BLASTX 7.6.2
Query= UN54559 /QuerySize=844
(843 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O94298|YOOF_SCHPO Uncharacterized hydroxylase C887.15c OS=Sch... 116 3e-025
sp|P38992|SUR2_YEAST Sphingolipid C4-hydroxylase SUR2 OS=Sacchar... 102 3e-021
sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens G... 64 2e-009
sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii G... 64 2e-009
sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicu... 63 2e-009
sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN... 61 1e-008
sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus G... 61 1e-008
sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC... 61 1e-008
sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albica... 60 2e-008
sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicu... 60 2e-008
sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus ... 59 4e-008
sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces ... 59 5e-008
sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G027... 52 3e-006
sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharo... 52 3e-006
sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G026... 52 5e-006
>sp|O94298|YOOF_SCHPO Uncharacterized hydroxylase C887.15c
OS=Schizosaccharomyces pombe GN=SPBC887.15c PE=1 SV=1
Length = 293
Score = 116 bits (289), Expect = 3e-025
Identities = 48/95 (50%), Positives = 70/95 (73%)
Frame = +1
Query: 469 FSLLLLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLE 648
++ ++ A Q+ A +ID+WQYF HRY+H NK LY IH+ HHRL VPY+ GALYNHP E
Sbjct: 125 YNFIVPAFQYFFAFFIIDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFE 184
Query: 649 SLLLDTVSGALSFLFSGMSPRTAIFFFSFATIKTL 753
L+LDT +++L +G+SP+ A+ FF+ +T+KT+
Sbjct: 185 GLILDTFGAGVAYLAAGLSPQQAVIFFTLSTLKTV 219
>sp|P38992|SUR2_YEAST Sphingolipid C4-hydroxylase SUR2 OS=Saccharomyces
cerevisiae GN=SUR2 PE=1 SV=1
Length = 349
Score = 102 bits (254), Expect = 3e-021
Identities = 46/102 (45%), Positives = 66/102 (64%)
Frame = +1
Query: 448 DASTTQQFSLLLLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGA 627
DA+ + + A + L +DTWQYF+HR MH+NK LYK HS HH L VPY+YGA
Sbjct: 145 DAAIYYGYMYGMSALKIFAGFLFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPYAYGA 204
Query: 628 LYNHPLESLLLDTVSGALSFLFSGMSPRTAIFFFSFATIKTL 753
L+N+P+E LLDT+ ++ + ++ R I F+FAT+KT+
Sbjct: 205 LFNNPVEGFLLDTLGTGIAMTLTHLTHREQIILFTFATMKTV 246
>sp|Q15800|ERG25_HUMAN C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1
SV=1
Length = 293
Score = 64 bits (153), Expect = 2e-009
Identities = 41/99 (41%), Positives = 57/99 (57%), Gaps = 7/99 (7%)
Frame = +1
Query: 481 LLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLL 660
LLAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE+L+L
Sbjct: 138 LLARCFGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLIL 195
Query: 661 DTVSGALSFLFSGMSPRTAIFFFSFATIKTL-TITVDSG 774
G F+ + I +++ TI+ L TI V SG
Sbjct: 196 ----GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSG 230
>sp|Q5R574|ERG25_PONAB C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2
SV=1
Length = 293
Score = 64 bits (153), Expect = 2e-009
Identities = 41/99 (41%), Positives = 57/99 (57%), Gaps = 7/99 (7%)
Frame = +1
Query: 481 LLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLL 660
LLAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE+L+L
Sbjct: 138 LLARCFGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLIL 195
Query: 661 DTVSGALSFLFSGMSPRTAIFFFSFATIKTL-TITVDSG 774
G F+ + I +++ TI+ L TI V SG
Sbjct: 196 ----GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSG 230
>sp|Q4R4Q4|ERG25_MACFA C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL
PE=2 SV=1
Length = 293
Score = 63 bits (152), Expect = 2e-009
Identities = 41/99 (41%), Positives = 57/99 (57%), Gaps = 7/99 (7%)
Frame = +1
Query: 481 LLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLL 660
LLAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE+L+L
Sbjct: 138 LLARCFGCA-VIEDTWHYFMHRLLH-HKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLIL 195
Query: 661 DTVSGALSFLFSGMSPRTAIFFFSFATIKTL-TITVDSG 774
G F+ + I +++ TI+ L TI V SG
Sbjct: 196 ----GTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSG 230
>sp|Q7ZW77|ERG25_DANRE C-4 methylsterol oxidase OS=Danio rerio GN=sc4mol PE=2
SV=1
Length = 291
Score = 61 bits (146), Expect = 1e-008
Identities = 39/99 (39%), Positives = 58/99 (58%), Gaps = 7/99 (7%)
Frame = +1
Query: 481 LLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLL 660
LLA+ F A ++ DTW YF+HR +H ++ +YK+IH HH P+ A Y HPLE+L+L
Sbjct: 138 LLAQCFGCA-VIEDTWHYFLHRALH-HRRIYKYIHKVHHDFTSPFGMQAEYAHPLETLIL 195
Query: 661 DTVSGALSFLFSGMSPRTAIFFFSFATIKTL-TITVDSG 774
GA F+ + + I +++ T + L TI V SG
Sbjct: 196 ----GAGFFIGTMVFCNHMILLWAWVTFRLLETIDVHSG 230
>sp|Q9CRA4|ERG25_MOUSE C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2
SV=1
Length = 293
Score = 61 bits (146), Expect = 1e-008
Identities = 35/89 (39%), Positives = 51/89 (57%), Gaps = 6/89 (6%)
Frame = +1
Query: 511 LVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLLDTVSGALSFL 690
++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE+L+L G F+
Sbjct: 147 VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLIL----GTGFFI 201
Query: 691 FSGMSPRTAIFFFSFATIKTL-TITVDSG 774
+ I +++ TI+ L TI V SG
Sbjct: 202 GIVLLCDHVILLWAWVTIRLLETIDVHSG 230
>sp|Q6UGB2|ERG25_PIG C-4 methylsterol oxidase OS=Sus scrofa GN=SC4MOL PE=2 SV=1
Length = 293
Score = 61 bits (146), Expect = 1e-008
Identities = 38/100 (38%), Positives = 57/100 (57%), Gaps = 7/100 (7%)
Frame = +1
Query: 478 LLLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLL 657
+ LAR F A ++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE+L+
Sbjct: 137 IALARCFGCA-VIEDTWHYFLHRLLH-HKRIYKYIHKIHHEFQAPFGMEAEYAHPLETLI 194
Query: 658 LDTVSGALSFLFSGMSPRTAIFFFSFATIKTL-TITVDSG 774
L G F+ + I +++ T++ + TI V SG
Sbjct: 195 L----GTGFFIGIMLLCDHVILLWAWVTVRLIETIDVHSG 230
>sp|O59933|ERG25_CANAL C-4 methylsterol oxidase OS=Candida albicans GN=ERG25
PE=3 SV=1
Length = 308
Score = 60 bits (143), Expect = 2e-008
Identities = 32/95 (33%), Positives = 49/95 (51%), Gaps = 1/95 (1%)
Frame = +1
Query: 493 QFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLLDTVS 672
Q+ + ++ DTW Y+ HR +H F YK+IH QHHR P+ A Y HP+E LL +
Sbjct: 144 QWAVFFVLEDTWHYWFHRGLHYGVF-YKYIHKQHHRYAAPFGLAAEYAHPVEVALLGLGT 202
Query: 673 GALSFLFSGMSPRTAIFFFSFATIKTLTITVDSGS 777
+ ++ ++ +F S I L VD+ S
Sbjct: 203 VGIPIVWCLITGNLHLFTVSIWIILRLFQAVDAHS 237
>sp|O35532|ERG25_RAT C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol
PE=2 SV=1
Length = 293
Score = 60 bits (143), Expect = 2e-008
Identities = 34/89 (38%), Positives = 51/89 (57%), Gaps = 6/89 (6%)
Frame = +1
Query: 511 LVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLLDTVSGALSFL 690
++ DTW YF+HR +H +K +YK+IH HH P+ A Y HPLE+L+L G F+
Sbjct: 147 VIEDTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLIL----GTGFFI 201
Query: 691 FSGMSPRTAIFFFSFATIKTL-TITVDSG 774
+ I +++ T++ L TI V SG
Sbjct: 202 GIVLLCDHVILLWAWVTMRLLETIDVHSG 230
>sp|Q5ZLL6|ERG25_CHICK C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2
SV=1
Length = 296
Score = 59 bits (141), Expect = 4e-008
Identities = 28/61 (45%), Positives = 41/61 (67%), Gaps = 2/61 (3%)
Frame = +1
Query: 478 LLLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLL 657
+LLA+ F A ++ D W YF+HR +H +K +YK+IH HH + P+ A Y HPLE+L+
Sbjct: 137 VLLAQCFGCA-VIEDAWHYFLHRLLH-HKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLI 194
Query: 658 L 660
L
Sbjct: 195 L 195
>sp|P53045|ERG25_YEAST C-4 methylsterol oxidase OS=Saccharomyces cerevisiae
GN=ERG25 PE=1 SV=1
Length = 309
Score = 59 bits (140), Expect = 5e-008
Identities = 34/102 (33%), Positives = 49/102 (48%), Gaps = 1/102 (0%)
Frame = +1
Query: 472 SLLLLARQFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLES 651
SL +A + + ++ DTW Y+ HR H F YK+IH QHHR P+ A Y HP E+
Sbjct: 137 SLKTMALEIGLFFVLEDTWHYWAHRLFHYGVF-YKYIHKQHHRYAAPFGLSAEYAHPAET 195
Query: 652 LLLDTVSGALSFLFSGMSPRTAIFFFSFATIKTLTITVDSGS 777
L L + + L+ + + +F L VDS S
Sbjct: 196 LSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHS 237
>sp|Q55D52|ER25A_DICDI Putative C-4 methylsterol oxidase DDB_G0270946
OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2
Length = 267
Score = 52 bits (124), Expect = 3e-006
Identities = 49/170 (28%), Positives = 75/170 (44%), Gaps = 21/170 (12%)
Frame = +1
Query: 223 DEFL---GTFVPILVYWVYSGMYI------CLGSLERYRLHSKVDEDEKNLVTKSAVVKG 375
+EFL GTF+ V+ Y G +I + +Y++ + D K+ +K
Sbjct: 22 EEFLLTYGTFIAHQVF--YFGCFIPFLIADFIPFFRKYKIQQTKENDWKS--QTYCAIKV 77
Query: 376 VLLQQALQAIISVIL---FKITGSDDADASTTQQFSLLLLARQFIIAMLVIDTWQYFIHR 546
+L Q +Q + I K G + + LL L FII D + Y+ HR
Sbjct: 78 ILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIE----DFYFYWAHR 133
Query: 547 YMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLLDTVSGALSFLFS 696
+H + YK+IH HH P+ A Y HPLE+++L + FLFS
Sbjct: 134 ALH-HGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFS 182
>sp|Q9UUH4|ERG25_SCHPO C-4 methylsterol oxidase OS=Schizosaccharomyces pombe
GN=erg25 PE=2 SV=1
Length = 300
Score = 52 bits (124), Expect = 3e-006
Identities = 24/56 (42%), Positives = 30/56 (53%), Gaps = 1/56 (1%)
Frame = +1
Query: 493 QFIIAMLVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLL 660
Q + + DTW Y+ HR H F Y+ IH HHR P+ A Y HPLE +LL
Sbjct: 138 QITLFFFLEDTWHYWAHRLFHYGIF-YRFIHKVHHRYSAPFGLSAEYAHPLEIILL 192
>sp|Q55D54|ER25B_DICDI Putative C-4 methylsterol oxidase DDB_G0269788
OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1
Length = 270
Score = 52 bits (123), Expect = 5e-006
Identities = 26/62 (41%), Positives = 37/62 (59%), Gaps = 1/62 (1%)
Frame = +1
Query: 511 LVIDTWQYFIHRYMHINKFLYKHIHSQHHRLIVPYSYGALYNHPLESLLLDTVSGALSFL 690
L+ D + Y++HR +H + F YKHIH HH P+ A Y HPLE+++L + FL
Sbjct: 122 LIEDFYFYWVHRALH-HGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFL 180
Query: 691 FS 696
FS
Sbjct: 181 FS 182
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 198,593,781,018
Number of Sequences: 518415
Number of Extensions: 198593781018
Number of Successful Extensions: 1207178190
Number of sequences better than 0.0: 0
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