BLASTX 7.6.2
Query= UN44766 /QuerySize=844
(843 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=... 267 1e-070
sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE... 265 3e-070
sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 P... 265 3e-070
sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 ... 264 6e-070
sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Ni... 264 8e-070
sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN... 264 8e-070
sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=... 263 2e-069
sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 ... 261 5e-069
sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 ... 259 2e-068
sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosacchar... 243 2e-063
sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoi... 240 1e-062
sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces ce... 218 6e-056
sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusio... 153 2e-036
sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusio... 145 5e-034
sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=N... 139 4e-032
sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1... 138 8e-032
sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 P... 137 1e-031
sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1... 136 2e-031
sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoi... 126 2e-028
sp|P55175|Y601_SYNY3 UPF0012 hydrolase sll0601 OS=Synechocystis ... 122 5e-027
>sp|Q6IR61|NIT2A_XENLA Omega-amidase NIT2-A OS=Xenopus laevis GN=nit2a PE=2
SV=1
Length = 276
Score = 267 bits (680), Expect = 1e-070
Identities = 126/204 (61%), Positives = 153/204 (75%)
Frame = -2
Query: 842 MLSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFM 663
+LS+V+K GI +IGGSIPE LYNTC VFG DG L KHRKIHLFDID+PGKI F
Sbjct: 68 LLSQVAKECGIYLIGGSIPEEDCGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQ 127
Query: 662 ESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
ES+TL+ G++ ++ DT ++G+GICYDIRF ELA IYA +G LL YPGAFNMTTGP H
Sbjct: 128 ESETLSPGDSFSVFDTPYCKVGVGICYDIRFAELAQIYANKGCQLLVYPGAFNMTTGPAH 187
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEID 303
WELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A EE ++ AEID
Sbjct: 188 WELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVVAKAGSEETVLSAEID 247
Query: 302 YSILEQRRTSLPLNKQRRGDLYQL 231
L + R +P+ +QRR DLY +
Sbjct: 248 LQYLAEIREQIPIRRQRRRDLYNV 271
>sp|Q2T9R6|NIT2_BOVIN Omega-amidase NIT2 OS=Bos taurus GN=NIT2 PE=2 SV=1
Length = 276
Score = 265 bits (676), Expect = 3e-070
Identities = 125/203 (61%), Positives = 152/203 (74%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LSEV+K + +IGGSIPE+ LYNTC VFG DG L KHRK+HLFDID+PGKITF E
Sbjct: 69 LSEVAKECSMYVIGGSIPEKDAGKLYNTCAVFGPDGTLLVKHRKLHLFDIDVPGKITFQE 128
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
S+TL+ G++ ++ DT R+G+GICYDIRF ELA IYA RG LL YPGAFN+TTGP HW
Sbjct: 129 SETLSPGDSFSLFDTPYCRVGLGICYDIRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHW 188
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EE I+ A+ID
Sbjct: 189 ELLQRGRAVDNQVYVATASPARDEKASYVAWGHSTVVNPWGEVLAKAGTEETIVYADIDL 248
Query: 299 SILEQRRTSLPLNKQRRGDLYQL 231
L + R +P+ Q+R DLY++
Sbjct: 249 KKLAEIRQQIPIFSQKRSDLYEV 271
>sp|Q4VBV9|NIT2_DANRE Omega-amidase NIT2 OS=Danio rerio GN=nit2 PE=2 SV=1
Length = 277
Score = 265 bits (676), Expect = 3e-070
Identities = 124/208 (59%), Positives = 154/208 (74%)
Frame = -2
Query: 842 MLSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFM 663
+LSE +K+ GI ++GGSIPE G LYNTC VFG DG L HRKIHLFDID+PGKI F
Sbjct: 68 VLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVFGPDGTLLVTHRKIHLFDIDVPGKIRFQ 127
Query: 662 ESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
ES+TL+ G++ ++ +T ++G+GICYDIRF ELA IYA +G LL YPGAFNMTTGP H
Sbjct: 128 ESETLSPGKSLSMFETPYCKVGVGICYDIRFAELAQIYAKKGCQLLVYPGAFNMTTGPAH 187
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEID 303
WELLQR RA DNQ+YVAT SPARD A Y AWGHS++I P+GEV++ EE+++ A+ID
Sbjct: 188 WELLQRGRAVDNQVYVATASPARDETASYVAWGHSSVINPWGEVISKAGSEESVVYADID 247
Query: 302 YSILEQRRTSLPLNKQRRGDLYQLVDVQ 219
L R +P+ KQRR DLY + VQ
Sbjct: 248 LQYLADVRQQIPITKQRRNDLYSVNSVQ 275
>sp|Q9NQR4|NIT2_HUMAN Omega-amidase NIT2 OS=Homo sapiens GN=NIT2 PE=1 SV=1
Length = 276
Score = 264 bits (674), Expect = 6e-070
Identities = 127/201 (63%), Positives = 152/201 (75%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LSEV+K I +IGGSIPE LYNTC VFG DG L AK+RKIHLFDID+PGKITF E
Sbjct: 69 LSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQE 128
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
SKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL YPGAFN+TTGP HW
Sbjct: 129 SKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHW 188
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELLQR+RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EEAI+ ++ID
Sbjct: 189 ELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDL 248
Query: 299 SILEQRRTSLPLNKQRRGDLY 237
L + R +P+ +Q+R DLY
Sbjct: 249 KKLAEIRQQIPVFRQKRSDLY 269
>sp|Q497B0|NIT2_RAT Omega-amidase NIT2 OS=Rattus norvegicus GN=Nit2 PE=1 SV=1
Length = 276
Score = 264 bits (673), Expect = 8e-070
Identities = 127/203 (62%), Positives = 150/203 (73%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LSEV+K I +IGGSIPE LYNTC VFG DG L KHRKIHLFDID+PGKITF E
Sbjct: 69 LSEVAKENSIYLIGGSIPEEDDGKLYNTCAVFGPDGNLLVKHRKIHLFDIDVPGKITFQE 128
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
SKTL+ G++ + DT R+G+GICYD+RF ELA IYA RG LL YPGAFNMTTGP HW
Sbjct: 129 SKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYARRGCQLLVYPGAFNMTTGPAHW 188
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G+VL EE I+ ++ID
Sbjct: 189 ELLQRARAVDNQVYVATASPARDEKASYVAWGHSTVVDPWGQVLTKAGTEETILYSDIDL 248
Query: 299 SILEQRRTSLPLNKQRRGDLYQL 231
L + R +P+ KQ+R DLY +
Sbjct: 249 KKLSEIRQQIPILKQKRADLYSV 271
>sp|Q28IE5|NIT2_XENTR Omega-amidase NIT2 OS=Xenopus tropicalis GN=nit2 PE=2
SV=1
Length = 276
Score = 264 bits (673), Expect = 8e-070
Identities = 123/203 (60%), Positives = 152/203 (74%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LS+V+K GI +IGGSIPE LYNTC VFG DG L KHRKIHLFDID+PGKI F E
Sbjct: 69 LSQVAKECGIYLIGGSIPEEDSGKLYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQE 128
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
S+TL+ G++ ++ +T ++G+GICYDIRF ELA +Y+ +G LL YPGAFNMTTGP HW
Sbjct: 129 SETLSPGDSFSVFETPYCKVGVGICYDIRFAELAQLYSKKGCQLLVYPGAFNMTTGPAHW 188
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A EE +I A+ID
Sbjct: 189 ELLQRARALDNQVYVATASPARDEKASYVAWGHSTIVSPWGEVIAKAGSEETVISADIDL 248
Query: 299 SILEQRRTSLPLNKQRRGDLYQL 231
L + R +P+ +QRR DLY +
Sbjct: 249 EYLAEIREQIPIRRQRRHDLYSV 271
>sp|Q6INI7|NIT2B_XENLA Omega-amidase NIT2-B OS=Xenopus laevis GN=nit2b PE=2
SV=1
Length = 276
Score = 263 bits (670), Expect = 2e-069
Identities = 123/202 (60%), Positives = 151/202 (74%)
Frame = -2
Query: 842 MLSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFM 663
+LS+V+K GI +IGGSIPE YNTC VFG DG L KHRKIHLFDID+PGKI F
Sbjct: 68 LLSQVAKECGIYLIGGSIPEEDSGKFYNTCAVFGPDGTLLVKHRKIHLFDIDVPGKIRFQ 127
Query: 662 ESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
ES+TL+ G++ ++ DT ++G+GICYD+RF ELA IYA +G LL YPGAFNMTTGP H
Sbjct: 128 ESETLSPGDSFSVFDTPYCKVGVGICYDMRFAELAQIYANKGCQLLVYPGAFNMTTGPAH 187
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEID 303
WELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+GEV+A EE +I A+ID
Sbjct: 188 WELLQRARALDNQVYVATASPARDEKASYVAWGHSTVVSPWGEVIAKAGFEETVISADID 247
Query: 302 YSILEQRRTSLPLNKQRRGDLY 237
L + R +P+ +QRR +LY
Sbjct: 248 LQYLAEIREQIPIRRQRRDNLY 269
>sp|Q9JHW2|NIT2_MOUSE Omega-amidase NIT2 OS=Mus musculus GN=Nit2 PE=1 SV=1
Length = 276
Score = 261 bits (666), Expect = 5e-069
Identities = 125/201 (62%), Positives = 149/201 (74%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LSEV+K I +IGGSIPE LYNTC VFG DG L KHRKIHLFDID+PGKITF E
Sbjct: 69 LSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDIDVPGKITFQE 128
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
SKTL+ G++ + DT ++G+GICYD+RF ELA IYA RG LL YPGAFN+TTGP HW
Sbjct: 129 SKTLSPGDSFSTFDTPYCKVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHW 188
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELLQRARA DNQ+YVAT SPARD A Y AWGHST++ P+G+VL EE I+ ++ID
Sbjct: 189 ELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAGTEETILYSDIDL 248
Query: 299 SILEQRRTSLPLNKQRRGDLY 237
L + R +P+ KQ+R DLY
Sbjct: 249 KKLAEIRQQIPILKQKRADLY 269
>sp|Q5R4L6|NIT2_PONAB Omega-amidase NIT2 OS=Pongo abelii GN=NIT2 PE=3 SV=1
Length = 275
Score = 259 bits (660), Expect = 2e-068
Identities = 127/201 (63%), Positives = 150/201 (74%), Gaps = 1/201 (0%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LSEV+K I +IGGSIPE LYNTC VFG DG L AK+RKIHLFDID+PGKITF E
Sbjct: 69 LSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQE 128
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
SKTL+ G++ DT R+G+GICYD+RF ELA IYA RG LL YPGAFN+TTGP HW
Sbjct: 129 SKTLSPGDSFCTFDT-YCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHW 187
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELLQR RA DNQ+YVAT SPARD A Y AWGHST++ P+GEVLA EEAI+ ++ID
Sbjct: 188 ELLQRGRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDL 247
Query: 299 SILEQRRTSLPLNKQRRGDLY 237
L + R +P+ +Q+R DLY
Sbjct: 248 KKLAEIRQQIPVFRQKRSDLY 268
>sp|Q10166|YAUB_SCHPO UPF0012 hydrolase C26A3.11 OS=Schizosaccharomyces pombe
GN=SPAC26A3.11 PE=2 SV=1
Length = 322
Score = 243 bits (618), Expect = 2e-063
Identities = 114/204 (55%), Positives = 150/204 (73%)
Frame = -2
Query: 839 LSEVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
LS ++K + GGSIPER LYNT VF G+L A HRKIHLFDIDIPG ++F E
Sbjct: 111 LSSMAKDTKTYLFGGSIPERKDGKLYNTAMVFDPSGKLIAVHRKIHLFDIDIPGGVSFRE 170
Query: 659 SKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHW 480
S +L+ G+ T+VDT+ G+ G+GICYDIRF ELAMI A G ++ YPGAFN++TGPLHW
Sbjct: 171 SDSLSPGDAMTMVDTEYGKFGLGICYDIRFPELAMIAARNGCSVMIYPGAFNLSTGPLHW 230
Query: 479 ELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDY 300
ELL RARA DN+++VA C+PARD A Y +WGHST++ PFG+V+ATT+ + +I+ A+ID
Sbjct: 231 ELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVDPFGKVIATTDEKPSIVYADIDP 290
Query: 299 SILEQRRTSLPLNKQRRGDLYQLV 228
S++ R S+P+ QRR D+Y V
Sbjct: 291 SVMSTARNSVPIYTQRRFDVYSEV 314
>sp|Q54JM9|NIT2_DICDI Nitrilase homolog 2 OS=Dictyostelium discoideum GN=nit2
PE=3 SV=1
Length = 328
Score = 240 bits (611), Expect = 1e-062
Identities = 112/209 (53%), Positives = 152/209 (72%), Gaps = 2/209 (0%)
Frame = -2
Query: 836 SEVSKRLGITIIGGSIPE--RVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFM 663
SE +KR I ++GGSIPE + +YNTC +F GE+ KHRKIHLFDID+P KI F
Sbjct: 119 SEAAKRNQIFLVGGSIPEIDKATGKIYNTCFIFNDKGEVVKKHRKIHLFDIDVPNKIRFK 178
Query: 662 ESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
ES+TLT G++ ++VD +IG+ ICYDIRF ELAM+Y+ GA L YPGAFNM TGP H
Sbjct: 179 ESETLTPGDSFSVVDIGYCKIGVAICYDIRFPELAMLYSKMGAKFLIYPGAFNMVTGPAH 238
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEID 303
WELLQR RA DNQ++VA SPAR+ + Y AWGHST++ +G +LATT+ ++II ++ID
Sbjct: 239 WELLQRGRAVDNQVFVAAISPARNPSSTYQAWGHSTIVNSWGTILATTDEHQSIIYSDID 298
Query: 302 YSILEQRRTSLPLNKQRRGDLYQLVDVQR 216
+ L + R+S+P+ Q+R DLY+L +++
Sbjct: 299 LNTLNETRSSIPIYSQKRDDLYKLDSIKK 327
>sp|P49954|NIT3_YEAST Probable hydrolase NIT3 OS=Saccharomyces cerevisiae
GN=NIT3 PE=1 SV=1
Length = 291
Score = 218 bits (553), Expect = 6e-056
Identities = 104/206 (50%), Positives = 142/206 (68%), Gaps = 2/206 (0%)
Frame = -2
Query: 836 SEVSKRLGITIIGGSIPE--RVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFM 663
S ++ + I ++GG+IPE D +YNT +F DG+L KHRK+HLFD+DIP I+F
Sbjct: 84 SNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLIDKHRKVHLFDVDIPNGISFH 143
Query: 662 ESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
ES+TL+ GE T +DT G+ G+GICYD+RF ELAM+ A +GA + YP AFN TGPLH
Sbjct: 144 ESETLSPGEKSTTIDTKYGKFGVGICYDMRFPELAMLSARKGAFAMIYPSAFNTVTGPLH 203
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEID 303
W LL R+RA DNQ+YV CSPAR+ + Y A+GHS ++ P G+++A E II AE+D
Sbjct: 204 WHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKIVAEAGEGEEIIYAELD 263
Query: 302 YSILEQRRTSLPLNKQRRGDLYQLVD 225
++E R ++PL KQRR D+Y V+
Sbjct: 264 PEVIESFRQAVPLTKQRRFDVYSDVN 289
>sp|O76463|NFT1_CAEEL Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Caenorhabditis elegans GN=nft-1 PE=1 SV=1
Length = 440
Score = 153 bits (385), Expect = 2e-036
Identities = 78/181 (43%), Positives = 108/181 (59%), Gaps = 1/181 (0%)
Frame = -2
Query: 770 HLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESKTLTAG-ETPTIVDTDVGRIGI 594
H +NT + SDG +A++ K+HLFD++IPGK+ MES+ AG E VDT +GR+G+
Sbjct: 107 HPWNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGL 166
Query: 593 GICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLHWELLQRARATDNQLYVATCSPAR 414
ICYD+RF EL++ RGA LL +P AF + TG HWE L RARA +NQ YV +
Sbjct: 167 SICYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTG 226
Query: 413 DSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAEIDYSILEQRRTSLPLNKQRRGDLYQ 234
++GHS ++ P+G V+A + AEID S ++ R P+ RR DLY
Sbjct: 227 AHNPKRQSYGHSMVVDPWGAVVAQCSERVDMCFAEIDLSYVDTLREMQPVFSHRRSDLYT 286
Query: 233 L 231
L
Sbjct: 287 L 287
>sp|O76464|NFT1_DROME Nitrilase and fragile histidine triad fusion protein
NitFhit OS=Drosophila melanogaster GN=NitFhit PE=1 SV=1
Length = 460
Score = 145 bits (364), Expect = 5e-034
Identities = 88/243 (36%), Positives = 128/243 (52%), Gaps = 7/243 (2%)
Frame = -2
Query: 833 EVSKRLGITIIGGSIPERVGDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMESK 654
E++K I I G + ER ++N + GEL A +RK+H+FD+ ++ ES
Sbjct: 101 ELAKCNKIWISLGGVHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTK-EVRLRESD 159
Query: 653 TLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
T+T G E P V T VG+IG+ ICYD+RF E A++ GA+LL YP AF TG H
Sbjct: 160 TVTPGYCLERP--VSTPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYATGKAH 217
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLA-TTEHEEAIIIAEI 306
WE+L RARA + Q +V + +WGHS ++ P+G VLA +E E I AE+
Sbjct: 218 WEILLRARAIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIGTAEV 277
Query: 305 DYSILEQRRTSLPLNKQRRGDLYQLVDVQRLNSK*VQRERECVHLEMLSLAFVESENCLV 126
D S+L+ ++P + RR D+Y L + + Q ++ F ESE+C
Sbjct: 278 DLSVLQSLYQTMPCFEHRRNDIYALTAYNLRSKEPTQDRPFATNIVDKRTIFYESEHCFA 337
Query: 125 TCN 117
N
Sbjct: 338 FTN 340
>sp|Q7TQ94|NIT1_RAT Nitrilase homolog 1 OS=Rattus norvegicus GN=Nit1 PE=2 SV=1
Length = 292
Score = 139 bits (348), Expect = 4e-032
Identities = 82/207 (39%), Positives = 113/207 (54%), Gaps = 16/207 (7%)
Frame = -2
Query: 824 KRLGITIIGGSIPERVGD-----HLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
+ GI + G ER D +YN + S G + A +RK HL D++IPG+ E
Sbjct: 83 RECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRE 142
Query: 659 SKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGP 489
S G E P V T G++G+ ICYD+RF EL++ A GA +L YP AF TGP
Sbjct: 143 SNYTMPGYALEPP--VKTPAGKVGLAICYDMRFPELSLKLAQAGAEILTYPSAFGSVTGP 200
Query: 488 LHWELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAII 318
HWE+L RARA ++Q YV A C ++ A Y GHS ++ P+G V+A+ +
Sbjct: 201 AHWEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPWGTVVASCSEGPGLC 257
Query: 317 IAEIDYSILEQRRTSLPLNKQRRGDLY 237
+A ID L+Q R LP+ + RR DLY
Sbjct: 258 LARIDLHFLQQMRQHLPVFQHRRPDLY 284
>sp|Q86X76|NIT1_HUMAN Nitrilase homolog 1 OS=Homo sapiens GN=NIT1 PE=1 SV=2
Length = 327
Score = 138 bits (345), Expect = 8e-032
Identities = 77/204 (37%), Positives = 110/204 (53%), Gaps = 10/204 (4%)
Frame = -2
Query: 824 KRLGITIIGGSIPERVGD-----HLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
+ G+ + G ER D +YN + S G + A +RK HL D++IPG+ E
Sbjct: 118 RECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCE 177
Query: 659 SKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGP 489
S + G E+P V T G+IG+ +CYD+RF EL++ A GA +L YP AF TGP
Sbjct: 178 SNSTMPGPSLESP--VSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGP 235
Query: 488 LHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAE 309
HWE+L RARA + Q YV + ++GHS ++ P+G V+A + +A
Sbjct: 236 AHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLAR 295
Query: 308 IDYSILEQRRTSLPLNKQRRGDLY 237
ID + L Q R LP+ + RR DLY
Sbjct: 296 IDLNYLRQLRRHLPVFQHRRPDLY 319
>sp|Q32LH4|NIT1_BOVIN Nitrilase homolog 1 OS=Bos taurus GN=NIT1 PE=2 SV=1
Length = 328
Score = 137 bits (343), Expect = 1e-031
Identities = 75/204 (36%), Positives = 110/204 (53%), Gaps = 10/204 (4%)
Frame = -2
Query: 824 KRLGITIIGGSIPERVGD-----HLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
+ G+ + G ER D +YN + + G + A +RK HL D++IPG+ E
Sbjct: 119 RECGLWLSLGGFHERGQDWEQTQKIYNCHVIMNNMGSVVATYRKTHLCDVEIPGQGPMRE 178
Query: 659 SKTLTAG---ETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGP 489
S + G E+P + T G+IG+ ICYD+RF EL++ GA +L YP AF TGP
Sbjct: 179 SNSTIPGPSLESP--ISTPAGKIGLAICYDMRFPELSLALVQAGAEILTYPSAFGSVTGP 236
Query: 488 LHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIAE 309
HWE+L RARA + Q YV + ++GHS ++ P+G V+A + +A
Sbjct: 237 AHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLAR 296
Query: 308 IDYSILEQRRTSLPLNKQRRGDLY 237
ID + L+Q R LP+ + RR DLY
Sbjct: 297 IDLNYLQQLRKQLPVFQHRRPDLY 320
>sp|Q8VDK1|NIT1_MOUSE Nitrilase homolog 1 OS=Mus musculus GN=Nit1 PE=2 SV=1
Length = 323
Score = 136 bits (342), Expect = 2e-031
Identities = 80/205 (39%), Positives = 110/205 (53%), Gaps = 12/205 (5%)
Frame = -2
Query: 824 KRLGITIIGGSIPERVGD-----HLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFME 660
+ GI + G ER D +YN + S G + A +RK HL D++IPG+ E
Sbjct: 114 RECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVEIPGQGPMRE 173
Query: 659 SK-TLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
S T G V T G++G+ ICYD+RF EL++ A GA +L Y AF TGP H
Sbjct: 174 SNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGAEILTYSSAFGSVTGPAH 233
Query: 482 WELLQRARATDNQLYV---ATCSPARDSGAGYTAWGHSTLIGPFGEVLATTEHEEAIIIA 312
WE+L RARA ++Q YV A C ++ A Y GHS ++ P+G V+A + +A
Sbjct: 234 WEVLLRARAIESQCYVIAAAQCGRHHETRASY---GHSMVVDPWGTVVARCSEGPGLCLA 290
Query: 311 EIDYSILEQRRTSLPLNKQRRGDLY 237
ID L+Q R LP+ + RR DLY
Sbjct: 291 RIDLHFLQQMRQHLPVFQHRRPDLY 315
>sp|Q557J5|NIT1_DICDI Nitrilase homolog 1 OS=Dictyostelium discoideum GN=nit1-1
PE=3 SV=1
Length = 291
Score = 126 bits (315), Expect = 2e-028
Identities = 68/205 (33%), Positives = 115/205 (56%), Gaps = 6/205 (2%)
Frame = -2
Query: 827 SKRLGITIIGGSIPERV----GDHLYNTCCVFGSDGELKAKHRKIHLFDIDIPGK-ITFM 663
+K+ I + G E++ D +YNT + S+G + ++RK+HLFD+DIP K +
Sbjct: 87 AKQNNIWLSLGGFHEKILDDPNDMIYNTHLIIDSNGVIVCEYRKMHLFDVDIPSKGVKMN 146
Query: 662 ESKTLTAGETPTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
ESK + G + D+ VG++G+ ICYD+RF EL + A +L P AF +TG H
Sbjct: 147 ESKVVKGGNDLVVCDSPVGKLGLSICYDLRFPELYLSLRRMDAQILLVPSAFMKSTGEAH 206
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLIGPFGEVL-ATTEHEEAIIIAEI 306
W+ L +ARA +NQ YV + D + +++GHS +I P+G+VL ++ I +I
Sbjct: 207 WKPLLQARAIENQTYVIAAAQTGDHHSKRSSYGHSMIIDPWGKVLHDLPDNLNDIAFVDI 266
Query: 305 DYSILEQRRTSLPLNKQRRGDLYQL 231
D + R ++P+ ++ + Y++
Sbjct: 267 DLDYISTCRENIPVFNHKKLNNYKI 291
>sp|P55175|Y601_SYNY3 UPF0012 hydrolase sll0601 OS=Synechocystis sp. (strain PCC
6803) GN=sll0601 PE=3 SV=1
Length = 272
Score = 122 bits (304), Expect = 5e-027
Identities = 72/200 (36%), Positives = 113/200 (56%), Gaps = 11/200 (5%)
Frame = -2
Query: 827 SKRLGITIIGGSIPERVGDH---LYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMES 657
++R +TI+ G P V YNT + +G+ A++ K+HLFD+++P T+ ES
Sbjct: 73 AQRFQVTILAGGFPFPVAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWES 132
Query: 656 KTLTAGET-PTIVDTD-VGRIGIGICYDIRFQELAMIYAARGAHLLCYPGAFNMTTGPLH 483
T+ AG+ P + +D G +G+ ICYD+RF EL + +GA +L P AF TG H
Sbjct: 133 ATVMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDH 192
Query: 482 WELLQRARATDNQLYVATCSPARDSGAGYT---AWGHSTLIGPFGEVLATTEHEEAIIIA 312
W++L +ARA +N YV +PA+ +G Y GH+ +I P+G +LA + + IA
Sbjct: 193 WQVLLQARAIENTCYV--IAPAQ-TGCHYERRHTHGHAMIIDPWGVILADAGEKPGLAIA 249
Query: 311 EIDYSILEQRRTSLPLNKQR 252
EI+ L+Q R +P + R
Sbjct: 250 EINPDRLKQVRQQMPSLQHR 269
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 171,432,990,471
Number of Sequences: 518415
Number of Extensions: 171432990471
Number of Successful Extensions: 1050360658
Number of sequences better than 0.0: 0
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