BLASTX 7.6.2
Query= UN47226 /QuerySize=981
(980 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q28891|S5A1_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 79 6e-014
sp|P18405|S5A1_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 78 1e-013
sp|Q28892|S5A2_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=... 76 4e-013
sp|Q68FF9|S5A1_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 75 8e-013
sp|P24008|S5A1_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Ra... 75 8e-013
sp|P31213|S5A2_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=... 74 1e-012
sp|A5PJS2|S5A1_BOVIN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=... 73 2e-012
sp|Q99N99|S5A2_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=... 71 9e-012
sp|O18765|S5A2_PIG 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Su... 70 2e-011
sp|P31214|S5A2_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Ra... 70 2e-011
sp|P40526|DFG10_YEAST Protein DFG10 OS=Saccharomyces cerevisiae ... 61 9e-009
sp|Q8AVI9|S5A3_XENLA 3-oxo-5-alpha-steroid 4-dehydrogenase 3 OS=... 55 9e-007
sp|Q0P4J9|S5A3_XENTR 3-oxo-5-alpha-steroid 4-dehydrogenase 3 OS=... 55 9e-007
>sp|Q28891|S5A1_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca
fascicularis GN=SRD5A1 PE=1 SV=1
Length = 263
Score = 79 bits (192), Expect = 6e-014
Identities = 41/118 (34%), Positives = 70/118 (59%), Gaps = 4/118 (3%)
Frame = +1
Query: 496 LTEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLF 675
+T+P F +G +++ G++ N++ +L LR + G YKIP+GGLF+ + +Y
Sbjct: 150 VTDPRF---LIGFGLWLAGMLINIHSDHILRNLR-KPGDTGYKIPRGGLFEYVTAANYFG 205
Query: 676 EILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
EI+ + + + S ++ +FA T +L GR+ WYL KF+++PK K LIPF+F
Sbjct: 206 EIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263
>sp|P18405|S5A1_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens
GN=SRD5A1 PE=1 SV=1
Length = 259
Score = 78 bits (190), Expect = 1e-013
Identities = 40/118 (33%), Positives = 70/118 (59%), Gaps = 4/118 (3%)
Frame = +1
Query: 496 LTEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLF 675
+T+P F +G +++ G++ N++ +L LR + G YKIP+GGLF+ + +Y
Sbjct: 146 VTDPRF---LIGFGLWLTGMLINIHSDHILRNLR-KPGDTGYKIPRGGLFEYVTAANYFG 201
Query: 676 EILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
EI+ + + + S ++ +FA T +L GR+ WYL KF+++PK K +IPF+F
Sbjct: 202 EIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259
>sp|Q28892|S5A2_MACFA 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca
fascicularis GN=SRD5A2 PE=1 SV=1
Length = 254
Score = 76 bits (185), Expect = 4e-013
Identities = 49/177 (27%), Positives = 92/177 (51%), Gaps = 8/177 (4%)
Frame = +1
Query: 331 LLKSALALHFFKRIFEVLFIHK---YSGEMAIDSALTITSSYFSSTALLLYSQNFTLG-L 498
+L +H+F R F +++ Y + + F + L+Y + G
Sbjct: 82 VLLGLFCVHYFHRTFVYSLLNRGRPYPAVLIFRGIAFCAGNGFLQSYYLIYCAEYPDGWY 141
Query: 499 TEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLFE 678
T+ F LG+ +F++G+ N++ +L +LR + G Y+IPKGGLF + ++L E
Sbjct: 142 TDIRF---CLGVFLFILGMGVNIHSDYILRQLR-KPGEITYRIPKGGLFTYVSGANFLGE 197
Query: 679 ILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
I+ + + + + ++ + +FA + +L R++ +YL F+D+PK KALIPF+F
Sbjct: 198 IIEWIGYALATWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254
>sp|Q68FF9|S5A1_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus
GN=Srd5a1 PE=2 SV=2
Length = 255
Score = 75 bits (182), Expect = 8e-013
Identities = 42/117 (35%), Positives = 66/117 (56%), Gaps = 4/117 (3%)
Frame = +1
Query: 496 LTEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLF 675
+T P F G +++VG+V N++ +L LR + G YKIP+GGLF+ + +Y
Sbjct: 142 VTHPCF---LTGFALWLVGMVINIHSDHILRNLR-KPGETGYKIPRGGLFEYVSSANYFG 197
Query: 676 EILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFV 846
E++ + F + S ++ FA+ T L R+ WYL KF+D+PK K LIPF+
Sbjct: 198 ELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254
>sp|P24008|S5A1_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus
GN=Srd5a1 PE=1 SV=2
Length = 259
Score = 75 bits (182), Expect = 8e-013
Identities = 42/117 (35%), Positives = 66/117 (56%), Gaps = 4/117 (3%)
Frame = +1
Query: 496 LTEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLF 675
+T P F G +++VG+V N++ +L LR + G YKIP+GGLF+ + +Y
Sbjct: 146 VTHPCF---LTGFALWLVGMVINIHSDHILRNLR-KPGETGYKIPRGGLFEYVSAANYFG 201
Query: 676 EILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFV 846
E++ + F + S ++ FA+ T L+ R+ WY KF+D+PK K LIPFV
Sbjct: 202 ELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258
>sp|P31213|S5A2_HUMAN 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens
GN=SRD5A2 PE=1 SV=1
Length = 254
Score = 74 bits (180), Expect = 1e-012
Identities = 48/177 (27%), Positives = 92/177 (51%), Gaps = 8/177 (4%)
Frame = +1
Query: 331 LLKSALALHFFKRIFEVLFIHK---YSGEMAIDSALTITSSYFSSTALLLYSQNFTLG-L 498
+L +H+F R F +++ Y + + T + L+Y + G
Sbjct: 82 VLLGLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGWY 141
Query: 499 TEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLFE 678
T+ F LG+ +F++G+ N++ +L +LR + G Y+IP+GGLF + ++L E
Sbjct: 142 TDIRFS---LGVFLFILGMGINIHSDYILRQLR-KPGEISYRIPQGGLFTYVSGANFLGE 197
Query: 679 ILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
I+ + + + + ++ + +FA + +L R++ +YL F+D+PK KALIPF+F
Sbjct: 198 IIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254
>sp|A5PJS2|S5A1_BOVIN 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus
GN=SRD5A1 PE=2 SV=1
Length = 257
Score = 73 bits (178), Expect = 2e-012
Identities = 43/136 (31%), Positives = 74/136 (54%), Gaps = 4/136 (2%)
Frame = +1
Query: 439 SSYFSSTALLLYSQNFTLGLTEPAFDLKPLGIVMFVVGIVGNLYHHVLLAKLRSEDGTKE 618
+ Y S L Y+ L++P F G ++++G++ N++ +L LR + G
Sbjct: 125 NGYLQSRYLSQYAVYADDWLSDPRF---LTGSALWLIGMLINIHSDHVLRNLR-KPGETG 180
Query: 619 YKIPKGGLFDTIICPHYLFEILVFWSFFMVSQTIYSFSFAMGTTFYLVGRSYATRSWYLS 798
YKIP+GGLF+ I +Y E++ + + + S +I ++FA+ T L R+ WY
Sbjct: 181 YKIPRGGLFEYISAANYFGEVVEWCGYALASWSIQGWAFAVFTFCVLFTRAQQHHKWYHE 240
Query: 799 KFDDFPKHIKALIPFV 846
KF+D+PK K +IPF+
Sbjct: 241 KFEDYPKFRKIMIPFL 256
>sp|Q99N99|S5A2_MOUSE 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Mus musculus
GN=Srd5a2 PE=2 SV=1
Length = 254
Score = 71 bits (173), Expect = 9e-012
Identities = 36/108 (33%), Positives = 67/108 (62%), Gaps = 1/108 (0%)
Frame = +1
Query: 526 LGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLFEILVFWSFFM 705
+G+ F++G+ N++ +L +LR + G Y+IP+GGLF + ++L EI+ + + +
Sbjct: 148 VGVFFFILGMGINIHSDCMLRQLR-KPGEVIYRIPQGGLFTYVSGANFLGEIIEWMGYAL 206
Query: 706 VSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
+ ++ +F+FA T +L +++ +YL F D+PK KALIPF+F
Sbjct: 207 ATWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF 254
>sp|O18765|S5A2_PIG 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa
GN=SRD5A2 PE=2 SV=2
Length = 254
Score = 70 bits (171), Expect = 2e-011
Identities = 36/108 (33%), Positives = 68/108 (62%), Gaps = 1/108 (0%)
Frame = +1
Query: 526 LGIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLFEILVFWSFFM 705
LG+ +F++G+ N++ +L +LR + G YKIP+GGLF + ++L EI+ + + +
Sbjct: 148 LGVFLFILGMGINIHSDYILRQLR-KPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206
Query: 706 VSQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
+ ++ + +FA + +L R++ +Y+ F+D+PK KALIPF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254
>sp|P31214|S5A2_RAT 3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Rattus norvegicus
GN=Srd5a2 PE=1 SV=1
Length = 254
Score = 70 bits (170), Expect = 2e-011
Identities = 36/107 (33%), Positives = 66/107 (61%), Gaps = 1/107 (0%)
Frame = +1
Query: 529 GIVMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLFEILVFWSFFMV 708
G+ +F++G+ N++ L +LR + G Y+IP+GGLF + ++L EI+ + + +
Sbjct: 149 GVFLFILGMGINIHSDYTLRQLR-KPGEVIYRIPRGGLFTYVSGANFLGEIIEWIGYALA 207
Query: 709 SQTIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFVF 849
+ ++ +F+FA T +L +++ +YL F D+PK KALIPF+F
Sbjct: 208 TWSVPAFAFAFFTLCFLGMQAFYHHRFYLKMFKDYPKSRKALIPFIF 254
>sp|P40526|DFG10_YEAST Protein DFG10 OS=Saccharomyces cerevisiae GN=DFG10 PE=2
SV=1
Length = 253
Score = 61 bits (147), Expect = 9e-009
Identities = 43/164 (26%), Positives = 76/164 (46%), Gaps = 10/164 (6%)
Frame = +1
Query: 355 HFFKRIFEVLFIHKYSGEMAIDSALTITSSYFSSTALLLYSQNFTLGLTEPAFDLKPLGI 534
H +R++E L++ Y+ ++ + + +F S LL+ N +L L
Sbjct: 99 HSLRRLYETLYVLHYTSNSRMNWSHYLVGIWFYSVLLLIL--NISLYKNSIPNTLNMNAF 156
Query: 535 VMFVVGIVGNLYHHVLLAKLRSEDGTKEYKIPKGGLFDTIICPHYLFEILVFWSFFMVSQ 714
++F + +HV+LA L +Y +P G LF + CPHYL EI+++ + Q
Sbjct: 157 IIFCIASWDQYKNHVILANL------VKYSLPTGRLFRLVCCPHYLDEIIIYSTLLPYEQ 210
Query: 715 TIYSFSFAMGTTFYLVGRSYATRSWYLSKFDDFPKHIKALIPFV 846
Y + T+ L + T+++Y KF D A+IPF+
Sbjct: 211 EFYLTLVWVITS--LTISALETKNYYRHKFKDNHVAPYAIIPFI 252
>sp|Q8AVI9|S5A3_XENLA 3-oxo-5-alpha-steroid 4-dehydrogenase 3 OS=Xenopus laevis
GN=srd5a3 PE=2 SV=1
Length = 319
Score = 55 bits (130), Expect = 9e-007
Identities = 32/115 (27%), Positives = 57/115 (49%), Gaps = 8/115 (6%)
Frame = +1
Query: 526 LGIVMFVVGIVGNLYHHVLLAKLRSEDG----TKEYKIPKGGLFDTIICPHYLFEILVFW 693
LG+ ++V + H +LA LR ++ +P G F+ + CPHY E+L++
Sbjct: 206 LGLTLYVWASLHQYTCHCILADLRKSASGAIINLKHAVPTGDWFEKVSCPHYFAELLIYL 265
Query: 694 SFFMVSQTIYSFSFAMGTTFYLVGRSYAT---RSWYLSKFDDFPKHIKALIPFVF 849
S +V + + + + + L+ ++ A +Y KFD +P H KA IP +F
Sbjct: 266 SIAVVFGLLNTIWWLV-VLYVLLSQALAAVLCHEFYHEKFDSYPIHRKAFIPLIF 319
>sp|Q0P4J9|S5A3_XENTR 3-oxo-5-alpha-steroid 4-dehydrogenase 3 OS=Xenopus
tropicalis GN=srd5a3 PE=2 SV=1
Length = 308
Score = 55 bits (130), Expect = 9e-007
Identities = 32/115 (27%), Positives = 57/115 (49%), Gaps = 8/115 (6%)
Frame = +1
Query: 526 LGIVMFVVGIVGNLYHHVLLAKLRSEDG----TKEYKIPKGGLFDTIICPHYLFEILVFW 693
LG+ +++ + H +LA LR + +P G F+ + CPHY E+L++
Sbjct: 195 LGLALYIWASLHQYRCHCILAGLRKSASGNVINLNHSVPCGDWFERVSCPHYFAELLIYV 254
Query: 694 SFFMVSQTIYSFSFAMGTTFYLVGRSYAT---RSWYLSKFDDFPKHIKALIPFVF 849
S +V + + + + + L+ ++ A +Y KFD +P H KA IPF+F
Sbjct: 255 SIAVVFGLLNTIWWLV-VLYVLLNQALAALLCHEFYHEKFDTYPIHRKAFIPFIF 308
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 177,416,216,708
Number of Sequences: 518415
Number of Extensions: 177416216708
Number of Successful Extensions: 1076203161
Number of sequences better than 0.0: 0
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