BLASTX 7.6.2
Query= UN60303 /QuerySize=757
(756 letters)
Database: UniProt/TrEMBL;
11,397,958 sequences; 3,661,877,547 total letters
Score E
Sequences producing significant alignments: (bits) Value
tr|O23954|O23954_GOSHI Endo-1,4-beta-glucanase (Fragment) OS=Gos... 144 1e-032
tr|Q25B23|Q25B23_PRUPE Endo-beta-1,4-glucanase OS=Prunus persica... 142 4e-032
tr|Q8GTP5|Q8GTP5_PYRCO Endo-1,4-beta-D-glucanase OS=Pyrus commun... 142 4e-032
tr|Q8LJP6|Q8LJP6_GOSHI Endo-1,4-beta-glucanase OS=Gossypium hirs... 142 4e-032
tr|O65186|O65186_FRAAN Cellulase OS=Fragaria ananassa GN=Cel2 PE... 141 7e-032
tr|Q8L6S6|Q8L6S6_FRAAN Endo-beta-1,4-glucanase OS=Fragaria anana... 141 7e-032
tr|Q9XGK7|Q9XGK7_FRAAN Endo-beta-1,4-glucanase OS=Fragaria anana... 141 7e-032
tr|B9GL66|B9GL66_POPTR Predicted protein OS=Populus trichocarpa ... 141 9e-032
tr|A9PIJ6|A9PIJ6_POPTR Putative uncharacterized protein OS=Popul... 141 1e-031
tr|B9GXH8|B9GXH8_POPTR Glycosyl hydrolase family 9 OS=Populus tr... 141 1e-031
tr|Q25B24|Q25B24_PRUPE Endo-beta-1,4-glucanase OS=Prunus persica... 141 1e-031
tr|Q2HZY0|Q2HZY0_SOYBN Endo-1,4-beta-glucanase (Fragment) OS=Gly... 137 2e-030
tr|A5BTQ3|A5BTQ3_VITVI Putative uncharacterized protein OS=Vitis... 133 2e-029
tr|Q9ZSP9|Q9ZSP9_SOLLC Endo-beta-1,4-D-glucanase OS=Solanum lyco... 129 4e-028
tr|Q93WY9|Q93WY9_TOBAC Endo-beta-1,4-glucanase OS=Nicotiana taba... 125 7e-027
tr|A6N4B9|A6N4B9_MANIN Endo-beta-1,4-glucanase OS=Mangifera indi... 103 2e-020
tr|A2WM56|A2WM56_ORYSI Putative uncharacterized protein OS=Oryza... 96 4e-018
tr|C5YUQ8|C5YUQ8_SORBI Putative uncharacterized protein Sb09g006... 92 5e-017
tr|C0HF28|C0HF28_MAIZE Putative uncharacterized protein OS=Zea m... 89 4e-016
tr|C0P2V6|C0P2V6_MAIZE Putative uncharacterized protein OS=Zea m... 89 4e-016
>tr|O23954|O23954_GOSHI Endo-1,4-beta-glucanase (Fragment) OS=Gossypium hirsutum
PE=2 SV=1
Length = 324
Score = 144 bits (361), Expect = 1e-032
Identities = 66/104 (63%), Positives = 82/104 (78%)
Frame = -3
Query: 748 RVTSPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWG 569
R +P +S P+ I QKMT SW KG+TYYRYST V NKS + LK+L LSI LYGPIWG
Sbjct: 221 RTPTPASSSSPITINQKMTTSWNHKGKTYYRYSTVVTNKSYKTLKALKLSISKLYGPIWG 280
Query: 568 LSRSGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
L++SGNS+G P W++SL +GKSLEFVYIH+ SPA+V+VSSY +A
Sbjct: 281 LTKSGNSYGFPEWLNSLPAGKSLEFVYIHAASPADVSVSSYNIA 324
>tr|Q25B23|Q25B23_PRUPE Endo-beta-1,4-glucanase OS=Prunus persica GN=eg4 PE=2
SV=1
Length = 620
Score = 142 bits (357), Expect = 4e-032
Identities = 66/101 (65%), Positives = 83/101 (82%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P +S P+AI QK+T SWVSKG TYYRYST V NKS + L +L LS+ LYGPIWGL++
Sbjct: 520 APASSSSPIAIQQKLTTSWVSKGVTYYRYSTVVTNKSAKTLTNLKLSVSKLYGPIWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+G+S+ PSW++SL +GKSLEFVYIHS SPA+VAVSSY+LA
Sbjct: 580 AGDSYVFPSWINSLSAGKSLEFVYIHSASPADVAVSSYSLA 620
>tr|Q8GTP5|Q8GTP5_PYRCO Endo-1,4-beta-D-glucanase OS=Pyrus communis GN=PC-EG2
PE=2 SV=1
Length = 620
Score = 142 bits (357), Expect = 4e-032
Identities = 66/101 (65%), Positives = 80/101 (79%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
SP +S P+AI Q+ T SW+SKG TYYRYS V NKS + L +L LSI LYGPIWGL++
Sbjct: 520 SPAPSSSPIAISQRKTSSWISKGVTYYRYSAIVTNKSAKELTNLKLSISKLYGPIWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+GNS+G PSW++SL GKS+EFVYIHS SPANV VSSY+LA
Sbjct: 580 AGNSYGFPSWINSLPVGKSMEFVYIHSASPANVLVSSYSLA 620
>tr|Q8LJP6|Q8LJP6_GOSHI Endo-1,4-beta-glucanase OS=Gossypium hirsutum GN=Cel1
PE=2 SV=2
Length = 620
Score = 142 bits (357), Expect = 4e-032
Identities = 66/101 (65%), Positives = 80/101 (79%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
SP +S P+AI Q+ T SW+SKG TYYRYS V NKS + L +L LSI LYGPIWGL++
Sbjct: 520 SPAPSSSPIAISQRKTSSWISKGVTYYRYSAIVTNKSAKELTNLKLSISKLYGPIWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+GNS+G PSW++SL GKS+EFVYIHS SPANV VSSY+LA
Sbjct: 580 AGNSYGFPSWINSLPVGKSMEFVYIHSASPANVLVSSYSLA 620
>tr|O65186|O65186_FRAAN Cellulase OS=Fragaria ananassa GN=Cel2 PE=2 SV=2
Length = 620
Score = 141 bits (355), Expect = 7e-032
Identities = 65/101 (64%), Positives = 82/101 (81%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P SGP+AI QK+T SWVSKG TYYRYSTTV NKS + L +L L+I LYGP+WGL++
Sbjct: 520 APASTSGPIAIAQKVTSSWVSKGVTYYRYSTTVTNKSGKTLNNLKLTISKLYGPLWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+G+S+ PSW++SL +GKSLEFVYIH+ S ANV VSSY+LA
Sbjct: 580 TGDSYVFPSWLNSLPAGKSLEFVYIHAASAANVLVSSYSLA 620
>tr|Q8L6S6|Q8L6S6_FRAAN Endo-beta-1,4-glucanase OS=Fragaria ananassa GN=faEG3
PE=4 SV=1
Length = 620
Score = 141 bits (355), Expect = 7e-032
Identities = 65/101 (64%), Positives = 82/101 (81%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P SGP+AI QK+T SWVSKG TYYRYSTTV NKS + L +L L+I LYGP+WGL++
Sbjct: 520 APASTSGPIAIAQKVTSSWVSKGVTYYRYSTTVTNKSGKTLNNLKLTISKLYGPLWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+G+S+ PSW++SL +GKSLEFVYIH+ S ANV VSSY+LA
Sbjct: 580 TGDSYVFPSWLNSLPAGKSLEFVYIHAASAANVLVSSYSLA 620
>tr|Q9XGK7|Q9XGK7_FRAAN Endo-beta-1,4-glucanase OS=Fragaria ananassa GN=eg3 PE=2
SV=1
Length = 620
Score = 141 bits (355), Expect = 7e-032
Identities = 65/101 (64%), Positives = 82/101 (81%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P SGP+AI QK+T SWVSKG TYYRYSTTV NKS + L +L L+I LYGP+WGL++
Sbjct: 520 APASTSGPIAIAQKVTSSWVSKGVTYYRYSTTVTNKSGKTLNNLKLTISKLYGPLWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+G+S+ PSW++SL +GKSLEFVYIH+ S ANV VSSY+LA
Sbjct: 580 TGDSYVFPSWLNSLPAGKSLEFVYIHAASAANVLVSSYSLA 620
>tr|B9GL66|B9GL66_POPTR Predicted protein OS=Populus trichocarpa
GN=POPTRDRAFT_751215 PE=4 SV=1
Length = 622
Score = 141 bits (354), Expect = 9e-032
Identities = 64/101 (63%), Positives = 80/101 (79%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P S PVAIVQK T +W++KG+TYYRYST V NKS + LK + LSI LYGP+WGL++
Sbjct: 522 APASTSAPVAIVQKTTATWIAKGKTYYRYSTIVTNKSAKELKDIKLSISKLYGPLWGLTK 581
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
SGN + PSW+ SL +GKSLEFVY+HS S A+V+VSSYTLA
Sbjct: 582 SGNFYAFPSWITSLAAGKSLEFVYVHSASAADVSVSSYTLA 622
>tr|A9PIJ6|A9PIJ6_POPTR Putative uncharacterized protein OS=Populus trichocarpa
PE=2 SV=1
Length = 280
Score = 141 bits (353), Expect = 1e-031
Identities = 62/100 (62%), Positives = 80/100 (80%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P S P++I QKMT +W++KG+TYYRYS TV NKS + LK + LSI LYGP+WGL++
Sbjct: 180 APASTSAPISIAQKMTTTWIAKGKTYYRYSITVTNKSAKELKDIKLSISKLYGPLWGLTK 239
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
SGNS+ PSWM SL +GKSLEFVYIHS S A+++VSSY+L
Sbjct: 240 SGNSYAFPSWMSSLPAGKSLEFVYIHSASAADISVSSYSL 279
>tr|B9GXH8|B9GXH8_POPTR Glycosyl hydrolase family 9 OS=Populus trichocarpa
GN=POPTRDRAFT_554485 PE=4 SV=1
Length = 622
Score = 141 bits (353), Expect = 1e-031
Identities = 62/100 (62%), Positives = 80/100 (80%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P S P++I QKMT +W++KG+TYYRYS TV NKS + LK + LSI LYGP+WGL++
Sbjct: 522 APASTSAPISIAQKMTTTWIAKGKTYYRYSITVTNKSAKELKDIKLSISKLYGPLWGLTK 581
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
SGNS+ PSWM SL +GKSLEFVYIHS S A+++VSSY+L
Sbjct: 582 SGNSYAFPSWMSSLPAGKSLEFVYIHSASAADISVSSYSL 621
>tr|Q25B24|Q25B24_PRUPE Endo-beta-1,4-glucanase OS=Prunus persica GN=eg4 PE=3
SV=1
Length = 620
Score = 141 bits (353), Expect = 1e-031
Identities = 66/101 (65%), Positives = 82/101 (81%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P +S P+AI QK+T SWVSKG TYYRYST V NKS + L +L LS+ LYGPIWGL++
Sbjct: 520 APASSSSPIAIQQKLTTSWVSKGVTYYRYSTVVTNKSAKTLTNLKLSVSKLYGPIWGLTK 579
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
+G+S+ PSW++SL +GKSLEFVYIHS SPA VAVSSY+LA
Sbjct: 580 AGDSYVFPSWINSLSAGKSLEFVYIHSASPAVVAVSSYSLA 620
>tr|Q2HZY0|Q2HZY0_SOYBN Endo-1,4-beta-glucanase (Fragment) OS=Glycine max
GN=cel8 PE=2 SV=1
Length = 602
Score = 137 bits (343), Expect = 2e-030
Identities = 65/106 (61%), Positives = 83/106 (78%), Gaps = 1/106 (0%)
Frame = -3
Query: 751 PRVTSPVRA-SGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPI 575
P+ T P + SGP++I QKMT SW++ G TYYRYST V NKS + L SLNLSI LYGPI
Sbjct: 497 PKTTPPPASWSGPISIEQKMTTSWIAHGITYYRYSTVVTNKSNKSLSSLNLSISKLYGPI 556
Query: 574 WGLSRSGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
WG+++SG+S+ PSW+ SL +GKSLEFVYIHS S A+V+V++Y LA
Sbjct: 557 WGVTKSGDSYTFPSWLSSLSAGKSLEFVYIHSASAADVSVTNYVLA 602
>tr|A5BTQ3|A5BTQ3_VITVI Putative uncharacterized protein OS=Vitis vinifera
GN=VITISV_031862 PE=4 SV=1
Length = 629
Score = 133 bits (334), Expect = 2e-029
Identities = 63/106 (59%), Positives = 80/106 (75%), Gaps = 1/106 (0%)
Frame = -3
Query: 751 PRVT-SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPI 575
P+VT +P S +AI QK+T SW +KG TY RYST V NKS + LK L L+I LYGP+
Sbjct: 524 PKVTPAPASTSAEIAIEQKLTTSWKAKGNTYNRYSTIVTNKSAKTLKDLELTISKLYGPL 583
Query: 574 WGLSRSGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTLA 437
WGL++SG+S+G PSW+ +L +G+ LEFVYIHS PA V+VSSYTLA
Sbjct: 584 WGLTKSGDSYGFPSWLKALAAGEKLEFVYIHSADPAEVSVSSYTLA 629
>tr|Q9ZSP9|Q9ZSP9_SOLLC Endo-beta-1,4-D-glucanase OS=Solanum lycopersicum
GN=Cel8 PE=2 SV=1
Length = 625
Score = 129 bits (323), Expect = 4e-028
Identities = 63/105 (60%), Positives = 79/105 (75%), Gaps = 1/105 (0%)
Frame = -3
Query: 754 KPRVTSPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPI 575
+PRV PV A+ V I Q+ T SW G+TYYRYS V NKS + +K+L LSI LYGP+
Sbjct: 521 RPRVL-PVPANAHVTIQQRATSSWALNGKTYYRYSAVVTNKSGKTVKNLKLSIVKLYGPL 579
Query: 574 WGLSRSGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
WGL++ GNSF P+W++SL +GKSLEFVYIH+ SPA V+VSSYTL
Sbjct: 580 WGLTKYGNSFIFPAWLNSLPAGKSLEFVYIHTASPAIVSVSSYTL 624
>tr|Q93WY9|Q93WY9_TOBAC Endo-beta-1,4-glucanase OS=Nicotiana tabacum GN=Cel8
PE=2 SV=1
Length = 624
Score = 125 bits (312), Expect = 7e-027
Identities = 56/100 (56%), Positives = 75/100 (75%)
Frame = -3
Query: 739 SPVRASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSR 560
+P ++ + I QK T SWV KG+TYYRYS V NKS +K+L LSI LYG +WGLS+
Sbjct: 524 APASSTADITIEQKETASWVPKGKTYYRYSVIVTNKSAMTMKNLKLSIYQLYGSLWGLSK 583
Query: 559 SGNSFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
G+S+ P+W++SL +GK+LEFVY+HS SPA V++SSYTL
Sbjct: 584 YGDSYVFPAWLNSLPAGKTLEFVYVHSASPATVSISSYTL 623
>tr|A6N4B9|A6N4B9_MANIN Endo-beta-1,4-glucanase OS=Mangifera indica PE=2 SV=1
Length = 619
Score = 103 bits (256), Expect = 2e-020
Identities = 50/95 (52%), Positives = 66/95 (69%), Gaps = 1/95 (1%)
Frame = -3
Query: 727 ASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSRSGNS 548
+S P+AI QKMT SW +KG+ YYRYST + NKS + LK LSI L+ + GL++ GN
Sbjct: 521 SSNPIAIEQKMTTSWDAKGQKYYRYSTIITNKSDKTLKDSQLSISALWSSV-GLNKVGNV 579
Query: 547 FGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYT 443
+G PSW+HSL +GK VYIH+ PA V+V SY+
Sbjct: 580 YGFPSWLHSLPAGKVWSLVYIHAADPAAVSVFSYS 614
>tr|A2WM56|A2WM56_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
indica GN=OsI_00928 PE=4 SV=1
Length = 640
Score = 96 bits (236), Expect = 4e-018
Identities = 46/99 (46%), Positives = 65/99 (65%), Gaps = 4/99 (4%)
Frame = -3
Query: 724 SGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSP---RPLKSLNLSIKNLYGPIWGLSRSG 554
+ PV I QK T SW GRTY+RY+ TV N+SP + ++ L++ I LYGP+WGL ++
Sbjct: 541 ASPVEIEQKATASWEKDGRTYHRYAVTVSNRSPAGGKTVEELHIGIGKLYGPVWGLEKAA 600
Query: 553 N-SFGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
+ LPSW SL +G+S FVY+H+ PA+V V+ Y L
Sbjct: 601 RYGYVLPSWTPSLPAGESAAFVYVHAAPPADVWVTGYKL 639
>tr|C5YUQ8|C5YUQ8_SORBI Putative uncharacterized protein Sb09g006670 OS=Sorghum
bicolor GN=Sb09g006670 PE=4 SV=1
Length = 627
Score = 92 bits (227), Expect = 5e-017
Identities = 41/96 (42%), Positives = 62/96 (64%)
Frame = -3
Query: 727 ASGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSRSGNS 548
+S P+ I Q +T +W + TYYRYS TV N+S + ++ L+L + L G +WGL R+
Sbjct: 531 SSSPIEIEQNVTRTWARRRTTYYRYSVTVTNRSRKTVRELHLGVSELRGRLWGLDRARYG 590
Query: 547 FGLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
+ P W+ +L++GKSL FVY+ +PANV V+ Y L
Sbjct: 591 YVPPKWLPALRAGKSLRFVYVQHGTPANVWVTGYKL 626
>tr|C0HF28|C0HF28_MAIZE Putative uncharacterized protein OS=Zea mays PE=2 SV=1
Length = 623
Score = 89 bits (219), Expect = 4e-016
Identities = 39/95 (41%), Positives = 60/95 (63%)
Frame = -3
Query: 724 SGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSRSGNSF 545
S P+ I Q +T +W + TYYRYS T+ N+S + ++ L+L + L G +WGL ++ +
Sbjct: 528 SSPIEIEQNVTRTWAMRRATYYRYSVTLTNRSRKTVRELHLGVSELRGRLWGLDKARYGY 587
Query: 544 GLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
P W+ +L++GKSL FVY+ +PANV V Y L
Sbjct: 588 VPPKWLPALRAGKSLRFVYVQHGTPANVRVIGYKL 622
>tr|C0P2V6|C0P2V6_MAIZE Putative uncharacterized protein OS=Zea mays PE=2 SV=1
Length = 561
Score = 89 bits (219), Expect = 4e-016
Identities = 39/95 (41%), Positives = 60/95 (63%)
Frame = -3
Query: 724 SGPVAIVQKMTGSWVSKGRTYYRYSTTVINKSPRPLKSLNLSIKNLYGPIWGLSRSGNSF 545
S P+ I Q +T +W + TYYRYS T+ N+S + ++ L+L + L G +WGL ++ +
Sbjct: 466 SSPIEIEQNVTRTWAMRRATYYRYSVTLTNRSRKTVRELHLGVSELRGRLWGLDKARYGY 525
Query: 544 GLPSWMHSLQSGKSLEFVYIHSTSPANVAVSSYTL 440
P W+ +L++GKSL FVY+ +PANV V Y L
Sbjct: 526 VPPKWLPALRAGKSLRFVYVQHGTPANVRVIGYKL 560
Database: UniProt/TrEMBL
Posted date: Sat Aug 07 14:51:12 2010
Number of letters in database: 3,661,877,547
Number of sequences in database: 11,397,958
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,191,382,888,655
Number of Sequences: 11397958
Number of Extensions: 4191382888655
Number of Successful Extensions: 1459862553
Number of sequences better than 0.0: 0
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