BLASTX 7.6.2
Query= UN50250 /QuerySize=721
(720 letters)
Database: GenBank nr;
15,229,318 sequences; 5,219,829,378 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|45935268|gb|AAS79669.1| glutamate decarboxylase 2 [Brassica j... 216 3e-054
gi|297838229|ref|XP_002886996.1| glutamate decarboxylase 2 [Arab... 211 9e-053
gi|186493337|ref|NP_001117556.1| glutamate decarboxylase 2 [Arab... 210 2e-052
gi|19698825|gb|AAL91148.1| glutamate decarboxylase, putative [Ar... 210 2e-052
gi|16226294|gb|AAL16126.1|AF428294_1 At1g65960/F12P19_12 [Arabid... 210 2e-052
gi|45935274|gb|AAS79672.1| glutamate decarboxylase 1 [Brassica j... 209 5e-052
gi|75268007|gb|ABA18652.1| glutamate decarboxylase [Populus trem... 208 8e-052
gi|224079722|ref|XP_002305927.1| predicted protein [Populus tric... 208 8e-052
gi|70609690|gb|AAZ05070.1| glutamate decarboxylase [Citrus sinen... 207 1e-051
gi|226532026|ref|NP_001150918.1| LOC100284551 [Zea mays] 206 4e-051
gi|147835041|emb|CAN67952.1| hypothetical protein VITISV_028884 ... 205 5e-051
gi|225424524|ref|XP_002285267.1| PREDICTED: hypothetical protein... 205 5e-051
gi|255575222|ref|XP_002528515.1| glutamate decarboxylase, putati... 205 5e-051
gi|297737555|emb|CBI26756.3| unnamed protein product [Vitis vini... 205 5e-051
gi|297807755|ref|XP_002871761.1| hypothetical protein ARALYDRAFT... 205 5e-051
gi|224120428|ref|XP_002318327.1| predicted protein [Populus tric... 205 7e-051
gi|255561763|ref|XP_002521891.1| glutamate decarboxylase, putati... 205 7e-051
gi|242075968|ref|XP_002447920.1| hypothetical protein SORBIDRAFT... 205 9e-051
gi|125543034|gb|EAY89173.1| hypothetical protein OsI_10669 [Oryz... 204 1e-050
gi|15237949|ref|NP_197235.1| glutamate decarboxylase 1 [Arabidop... 204 2e-050
>gi|45935268|gb|AAS79669.1| glutamate decarboxylase 2 [Brassica juncea]
Length = 494
Score = 216 bits (549), Expect = 3e-054
Identities = 105/106 (99%), Positives = 106/106 (100%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDL 319
VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFED+
Sbjct: 176 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDV 221
>gi|297838229|ref|XP_002886996.1| glutamate decarboxylase 2 [Arabidopsis lyrata
subsp. lyrata]
Length = 494
Score = 211 bits (536), Expect = 9e-053
Identities = 102/106 (96%), Positives = 105/106 (99%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQN+RKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 115 ETAVGVGTVGSSEAIMLAGLAFKRKWQNRRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 174
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDL 319
VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFED+
Sbjct: 175 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDV 220
>gi|186493337|ref|NP_001117556.1| glutamate decarboxylase 2 [Arabidopsis
thaliana]
Length = 494
Score = 210 bits (534), Expect = 2e-052
Identities = 102/106 (96%), Positives = 104/106 (98%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 115 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 174
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDL 319
VELKEV LSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFED+
Sbjct: 175 VELKEVNLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDV 220
>gi|19698825|gb|AAL91148.1| glutamate decarboxylase, putative [Arabidopsis
thaliana]
Length = 419
Score = 210 bits (534), Expect = 2e-052
Identities = 102/106 (96%), Positives = 104/106 (98%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 40 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 99
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDL 319
VELKEV LSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFED+
Sbjct: 100 VELKEVNLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDV 145
>gi|16226294|gb|AAL16126.1|AF428294_1 At1g65960/F12P19_12 [Arabidopsis
thaliana]
Length = 494
Score = 210 bits (534), Expect = 2e-052
Identities = 102/106 (96%), Positives = 104/106 (98%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 115 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 174
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDL 319
VELKEV LSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFED+
Sbjct: 175 VELKEVNLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDV 220
>gi|45935274|gb|AAS79672.1| glutamate decarboxylase 1 [Brassica juncea]
Length = 262
Score = 209 bits (530), Expect = 5e-052
Identities = 101/109 (92%), Positives = 106/109 (97%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKP+DKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKLSEGYYVMDP+KA EMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLSEGYYVMDPEKAVEMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|75268007|gb|ABA18652.1| glutamate decarboxylase [Populus tremula x Populus
alba]
Length = 499
Score = 208 bits (528), Expect = 8e-052
Identities = 100/109 (91%), Positives = 106/109 (97%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKLS+GYYVMDP+KA +MVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLSDGYYVMDPEKAVQMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|224079722|ref|XP_002305927.1| predicted protein [Populus trichocarpa]
Length = 498
Score = 208 bits (528), Expect = 8e-052
Identities = 100/109 (91%), Positives = 106/109 (97%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKLS+GYYVMDP+KA +MVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLSDGYYVMDPEKAVQMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|70609690|gb|AAZ05070.1| glutamate decarboxylase [Citrus sinensis]
Length = 494
Score = 207 bits (526), Expect = 1e-051
Identities = 101/109 (92%), Positives = 105/109 (96%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKP+DKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPFDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKLSEGYYVMDP KA EMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLSEGYYVMDPAKAVEMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|226532026|ref|NP_001150918.1| LOC100284551 [Zea mays]
Length = 489
Score = 206 bits (522), Expect = 4e-051
Identities = 100/109 (91%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNK KA GKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 118 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAAGKPYDKPNIVTGANVQVCWEKFARYFE 177
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL EGYYVMDP+KAAEMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 178 VELKEVKLKEGYYVMDPEKAAEMVDENTICVAAILGSTLNGEFEDVKLL 226
>gi|147835041|emb|CAN67952.1| hypothetical protein VITISV_028884 [Vitis
vinifera]
Length = 489
Score = 205 bits (521), Expect = 5e-051
Identities = 99/109 (90%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
E A+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 110 EAAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 169
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL +GYYVMDP+KA EMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 170 VELKEVKLRDGYYVMDPEKAVEMVDENTICVAAILGSTLNGEFEDVKLL 218
>gi|225424524|ref|XP_002285267.1| PREDICTED: hypothetical protein [Vitis
vinifera]
Length = 495
Score = 205 bits (521), Expect = 5e-051
Identities = 99/109 (90%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
E A+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 EAAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL +GYYVMDP+KA EMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLRDGYYVMDPEKAVEMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|255575222|ref|XP_002528515.1| glutamate decarboxylase, putative [Ricinus
communis]
Length = 498
Score = 205 bits (521), Expect = 5e-051
Identities = 100/109 (91%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL +GYYVMDP KA EMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLRDGYYVMDPVKAVEMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|297737555|emb|CBI26756.3| unnamed protein product [Vitis vinifera]
Length = 462
Score = 205 bits (521), Expect = 5e-051
Identities = 99/109 (90%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
E A+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 EAAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL +GYYVMDP+KA EMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLRDGYYVMDPEKAVEMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|297807755|ref|XP_002871761.1| hypothetical protein ARALYDRAFT_488599
[Arabidopsis lyrata subsp. lyrata]
Length = 502
Score = 205 bits (521), Expect = 5e-051
Identities = 100/109 (91%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKP DKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPVDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKLSEGYYVMDP KA +MVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLSEGYYVMDPQKAVDMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|224120428|ref|XP_002318327.1| predicted protein [Populus trichocarpa]
Length = 501
Score = 205 bits (520), Expect = 7e-051
Identities = 100/109 (91%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL+EGYYVMDP KA EMVDENTICVAAILGSTL GEFED+ L+
Sbjct: 176 VELKEVKLTEGYYVMDPVKAVEMVDENTICVAAILGSTLTGEFEDVKLL 224
>gi|255561763|ref|XP_002521891.1| glutamate decarboxylase, putative [Ricinus
communis]
Length = 297
Score = 205 bits (520), Expect = 7e-051
Identities = 100/109 (91%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
E A+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 EAAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL EGYYVMDP +AAEMVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLKEGYYVMDPVQAAEMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|242075968|ref|XP_002447920.1| hypothetical protein SORBIDRAFT_06g018050
[Sorghum bicolor]
Length = 488
Score = 205 bits (519), Expect = 9e-051
Identities = 99/109 (90%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNK KA GKPYDKPNIVTGANVQVCWEKFARYFE
Sbjct: 118 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAAGKPYDKPNIVTGANVQVCWEKFARYFE 177
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL EGYYVMDP+KAAEMVDENTICVAAILGSTLNGEFED+ ++
Sbjct: 178 VELKEVKLREGYYVMDPEKAAEMVDENTICVAAILGSTLNGEFEDVKML 226
>gi|125543034|gb|EAY89173.1| hypothetical protein OsI_10669 [Oryza sativa Indica
Group]
Length = 492
Score = 204 bits (518), Expect = 1e-050
Identities = 97/109 (88%), Positives = 105/109 (96%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKP+DKPNI+TGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRRWQNKRKAEGKPFDKPNIITGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKL +GYYVMDP+KA +MVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLRDGYYVMDPEKAVDMVDENTICVAAILGSTLNGEFEDVKLL 224
>gi|15237949|ref|NP_197235.1| glutamate decarboxylase 1 [Arabidopsis thaliana]
Length = 502
Score = 204 bits (517), Expect = 2e-050
Identities = 99/109 (90%), Positives = 104/109 (95%)
Frame = +2
Query: 2 ETAMGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFARYFE 181
ETA+GVGTVGSSEAIMLAGLAFKR WQNKRKAEGKP DKPNIVTGANVQVCWEKFARYFE
Sbjct: 116 ETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPVDKPNIVTGANVQVCWEKFARYFE 175
Query: 182 VELKEVKLSEGYYVMDPDKAAEMVDENTICVAAILGSTLNGEFEDLWLI 328
VELKEVKLSEGYYVMDP +A +MVDENTICVAAILGSTLNGEFED+ L+
Sbjct: 176 VELKEVKLSEGYYVMDPQQAVDMVDENTICVAAILGSTLNGEFEDVKLL 224
Database: GenBank nr
Posted date: Thu Sep 08 23:06:31 2011
Number of letters in database: 5,219,829,378
Number of sequences in database: 15,229,318
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,378,952,789,192
Number of Sequences: 15229318
Number of Extensions: 5378952789192
Number of Successful Extensions: 1252460229
Number of sequences better than 0.0: 0
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