BLASTX 7.6.2
Query= RU13269 /QuerySize=953
(952 letters)
Database: GenBank nr;
7,387,702 sequences; 2,551,671,255 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|83778968|gb|ABB86962.1| cytosolic NADP-malic enzyme [Malus x ... 431 5e-119
gi|2150027|gb|AAB58727.1| NADP-malic enzyme [Lycopersicon escule... 368 4e-100
gi|15219980|ref|NP_178093.1| ATNADP-ME4 (NADP-MALIC ENZYME 4); m... 366 3e-099
gi|8118507|gb|AAF73006.1|AF262997_1 NADP-dependent malic protein... 365 4e-099
gi|2911148|dbj|BAA24950.1| NADP-malic enzyme [Aloe arborescens] 348 4e-094
>gi|83778968|gb|ABB86962.1| cytosolic NADP-malic enzyme [Malus x domestica]
Length = 641
Score = 431 bits (1107), Expect = 5e-119
Identities = 220/268 (82%), Positives = 239/268 (89%), Gaps = 7/268 (2%)
Frame = +3
Query: 165 MISLNRSCFLSNGGISGNCSPFSQGQKRRSASLKVVAVGTNAARKGERNGSIVMENAAK- 341
M+SLNRSCFL N ++G+ SP SQ QKR+ ASLKVVA+ AR G+RNGS+VME+ +
Sbjct: 1 MMSLNRSCFLGNPAVAGSSSPSSQSQKRQPASLKVVAL-IPKARTGDRNGSVVMESTLQE 59
Query: 342 -----EVVELKSTVHGGDKDVYGEDTASEDQPVTPWSVSVASGYNLIRDPHHNKGLAFSE 506
EVVELKSTVHGG +DVYGEDTA+EDQ VTPWSVSVASGY LIR PHHNKGLAF+E
Sbjct: 60 VKTESEVVELKSTVHGGVEDVYGEDTATEDQLVTPWSVSVASGYMLIRSPHHNKGLAFTE 119
Query: 507 KERDAHYMRGLLPPVVISQELQVKKMINAIRQYQVPLQKYIAMMDLQERNQKLFYKLLIE 686
KERDAHY+RGLLPPVVISQELQVKKMIN+IRQYQVPLQKYIAMMDLQ RN+KLFYKLLIE
Sbjct: 120 KERDAHYLRGLLPPVVISQELQVKKMINSIRQYQVPLQKYIAMMDLQGRNEKLFYKLLIE 179
Query: 687 HVEELLPVVYTPTVGEACQKYGSIFQRPQGLFISLKEKGKILEVLRNWPEKNIQVIVVTD 866
H EELLPVVYTPTVGEACQKYGSIF +PQGLFISLKEKGKILEV RNWPE+NIQVIVVTD
Sbjct: 180 HAEELLPVVYTPTVGEACQKYGSIFTQPQGLFISLKEKGKILEVXRNWPEQNIQVIVVTD 239
Query: 867 GERILGLGDLGCHGMGIPVGKLSLYTAL 950
GERILGLGDLG HGMGIPVGKL+LYTAL
Sbjct: 240 GERILGLGDLGSHGMGIPVGKLALYTAL 267
>gi|2150027|gb|AAB58727.1| NADP-malic enzyme [Lycopersicon esculentum]
Length = 640
Score = 368 bits (944), Expect = 4e-100
Identities = 184/267 (68%), Positives = 225/267 (84%), Gaps = 6/267 (2%)
Frame = +3
Query: 165 MISLNRSCFLSNGGISGNCSPFSQGQKRRSASLKVVAVGTNAARKGERNGSIVMENAAKE 344
M SLN + F +N +SG +Q +R SA + VVA + + G+ + S+++ENA E
Sbjct: 1 MFSLNGTSF-TNNSLSGVSRCLNQSSRRVSAPMVVVAAVNSNGKPGDGSVSVLVENALTE 59
Query: 345 -----VVELKSTVHGGDKDVYGEDTASEDQPVTPWSVSVASGYNLIRDPHHNKGLAFSEK 509
+E+KSTV GG +DVYGED+A+EDQ +TPW++SVASG++L+R+PH+NKGLAFSE+
Sbjct: 60 SPAPVEMEVKSTVTGGVQDVYGEDSATEDQSITPWTLSVASGFSLLRNPHYNKGLAFSER 119
Query: 510 ERDAHYMRGLLPPVVISQELQVKKMINAIRQYQVPLQKYIAMMDLQERNQKLFYKLLIEH 689
ERD HY+RGLLPPVVIS +LQVKKM+N+IR+Y VPLQ+Y+AMMDLQE N++LFYKLLI++
Sbjct: 120 ERDTHYLRGLLPPVVISHDLQVKKMMNSIRKYDVPLQRYMAMMDLQEMNERLFYKLLIDN 179
Query: 690 VEELLPVVYTPTVGEACQKYGSIFQRPQGLFISLKEKGKILEVLRNWPEKNIQVIVVTDG 869
VEELLP+VYTPTVGEACQKYGSIF+RPQGLFISLKEKGKI EVL+NWPEK IQVIVVTDG
Sbjct: 180 VEELLPIVYTPTVGEACQKYGSIFRRPQGLFISLKEKGKIHEVLKNWPEKKIQVIVVTDG 239
Query: 870 ERILGLGDLGCHGMGIPVGKLSLYTAL 950
ERILGLGDLGC GMGIPVGKLSLYTAL
Sbjct: 240 ERILGLGDLGCQGMGIPVGKLSLYTAL 266
>gi|15219980|ref|NP_178093.1| ATNADP-ME4 (NADP-MALIC ENZYME 4); malate
dehydrogenase (oxaloacetate-decarboxylating) (NADP+)/ malic enzyme/
oxidoreductase, acting on NADH or NADPH, NAD or NADP as acceptor
[Arabidopsis thaliana]
Length = 646
Score = 366 bits (937), Expect = 3e-099
Identities = 191/273 (69%), Positives = 219/273 (80%), Gaps = 12/273 (4%)
Frame = +3
Query: 165 MISLNRSCFLSNGGISGNCSPFSQGQKRR----SASLKVV-AVGTNAARKGE------RN 311
MISL S FL+ + G + S Q R ASL+V ++G+N G
Sbjct: 1 MISLTPSLFLNKTVVPGCSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIETTAT 60
Query: 312 GSIVMENAAKEVVELKSTVHGGDKDVYGEDTASEDQPVTPWSVSVASGYNLIRDPHHNKG 491
S +E +A ++V KSTV GG +DVYGED A+ED P+TPWS+SVASGY L+RDPHHNKG
Sbjct: 61 SSSSLETSAADIVP-KSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKG 119
Query: 492 LAFSEKERDAHYMRGLLPPVVISQELQVKKMINAIRQYQVPLQKYIAMMDLQERNQKLFY 671
LAFS +ERDAHY+RGLLPP VISQ+LQVKK+++ +RQYQVPLQKY+AMMDLQE N++LFY
Sbjct: 120 LAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFY 179
Query: 672 KLLIEHVEELLPVVYTPTVGEACQKYGSIFQRPQGLFISLKEKGKILEVLRNWPEKNIQV 851
KLLI+HVEELLPV+YTPTVGEACQKYGSIF RPQGLFISLKEKGKI EVLRNWPEKNIQV
Sbjct: 180 KLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQV 239
Query: 852 IVVTDGERILGLGDLGCHGMGIPVGKLSLYTAL 950
IVVTDGERILGLGDLGC GMGIPVGKLSLYTAL
Sbjct: 240 IVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 272
>gi|8118507|gb|AAF73006.1|AF262997_1 NADP-dependent malic protein [Ricinus
communis]
Length = 641
Score = 365 bits (935), Expect = 4e-099
Identities = 180/236 (76%), Positives = 209/236 (88%), Gaps = 4/236 (1%)
Frame = +3
Query: 255 ASLKVVAVGTNAARKG----ERNGSIVMENAAKEVVELKSTVHGGDKDVYGEDTASEDQP 422
+SLKVVA+ N R G E + + A+ ++ KSTV GG +DVYGEDTA+EDQ
Sbjct: 32 SSLKVVALNPNRERNGSIMMESTLQEMRDGASVLDLDPKSTVAGGVRDVYGEDTATEDQF 91
Query: 423 VTPWSVSVASGYNLIRDPHHNKGLAFSEKERDAHYMRGLLPPVVISQELQVKKMINAIRQ 602
VTPWS+SVASGY+L+RDPHHNKGLAF++KERDAHY+RGLLPP ++SQELQVKKM++ IRQ
Sbjct: 92 VTPWSLSVASGYSLLRDPHHNKGLAFNDKERDAHYLRGLLPPAIVSQELQVKKMMHIIRQ 151
Query: 603 YQVPLQKYIAMMDLQERNQKLFYKLLIEHVEELLPVVYTPTVGEACQKYGSIFQRPQGLF 782
YQ+PLQKY+AMMDLQERN++LFYKLLI++VEE+LP+VYTPTVGEACQKYGSIF RPQGL+
Sbjct: 152 YQLPLQKYMAMMDLQERNERLFYKLLIQNVEEMLPIVYTPTVGEACQKYGSIFGRPQGLY 211
Query: 783 ISLKEKGKILEVLRNWPEKNIQVIVVTDGERILGLGDLGCHGMGIPVGKLSLYTAL 950
ISLKEKG+ILEVLRNWPEKNIQVIVVTDGERILGLGDLGC GMGIPVGKLSLYTAL
Sbjct: 212 ISLKEKGRILEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 267
>gi|2911148|dbj|BAA24950.1| NADP-malic enzyme [Aloe arborescens]
Length = 592
Score = 348 bits (892), Expect = 4e-094
Identities = 166/198 (83%), Positives = 186/198 (93%)
Frame = +3
Query: 357 KSTVHGGDKDVYGEDTASEDQPVTPWSVSVASGYNLIRDPHHNKGLAFSEKERDAHYMRG 536
K+ V GG DVYGED A+E+Q VTPW+VSVASGY+L+RDPHHNKGLAFSEKERDAHY+RG
Sbjct: 21 KTAVGGGVGDVYGEDRATEEQQVTPWTVSVASGYSLLRDPHHNKGLAFSEKERDAHYLRG 80
Query: 537 LLPPVVISQELQVKKMINAIRQYQVPLQKYIAMMDLQERNQKLFYKLLIEHVEELLPVVY 716
LLPP ISQE+QVKKM++ +RQYQVPLQ+Y+AMMDLQE N++LFYKLLI+HVEELLPVVY
Sbjct: 81 LLPPTCISQEIQVKKMLHNLRQYQVPLQRYMAMMDLQEMNERLFYKLLIDHVEELLPVVY 140
Query: 717 TPTVGEACQKYGSIFQRPQGLFISLKEKGKILEVLRNWPEKNIQVIVVTDGERILGLGDL 896
TPTVGEACQKYG IF+RPQGL+ISLKEKGKILEVL+NWPE+NIQVIVVTDGERILGLGDL
Sbjct: 141 TPTVGEACQKYGCIFRRPQGLYISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDL 200
Query: 897 GCHGMGIPVGKLSLYTAL 950
GC GMGIPVGKLSLYTAL
Sbjct: 201 GCQGMGIPVGKLSLYTAL 218
Database: GenBank nr
Posted date: Wed Dec 03 02:04:20 2008
Number of letters in database: 2,551,671,255
Number of sequences in database: 7,387,702
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 340,404,849,014
Number of Sequences: 7387702
Number of Extensions: 340404849014
Number of Successful Extensions: 174486074
Number of sequences better than 0.0: 0
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