BLASTX 7.6.2
Query= UN65429 /QuerySize=680
(679 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P48622|FAD3D_ARATH Temperature-sensitive omega-3 fatty acid d... 291 3e-078
sp|P46310|FAD3C_ARATH Omega-3 fatty acid desaturase, chloroplast... 242 2e-063
sp|P48620|FAD3C_SESIN Omega-3 fatty acid desaturase, chloroplast... 231 4e-060
sp|P48618|FAD3C_BRANA Omega-3 fatty acid desaturase, chloroplast... 203 1e-051
sp|P48621|FAD3C_SOYBN Omega-3 fatty acid desaturase, chloroplast... 178 5e-044
sp|P48619|FAD3C_RICCO Omega-3 fatty acid desaturase, chloroplast... 176 1e-043
sp|P32291|FAD3E_PHAAU Omega-3 fatty acid desaturase, endoplasmic... 151 4e-036
sp|P48623|FAD3E_ARATH Omega-3 fatty acid desaturase, endoplasmic... 147 7e-035
sp|P48624|FAD3E_BRANA Omega-3 fatty acid desaturase, endoplasmic... 146 2e-034
sp|P48625|FAD3E_SOYBN Omega-3 fatty acid desaturase, endoplasmic... 144 8e-034
sp|P48626|FAD3E_TOBAC Omega-3 fatty acid desaturase, endoplasmic... 137 7e-032
sp|Q54794|DESA_SPIPL Fatty acid desaturase OS=Spirulina platensi... 53 2e-006
sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplast... 50 9e-006
>sp|P48622|FAD3D_ARATH Temperature-sensitive omega-3 fatty acid desaturase,
chloroplastic OS=Arabidopsis thaliana GN=FAD8 PE=2 SV=1
Length = 435
Score = 291 bits (744), Expect = 3e-078
Identities = 138/168 (82%), Positives = 143/168 (85%), Gaps = 3/168 (1%)
Frame = +3
Query: 177 MASCVLSECGIRPLPRFYPKPTNSFPSNPSPTLKLNPPVTPLPS-LNTRSWIFPRTRNWA 353
MAS VLSECG RPLPRFYPK T SF SNP PT K NPP+ P S LN+R + +TRNWA
Sbjct: 1 MASSVLSECGFRPLPRFYPKHTTSFASNPKPTFKFNPPLKPPSSLLNSRYGFYSKTRNWA 60
Query: 354 LNVATPLTTLETQPEEEEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDV 533
LNVATPLTTL Q EED RFDPGAPPPFNLADIRAAIPKHCWVKNPW SM YVVRDV
Sbjct: 61 LNVATPLTTL--QSPSEEDTERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDV 118
Query: 534 AIVFGLAAVAAYFNSWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 677
AIVFGLAAVAAYFN+WLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND
Sbjct: 119 AIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 166
>sp|P46310|FAD3C_ARATH Omega-3 fatty acid desaturase, chloroplastic
OS=Arabidopsis thaliana GN=FAD7 PE=1 SV=1
Length = 446
Score = 242 bits (616), Expect = 2e-063
Identities = 122/176 (69%), Positives = 137/176 (77%), Gaps = 12/176 (6%)
Frame = +3
Query: 177 MASCVLSECGIRPLPRFYPKPTNSFPSNPS---PTLKLNPPVT---PLP-SLNTRSWIFP 335
MA+ VLSECGIRPLPR Y P ++F SN + P+L + T PL LN+R
Sbjct: 1 MANLVLSECGIRPLPRIYTTPRSNFLSNNNKFRPSLSSSSYKTSSSPLSFGLNSRDGF-- 58
Query: 336 RTRNWALNVATPLTT--LETQPEEEEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKS 509
TRNWALNV+TPLTT E P EE++ +RFDPGAPPPFNLADIRAAIPKHCWVKNPWKS
Sbjct: 59 -TRNWALNVSTPLTTPIFEESPLEEDNKQRFDPGAPPPFNLADIRAAIPKHCWVKNPWKS 117
Query: 510 MGYVVRDVAIVFGLAAVAAYFNSWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 677
+ YVVRDVAIVF LAA AAY N+W++WPLYW AQGTMFWALFVLGHDCGHGSFSND
Sbjct: 118 LSYVVRDVAIVFALAAGAAYLNNWIVWPLYWLAQGTMFWALFVLGHDCGHGSFSND 173
>sp|P48620|FAD3C_SESIN Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum
indicum GN=FAD7 PE=2 SV=1
Length = 447
Score = 231 bits (588), Expect = 4e-060
Identities = 115/178 (64%), Positives = 127/178 (71%), Gaps = 12/178 (6%)
Frame = +3
Query: 177 MASCVLSECGIRPLPRFYPKPTNSFPSNPSPTLKLNPPVTPL---PSLNTRSWIFPRTRN 347
MAS VLSECG+RPLPR YPKP P S KL T L S S I R +N
Sbjct: 1 MASWVLSECGLRPLPRVYPKPRTGHPLLNSNPTKLRFSRTDLGNGSSFCLSSGIL-REKN 59
Query: 348 WALNVATPLTTLETQPEEE--------EDARRFDPGAPPPFNLADIRAAIPKHCWVKNPW 503
WAL V+ PL L+ + EEE FDPGAPPPF L+DIR AIPKHCWVK+PW
Sbjct: 60 WALRVSAPLRVLQVEEEEENKEGERVINGGEEFDPGAPPPFKLSDIREAIPKHCWVKDPW 119
Query: 504 KSMGYVVRDVAIVFGLAAVAAYFNSWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 677
+SMGYVVRDVA+VFGLAAVAAYFN+W++WPLYWFAQ TMFWALFVLGHDCGHGSFSND
Sbjct: 120 RSMGYVVRDVAVVFGLAAVAAYFNNWVVWPLYWFAQSTMFWALFVLGHDCGHGSFSND 177
>sp|P48618|FAD3C_BRANA Omega-3 fatty acid desaturase, chloroplastic (Fragment)
OS=Brassica napus GN=FAD7 PE=2 SV=1
Length = 404
Score = 203 bits (514), Expect = 1e-051
Identities = 92/116 (79%), Positives = 99/116 (85%), Gaps = 3/116 (2%)
Frame = +3
Query: 339 TRNWALNVATPLTTLETQP---EEEEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKS 509
+RNWALNV TPLT + EEE +RFDPGAPPPFNLADIRAAIPKHCWVKNPWKS
Sbjct: 16 SRNWALNVTTPLTVDSSSSPPIEEEPKTQRFDPGAPPPFNLADIRAAIPKHCWVKNPWKS 75
Query: 510 MGYVVRDVAIVFGLAAVAAYFNSWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 677
M YVVR++AIVF LAA AAY N+WL+WPLYW AQGTMFWALFVLGHDCGHGSFSND
Sbjct: 76 MSYVVRELAIVFALAAGAAYLNNWLVWPLYWIAQGTMFWALFVLGHDCGHGSFSND 131
>sp|P48621|FAD3C_SOYBN Omega-3 fatty acid desaturase, chloroplastic OS=Glycine
max GN=FAD7 PE=2 SV=1
Length = 453
Score = 178 bits (449), Expect = 5e-044
Identities = 78/97 (80%), Positives = 83/97 (85%)
Frame = +3
Query: 387 TQPEEEEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAA 566
T E E FDPGAPPPFNLADIRAAIPKHCWVK+PW+SM YVVRDV VFGLAA AA
Sbjct: 85 TNGVEHEKLPEFDPGAPPPFNLADIRAAIPKHCWVKDPWRSMSYVVRDVIAVFGLAAAAA 144
Query: 567 YFNSWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 677
Y N+WL+WPLYW AQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 145 YLNNWLVWPLYWAAQGTMFWALFVLGHDCGHGSFSNN 181
>sp|P48619|FAD3C_RICCO Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus
communis GN=FAD7A-1 PE=2 SV=1
Length = 460
Score = 176 bits (445), Expect = 1e-043
Identities = 75/93 (80%), Positives = 82/93 (88%)
Frame = +3
Query: 399 EEEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNS 578
+E FD GAPPPF LADIRAAIPKHCWVKNPW+SM YV+RDV +VFGLAAVAAYFN+
Sbjct: 95 DEGKGEFFDAGAPPPFTLADIRAAIPKHCWVKNPWRSMSYVLRDVVVVFGLAAVAAYFNN 154
Query: 579 WLLWPLYWFAQGTMFWALFVLGHDCGHGSFSND 677
W+ WPLYWF QGTMFWALFVLGHDCGHGSFSN+
Sbjct: 155 WVAWPLYWFCQGTMFWALFVLGHDCGHGSFSNN 187
Score = 59 bits (142), Expect = 2e-008
Identities = 32/87 (36%), Positives = 42/87 (48%), Gaps = 5/87 (5%)
Frame = +3
Query: 180 ASCVLSECGIRPLPRFYPKPTNSFPSNPSPTLKL-----NPPVTPLPSLNTRSWIFPRTR 344
A VLSECG+RPLPR Y +P F S + LKL + S SW +
Sbjct: 3 AGWVLSECGLRPLPRIYSRPRIGFTSKTTNLLKLRELPDSKSYNLCSSFKVSSWSNSKQS 62
Query: 345 NWALNVATPLTTLETQPEEEEDARRFD 425
NWALNVA P+ E++ + F+
Sbjct: 63 NWALNVAVPVNVSTVSGEDDREREEFN 89
>sp|P32291|FAD3E_PHAAU Omega-3 fatty acid desaturase, endoplasmic reticulum
OS=Phaseolus aureus GN=ARG1 PE=2 SV=1
Length = 380
Score = 151 bits (381), Expect = 4e-036
Identities = 67/91 (73%), Positives = 73/91 (80%)
Frame = +3
Query: 402 EEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNSW 581
E D FDPGAPPPF +ADIRAAIPKHCW K+ +S+ YV+RDV +V LAA A FNSW
Sbjct: 16 EGDQSYFDPGAPPPFKIADIRAAIPKHCWEKSTLRSLSYVLRDVLVVTALAASAISFNSW 75
Query: 582 LLWPLYWFAQGTMFWALFVLGHDCGHGSFSN 674
WPLYW AQGTMFWALFVLGHDCGHGSFSN
Sbjct: 76 FFWPLYWPAQGTMFWALFVLGHDCGHGSFSN 106
>sp|P48623|FAD3E_ARATH Omega-3 fatty acid desaturase, endoplasmic reticulum
OS=Arabidopsis thaliana GN=FAD3 PE=2 SV=1
Length = 386
Score = 147 bits (370), Expect = 7e-035
Identities = 62/92 (67%), Positives = 72/92 (78%)
Frame = +3
Query: 399 EEEDARRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNS 578
+ + RFDP A PPF + DIRAAIPKHCWVK+P +SM YVVRD+ V LA A Y +S
Sbjct: 19 DRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDS 78
Query: 579 WLLWPLYWFAQGTMFWALFVLGHDCGHGSFSN 674
W LWPLYW AQGT+FWA+FVLGHDCGHGSFS+
Sbjct: 79 WFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSD 110
>sp|P48624|FAD3E_BRANA Omega-3 fatty acid desaturase, endoplasmic reticulum
OS=Brassica napus GN=FAD3 PE=2 SV=1
Length = 383
Score = 146 bits (367), Expect = 2e-034
Identities = 61/85 (71%), Positives = 69/85 (81%)
Frame = +3
Query: 420 FDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNSWLLWPLY 599
FDP A PPF + DIRAAIPKHCWVK+P +SM YV RD+ V LA A YF+SW LWPLY
Sbjct: 23 FDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVTRDIFAVAALAMAAVYFDSWFLWPLY 82
Query: 600 WFAQGTMFWALFVLGHDCGHGSFSN 674
W AQGT+FWA+FVLGHDCGHGSFS+
Sbjct: 83 WVAQGTLFWAIFVLGHDCGHGSFSD 107
>sp|P48625|FAD3E_SOYBN Omega-3 fatty acid desaturase, endoplasmic reticulum
OS=Glycine max GN=FAD3 PE=2 SV=1
Length = 380
Score = 144 bits (361), Expect = 8e-034
Identities = 59/85 (69%), Positives = 72/85 (84%)
Frame = +3
Query: 420 FDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNSWLLWPLY 599
FDP APPPF +A+IRA+IPKHCWVKNPW+S+ YV+RDV ++ L A A +F++WLLW +Y
Sbjct: 25 FDPSAPPPFKIAEIRASIPKHCWVKNPWRSLSYVLRDVLVIAALVAAAIHFDNWLLWLIY 84
Query: 600 WFAQGTMFWALFVLGHDCGHGSFSN 674
QGTMFWALFVLGHDCGHGSFS+
Sbjct: 85 CPIQGTMFWALFVLGHDCGHGSFSD 109
>sp|P48626|FAD3E_TOBAC Omega-3 fatty acid desaturase, endoplasmic reticulum
OS=Nicotiana tabacum GN=FAD3 PE=2 SV=1
Length = 379
Score = 137 bits (344), Expect = 7e-032
Identities = 58/85 (68%), Positives = 68/85 (80%)
Frame = +3
Query: 420 FDPGAPPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNSWLLWPLY 599
FDP APPPF LA+IR IPKHCWVK+P +S+ YVVRDV V L +A + +SWL +PLY
Sbjct: 22 FDPSAPPPFRLAEIRNVIPKHCWVKDPLRSLSYVVRDVIFVATLIGIAIHLDSWLFYPLY 81
Query: 600 WFAQGTMFWALFVLGHDCGHGSFSN 674
W QGTMFWA+FVLGHDCGHGSFS+
Sbjct: 82 WAIQGTMFWAIFVLGHDCGHGSFSD 106
>sp|Q54794|DESA_SPIPL Fatty acid desaturase OS=Spirulina platensis GN=desA PE=3
SV=1
Length = 351
Score = 53 bits (125), Expect = 2e-006
Identities = 27/74 (36%), Positives = 40/74 (54%), Gaps = 1/74 (1%)
Frame = +3
Query: 456 DIRAAIPKHCWVKNPWKSMGYVVRDVAIVFGLAAVAAYFNSWLLWPLYWFAQGTMFWALF 635
DI +P +V++ +K+ V+ +V +V GL + W L P+ W GT F
Sbjct: 27 DIINTLPSGVFVQDRYKAWMTVIINVVMV-GLGWLGIAIAPWFLLPVVWVFTGTALTGFF 85
Query: 636 VLGHDCGHGSFSND 677
V+GHDCGH SFS +
Sbjct: 86 VIGHDCGHRSFSRN 99
>sp|P48629|FAD6C_SPIOL Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia
oleracea GN=FAD6 PE=1 SV=1
Length = 447
Score = 50 bits (119), Expect = 9e-006
Identities = 29/82 (35%), Positives = 40/82 (48%), Gaps = 3/82 (3%)
Frame = +3
Query: 435 PPPFNLADIRAAIPKHCWVKNPWKSMGYVVRDV-AIVFGLAAVAAYFNSWLLWPLYWFAQ 611
P + DI ++PK + N K+ G V+ V + G+ +A W L PL W
Sbjct: 102 PDDVTMRDIITSLPKQVFEINDTKAWGTVLISVTSYALGIFMIAK--APWYLLPLAWAWT 159
Query: 612 GTMFWALFVLGHDCGHGSFSND 677
GT FV+GHDC H SFS +
Sbjct: 160 GTAITGFFVIGHDCAHKSFSKN 181
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 224,056,207,302
Number of Sequences: 518415
Number of Extensions: 224056207302
Number of Successful Extensions: 1407786191
Number of sequences better than 0.0: 0
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