BLASTX 7.6.2
Query= RU05313 /QuerySize=548
(547 letters)
Database: UniProt/Swiss-Prot;
462,764 sequences; 163,773,382 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P46313|FAD6E_ARATH Omega-6 fatty acid desaturase, endoplasmic... 213 8e-055
sp|Q39287|FAD6E_BRAJU Omega-6 fatty acid desaturase, endoplasmic... 206 6e-053
sp|P48631|FD6E2_SOYBN Omega-6 fatty acid desaturase, endoplasmic... 200 7e-051
sp|P48630|FD6E1_SOYBN Omega-6 fatty acid desaturase, endoplasmic... 184 2e-046
sp|O81931|FAD12_CREAL Delta(12) fatty acid dehydrogenase OS=Crep... 159 1e-038
sp|Q9Y8H5|FAD12_MORAP Delta(12) fatty acid desaturase OS=Mortier... 111 3e-024
sp|P59668|FAD12_MORIS Delta(12) fatty acid desaturase OS=Mortier... 111 3e-024
sp|P48623|FAD3E_ARATH Omega-3 fatty acid desaturase, endoplasmic... 97 7e-020
sp|P48618|FAD3C_BRANA Omega-3 fatty acid desaturase, chloroplast... 89 1e-017
sp|P48621|FAD3C_SOYBN Omega-3 fatty acid desaturase, chloroplast... 88 3e-017
sp|P46310|FAD3C_ARATH Omega-3 fatty acid desaturase, chloroplast... 87 5e-017
sp|P48622|FAD3D_ARATH Temperature-sensitive omega-3 fatty acid d... 50 5e-006
>sp|P46313|FAD6E_ARATH Omega-6 fatty acid desaturase, endoplasmic reticulum
OS=Arabidopsis thaliana GN=FAD2 PE=2 SV=1
Length = 383
Score = 213 bits (540), Expect = 8e-055
Identities = 93/133 (69%), Positives = 110/133 (82%)
Frame = +1
Query: 133 MGAGGRMSAPPTRKNAETDNIKRVPHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDL 312
MGAGGRM P + K +ETD KRVP KPPF++G +KKAIPPHCF+RS+ SFSY++ D+
Sbjct: 1 MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60
Query: 313 AIASIFYYIASNYINHLSTPLSFLAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDD 492
IAS FYY+A+NY + L PLS+LAWP+YWA QGCVLTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120
Query: 493 TVGLVLHSCLLFP 531
TVGL+ HS LL P
Sbjct: 121 TVGLIFHSFLLVP 133
>sp|Q39287|FAD6E_BRAJU Omega-6 fatty acid desaturase, endoplasmic reticulum
OS=Brassica juncea PE=2 SV=1
Length = 384
Score = 206 bits (524), Expect = 6e-053
Identities = 90/133 (67%), Positives = 109/133 (81%)
Frame = +1
Query: 133 MGAGGRMSAPPTRKNAETDNIKRVPHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDL 312
MGAGGRM P+ K +ETD +KRVP PPFT+G++KKAIPPHCF+RS+ SFSY+++D+
Sbjct: 1 MGAGGRMQVSPSPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60
Query: 313 AIASIFYYIASNYINHLSTPLSFLAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDD 492
+AS FYY+A+ Y L PLS++AWP+YWA QG VLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61 IVASCFYYVATTYFPLLPHPLSYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 493 TVGLVLHSCLLFP 531
TVGL+ HS LL P
Sbjct: 121 TVGLIFHSFLLVP 133
>sp|P48631|FD6E2_SOYBN Omega-6 fatty acid desaturase, endoplasmic reticulum
isozyme 2 OS=Glycine max GN=FAD2-2 PE=2 SV=1
Length = 383
Score = 200 bits (506), Expect = 7e-051
Identities = 91/133 (68%), Positives = 104/133 (78%)
Frame = +1
Query: 133 MGAGGRMSAPPTRKNAETDNIKRVPHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDL 312
MGAGGR PP + +E D +KRVP KP F+L Q+KKAIPPHCFQRSV+ SFSYVVYDL
Sbjct: 1 MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 313 AIASIFYYIASNYINHLSTPLSFLAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDD 492
IA YY+A++Y + L PLSF IYWA+QGC+LTGVWVIAHECGHHAFSDYQ LDD
Sbjct: 61 TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120
Query: 493 TVGLVLHSCLLFP 531
VGL+LHS LL P
Sbjct: 121 IVGLILHSALLVP 133
>sp|P48630|FD6E1_SOYBN Omega-6 fatty acid desaturase, endoplasmic reticulum
isozyme 1 OS=Glycine max GN=FAD2-1 PE=2 SV=1
Length = 387
Score = 184 bits (467), Expect = 2e-046
Identities = 87/134 (64%), Positives = 102/134 (76%), Gaps = 4/134 (2%)
Frame = +1
Query: 130 TMGAGGRMSAPPTRKNAETDNIKRVPHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYD 309
TMG GR++ + + RVP+ KPPFT+GQ+KKAIPPHCFQRS++ SFSYVVYD
Sbjct: 8 TMGGRGRVAKVEVQGKKP---LSRVPNTKPPFTVGQLKKAIPPHCFQRSLLTSFSYVVYD 64
Query: 310 LAIASIFYYIASNYINHLSTPLSFLAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLD 489
L+ A IF YIA+ Y + L P S +AWPIYW LQGC+LTGVWVIAHECGHHAFS YQW+D
Sbjct: 65 LSFAFIF-YIATTYFHLLPQPFSLIAWPIYWVLQGCLLTGVWVIAHECGHHAFSKYQWVD 123
Query: 490 DTVGLVLHSCLLFP 531
D VGL LHS LL P
Sbjct: 124 DVVGLTLHSTLLVP 137
>sp|O81931|FAD12_CREAL Delta(12) fatty acid dehydrogenase OS=Crepis alpina PE=2
SV=2
Length = 375
Score = 159 bits (400), Expect = 1e-038
Identities = 67/105 (63%), Positives = 83/105 (79%)
Frame = +1
Query: 217 PPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIASNYINHLSTPLSFLAWPI 396
PPFT+ +K+AIPPHCF+RSVI S Y+V+D IA IFY++A YI L PL++LAWP+
Sbjct: 22 PPFTVSDLKQAIPPHCFKRSVIRSSYYIVHDAIIAYIFYFLADKYIPILPAPLAYLAWPL 81
Query: 397 YWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVLHSCLLFP 531
YW Q +LTG+WVI HECGHHAFSDYQW+DDTVG +LHS L+ P
Sbjct: 82 YWFCQASILTGLWVIGHECGHHAFSDYQWVDDTVGFILHSFLMTP 126
>sp|Q9Y8H5|FAD12_MORAP Delta(12) fatty acid desaturase OS=Mortierella alpina
PE=2 SV=2
Length = 400
Score = 111 bits (277), Expect = 3e-024
Identities = 50/106 (47%), Positives = 72/106 (67%), Gaps = 2/106 (1%)
Frame = +1
Query: 217 PPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIASNYINHLSTPL-SFLAWP 393
P FT+ ++++ IP HCF+RS + +V DL AS+ ++A+ I+ PL +LAWP
Sbjct: 36 PEFTIKEIRECIPAHCFERSGLRGLCHVAIDLTWASLL-FLAATQIDKFENPLIRYLAWP 94
Query: 394 IYWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVLHSCLLFP 531
YW +QG V TG+WV+AHECGH +FS + L++TVG +LHS LL P
Sbjct: 95 AYWIMQGIVCTGIWVLAHECGHQSFSTSKTLNNTVGWILHSMLLVP 140
>sp|P59668|FAD12_MORIS Delta(12) fatty acid desaturase OS=Mortierella isabellina
PE=2 SV=1
Length = 400
Score = 111 bits (277), Expect = 3e-024
Identities = 50/106 (47%), Positives = 72/106 (67%), Gaps = 2/106 (1%)
Frame = +1
Query: 217 PPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIASNYINHLSTPL-SFLAWP 393
P FT+ ++++ IP HCF+RS + +V DL AS+ ++A+ I+ PL +LAWP
Sbjct: 36 PEFTIKEIRECIPAHCFERSGLRGLCHVAIDLTWASLL-FLAATQIDKFENPLIRYLAWP 94
Query: 394 IYWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVLHSCLLFP 531
YW +QG V TG+WV+AHECGH +FS + L++TVG +LHS LL P
Sbjct: 95 AYWIMQGIVCTGIWVLAHECGHQSFSTSKTLNNTVGWILHSMLLVP 140
>sp|P48623|FAD3E_ARATH Omega-3 fatty acid desaturase, endoplasmic reticulum
OS=Arabidopsis thaliana GN=FAD3 PE=2 SV=1
Length = 386
Score = 97 bits (239), Expect = 7e-020
Identities = 46/109 (42%), Positives = 68/109 (62%), Gaps = 7/109 (6%)
Frame = +1
Query: 205 PHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIASNYINHLSTPLSFL 384
P +PPF +G ++ AIP HC+ +S + S SYVV D+ IA IA+ Y++ S+
Sbjct: 28 PSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDI-IAVAALAIAAVYVD------SWF 80
Query: 385 AWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVLHSCLLFP 531
WP+YWA QG + ++V+ H+CGH +FSD L+ VG +LHS +L P
Sbjct: 81 LWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVP 129
>sp|P48618|FAD3C_BRANA Omega-3 fatty acid desaturase, chloroplastic (Fragment)
OS=Brassica napus GN=FAD7 PE=2 SV=1
Length = 404
Score = 89 bits (219), Expect = 1e-017
Identities = 48/127 (37%), Positives = 73/127 (57%), Gaps = 11/127 (8%)
Frame = +1
Query: 154 SAPPTRKNAETDNIKRVPHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFY 333
S+PP + +T P PPF L ++ AIP HC+ ++ S SYVV +LAI +F
Sbjct: 33 SSPPIEEEPKTQRFD--PGAPPPFNLADIRAAIPKHCWVKNPWKSMSYVVRELAI--VFA 88
Query: 334 YIA-SNYINHLSTPLSFLAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVL 510
A + Y+N ++L WP+YW QG + ++V+ H+CGH +FS+ L+ VG +L
Sbjct: 89 LAAGAAYLN------NWLVWPLYWIAQGTMFWALFVLGHDCGHGSFSNDPRLNSVVGHLL 142
Query: 511 HSCLLFP 531
HS +L P
Sbjct: 143 HSSILVP 149
>sp|P48621|FAD3C_SOYBN Omega-3 fatty acid desaturase, chloroplastic OS=Glycine
max GN=FAD7 PE=2 SV=1
Length = 453
Score = 88 bits (216), Expect = 3e-017
Identities = 44/109 (40%), Positives = 64/109 (58%), Gaps = 7/109 (6%)
Frame = +1
Query: 205 PHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIASNYINHLSTPLSFL 384
P PPF L ++ AIP HC+ + S SYVV D+ IA A+ Y+N ++L
Sbjct: 98 PGAPPPFNLADIRAAIPKHCWVKDPWRSMSYVVRDV-IAVFGLAAAAAYLN------NWL 150
Query: 385 AWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVLHSCLLFP 531
WP+YWA QG + ++V+ H+CGH +FS+ L+ VG +LHS +L P
Sbjct: 151 VWPLYWAAQGTMFWALFVLGHDCGHGSFSNNSKLNSVVGHLLHSSILVP 199
>sp|P46310|FAD3C_ARATH Omega-3 fatty acid desaturase, chloroplastic
OS=Arabidopsis thaliana GN=FAD7 PE=1 SV=1
Length = 446
Score = 87 bits (214), Expect = 5e-017
Identities = 47/119 (39%), Positives = 71/119 (59%), Gaps = 10/119 (8%)
Frame = +1
Query: 181 ETDNIKRV-PHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIA-SNYI 354
E DN +R P PPF L ++ AIP HC+ ++ S SYVV D+AI +F A + Y+
Sbjct: 81 EEDNKQRFDPGAPPPFNLADIRAAIPKHCWVKNPWKSLSYVVRDVAI--VFALAAGAAYL 138
Query: 355 NHLSTPLSFLAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLVLHSCLLFP 531
N +++ WP+YW QG + ++V+ H+CGH +FS+ L+ VG +LHS +L P
Sbjct: 139 N------NWIVWPLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVP 191
>sp|P48622|FAD3D_ARATH Temperature-sensitive omega-3 fatty acid desaturase,
chloroplastic OS=Arabidopsis thaliana GN=FAD8 PE=2 SV=1
Length = 435
Score = 50 bits (119), Expect = 5e-006
Identities = 29/78 (37%), Positives = 43/78 (55%), Gaps = 1/78 (1%)
Frame = +1
Query: 178 AETDNIKRVPHLKPPFTLGQVKKAIPPHCFQRSVIHSFSYVVYDLAIASIFYYIASNYIN 357
+E D + P PPF L ++ AIP HC+ ++ S SYVV D+AI +A+ + N
Sbjct: 74 SEEDTERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVAAYFNN 133
Query: 358 HLSTPLSFLA-WPIYWAL 408
L PL + A ++WAL
Sbjct: 134 WLLWPLYWFAQGTMFWAL 151
Database: UniProt/Swiss-Prot
Posted date: Thu Apr 23 14:22:33 2009
Number of letters in database: 163,773,382
Number of sequences in database: 462,764
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,390,147,223
Number of Sequences: 462764
Number of Extensions: 8390147223
Number of Successful Extensions: 74969301
Number of sequences better than 0.0: 0
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