BLASTX 7.6.2
Query= UN72509 /QuerySize=749
(748 letters)
Database: UniProt/SwissProt;
518,415 sequences; 182,829,261 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5EAR5|TRPT1_DANRE tRNA 2'-phosphotransferase 1 OS=Danio reri... 111 5e-024
sp|Q8K3A2|TRPT1_MOUSE tRNA 2'-phosphotransferase 1 OS=Mus muscul... 105 5e-022
sp|Q3ZBM7|TRPT1_BOVIN tRNA 2'-phosphotransferase 1 OS=Bos taurus... 95 4e-019
sp|Q86TN4|TRPT1_HUMAN tRNA 2'-phosphotransferase 1 OS=Homo sapie... 93 2e-018
sp|O14045|TPT1_SCHPO Putative tRNA 2'-phosphotransferase OS=Schi... 87 1e-016
sp|Q0TUT1|KPTA_CLOP1 Probable RNA 2'-phosphotransferase OS=Clost... 73 2e-012
sp|Q8XP22|KPTA_CLOPE Probable RNA 2'-phosphotransferase OS=Clost... 73 2e-012
sp|Q8R5N7|KPTA_FUSNN Probable RNA 2'-phosphotransferase OS=Fusob... 64 1e-009
sp|B6YXF3|KPTA_THEON Probable RNA 2'-phosphotransferase OS=Therm... 56 3e-007
>sp|Q5EAR5|TRPT1_DANRE tRNA 2'-phosphotransferase 1 OS=Danio rerio GN=trpt1 PE=2
SV=2
Length = 225
Score = 111 bits (277), Expect = 5e-024
Identities = 71/173 (41%), Positives = 103/173 (59%), Gaps = 22/173 (12%)
Frame = +2
Query: 188 RGDGRGGGGGKDKID---ALGRLLTRILRHMATELRLNMRGDGFVKVEDLLQLNLKTSAN 358
RG GR G G +++ L + L+ +LRH A+++ L M DGFV VE+LL A+
Sbjct: 6 RGRGRRGRGNRNEESRDVRLSKSLSYVLRHGASKMGLQMNSDGFVFVEELL-------AH 58
Query: 359 IQLKSHTIDEIREAVRRDNKQRFSVVE--ENGELFIRANQGHSITTVESEKLLKPILS-- 526
Q +S ++D++ V ++KQRF + + E+ L IRANQGHS+ + E L+ I
Sbjct: 59 QQFRSFSVDDVERVVASNDKQRFKLCKHPEDDRLQIRANQGHSVQVTDLE--LREISQDD 116
Query: 527 ---PEEAPVCVHGTYRKNLESILASGLKRMDRLHVHFSCGLPTDGEVISGMRR 676
P EA VHG+Y K+ SI + GL RM+R H+H + GLP +G VISGMR+
Sbjct: 117 QDYPREA---VHGSYMKHWPSIRSQGLSRMNRTHIHLAPGLPGEGRVISGMRQ 166
>sp|Q8K3A2|TRPT1_MOUSE tRNA 2'-phosphotransferase 1 OS=Mus musculus GN=Trpt1
PE=2 SV=2
Length = 249
Score = 105 bits (260), Expect = 5e-022
Identities = 71/170 (41%), Positives = 98/170 (57%), Gaps = 14/170 (8%)
Frame = +2
Query: 173 RRPQGRGDGRGGGGGKDKIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLQLNLKTS 352
RR +GR R +D+ L + L+ LRH A +L L MR DGFV ++ LLQL
Sbjct: 7 RRKEGRRTHR--PREQDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLP---- 60
Query: 353 ANIQLKSHTIDEIREAVRRDNKQRFSVV--EENGELFIRANQGHSITTVESEKLLKPILS 526
Q S +I++++ V + KQRF++ E + L IRANQGHS+ E E L P+ +
Sbjct: 61 ---QFHSFSIEDVQLVVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELE--LTPLET 115
Query: 527 PEEAPV-CVHGTYRKNLESILASGLKRMDRLHVHFSCGLPTDGEVISGMR 673
P+ P+ VHGT+ K+ SIL GL R R H+H + GLP D VISG+R
Sbjct: 116 PQALPLTLVHGTFWKHWPSILLKGLSRQGRTHIHLASGLPGDPGVISGIR 165
>sp|Q3ZBM7|TRPT1_BOVIN tRNA 2'-phosphotransferase 1 OS=Bos taurus GN=TRPT1 PE=2
SV=3
Length = 254
Score = 95 bits (235), Expect = 4e-019
Identities = 62/155 (40%), Positives = 91/155 (58%), Gaps = 12/155 (7%)
Frame = +2
Query: 218 KDKIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLQLNLKTSANIQLKSHTIDEIRE 397
+D+ L + L+ LRH A +L L M DGFV ++ LLQL Q +S + ++++
Sbjct: 26 QDRDVQLSKALSYALRHGALKLGLPMGADGFVPLDALLQLP-------QFRSFSAEDVQR 78
Query: 398 AVRRDNKQRFSVV--EENGELFIRANQGHSITTVESEKLLKPILSPEEAP-VCVHGTYRK 568
V + KQRF++ + + IRANQGHS+ E E L+P+ +P+ P + VHGT+R+
Sbjct: 79 VVDTNVKQRFALQPGDPSTGPLIRANQGHSLQVPELE--LEPLETPQALPLMLVHGTFRQ 136
Query: 569 NLESILASGLKRMDRLHVHFSCGLPTDGEVISGMR 673
+ SIL GL R H+H + GLP D VISGMR
Sbjct: 137 HWPSILLKGLSCRGRTHIHLAPGLPGDPGVISGMR 171
>sp|Q86TN4|TRPT1_HUMAN tRNA 2'-phosphotransferase 1 OS=Homo sapiens GN=TRPT1
PE=1 SV=2
Length = 253
Score = 93 bits (229), Expect = 2e-018
Identities = 64/173 (36%), Positives = 93/173 (53%), Gaps = 19/173 (10%)
Frame = +2
Query: 185 GRGDGRGGGGGK-------DKIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLQLNL 343
GR + G G + D+ L + L+ LRH A +L L M DGFV + LLQL
Sbjct: 7 GRQEAAGSRGRRAPRPREQDRDVQLSKALSYALRHGALKLGLPMGADGFVPLGTLLQLP- 65
Query: 344 KTSANIQLKSHTIDEIREAVRRDNKQRFSVV--EENGELFIRANQGHSITTVESEKLLKP 517
Q + + ++++ V + KQRF++ + + L IRANQGHS+ + E L P
Sbjct: 66 ------QFRGFSAEDVQRVVDTNRKQRFALQLGDPSTGLLIRANQGHSLQVPKLE--LMP 117
Query: 518 ILSPEE-APVCVHGTYRKNLESILASGLKRMDRLHVHFSCGLPTDGEVISGMR 673
+ +P+ P+ VHGT+ K+ SIL GL R H+H + GLP D +ISGMR
Sbjct: 118 LETPQALPPMLVHGTFWKHWPSILLKGLSCQGRTHIHLAPGLPGDPGIISGMR 170
>sp|O14045|TPT1_SCHPO Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces
pombe GN=SPAC2C4.12c PE=2 SV=1
Length = 361
Score = 87 bits (214), Expect = 1e-016
Identities = 55/163 (33%), Positives = 90/163 (55%), Gaps = 9/163 (5%)
Frame = +2
Query: 242 RLLTRILRHMATELRLNMRGDGFVKVEDLLQLNLKTSANIQLKSHTIDEIREAVRRDNKQ 421
+ L+++LRH A L +R DG+++V+ +L+L Q + ++ + V+ ++K+
Sbjct: 39 KALSKVLRHTAKANGLQIREDGYIEVDSILKLP-------QFRGMGMELLLSIVKGNDKK 91
Query: 422 RFSVVEENGELFIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLESILASGLK 601
RF++ E G L+IRANQGHSI V+ + I + P VHGT ++ I GL
Sbjct: 92 RFTMEEVEGVLYIRANQGHSIKAVQVP--MARIDNASSIPKVVHGTKKELWPVISKQGLS 149
Query: 602 RMDRLHVHFSCGLPTDGEVISGMRRMLMS*SSLTSRKLLKMGL 730
RM R H+H + GL D VISG+R+ + S K ++ G+
Sbjct: 150 RMKRNHIHCATGLYGDPGVISGIRKSCTLYIYIDSAKAMQDGV 192
>sp|Q0TUT1|KPTA_CLOP1 Probable RNA 2'-phosphotransferase OS=Clostridium
perfringens (strain ATCC 13124 / NCTC 8237 / Type A) GN=kptA PE=3 SV=1
Length = 186
Score = 73 bits (178), Expect = 2e-012
Identities = 50/130 (38%), Positives = 74/130 (56%), Gaps = 13/130 (10%)
Frame = +2
Query: 257 ILRHMATELRLNMRGDGFVKVEDLLQLNLKTSANIQLKSHTIDEIREAVRRDNKQRFSVV 436
ILRH E+ L + G++ V DL++ N + ++D++ VR D+K R+S
Sbjct: 15 ILRHKPEEIGLKLDEHGYLGVLDLIE-----GLNKSYEGFSMDDLERIVREDSKGRYSFN 69
Query: 437 EENGELFIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLESILASGLKRMDRL 616
E+ + IRANQGHSI + + L+PI P+ V HGT RK LESIL +GL + +R
Sbjct: 70 EDKSK--IRANQGHSI---KVDLGLEPIKPPK---VLYHGTGRKYLESILKNGLIKKERN 121
Query: 617 HVHFSCGLPT 646
+VH S + T
Sbjct: 122 YVHLSKDIET 131
>sp|Q8XP22|KPTA_CLOPE Probable RNA 2'-phosphotransferase OS=Clostridium
perfringens GN=kptA PE=3 SV=1
Length = 183
Score = 73 bits (178), Expect = 2e-012
Identities = 48/130 (36%), Positives = 70/130 (53%), Gaps = 13/130 (10%)
Frame = +2
Query: 257 ILRHMATELRLNMRGDGFVKVEDLLQLNLKTSANIQLKSHTIDEIREAVRRDNKQRFSVV 436
ILRH E+ L + G++ V DL++ N K ++D++ VR D+K R+S
Sbjct: 15 ILRHKPEEIGLKLDEHGYLGVLDLIE-----GLNKSYKGFSMDDLERIVREDSKGRYSFN 69
Query: 437 EENGELFIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLESILASGLKRMDRL 616
E+ + IRANQGHSI + +KP V HGT RK +ESIL +GL + +R
Sbjct: 70 EDKSK--IRANQGHSIKVDLGLEAIKP------PKVLYHGTGRKYIESILKNGLIKKERQ 121
Query: 617 HVHFSCGLPT 646
+VH S + T
Sbjct: 122 YVHLSKDIET 131
>sp|Q8R5N7|KPTA_FUSNN Probable RNA 2'-phosphotransferase OS=Fusobacterium
nucleatum subsp. nucleatum GN=kptA PE=3 SV=1
Length = 179
Score = 64 bits (154), Expect = 1e-009
Identities = 44/130 (33%), Positives = 66/130 (50%), Gaps = 13/130 (10%)
Frame = +2
Query: 257 ILRHMATELRLNMRGDGFVKVEDLLQLNLKTSANIQLKSHTIDEIREAVRRDNKQRFSVV 436
ILRH + L + +G+ ++L++ K+ I + + V +NK+R+S
Sbjct: 14 ILRHKPETINLKLDKNGWANTKELIEKISKSGREID-----FEILERIVNENNKKRYSFN 68
Query: 437 EENGELFIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLESILASGLKRMDRL 616
E+ + IRA QGHSI E LK ++ P + HGT K LESI G+K+M R
Sbjct: 69 EDKTK--IRAVQGHSI---EVNLELKEVVPP---AILYHGTAFKTLESIKKEGIKKMSRQ 120
Query: 617 HVHFSCGLPT 646
HVH S + T
Sbjct: 121 HVHLSADIET 130
>sp|B6YXF3|KPTA_THEON Probable RNA 2'-phosphotransferase OS=Thermococcus
onnurineus (strain NA1) GN=kptA PE=3 SV=1
Length = 180
Score = 56 bits (133), Expect = 3e-007
Identities = 46/125 (36%), Positives = 62/125 (49%), Gaps = 17/125 (13%)
Frame = +2
Query: 260 LRHMATELRLNMRGDGFVKVEDLLQLNLKTSANIQLKSHTIDEIREAVRRDNKQRFSVVE 439
LRH E L +GFV + +L++ LKT T + +RE V RD K R+ +
Sbjct: 16 LRHSPEEFGLRPDVEGFVPLSELVEA-LKTI----YPDVTEEFVREIVARDAKGRYEIQG 70
Query: 440 ENGELFIRANQGHSI-TTVESEKLLKPILSPEEAPVCVHGTYRKNLESILASGLKRMDRL 616
+ IRA GHS +++ E+ E+ HGT R+NLESIL GLK M R
Sbjct: 71 DR----IRARYGHSFPVSLDHEE-------DTESRFLYHGTPRRNLESILREGLKPMRRQ 119
Query: 617 HVHFS 631
VH S
Sbjct: 120 FVHLS 124
Database: UniProt/SwissProt
Posted date: Sat Aug 07 14:36:18 2010
Number of letters in database: 182,829,261
Number of sequences in database: 518,415
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 239,089,486,026
Number of Sequences: 518415
Number of Extensions: 239089486026
Number of Successful Extensions: 1518758948
Number of sequences better than 0.0: 0
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