BLASTX 7.6.2
Query= RU01503 /QuerySize=1539
(1538 letters)
Database: UniProt/Swiss-Prot;
462,764 sequences; 163,773,382 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O65355|GGH_ARATH Gamma-glutamyl hydrolase OS=Arabidopsis thal... 240 2e-062
sp|P93164|GGH_SOYBN Gamma-glutamyl hydrolase OS=Glycine max PE=2... 190 4e-047
sp|Q54LN4|GGHA_DICDI Gamma-glutamyl hydrolase A OS=Dictyostelium... 126 5e-028
sp|Q54HL4|GGHB_DICDI Gamma-glutamyl hydrolase B OS=Dictyostelium... 124 3e-027
sp|A7YWG4|GGH_BOVIN Gamma-glutamyl hydrolase OS=Bos taurus GN=GG... 109 8e-023
sp|Q92820|GGH_HUMAN Gamma-glutamyl hydrolase OS=Homo sapiens GN=... 107 2e-022
sp|Q9Z0L8|GGH_MOUSE Gamma-glutamyl hydrolase OS=Mus musculus GN=... 101 1e-020
sp|Q62867|GGH_RAT Gamma-glutamyl hydrolase OS=Rattus norvegicus ... 100 3e-020
>sp|O65355|GGH_ARATH Gamma-glutamyl hydrolase OS=Arabidopsis thaliana GN=GGH
PE=1 SV=2
Length = 347
Score = 240 bits (612), Expect = 2e-062
Identities = 117/180 (65%), Positives = 136/180 (75%), Gaps = 1/180 (0%)
Frame = +3
Query: 753 FSIICHLLGFRTSTMIISQNKKILESFSAADMASTLQFTE-TNIEGTVFQRFPPDLLRKL 929
F I LGF TMIISQN+ I E A + AS+LQF E NI+GT+FQRFPP+LL+KL
Sbjct: 149 FPIYAICLGFELLTMIISQNRDIFEKMDARNSASSLQFVENVNIQGTIFQRFPPELLKKL 208
Query: 930 STDCIVMQNHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWH 1109
TDC+VMQNH +GISP++ E + LS+FFKI+TT D + KVYVSTV + YPVT FQWH
Sbjct: 209 GTDCLVMQNHRFGISPQSFEGNIALSNFFKIVTTCVDDNGKVYVSTVQSTKYPVTGFQWH 268
Query: 1110 PEKNAFEWGLPMIPHSEDAIHVTQHIANFFISEARKSLNRPPIQDVLDNLIYNYSPTFCG 1289
PEKNAFEWG IPHSEDAI VTQH AN +SEARKSLNRP + VL NLIYNY PT+CG
Sbjct: 269 PEKNAFEWGSSKIPHSEDAIQVTQHAANHLVSEARKSLNRPESKKVLSNLIYNYKPTYCG 328
Score = 215 bits (545), Expect = 1e-054
Identities = 108/163 (66%), Positives = 127/163 (77%), Gaps = 4/163 (2%)
Frame = +1
Query: 310 MWNYLWIPFLISLSKELTSAKAQSTPTILLPSQLAGSDSSQR--CTALDPKLNYRPVIGI 483
MW+Y+W+P L++LS S TILLPSQ G D S+ C+A DP LNYRPVIGI
Sbjct: 1 MWSYVWLP-LVALSLFKDSIIMAKAATILLPSQ-TGFDISRSPVCSAPDPNLNYRPVIGI 58
Query: 484 LSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPLIYNEPSDVLFQKLNLVNGVLF 663
LSHPGDGASGRLSNAT S IAASYVK ES GARVIPLI+NEP ++LFQKL LVNGV+
Sbjct: 59 LSHPGDGASGRLSNATDASSIAASYVKLAESGGARVIPLIFNEPEEILFQKLELVNGVIL 118
Query: 664 TGGWAKSGRYYGVAEKIFKMILKKNDAGDHFPLYAICLGFELL 792
TGGWAK G Y+ + +KIF +L++NDAG+HFP+YAICLGFELL
Sbjct: 119 TGGWAKEGLYFEIVKKIFNKVLERNDAGEHFPIYAICLGFELL 161
>sp|P93164|GGH_SOYBN Gamma-glutamyl hydrolase OS=Glycine max PE=2 SV=1
Length = 342
Score = 190 bits (480), Expect = 4e-047
Identities = 98/186 (52%), Positives = 121/186 (65%), Gaps = 2/186 (1%)
Frame = +3
Query: 753 FSIICHLLGFRTSTMIISQNKKILESFSAADMASTL-QFTETNIEGTVFQRFPPDLLRKL 929
F +I LG I+S+ ILE F+A+ + S+L + E N +G++FQRFP DLL +L
Sbjct: 149 FPVIAFNLGGNLVIRIVSEQTDILEPFTASSLPSSLVLWNEANAKGSLFQRFPSDLLTQL 208
Query: 930 STDCIVMQNHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWH 1109
TDC+V+ NH Y ISP L+ + LS FF+IL TS D+D K +VST YPVT W
Sbjct: 209 KTDCLVLHNHRYAISPRKLQYNTKLSDFFEILATSGDRDGKTFVSTARGRKYPVTVNLWQ 268
Query: 1110 PEKNAFEWGLPM-IPHSEDAIHVTQHIANFFISEARKSLNRPPIQDVLDNLIYNYSPTFC 1286
PEKNAFEW + PH+EDAI VTQ ANFFISEARKS N P Q V D+LIYNY PTF
Sbjct: 269 PEKNAFEWATSLKAPHTEDAIRVTQSTANFFISEARKSTNTPDAQKVRDSLIYNYKPTFG 328
Query: 1287 GKGREG 1304
G +G
Sbjct: 329 GTAGKG 334
Score = 184 bits (467), Expect = 1e-045
Identities = 100/153 (65%), Positives = 112/153 (73%), Gaps = 2/153 (1%)
Frame = +1
Query: 334 FLISLSKELTSAKAQSTPTILLPSQLAGSDSSQRCTALDPKLNYRPVIGILSHPGDGASG 513
F ++L L SA + I LPSQL D S CTA DP LNY+PVIGIL+HPGDGASG
Sbjct: 11 FFVTLFTCLLSATSHD-DHIFLPSQL-HDDDSVSCTATDPSLNYKPVIGILTHPGDGASG 68
Query: 514 RLSNATGTSYIAASYVKFVESAGARVIPLIYNEPSDVLFQKLNLVNGVLFTGGWAKSGRY 693
RLSNATG SYIAASYVKFVES GARVIPLIYNE + L +KL+LVNGVLFTGGWA SG Y
Sbjct: 69 RLSNATGVSYIAASYVKFVESGGARVIPLIYNESPENLNKKLDLVNGVLFTGGWAVSGPY 128
Query: 694 YGVAEKIFKMILKKNDAGDHFPLYAICLGFELL 792
IFK L++NDAGDHFP+ A LG L+
Sbjct: 129 LDTLGNIFKKALERNDAGDHFPVIAFNLGGNLV 161
>sp|Q54LN4|GGHA_DICDI Gamma-glutamyl hydrolase A OS=Dictyostelium discoideum
GN=gghA PE=3 SV=1
Length = 317
Score = 126 bits (315), Expect = 5e-028
Identities = 58/174 (33%), Positives = 105/174 (60%), Gaps = 6/174 (3%)
Frame = +3
Query: 774 LGFRTSTMIISQNKKILESFSAADMASTLQFTETNIEGTVFQRFPPDLLRKLSTDCIVMQ 953
+GF+ ++ + N +L S+++ + L FT +F +++ L+++ I M
Sbjct: 131 MGFQELALLSADNFNLLSSYNSENYTVPLNFTSLAAGSRLFSLASSSIMQSLASEPITMN 190
Query: 954 NHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWHPEKNAFE- 1130
NH +G+SP+T +++ +++FF +L+T+ D+D ++ST+ A +YP+ QWHPEK FE
Sbjct: 191 NHQFGLSPQTYQQTSSINTFFDVLSTNVDRDGNTFISTIEAKNYPIYGTQWHPEKPIFEW 250
Query: 1131 WGLPMIPHSEDAIHVTQHIANFFISEARKSLNR---PPIQDVLDNLIYNYSPTF 1283
W ++ HS D+I Q+ +NFF++E RKSL+ P ++ LIYNY+P +
Sbjct: 251 WDQEVMNHSFDSIMANQYTSNFFVNECRKSLHSFSDPSVE--ASTLIYNYTPQY 302
Score = 95 bits (234), Expect = 1e-018
Identities = 50/121 (41%), Positives = 73/121 (60%), Gaps = 7/121 (5%)
Frame = +1
Query: 454 KLNYRPVIGILSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPLIYNEPSDVLFQ 633
K+N RP+IGIL+ P DG G+ YIAASYVK++ESAGARV+P++Y+ L +
Sbjct: 25 KINNRPIIGILTQPTDGD----MTTFGSQYIAASYVKYIESAGARVVPILYDIDIKSLTE 80
Query: 634 KLNLVNGVLFTGGWAKSGR---YYGVAEKIFKMILKKNDAGDHFPLYAICLGFELLQ*SS 804
+ +NGV F GG Y + I+ +++ N+ GD+FPL+ C+GF+ L S
Sbjct: 81 LMGSINGVFFPGGGVDFNNQTVYTDTIQSIWSQVVEFNNNGDYFPLWGTCMGFQELALLS 140
Query: 805 A 807
A
Sbjct: 141 A 141
>sp|Q54HL4|GGHB_DICDI Gamma-glutamyl hydrolase B OS=Dictyostelium discoideum
GN=gghB PE=3 SV=1
Length = 347
Score = 124 bits (309), Expect = 3e-027
Identities = 60/175 (34%), Positives = 102/175 (58%), Gaps = 2/175 (1%)
Frame = +3
Query: 774 LGFRTSTMIISQNKKILESFSAADMASTLQFTETNIEGTVFQRFPPDLLRKLSTDCIVMQ 953
LG + +++ +L F+A + + L F+ +E + + P +++ L+ D I M
Sbjct: 129 LGLEEIVSLQAESFDVLTDFNAENYSIPLNFSNIALESKIMKNCPTNIINSLANDPITMN 188
Query: 954 NHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWHPEKNAFEW 1133
NHH+GISP T + + L+ FF +L T+ DK ++S + + YP+ A WHPEK+ + W
Sbjct: 189 NHHFGISPNTFDNNSLLNQFFNVLATNNDKSGNEFISLIESKDYPIYAIIWHPEKSPYSW 248
Query: 1134 -GLPMIPHSEDAIHVTQHIANFFISEARKSLNRPPIQDVL-DNLIYNYSPTFCGK 1292
HS +AI Q+++NFF++E RKS ++ ++VL +LIYNY+PT+ K
Sbjct: 249 YSKDATDHSFNAILACQYMSNFFVNETRKSNHKFNDEEVLFKSLIYNYNPTYTFK 303
Score = 82 bits (200), Expect = 1e-014
Identities = 45/113 (39%), Positives = 65/113 (57%), Gaps = 6/113 (5%)
Frame = +1
Query: 457 LNYRPVIGILSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPLIYNEPSDVLFQK 636
+N PVIGIL+ P + ++ G +Y+ ASYVK+VESAGARV+P+ YN+ + L
Sbjct: 23 INNTPVIGILTQPFPSS---INIKYGDNYLMASYVKYVESAGARVVPIFYNQDDESLTTI 79
Query: 637 LNLVNGVLFTGG---WAKSGRYYGVAEKIFKMILKKNDAGDHFPLYAICLGFE 786
+NG+L GG + +Y I+ +L N GD+FPL+ CLG E
Sbjct: 80 FKQINGILLPGGDVDFKTEIQYVKTLTLIWDYVLDVNINGDYFPLWGTCLGLE 132
>sp|A7YWG4|GGH_BOVIN Gamma-glutamyl hydrolase OS=Bos taurus GN=GGH PE=2 SV=1
Length = 318
Score = 109 bits (270), Expect = 8e-023
Identities = 62/175 (35%), Positives = 96/175 (54%), Gaps = 6/175 (3%)
Frame = +3
Query: 774 LGFRTSTMIISQNKKILESFSAADMASTLQFTETNIEGTVFQRFPPDLLRKLSTDCIVMQ 953
LGF ++S + +L + L F+ ++ +FQ FP DLL L+ + +
Sbjct: 135 LGFEELIYLVS-GESLLTLTDTVGIKLPLNFSRGTLQSRMFQNFPADLLLSLAVEPLTAH 193
Query: 954 NHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWHPEKNAFEW 1133
H + +S +++ L +FF ILTT+TD + ++ST+ Y YP+ QWHPEK +EW
Sbjct: 194 FHKWSLSVMNFTKNEKLKAFFSILTTNTDGNID-FISTMEGYRYPIYGVQWHPEKAPYEW 252
Query: 1134 G-LPMIPHSEDAIHVTQHIANFFISEARKSLNRPPIQDVLDN--LIYNYSPTFCG 1289
G L I H+ +A+ ++A FF++EARKS N DV + LIY Y PT+ G
Sbjct: 253 GQLRGISHAPNAVKAAFYLAEFFVAEARKS-NHHFESDVEETKALIYQYRPTYTG 306
Score = 81 bits (198), Expect = 2e-014
Identities = 51/114 (44%), Positives = 68/114 (59%), Gaps = 11/114 (9%)
Frame = +1
Query: 466 RPVIGILSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPL---IYNEPSDVLFQK 636
+P+IGIL + R A G YIAASYVKF+ESAGARV+P+ + NE + LF+
Sbjct: 33 KPIIGILMQKCHNKNMR---ALGKYYIAASYVKFLESAGARVVPVRLDLKNEEYEKLFKS 89
Query: 637 LNLVNGVLFTGGWAKSGR--YYGVAEKIFKMILKKNDAGDHFPLYAICLGFELL 792
+NGVLF GG R Y VA+ + + +K GD+FP++ CLGFE L
Sbjct: 90 ---INGVLFPGGSVNLMRSGYARVAKMFYNLSIKSFGEGDYFPVWGTCLGFEEL 140
>sp|Q92820|GGH_HUMAN Gamma-glutamyl hydrolase OS=Homo sapiens GN=GGH PE=1 SV=2
Length = 318
Score = 107 bits (267), Expect = 2e-022
Identities = 59/174 (33%), Positives = 97/174 (55%), Gaps = 4/174 (2%)
Frame = +3
Query: 774 LGFRTSTMIISQNKKILESFSAADMASTLQFTETNIEGTVFQRFPPDLLRKLSTDCIVMQ 953
LGF +++IS + +L + D+A L FT + +FQ FP +LL L+ + +
Sbjct: 135 LGFEELSLLIS-GECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTAN 193
Query: 954 NHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWHPEKNAFEW 1133
H + +S + ++ L FF +LTT+TD + ++ST+ Y YPV QWHPEK +EW
Sbjct: 194 FHKWSLSVKNFTMNEKLKKFFNVLTTNTDGKIE-FISTMEGYKYPVYGVQWHPEKAPYEW 252
Query: 1134 -GLPMIPHSEDAIHVTQHIANFFISEARKSLNRPPIQDVLDN-LIYNYSPTFCG 1289
L I H+ +A+ ++A FF++EARK+ + + + LIY +SP + G
Sbjct: 253 KNLDGISHAPNAVKTAFYLAEFFVNEARKNNHHFKSESEEEKALIYQFSPIYTG 306
Score = 76 bits (185), Expect = 6e-013
Identities = 45/114 (39%), Positives = 68/114 (59%), Gaps = 11/114 (9%)
Frame = +1
Query: 466 RPVIGILSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPL---IYNEPSDVLFQK 636
+P+IGIL ++ G YIAASYVK++ESAGARV+P+ + + ++LF+
Sbjct: 33 KPIIGILMQK---CRNKVMKNYGRYYIAASYVKYLESAGARVVPVRLDLTEKDYEILFKS 89
Query: 637 LNLVNGVLFTGGWAKSGR--YYGVAEKIFKMILKKNDAGDHFPLYAICLGFELL 792
+NG+LF GG R Y VA+ + + ++ D GD+FP++ CLGFE L
Sbjct: 90 ---INGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEEL 140
>sp|Q9Z0L8|GGH_MOUSE Gamma-glutamyl hydrolase OS=Mus musculus GN=Ggh PE=1 SV=1
Length = 317
Score = 101 bits (251), Expect = 1e-020
Identities = 60/176 (34%), Positives = 97/176 (55%), Gaps = 8/176 (4%)
Frame = +3
Query: 774 LGFRTSTMIISQNKKILESFSAADMASTLQFTETNIEGTVFQRFPPDLLRKLSTDCIVMQ 953
LGF ++++S + +L S L FTE + +F+ FP +LL L+ + +
Sbjct: 134 LGFEELSVLVS-GENLLTSTDTKSKKLPLNFTEGARKNKMFKHFPTELLDSLALENLTAN 192
Query: 954 NHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWHPEKNAFEW 1133
H + +S + E++ L FF ILTT+TD + ++S++ + YPV A QWHPEK FEW
Sbjct: 193 FHKWSLSVKNFTENEKLKKFFNILTTNTDGKTE-FISSMEGFKYPVYAVQWHPEKAPFEW 251
Query: 1134 -GLPMIPHSEDAIHVTQHIANFFISEARKSLNRPPIQDVLD---NLIYNYSPTFCG 1289
L I H+ +A+ + ++A F +SEARK N ++V+ +LIY P + G
Sbjct: 252 KNLGGISHAPNAVKTSFYLAEFLVSEARK--NSHHFENVVKETASLIYKVYPIYTG 305
Score = 80 bits (196), Expect = 3e-014
Identities = 50/112 (44%), Positives = 66/112 (58%), Gaps = 8/112 (7%)
Frame = +1
Query: 466 RPVIGILSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPLIYNEPSDVLFQKL-N 642
RP+IG++ G +L G YIAASYVK++ESAGARV+P I + SD +++L
Sbjct: 33 RPIIGVVMQECFGKMAKL----GNYYIAASYVKYIESAGARVVP-IRPDLSDAEYEELFR 87
Query: 643 LVNGVLFTGGWAK--SGRYYGVAEKIFKMILKKNDAGDHFPLYAICLGFELL 792
+NGVL GG A Y VA+ F L+ D G HFP++ CLGFE L
Sbjct: 88 SINGVLLPGGGANLTDSGYSRVAKIFFSKALESFDNGGHFPVWGTCLGFEEL 139
>sp|Q62867|GGH_RAT Gamma-glutamyl hydrolase OS=Rattus norvegicus GN=Ggh PE=1
SV=1
Length = 317
Score = 100 bits (248), Expect = 3e-020
Identities = 55/174 (31%), Positives = 98/174 (56%), Gaps = 4/174 (2%)
Frame = +3
Query: 774 LGFRTSTMIISQNKKILESFSAADMASTLQFTETNIEGTVFQRFPPDLLRKLSTDCIVMQ 953
LG ++++S N +L + + + L FT + + +F+ P +LL L+++ +
Sbjct: 134 LGLEELSVLVS-NDNLLTLTNTSSVKLPLNFTRDSKQSRMFRNLPEELLNSLASENLTAN 192
Query: 954 NHHYGISPETLEESQDLSSFFKILTTSTDKDDKVYVSTVHAYHYPVTAFQWHPEKNAFEW 1133
H + +S + E++ L FF ILT +TD + ++S++ Y YP+ A QWHPEK FEW
Sbjct: 193 FHKWSLSVKNFTENEKLKKFFNILTVNTDGKTE-FISSMEGYKYPIYAVQWHPEKAPFEW 251
Query: 1134 -GLPMIPHSEDAIHVTQHIANFFISEARKSLNRPPIQ-DVLDNLIYNYSPTFCG 1289
L I H+ +A+ + ++A FFISEA K+ + + + ++LIY + P + G
Sbjct: 252 KKLRGISHAPNAVKTSFYLAKFFISEALKNDHHFENELEETESLIYQFCPVYTG 305
Score = 76 bits (185), Expect = 6e-013
Identities = 50/112 (44%), Positives = 65/112 (58%), Gaps = 8/112 (7%)
Frame = +1
Query: 466 RPVIGILSHPGDGASGRLSNATGTSYIAASYVKFVESAGARVIPLIYNEPSDVLFQKL-N 642
RP+IGI+ G +L G YIAASYVKF+ESAGARV+P I + +D ++ L
Sbjct: 33 RPIIGIIMQECYGNMTKL----GRFYIAASYVKFIESAGARVVP-IRLDLNDAQYETLFR 87
Query: 643 LVNGVLFTGGWAK--SGRYYGVAEKIFKMILKKNDAGDHFPLYAICLGFELL 792
+NGVL GG A Y VA+ F L+ D GD+FP++ CLG E L
Sbjct: 88 SINGVLLPGGGANLTHSGYSRVAKIFFTKALESFDNGDYFPVWGTCLGLEEL 139
Database: UniProt/Swiss-Prot
Posted date: Thu Apr 23 14:22:33 2009
Number of letters in database: 163,773,382
Number of sequences in database: 462,764
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,055,803,260
Number of Sequences: 462764
Number of Extensions: 3055803260
Number of Successful Extensions: 28798457
Number of sequences better than 0.0: 0
|