BLASTX 7.6.2
Query= UN49716 /QuerySize=1073
(1072 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 230 2e-059
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 222 4e-057
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 196 2e-049
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 196 3e-049
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 196 3e-049
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 196 4e-049
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 194 1e-048
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 194 1e-048
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 190 2e-047
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 189 3e-047
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 188 6e-047
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 187 1e-046
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 175 5e-043
sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=... 128 9e-029
sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sap... 123 3e-027
sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1,... 122 7e-027
sp|Q5PRC0|CH25H_DANRE Cholesterol 25-hydroxylase-like protein OS... 115 8e-025
sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS... 112 7e-024
sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS... 109 6e-023
sp|O95992|CH25H_HUMAN Cholesterol 25-hydroxylase OS=Homo sapiens... 96 5e-019
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 230 bits (584), Expect = 2e-059
Identities = 111/249 (44%), Positives = 152/249 (61%), Gaps = 4/249 (1%)
Frame = +1
Query: 121 VTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQ-AKNNTPAVQEKCITR 297
V ++ + L G+F+ HE +F S +PF + FL KYKIQ K N Q CI +
Sbjct: 17 VDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKKNEWKTQFNCIFK 76
Query: 298 LLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILH 477
+L+ H V LP+M P +A+G+ + PLPS + I F+IEDF FYW HR LH
Sbjct: 77 VLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYFYWVHRALH 136
Query: 478 TKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRV 657
+ YK +H VHH++A PFG+T+EYAHP E + LG T++GP L L TLW+W+ R+
Sbjct: 137 HGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTLWVWLGTRL 196
Query: 658 LETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGY 837
+TVE H GY FPW+ + +P +GGS FHD+HH T GNYSSTF Y+D IFGT Y
Sbjct: 197 FQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHE---TFVGNYSSTFTYLDKIFGTSDKY 253
Query: 838 RRLKSLKEN 864
K ++++
Sbjct: 254 YSRKQIRDS 262
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 222 bits (565), Expect = 4e-057
Identities = 104/236 (44%), Positives = 146/236 (61%), Gaps = 4/236 (1%)
Frame = +1
Query: 121 VTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKI-QAKNNTPAVQEKCITR 297
V ++ + L G+F+ H+ +F +PF+ + F KYKI Q K N Q C +
Sbjct: 17 VDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTKENDWKSQTYCAIK 76
Query: 298 LLLYHFCVNLPLMMASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILH 477
++L + LP+M P +A+G+ + PLPS + ++ FIIEDF FYW HR LH
Sbjct: 77 VILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIEDFYFYWAHRALH 136
Query: 478 TKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRV 657
YK +H VHH+YA+PFG+T+EYAHP E + LG T++GP L L TLW+W+ +R+
Sbjct: 137 HGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRDLFTLWVWLGVRL 196
Query: 658 LETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 825
+TVE H GY FPWS +N +P +GG+ FHDYHH + GNY+STF Y+D IFGT
Sbjct: 197 YQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEVFI---GNYASTFTYLDKIFGT 249
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 196 bits (498), Expect = 2e-049
Identities = 93/237 (39%), Positives = 142/237 (59%), Gaps = 9/237 (3%)
Frame = +1
Query: 160 SFLLHESVFFLSGLPFIYLERQGFLTKYKIQAKN-NTPAVQEKCITRLLLYHFCVNLPLM 336
SFLLHE ++F +P++ ++ + ++KIQ K T A Q +C +LL HF V LP +
Sbjct: 52 SFLLHELIYFGRCIPWMIIDAMPYFRRWKIQPKKVPTLAEQWECTRLVLLSHFTVELPQI 111
Query: 337 MASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHH 516
P+ G+ +S P P ++ QI +F +ED YW HR+ H Y+ +H VHH
Sbjct: 112 WLFDPMCATFGLSTSVPFPPVTKMIWQITLFFFLEDTWHYWAHRLFHYGIFYRFIHKVHH 171
Query: 517 EYATPFGLTSEYAHPAEILFLGFATIVGPAL-----TGPHLITLWLWMVLRVLETVEAHC 681
Y+ PFGL++EYAHP EI+ LG T+ P + HL+T+++W+ LR+ + V++H
Sbjct: 172 RYSAPFGLSAEYAHPLEIILLGAGTVFVPLMWCYFTHDLHLVTMYIWITLRLFQAVDSHA 231
Query: 682 GYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKS 852
GY FPWSL+ FLP++ G+D HDYHH N+SS+F + D + TD+ Y + K+
Sbjct: 232 GYDFPWSLNKFLPIWAGADHHDYHHMAF---KDNFSSSFRWWDAVLKTDQNYHQFKA 285
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 196 bits (497), Expect = 3e-049
Identities = 99/243 (40%), Positives = 141/243 (58%), Gaps = 5/243 (2%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS ++HE+++FL LP + ++ KYKIQ K T Q KC
Sbjct: 39 YMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCF 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVFYWGHR 468
LL HFC+ LPL+ +Y + + +P W + A+ +IED Y+ HR
Sbjct: 99 KVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I LW W+
Sbjct: 159 LLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
+R+LET++ H GY P + N +P Y GS HD+HH GNY+STF + D IFGTD
Sbjct: 219 IRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDRIFGTD 275
Query: 829 KGY 837
Y
Sbjct: 276 SQY 278
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 196 bits (497), Expect = 3e-049
Identities = 99/243 (40%), Positives = 141/243 (58%), Gaps = 5/243 (2%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS ++HE+++FL LP + ++ KYKIQ K T Q KC
Sbjct: 39 YMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCF 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVFYWGHR 468
LL HFC+ LPL+ +Y + + +P W + A+ +IED Y+ HR
Sbjct: 99 KVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I LW W+
Sbjct: 159 LLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
+R+LET++ H GY P + N +P Y GS HD+HH GNY+STF + D IFGTD
Sbjct: 219 IRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDRIFGTD 275
Query: 829 KGY 837
Y
Sbjct: 276 SQY 278
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 196 bits (496), Expect = 4e-049
Identities = 101/258 (39%), Positives = 149/258 (57%), Gaps = 9/258 (3%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS ++HE+++FL LP + F+ KYKIQ K T Q KC+
Sbjct: 39 YMLDNYTKFQIATWGSLIVHETIYFLFSLPGFLFQFIPFMRKYKIQKDKPETFEGQWKCL 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVFYWGHR 468
+L HF + LPL+ +Y + + +P W A+ L +IED Y+ HR
Sbjct: 99 KGILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYFTLARCLGCAVIEDTWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I LW W+
Sbjct: 159 LLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
+R+LET++ H GY P + N++P Y G+ HD+HH GNY+STF + D IFGTD
Sbjct: 219 MRLLETIDVHSGYDIPLNPLNYIPFYTGARHHDFHHMNFI---GNYASTFTWWDRIFGTD 275
Query: 829 KGY----RRLKSLKENSD 870
Y ++K L + S+
Sbjct: 276 VQYHAYTEKMKKLGKKSE 293
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 194 bits (492), Expect = 1e-048
Identities = 98/243 (40%), Positives = 140/243 (57%), Gaps = 5/243 (2%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS ++HE+++F LP + ++ KYKIQ K T Q KC
Sbjct: 39 YMLNNYTKFQIATWGSLIVHEALYFSFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCF 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVFYWGHR 468
LL HFC+ LPL+ +Y + + +P W + A+ +IED Y+ HR
Sbjct: 99 KVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFMHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I LW W+
Sbjct: 159 LLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
+R+LET++ H GY P + N +P Y GS HD+HH GNY+STF + D IFGTD
Sbjct: 219 IRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDRIFGTD 275
Query: 829 KGY 837
Y
Sbjct: 276 SQY 278
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 194 bits (492), Expect = 1e-048
Identities = 100/258 (38%), Positives = 148/258 (57%), Gaps = 9/258 (3%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS ++HE+++FL LP + ++ KYKIQ K T Q KC+
Sbjct: 39 YMLDNYTKFQIATWGSLIVHEAIYFLFSLPGFLFQFIPYMRKYKIQKDKPETFEGQWKCL 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSFP-LPSWKEVSAQILFYFIIEDFVFYWGHR 468
++L HF + LPL+ +Y + + +P W A+ L +IED Y+ HR
Sbjct: 99 KKILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYLTLARCLGCAVIEDTWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H VHHE+ PFG+ +EYAHP E L LG +G L H+I LW W+
Sbjct: 159 LLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
+R+LET++ H GY P + N +P Y G+ HD+HH GNY+STF + D +FGTD
Sbjct: 219 IRLLETIDVHSGYDIPLNPLNLVPFYTGARHHDFHHMNFI---GNYASTFTWWDKLFGTD 275
Query: 829 KGY----RRLKSLKENSD 870
Y + K L + SD
Sbjct: 276 AQYHAYIEKSKKLGKKSD 293
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 190 bits (481), Expect = 2e-047
Identities = 95/256 (37%), Positives = 146/256 (57%), Gaps = 5/256 (1%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS L+HE+ +FL +P + ++ KYKIQ K T Q KC
Sbjct: 39 YMLDNYTKFQIATWGSLLVHEASYFLLCVPGFIFQFIPYMQKYKIQPDKPETWEKQWKCF 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSF-PLPSWKEVSAQILFYFIIEDFVFYWGHR 468
L+ HF + LPL+ +Y + + +P W + AQ +IED Y+ HR
Sbjct: 99 KTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWYVLLAQCFGCAVIEDAWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H VHHE+ +PFG+ +EYAHP E L LG +G + H++ LW W++
Sbjct: 159 LLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILGAGFFIGIVVFCNHVVLLWAWVI 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
R++ET++ H GY P + + +P Y G+ FHD+HH GNY+STF + D IFGTD
Sbjct: 219 CRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHHMNFI---GNYASTFTWWDRIFGTD 275
Query: 829 KGYRRLKSLKENSDLK 876
+ K ++ L+
Sbjct: 276 SQFIAYKEKEKKQQLR 291
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 189 bits (479), Expect = 3e-047
Identities = 94/254 (37%), Positives = 142/254 (55%), Gaps = 5/254 (1%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
Y++ +++ FQ+A GS ++HE+++F LP + ++ KYKIQ K T Q KC
Sbjct: 39 YMLNNYTKFQIATWGSLIVHEALYFFFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCF 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSF-PLPSWKEVSAQILFYFIIEDFVFYWGHR 468
LL HFC+ PL+ +Y + + +P W A+ +IED Y+ HR
Sbjct: 99 KVLLFNHFCIQFPLICGTYYFTEYFSIPYDWETMPRWYIALARCFGCAVIEDTWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
+LH K +YK +H +HHE+ PFG+ +EYAHP E L LG +G L H+I LW W+
Sbjct: 159 LLHHKRIYKYIHKIHHEFQAPFGMEAEYAHPLETLILGTGFFIGIMLLCDHVILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
+R++ET++ H GY P + + +P Y GS HD+HH GNY+STF + D IFGTD
Sbjct: 219 VRLIETIDVHSGYDIPLNPLHLIPFYAGSRHHDFHHMNFI---GNYASTFTWWDRIFGTD 275
Query: 829 KGYRRLKSLKENSD 870
Y ++ ++
Sbjct: 276 AQYNAYNEKRKKAE 289
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 188 bits (477), Expect = 6e-047
Identities = 93/245 (37%), Positives = 139/245 (56%), Gaps = 5/245 (2%)
Frame = +1
Query: 115 YLVTHFSDFQLACIGSFLLHESVFFLSGLPFIYLERQGFLTKYKIQA-KNNTPAVQEKCI 291
+++ +++ FQ+A GS ++HE ++FL LP + F+ KYKIQ K T Q KC
Sbjct: 39 HMLQNYTKFQIATWGSLIVHELIYFLFCLPGFIFQFLPFMQKYKIQPDKPETWEKQWKCF 98
Query: 292 TRLLLYHFCVNLPLMMASYPVFRAMGMRSSF-PLPSWKEVSAQILFYFIIEDFVFYWGHR 468
LL HFC+ LPL+ +Y + + +P W + AQ +IED Y+ HR
Sbjct: 99 KMLLFNHFCIQLPLICGTYYFTEFFSIPYDWDTMPRWPFLLAQCFGCAVIEDTWHYFLHR 158
Query: 469 ILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMV 648
LH + +YK +H VHH++ +PFG+ +EYAHP E L LG +G + H+I LW W+
Sbjct: 159 ALHHRRIYKYIHKVHHDFTSPFGMQAEYAHPLETLILGAGFFIGTMVFCNHMILLWAWVT 218
Query: 649 LRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTD 828
R+LET++ H GY P + + +P Y G+ FHD+HH GNY STF + D +F TD
Sbjct: 219 FRLLETIDVHSGYDIPLNPLHLIPFYAGARFHDFHHMNFV---GNYGSTFTWWDRLFDTD 275
Query: 829 KGYRR 843
+ +
Sbjct: 276 SQFNK 280
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 187 bits (474), Expect = 1e-046
Identities = 96/254 (37%), Positives = 146/254 (57%), Gaps = 18/254 (7%)
Frame = +1
Query: 163 FLLHESVFFLSGLPFIYLERQGFLTKYKIQAKNNTPAVQEK--CITRLLLYHFCVNLPLM 336
FLLHE ++F LP+ +++ + ++K+Q P+ +E+ C+ +LL HF V +
Sbjct: 59 FLLHEFMYFFRCLPWFIIDQIPYFRRWKLQ-PTKIPSAKEQLYCLKSVLLSHFLVEAIPI 117
Query: 337 MASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHH 516
+P+ +G+ P PS K ++ +I +F++ED YW HR+ H YK +H HH
Sbjct: 118 WTFHPMCEKLGITVEVPFPSLKTMALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHH 177
Query: 517 EYATPFGLTSEYAHPAEILFLGFATIVGPAL----TGP-HLITLWLWMVLRVLETVEAHC 681
YA PFGL++EYAHPAE L LGF T+ P L TG HL TL +W+ LR+ + V++H
Sbjct: 178 RYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHS 237
Query: 682 GYHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKG-------YR 840
GY FPWSL+ +P + G++ HD HH GNY+S+F + D+ T+ G
Sbjct: 238 GYDFPWSLNKIMPFWAGAEHHDLHHHYFI---GNYASSFRWWDYCLDTESGPEAKASREE 294
Query: 841 RLKSLKENSDLKQT 882
R+K EN+ K+T
Sbjct: 295 RMKKRAENNAQKKT 308
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 175 bits (443), Expect = 5e-043
Identities = 93/239 (38%), Positives = 134/239 (56%), Gaps = 9/239 (3%)
Frame = +1
Query: 163 FLLHESVFFLSGLPFIYLERQGFLTKYKIQ-AKNNTPAVQEKCITRLLLYHFCVNLPLMM 339
FL HE +F LP+ ++R + K+KIQ K + Q +C+ +L HF V +
Sbjct: 59 FLTHEIFYFGRCLPWAIIDRIPYFRKWKIQDEKIPSDKEQWECLKSVLTSHFLVEAFPIW 118
Query: 340 ASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHHE 519
+P+ + +G+ P P ++ Q +F++ED YW HR LH YK +H HH
Sbjct: 119 FFHPLCQKIGISYQVPFPKITDMLIQWAVFFVLEDTWHYWFHRGLHYGVFYKYIHKQHHR 178
Query: 520 YATPFGLTSEYAHPAEILFLGFAT----IVGPALTGP-HLITLWLWMVLRVLETVEAHCG 684
YA PFGL +EYAHP E+ LG T IV +TG HL T+ +W++LR+ + V+AH G
Sbjct: 179 YAAPFGLAAEYAHPVEVALLGLGTVGIPIVWCLITGNLHLFTVSIWIILRLFQAVDAHSG 238
Query: 685 YHFPWSLSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKE 861
Y FPWSL NFLP + G+D HD HH G YSS+F + D+I T+ G + K ++
Sbjct: 239 YEFPWSLHNFLPFWAGADHHDEHHHYFI---GGYSSSFRWWDFILDTEAGPKAKKGRED 294
>sp|Q9GKT2|CE004_MACFA Uncharacterized protein C5orf4 homolog OS=Macaca
fascicularis GN=QccE-20373 PE=2 SV=1
Length = 333
Score = 128 bits (320), Expect = 9e-029
Identities = 70/219 (31%), Positives = 117/219 (53%), Gaps = 16/219 (7%)
Frame = +1
Query: 220 RQGFLTKYKIQAKNNTPA--VQEKCITRLLLYHFC-VNLPLMMASYPVFRAMGMRSSFPL 390
+ F+++Y+IQ N P V+ + R +L++ C V+ P+++ YP + G L
Sbjct: 106 KPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMVSFPMVVFLYPFLKWWGDPCRREL 165
Query: 391 PSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAE- 567
P++ ++ + +IE+ +FY+ HR+LH YK +H HHE+ P G+ S YAHP E
Sbjct: 166 PTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIEH 225
Query: 568 ILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 747
++ IVGP + G HL ++ +W L ++ T +HCGYH P FLP +FHD
Sbjct: 226 VVSNMLPAIVGPLVMGSHLSSITMWFSLALIITTISHCGYHLP-----FLP---SPEFHD 277
Query: 748 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKEN 864
YHH K +D + GTD +++ K+ +++
Sbjct: 278 YHH----LKFNQCYGVLGVLDHLHGTDTMFKQTKAYEKH 312
>sp|Q96IV6|CE004_HUMAN Uncharacterized protein C5orf4 OS=Homo sapiens GN=C5orf4
PE=1 SV=1
Length = 333
Score = 123 bits (307), Expect = 3e-027
Identities = 68/219 (31%), Positives = 113/219 (51%), Gaps = 16/219 (7%)
Frame = +1
Query: 220 RQGFLTKYKIQAKNNTPA--VQEKCITRLLLYHFC-VNLPLMMASYPVFRAMGMRSSFPL 390
+ F+++Y+IQ N P V+ + R +L++ C ++ P+++ YP + L
Sbjct: 106 KPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKWWRDPCRREL 165
Query: 391 PSWKEVSAQILFYFIIEDFVFYWGHRILHTKWLYKTVHSVHHEYATPFGLTSEYAHPAEI 570
P++ ++ + +IE+ +FY+ HR+LH YK +H HHE+ P G+ S YAHP E
Sbjct: 166 PTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIEH 225
Query: 571 LFLG-FATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 747
IVGP + G HL ++ +W L ++ T +HCGYH P FLP +FHD
Sbjct: 226 AVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGYHLP-----FLP---SPEFHD 277
Query: 748 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKEN 864
YHH K +D + GTD +++ K+ + +
Sbjct: 278 YHH----LKFNQCYGVLGVLDHLHGTDTMFKQTKAYERH 312
>sp|Q1LX59|C2512_DANRE Cholesterol 25-hydroxylase-like protein 1, member 2
OS=Danio rerio GN=ch25hl1.2 PE=3 SV=1
Length = 276
Score = 122 bits (304), Expect = 7e-027
Identities = 77/239 (32%), Positives = 122/239 (51%), Gaps = 25/239 (10%)
Frame = +1
Query: 166 LLHESVFFLSGLPFIYLERQG----FLTKYKIQA-KNNTPAVQEKCITRLLLYHFCVNLP 330
+L S +F+ LP++ + G + +YKIQ K T A+ C L H + +P
Sbjct: 43 VLTVSSYFVLVLPYLSCDILGRKWPAIYRYKIQPDKLPTTAMLLHCSGVTLYNHILLVIP 102
Query: 331 LMMASYPVFRAMGMRSSFPLPSWKEVSAQI-------LFYFIIEDFVFYWGHRILHTKWL 489
+A + R PLP ++ L F ++ F++++ H + +WL
Sbjct: 103 AAVAQWM------WRPPIPLPEQAPTLLELVGGVTGNLLLFDLQYFIWHFLHHKI--RWL 154
Query: 490 YKTVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETV 669
Y T H++HH Y+ PF L ++ E++ +GF T + P L HL+T W++MV+ V +V
Sbjct: 155 YVTFHAIHHNYSAPFALATQCLGGWELVTVGFWTTLNPVLLRCHLLTTWMFMVVHVYVSV 214
Query: 670 EAHCGYHFPWSLSNFLP--LYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKGYR 840
E HCGY FPWS S +P +YGG HD HH+ T N++ F + D +FGT +R
Sbjct: 215 EDHCGYDFPWSTSRLIPFGVYGGPSKHDVHHQKPNT---NFAPHFSHWDKMFGTHADFR 270
>sp|Q5PRC0|CH25H_DANRE Cholesterol 25-hydroxylase-like protein OS=Danio rerio
GN=ch25h PE=2 SV=1
Length = 251
Score = 115 bits (286), Expect = 8e-025
Identities = 74/227 (32%), Positives = 111/227 (48%), Gaps = 20/227 (8%)
Frame = +1
Query: 178 SVFFLSGLPFIYLE----RQGFLTKYKIQAKNNTP-AVQEKCITRLLLYHFCVNLPLMMA 342
+V+ LPF+ L+ + + +YKIQ K + + C+ L H PL +
Sbjct: 30 TVYLSFCLPFVLLDALSPKVELIRRYKIQQKASVSWTMMWSCLALSLYNHVVYIFPLSVL 89
Query: 343 SYPVFRAMGMRSSFP---LPSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSV 510
+ +R + + P W + +LF DF ++ H + H WLY+T H V
Sbjct: 90 HW-YWRPVSYLAEAPGVLRVVWDLAACLLLF-----DFQYFVWHLLHHKVPWLYRTFHKV 143
Query: 511 HHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYH 690
HH+Y + F L +EY+ E L LGF V P L G H +T L+ +L + +VE HCGY
Sbjct: 144 HHKYTSTFALATEYSGAWETLSLGFFAAVNPMLLGVHPMTEMLFHMLNMWLSVEDHCGYD 203
Query: 691 FPWSLSNFLP--LYGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 825
PW+ +P LYGG+ HD HH+ NY+ F + D +FGT
Sbjct: 204 LPWATHRLMPFGLYGGAPHHDVHHQKF---KSNYAPYFTHWDKLFGT 247
>sp|Q20027|C25HL_CAEEL Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
elegans GN=F35C8.5 PE=1 SV=1
Length = 300
Score = 112 bits (278), Expect = 7e-024
Identities = 54/158 (34%), Positives = 83/158 (52%), Gaps = 4/158 (2%)
Frame = +1
Query: 391 PSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVHHEYATPFGLTSEYAHPAE 567
P+ E+ +Q+ +F+ DF ++W H I H KWLY+ HSVHH Y++PF ++++ HP E
Sbjct: 125 PTVFEMLSQLAIFFLAFDFTYFWFHYINHKVKWLYRWCHSVHHMYSSPFAASAQHLHPFE 184
Query: 568 ILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 747
+ F+G P + H +T W+W + + E H GY FP++L Y G+ HD
Sbjct: 185 LFFVGTFITTIPWIFPTHCLTYWIWFFIAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHD 244
Query: 748 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKE 861
HH T + F Y+D + G Y LK + E
Sbjct: 245 MHHLRPLT---CFQPWFNYLDRLMGYHITYADLKKMTE 279
>sp|Q618G2|C25HL_CAEBR Cholesterol 25-hydroxylase-like protein OS=Caenorhabditis
briggsae GN=CBG14675 PE=3 SV=1
Length = 300
Score = 109 bits (270), Expect = 6e-023
Identities = 53/158 (33%), Positives = 81/158 (51%), Gaps = 4/158 (2%)
Frame = +1
Query: 391 PSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVHHEYATPFGLTSEYAHPAE 567
P+ E+ +Q+ +F+ DF ++W H H KWLY+ HSVHH Y++PF ++++ HP E
Sbjct: 125 PTVFEMVSQLAIFFLAFDFTYFWFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHLHPFE 184
Query: 568 ILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGSDFHD 747
+ F+ P + H +T WLW + + E H GY FP++L Y G+ HD
Sbjct: 185 LFFVATFITTVPWIFPTHCLTYWLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGAPAHD 244
Query: 748 YHHRLLYTKSGNYSSTFVYMDWIFGTDKGYRRLKSLKE 861
HH T + F Y+D + G Y LK + E
Sbjct: 245 MHHLRPLT---CFQPWFNYLDRLMGYHITYEDLKKMTE 279
>sp|O95992|CH25H_HUMAN Cholesterol 25-hydroxylase OS=Homo sapiens GN=CH25H PE=1
SV=1
Length = 272
Score = 96 bits (236), Expect = 5e-019
Identities = 66/218 (30%), Positives = 105/218 (48%), Gaps = 16/218 (7%)
Frame = +1
Query: 199 LPFIYLE----RQGFLTKYKIQAKNNTPAVQE--KCITRLLLYHFCVNLPLMMASYPVFR 360
LPF+ L+ L +YKI + +P+ Q+ C+ + L H P+ + +
Sbjct: 53 LPFVVLDILCSWVPALRRYKIH-PDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSP 111
Query: 361 AMGMRSSFPLPSWKEVSAQILFYFIIEDFVFYWGHRILH-TKWLYKTVHSVHHEYATPFG 537
A+ + P + ILF ++ D F+ H + H WLY+T H VHH+ ++ F
Sbjct: 112 ALLPHEA---PELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFA 168
Query: 538 LTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMVLRVLETVEAHCGYHFPWSLSNFL 717
L ++Y E+ LGF ++ L G H +T + V+ + +VE H GY+FPWS +
Sbjct: 169 LATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLV 228
Query: 718 PL--YGGSDFHDYHHRLLYTKSGNYSSTFVYMDWIFGT 825
P YGG HD HH + N++ F + D I GT
Sbjct: 229 PFGWYGGVVHHDLHHSHF---NCNFAPYFTHWDKILGT 263
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 185,567,659,578
Number of Sequences: 518415
Number of Extensions: 185567659578
Number of Successful Extensions: 1125298771
Number of sequences better than 0.0: 0
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