positives = 71/147 (48%), Gaps = 1/147 (0%)
Frame = +2
Query: 305 RYKIQPKVNYSLSDMFGCYKDVMKMFILAVGPLQLVSYPSIQMIGI-RSGSPLPTFSEML 481
+YKIQ + + C K ++ PL +Y + I +P + L
Sbjct: 80 KYKIQKDKPETFEGQWKCLKGILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYFTL 139
Query: 482 PQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPT 661
+ +IED +Y++HR H K Y+ IH+VHHE+ AP G A YAH E L+LG
Sbjct: 140 ARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGF 199
Query: 662 FMGPAIAPGHMITFWLWIALRQIGAIE 742
F+G + H+I W W+ +R + I+
Sbjct: 200 FIGIVLLCDHVILLWAWVTMRLLETID 226
Score = 54 bits (128), Expect = 2e-006
Identities = 26/53 (49%), Positives = 33/53 (62%), Gaps = 7/53 (13%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYHYYHH--YVGGQSHSNLASVFTYCDYIYGTDKGY 902
YD P +P YIPFY GA +H +HH ++G N AS FT+ D I+GTD Y
Sbjct: 231 YDIPLNPLNYIPFYTGARHHDFHHMNFIG-----NYASTFTWWDRIFGTDVQY 278
>sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=Macaca
fascicularis GN=QccE-20373 PE=2 SV=1
Length = 333
Score = 77 bits (189), Expect = 2e-013
Identities = 49/160 (30%), Positives = 78/160 (48%), Gaps = 3/160 (1%)
Frame = +2
Query: 260 LVLVELARSASGWFDRYKIQPKVNYSLS--DMFGCYKDVMKMFILAVGPLQLVSYPSIQM 433
L+LV + RY+IQ N + + + V+ + P+ + YP ++
Sbjct: 97 LLLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMVSFPMVVFLYPFLKW 156
Query: 434 IGIRSGSPLPTFSEMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYA 613
G LPTF L +L ++ LIE+ Y+ HR H Y+ IH+ HHE+TAPIG
Sbjct: 157 WGDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVI 216
Query: 614 APYAHWAE-VLLLGIPTFMGPAIAPGHMITFWLWIALRQI 730
+ YAH E V+ +P +GP + H+ + +W +L I
Sbjct: 217 SLYAHPIEHVVSNMLPAIVGPLVMGSHLSSITMWFSLALI 256
>sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sapiens GN=C5orf4
PE=1 SV=1
Length = 333
Score = 73 bits (178), Expect = 3e-012
Identities = 47/160 (29%), Positives = 76/160 (47%), Gaps = 3/160 (1%)
Frame = +2
Query: 260 LVLVELARSASGWFDRYKIQPKVNYSLS--DMFGCYKDVMKMFILAVGPLQLVSYPSIQM 433
L+LV + RY+IQ N + + + V+ + P+ + YP ++
Sbjct: 97 LLLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKW 156
Query: 434 IGIRSGSPLPTFSEMLPQLFVYFLIEDYTNYWVHRFFHSKWGYENIHRVHHEYTAPIGYA 613
LPTF L +L ++ LIE+ Y+ HR H Y+ IH+ HHE+TAPIG
Sbjct: 157 WRDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVI 216
Query: 614 APYAHWAEVLLLG-IPTFMGPAIAPGHMITFWLWIALRQI 730
+ YAH E + +P +GP + H+ + +W +L I
Sbjct: 217 SLYAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLALI 256
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 61 bits (146), Expect = 2e-008
Identities = 33/63 (52%), Positives = 39/63 (61%), Gaps = 6/63 (9%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYHYYHHYVGGQSHSNLASVFTYCDYIYGTD-KGY--RFQKKL 920
YDFPWS T IPF+GGA +H YHH V N AS FTY D I+GT K Y R +KK
Sbjct: 206 YDFPWSFTNLIPFWGGAPFHDYHHEV---FIGNYASTFTYLDKIFGTSGKSYYSRIEKKS 262
Query: 921 LEQ 929
+ +
Sbjct: 263 INK 265
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 54 bits (129), Expect = 2e-006
Identities = 25/72 (34%), Positives = 46/72 (63%), Gaps = 7/72 (9%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYH--YYHHYVGGQSHSNLASVFTYCDYIYGTDKGYRFQKKLL 923
Y+FPWS ++PF+ GA++H ++H+++GG S S F + D+I T+ G + +K
Sbjct: 239 YEFPWSLHNFLPFWAGADHHDEHHHYFIGGYSSS-----FRWWDFILDTEAGPKAKKGRE 293
Query: 924 EQVKESSKKSNK 959
++VK++ +K K
Sbjct: 294 DKVKQNVEKLQK 305
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 53 bits (125), Expect = 4e-006
Identities = 27/72 (37%), Positives = 43/72 (59%), Gaps = 5/72 (6%)
Frame = +3
Query: 744 AQYDFPWSPTKYIPFYGGAEYHYYHHYVGGQSHSNLASVFTYCDYIYGTDKGY-RFQ-KK 917
A YDFPWS K++P + GA++H YHH N +S F + D + TD+ Y +F+ ++
Sbjct: 231 AGYDFPWSLNKFLPIWAGADHHDYHHMA---FKDNFSSSFRWWDAVLKTDQNYHQFKARR 287
Query: 918 LLEQVKESSKKS 953
L + + SKK+
Sbjct: 288 LAAKYEAESKKA 299
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 53 bits (125), Expect = 4e-006
Identities = 27/69 (39%), Positives = 40/69 (57%), Gaps = 5/69 (7%)
Frame = +3
Query: 750 YDFPWSPTKYIPFYGGAEYH-YYHHYVGGQSHSNLASVFTYCDYIYGTDKGYRFQKKLLE 926
YDFPWS K +PF+ GAE+H +HHY G N AS F + DY T+ G + E
Sbjct: 239 YDFPWSLNKIMPFWAGAEHHDLHHHYFIG----NYASSFRWWDYCLDTESGPEAKASREE 294
Query: 927 QVKESSKKS 953
++K+ ++ +
Sbjct: 295 RMKKRAENN 303
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 167,919,208,508
Number of Sequences: 518415
Number of Extensions: 167919208508
Number of Successful Extensions: 1034094832
Number of sequences better than 0.0: 0
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