BLASTX 7.6.2
Query= RU06732 /QuerySize=840
(839 letters)
Database: UniProt/Swiss-Prot;
462,764 sequences; 163,773,382 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q93WU3|CVMT1_OCIBA Chavicol O-methyltransferase OS=Ocimum bas... 134 8e-031
sp|Q93WU2|EOMT1_OCIBA Eugenol O-methyltransferase OS=Ocimum basi... 133 2e-030
sp|O22308|7OMT6_MEDSA Isoflavone-7-O-methyltransferase 6 OS=Medi... 107 8e-023
sp|O24529|7OMT8_MEDSA Isoflavone-7-O-methyltransferase 8 OS=Medi... 107 8e-023
sp|O22309|7OMT9_MEDSA Isoflavone-7-O-methyltransferase 9 OS=Medi... 106 2e-022
sp|Q8H9A8|COOMT_COPJA Columbamine O-methyltransferase OS=Coptis ... 90 2e-017
sp|P47917|ZRP4_MAIZE O-methyltransferase ZRP4 OS=Zea mays GN=ZRP... 87 9e-017
sp|Q9LEL6|6OMT_COPJA (RS)-norcoclaurine 6-O-methyltransferase OS... 76 2e-013
sp|Q9LEL5|4OMT_COPJA 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-met... 73 2e-012
>sp|Q93WU3|CVMT1_OCIBA Chavicol O-methyltransferase OS=Ocimum basilicum
GN=CVOMT1 PE=1 SV=1
Length = 356
Score = 134 bits (336), Expect = 8e-031
Identities = 69/133 (51%), Positives = 93/133 (69%), Gaps = 2/133 (1%)
Frame = +3
Query: 279 SNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHPTKSH 458
+ +LL AQAH+WNH+++F NSMSLK AIQLGIPDI++KH +PMTLS+L A+PI+ KS
Sbjct: 12 TEQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHDHPMTLSQLLKAIPINKEKSQ 71
Query: 459 SVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPVFDQS 638
S RLMR LV+S FF ++ S E Y LT AS+LLLK PL++ P + +LDP F
Sbjct: 72 SFQRLMRALVNSNFFIEEN-SNNQEVCYWLTPASRLLLKGAPLTVAPLVQVVLDPTFTNP 130
Query: 639 MGITWSTWFQNDD 677
S WF++++
Sbjct: 131 WHY-MSEWFKHEN 142
>sp|Q93WU2|EOMT1_OCIBA Eugenol O-methyltransferase OS=Ocimum basilicum GN=EOMT1
PE=1 SV=1
Length = 357
Score = 133 bits (333), Expect = 2e-030
Identities = 67/132 (50%), Positives = 90/132 (68%), Gaps = 1/132 (0%)
Frame = +3
Query: 279 SNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHPTKSH 458
+ +LL AQ H+WNH+++F NSMSLK AIQLGIPDI++KHG PMTLS+L ++PI+ K+
Sbjct: 12 TEQLLQAQVHVWNHMYAFANSMSLKCAIQLGIPDILHKHGRPMTLSQLLQSIPINKEKTQ 71
Query: 459 SVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPVFDQS 638
RLMR LV+S FF ++ S E Y LT AS LLLK+ PL++TP + +LDP F
Sbjct: 72 CFQRLMRALVNSNFFIEENNSNNQEVCYWLTPASCLLLKEAPLTVTPLVQVVLDPTFTNP 131
Query: 639 MGITWSTWFQND 674
S WF ++
Sbjct: 132 WH-HMSEWFTHE 142
>sp|O22308|7OMT6_MEDSA Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa
PE=2 SV=1
Length = 352
Score = 107 bits (267), Expect = 8e-023
Identities = 66/169 (39%), Positives = 99/169 (58%), Gaps = 6/169 (3%)
Frame = +3
Query: 267 NGEHSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHP 446
NG +E+ AQA ++ HI++FI+SMSLK A+++ IP+II HG P++LS L S L +
Sbjct: 6 NGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPS 65
Query: 447 TKSHSVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPV 626
+K +V RLMR L H+GFF ++ +EE Y LT AS+LL++ L L P + +LDP
Sbjct: 66 SKIGNVRRLMRYLAHNGFF---EIITKEEESYALTVASELLVRGSDLCLAPMVECVLDPT 122
Query: 627 FDQSMGITWSTWFQNDDPTPI*HSTWG*HFGDYGNHSAKFLPIWFNDAL 773
S W +D T + T G F D+ + + ++ FNDA+
Sbjct: 123 LSGSYH-ELKKWIYEEDLT-LFGVTLGSGFWDFLDKNPEY-NTSFNDAM 168
>sp|O24529|7OMT8_MEDSA Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa
PE=1 SV=1
Length = 352
Score = 107 bits (267), Expect = 8e-023
Identities = 66/169 (39%), Positives = 99/169 (58%), Gaps = 6/169 (3%)
Frame = +3
Query: 267 NGEHSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHP 446
NG +E+ AQA ++ HI++FI+SMSLK A+++ IP+II HG P++LS L S L +
Sbjct: 6 NGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPS 65
Query: 447 TKSHSVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPV 626
+K +V RLMR L H+GFF ++ +EE Y LT AS+LL++ L L P + +LDP
Sbjct: 66 SKIGNVRRLMRYLAHNGFF---EIITKEEESYALTVASELLVRGSDLCLAPMVECVLDPT 122
Query: 627 FDQSMGITWSTWFQNDDPTPI*HSTWG*HFGDYGNHSAKFLPIWFNDAL 773
S W +D T + T G F D+ + + ++ FNDA+
Sbjct: 123 LSGSYH-ELKKWIYEEDLT-LFGVTLGSGFWDFLDKNPEY-NTSFNDAM 168
>sp|O22309|7OMT9_MEDSA Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa
PE=2 SV=1
Length = 352
Score = 106 bits (264), Expect = 2e-022
Identities = 66/169 (39%), Positives = 99/169 (58%), Gaps = 6/169 (3%)
Frame = +3
Query: 267 NGEHSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHP 446
NG +E+ AQA ++ HI++FI+SMSLK A+ + IP+II+ HG P++LS L S L +
Sbjct: 6 NGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNLVSILQVPS 65
Query: 447 TKSHSVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPV 626
+K +V RLMR L H+GFF ++ +EE Y LT AS+LL++ L L P + +LDP
Sbjct: 66 SKIGNVRRLMRYLAHNGFF---EIITKEEESYALTVASELLVRGSDLCLAPMVECVLDPT 122
Query: 627 FDQSMGITWSTWFQNDDPTPI*HSTWG*HFGDYGNHSAKFLPIWFNDAL 773
S W +D T + T G F D+ + + ++ FNDA+
Sbjct: 123 LSGSYH-ELKKWIYEEDLT-LFGVTLGSGFWDFLDKNPEY-NTSFNDAM 168
>sp|Q8H9A8|COOMT_COPJA Columbamine O-methyltransferase OS=Coptis japonica PE=1
SV=1
Length = 351
Score = 90 bits (221), Expect = 2e-017
Identities = 47/119 (39%), Positives = 68/119 (57%), Gaps = 1/119 (0%)
Frame = +3
Query: 267 NGEHSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHP 446
N +++ + AQA +W H+F F ++ L+S + LGIPDII+ +G P+TLS+L + LP+
Sbjct: 5 NTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKS 63
Query: 447 TKSHSVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDP 623
T + MR LVH F T E+ Y LT AS+LL+ H SL PY+ P
Sbjct: 64 TSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHP 122
>sp|P47917|ZRP4_MAIZE O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 SV=1
Length = 364
Score = 87 bits (215), Expect = 9e-017
Identities = 55/146 (37%), Positives = 79/146 (54%), Gaps = 7/146 (4%)
Frame = +3
Query: 267 NGEHSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHP 446
N LL AQ +W+ F+F+ SM+LKSAI L I D I+ HG +LS++ S + +HP
Sbjct: 6 NNSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILSKVHLHP 65
Query: 447 TKSHSVYRLMRILVHSGFFAKKKL---SKTDEEG-YTLTDASQLLL--KDHPLSLTPYLT 608
++ S+ RLMR+L + F + L S D E YTLT S+LL+ + L+ TP
Sbjct: 66 SRVSSLRRLMRVLTTTNVFGTQPLGGGSDDDSEPVYTLTPVSRLLIGSQSSQLAQTPLAA 125
Query: 609 AMLDPVFDQSMGITWSTWFQNDDPTP 686
+LDP WFQ++ P P
Sbjct: 126 MVLDPTIVSPFS-ELGAWFQHELPDP 150
>sp|Q9LEL6|6OMT_COPJA (RS)-norcoclaurine 6-O-methyltransferase OS=Coptis
japonica PE=1 SV=1
Length = 347
Score = 76 bits (186), Expect = 2e-013
Identities = 44/119 (36%), Positives = 64/119 (53%), Gaps = 2/119 (1%)
Frame = +3
Query: 273 EHSNELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHPTK 452
E + L +QA +WN I+ F S+ LK A+QL + +II+ G MTLSEL+S LP P
Sbjct: 2 EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61
Query: 453 SHSVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPVF 629
++YR+MR LVH F K + E Y L ++ L+K + + A+ D F
Sbjct: 62 EDALYRVMRYLVHMKLFTKASID--GELRYGLAPPAKYLVKGWDKCMVGSILAITDKDF 118
>sp|Q9LEL5|4OMT_COPJA 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
OS=Coptis japonica PE=1 SV=1
Length = 350
Score = 73 bits (177), Expect = 2e-012
Identities = 38/113 (33%), Positives = 63/113 (55%), Gaps = 1/113 (0%)
Frame = +3
Query: 291 LHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHGYPMTLSELTSALPIHPTKSHSVYR 470
+ AQAH+W I+ F +S+ L+ A++LGI DII+ + PM L++L S LP+ ++YR
Sbjct: 12 IKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDNLYR 71
Query: 471 LMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPYLTAMLDPVF 629
++R LV +K S ++ Y L + LL ++ S+ P + M F
Sbjct: 72 ILRYLVKMEILRVEK-SDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQKDF 123
Database: UniProt/Swiss-Prot
Posted date: Thu Apr 23 14:22:33 2009
Number of letters in database: 163,773,382
Number of sequences in database: 462,764
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,513,639,154
Number of Sequences: 462764
Number of Extensions: 12513639154
Number of Successful Extensions: 124453114
Number of sequences better than 0.0: 0
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