BLASTX 7.6.2
Query= RU26660 /QuerySize=2681
(2680 letters)
Database: UniProt/Swiss-Prot;
462,764 sequences; 163,773,382 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O65351|SUBL_ARATH Subtilisin-like protease OS=Arabidopsis tha... 920 1e-266
sp|Q9LLL8|XSP1_ARATH Xylem serine proteinase 1 OS=Arabidopsis th... 421 1e-116
sp|Q39547|CUCM1_CUCME Cucumisin OS=Cucumis melo PE=1 SV=1 358 2e-097
sp|P29141|SUBV_BACSU Minor extracellular protease vpr OS=Bacillu... 87 5e-016
sp|P00782|SUBT_BACAM Subtilisin BPN' OS=Bacillus amyloliquefacie... 53 8e-006
>sp|O65351|SUBL_ARATH Subtilisin-like protease OS=Arabidopsis thaliana GN=ARA12
PE=1 SV=1
Length = 757
Score = 920 bits (2376), Expect = 1e-266
Identities = 453/750 (60%), Positives = 552/750 (73%), Gaps = 16/750 (2%)
Frame = -3
Query: 2549 CEVVAEESEHVKKTYIIHMDKSQIPASFDDDHVNWYGSSLKAVSDSADMLYTYKTVIHGY 2370
C V + S+ + TYI+HM KSQ+P+SF D H NWY SSL+++SDSA++LYTY+ IHG+
Sbjct: 20 CHVSSSSSD--QGTYIVHMAKSQMPSSF-DLHSNWYDSSLRSISDSAELLYTYENAIHGF 76
Query: 2369 STRLTAEEAEQLEKQPGILSVLPERRYELHTTRTPEFLGLRKSEA-VLPASDKMGEVIIG 2193
STRLT EEA+ L QPG++SVLPE RYELHTTRTP FLGL + A + P + +V++G
Sbjct: 77 STRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVG 136
Query: 2192 VVDTGVWPELKSYDDTGFGPVPTGWKGSCQQGKTFNSSSCNKKLIGAAFFSNGYEAAFGP 2013
V+DTGVWPE KSY D GFGP+P+ WKG C+ G F +S CN+KLIGA FF+ GYE+ GP
Sbjct: 137 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 196
Query: 2012 VDENVESKSPRXXXXXXXXXXXXXXXXXXXXXXFLGYAPGKARGMAPQARVATYKACWLG 1833
+DE+ ES+SPR LGYA G ARGMAP+ARVA YK CWLG
Sbjct: 197 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLG 256
Query: 1832 GCFGSDIIAAMEKAVEDGVDVLSLSIGGSQSDYYRDTVAIGAFSAAAQGILVSCSAGNGG 1653
GCF SDI+AA++KA+ D V+VLS+S+GG SDYYRD VAIGAF+A +GILVSCSAGN G
Sbjct: 257 GCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAG 316
Query: 1652 PEAGSLSNVAPWITTVGAGTLDRDFPAYVSLGNAKKFRGISLYRGTALSSGLIPLVDARN 1473
P + SLSNVAPWITTVGAGTLDRDFPA LGN K F G+SL++G AL L+P + A N
Sbjct: 317 PSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGN 376
Query: 1472 VXXXXXXXX-XXXXXIPAKVAGKIVVCDRGGTPRVQKSLVVKKAGGVGMILTNTETYGEE 1296
IP KV GKIV+CDRG RVQK VVK AGGVGMIL NT GEE
Sbjct: 377 ASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEE 436
Query: 1295 LVADAYFLPTAAVGQKAGDAIKSYIASDSNPTATISLGETEVGVQPSPVVAAFSSRGPNS 1116
LVADA+ LP VG+KAGD I+ Y+ +D NPTA+IS+ T VGV+PSPVVAAFSSRGPNS
Sbjct: 437 LVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNS 496
Query: 1115 VTPEVLKPDLIAPGMNILAGWTGAVGPTGLPEDKRSVVFNIISGTSMSCPHVSGLAALVK 936
+TP +LKPDLIAPG+NILA WTGA GPTGL D R V FNIISGTSMSCPHVSGLAAL+K
Sbjct: 497 ITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLK 556
Query: 935 AAHPDWSPAAIKSALMTTAYSNYKTGETIEDVSTGSAATPFDYGAGHVDPVAALDPGLVY 756
+ HP+WSPAAI+SALMTTAY YK G+ + D++TG +TPFD+GAGHV P A +PGL+Y
Sbjct: 557 SVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 616
Query: 755 DLAVEDYLSFLCALNYSASKIKQTTTKDFTCDSSKKYSLGNLNYPSFSLALDXXXXXXXX 576
DL EDYL FLCALNY++ +I+ + +++TCD SK YS+ +LNYPSF++ +D
Sbjct: 617 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVD-------- 668
Query: 575 XXXXXXLKYTRTLTNVGAPATYKVSMSSLTRSVKMSVEPESLSFSDAYEKKTYTVTF-VA 399
KYTRT+T+VG TY V ++S T VK+SVEP L+F +A EKK+YTVTF V
Sbjct: 669 --GVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVD 726
Query: 398 SSLPSGTASFGRLEWSDGKHIVGSPIAYTW 309
SS PSG+ SFG +EWSDGKH+VGSP+A +W
Sbjct: 727 SSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
>sp|Q9LLL8|XSP1_ARATH Xylem serine proteinase 1 OS=Arabidopsis thaliana GN=XSP1
PE=2 SV=1
Length = 749
Score = 421 bits (1082), Expect = 1e-116
Identities = 270/749 (36%), Positives = 394/749 (52%), Gaps = 49/749 (6%)
Frame = -3
Query: 2531 ESEHVKKTYIIHM-DKSQIPASFDDDHVNWYGSSLKAVSDSAD--MLYTYKTVIHGYSTR 2361
E EH K YII++ D+ H+N SSL + A +Y+Y + ++ +
Sbjct: 30 EDEHAKDFYIIYLGDRPDNTEETIKTHINLL-SSLNISQEEAKERKVYSYTKAFNAFAAK 88
Query: 2360 LTAEEAEQLEKQPGILSVLPERRYELHTTRTPEFLGLRKSEAVLPASDKMGEVIIGVVDT 2181
L+ EA+++ + ++SV + +LHTT++ +F+GL + +++ +VIIGV+DT
Sbjct: 89 LSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAER--DVIIGVLDT 146
Query: 2180 GVWPELKSYDDTGFGPVPTGWKGSCQQGKTFNSSSCNKKLIGAAFFSNGYEAAFGPVDEN 2001
G+ P+ +S+ D G GP P WKGSC K F + CN K+IGA +F + G
Sbjct: 147 GITPDSESFLDHGLGPPPAKWKGSCGPYKNF--TGCNNKIIGAKYFKHDGNVPAG----- 199
Query: 2000 VESKSPRXXXXXXXXXXXXXXXXXXXXXXFLGYAPGKARGMAPQARVATYKACWL-GGCF 1824
E +SP G A G ARG P AR+A YK CW GC
Sbjct: 200 -EVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCA 258
Query: 1823 GSDIIAAMEKAVEDGVDVLSLSIGGSQSDYYRDTVAIGAFSAAAQGILVSCSAGNGGPEA 1644
DI+A E A+ DGV+++S+SIGG +DY D++++G+F A +GIL SAGN GP +
Sbjct: 259 DMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSS 318
Query: 1643 GSLSNVAPWITTVGAGTLDRDFPAYVSLGNAKKF--RGISLYRGTALSSGLIPLVD-ARN 1473
G+++N PWI TV A +DR F + + LGN K F GIS++ A S L+ VD A+N
Sbjct: 319 GTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKN 378
Query: 1472 VXXXXXXXXXXXXXIP-AKVAGKIVVCDRGGTPRVQKSLVVKKAGGVGMILTNTETYGEE 1296
+ KV GK++VC GG +K GG G I+ + +
Sbjct: 379 TDDKYLARYCFSDSLDRKKVKGKVMVCRMGGG---GVESTIKSYGGAGAIIVSDQYLDN- 434
Query: 1295 LVADAYFLPTAAVGQKAGDAIKSYIASDSNPTATISLGETEVGVQPSPVVAAFSSRGPNS 1116
A + P +V GD I YI S + +A I +T P+P VA+FSSRGPN
Sbjct: 435 --AQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQ--KTRQVTIPAPFVASFSSRGPNP 490
Query: 1115 VTPEVLKPDLIAPGMNILAGWTGAVGPTGLPEDKRSVVFNIISGTSMSCPHVSGLAALVK 936
+ +LKPD+ APG++ILA +T TGL D + F I+SGTSM+CPHV+G+AA VK
Sbjct: 491 GSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVK 550
Query: 935 AAHPDWSPAAIKSALMTTAYS-NYKTGETIEDVSTGSAATPFDYGAGHVDPVAALDPGLV 759
+ HPDW+PAAIKSA++T+A + + + E F YG G ++P A PGLV
Sbjct: 551 SFHPDWTPAAIKSAIITSAKPISRRVNKDAE----------FAYGGGQINPRRAASPGLV 600
Query: 758 YDLAVEDYLSFLCALNYSASKIKQTT-TKDFTCDSSKKYSLG--NLNYPSFSLALDXXXX 588
YD+ Y+ FLC Y+A+ + T+ +C SS LG +LNYP+ L L
Sbjct: 601 YDMDDISYVQFLCGEGYNATTLAPLVGTRSVSC-SSIVPGLGHDSLNYPTIQLTLRSAKT 659
Query: 587 XXXXXXXXXXLKYTRTLTNVGAPATYKVSMSSLTRSVKMSVEPESLSFSDAYEKKTYTVT 408
+ R +TNVG P++ + + V+++VEP+SLSFS A +K+++ V
Sbjct: 660 STLAV-------FRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVV 712
Query: 407 FVASSLPSGTASFGRLEWSDGKHIVGSPI 321
A + G G L W +H V SPI
Sbjct: 713 VKAKQMTPGKIVSGLLVWKSPRHSVRSPI 741
>sp|Q39547|CUCM1_CUCME Cucumisin OS=Cucumis melo PE=1 SV=1
Length = 731
Score = 358 bits (917), Expect = 2e-097
Identities = 218/555 (39%), Positives = 304/555 (54%), Gaps = 36/555 (6%)
Frame = -3
Query: 2516 KKTYIIHMD-KSQIPASFDDDHVNWYGSSLKAVSDSADMLYTYKTVIHGYSTRLTAEEAE 2340
K YI++M K + P S H + + +L+TYK +G++ +LT EEAE
Sbjct: 31 KNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAE 90
Query: 2339 QLEKQPGILSVLPERRYELHTTRTPEFLGLRKSEAVLPASDKMGEVIIGVVDTGVWPELK 2160
++ G++SV ELHTTR+ +FLG + V S +++GV+DTG+WPE
Sbjct: 91 KIASMEGVVSVFLNEMNELHTTRSWDFLGFPLT--VPRRSQVESNIVVGVLDTGIWPESP 148
Query: 2159 SYDDTGFGPVPTGWKGSCQQGKTFNSSSCNKKLIGAAFFSNGYEAAFGPVDENVESKSPR 1980
S+DD GF P P WKG+C+ T N+ CN+K+IGA + G + G V+ PR
Sbjct: 149 SFDDEGFSPPPPKWKGTCE---TSNNFRCNRKIIGARSYHIGRPISPGDVN------GPR 199
Query: 1979 XXXXXXXXXXXXXXXXXXXXXXFLGYAPGKARGMAPQARVATYKACWLGGCFGSDIIAAM 1800
G G ARG P AR+A YK CW GC +DI+AA
Sbjct: 200 DTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAY 259
Query: 1799 EKAVEDGVDVLSLSIGGSQ-SDYYRDTVAIGAFSAAAQGILVSCSAGNGGPEAGSLSNVA 1623
+ A+ DGVD++SLS+GG+ Y+ D +AIG+F A +GIL S SAGNGGP + ++++
Sbjct: 260 DDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLS 319
Query: 1622 PWITTVGAGTLDRDFPAYVSLGNAKKFRGISLYRGTALSSGLIPLVDARNV-----XXXX 1458
PW+ +V A T+DR F V +GN + F+G+S+ + PLV R++
Sbjct: 320 PWLLSVAASTMDRKFVTQVQIGNGQSFQGVSI---NTFDNQYYPLVSGRDIPNTGFDKST 376
Query: 1457 XXXXXXXXXIPAKVAGKIVVCDRG-GTPRVQKSLVVKKAGGVGMILT-NTETYGEELVAD 1284
P + GKIVVC+ G KSL G G+++T NT Y AD
Sbjct: 377 SRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSL----DGAAGVLMTSNTRDY-----AD 427
Query: 1283 AYFLPTAAVGQKAGDAIKSYIASDSNPTATISLGETEVGVQPSPVVAAFSSRGPNSVTPE 1104
+Y LP++ + A YI S +P ATI T + +PVV +FSSRGPN T +
Sbjct: 428 SYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNAS-APVVVSFSSRGPNRATKD 486
Query: 1103 VLKPDLIAPGMNILAGWTGAVGPTGLPEDKRSVVFNIISGTSMSCPHVSGLAALVKAAHP 924
V+KPD+ PG+ ILA W +V P G +R+ +FNIISGTSMSCPH++G+A VK +P
Sbjct: 487 VIKPDISGPGVEILAAWP-SVAPVG--GIRRNTLFNIISGTSMSCPHITGIATYVKTYNP 543
Query: 923 DWSPAAIKSALMTTA 879
WSPAAIKSALMTTA
Sbjct: 544 TWSPAAIKSALMTTA 558
Score = 67 bits (163), Expect = 4e-010
Identities = 30/71 (42%), Positives = 43/71 (60%)
Frame = -3
Query: 815 FDYGAGHVDPVAALDPGLVYDLAVEDYLSFLCALNYSASKIKQTTTKDFTCDSSKKYSLG 636
F YG+GHV+P+ A+ PGLVYD DY+ FLC Y+ +++ T C S +
Sbjct: 571 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVW 630
Query: 635 NLNYPSFSLAL 603
+LNYPSF L++
Sbjct: 631 DLNYPSFGLSV 641
>sp|P29141|SUBV_BACSU Minor extracellular protease vpr OS=Bacillus subtilis
GN=vpr PE=1 SV=1
Length = 806
Score = 87 bits (214), Expect = 5e-016
Identities = 61/180 (33%), Positives = 91/180 (50%), Gaps = 12/180 (6%)
Frame = -3
Query: 1418 VAGKIVVCDRGGTPRVQKSLVVKKAGGVGMILTNTETYGEELVADAYFLPTAAVGQKAGD 1239
+ GK+ V RG V K+ KKAG +GM++ N + E +PT + + G+
Sbjct: 400 LTGKVAVVKRGSIAFVDKADNAKKAGAIGMVVYNNLSGEIEANVPGMSVPTIKLSLEDGE 459
Query: 1238 AIKSYIASDSNPTATISLGETEVGVQPSPVVAAFSSRGPNSVTPEVLKPDLIAPGMNILA 1059
+ S + + T +G Q VA FSSRGP T ++KPD+ APG+NI+
Sbjct: 460 KLVSALKAGETKTTFKLTVSKALGEQ----VADFSSRGPVMDT-WMIKPDISAPGVNIV- 513
Query: 1058 GWTGAVGPTGLPEDKRSVVFNIISGTSMSCPHVSGLAALVKAAHPDWSPAAIKSALMTTA 879
+ PT P+ + GTSM+ PH++G A++K A P WS IK+A+M TA
Sbjct: 514 ----STIPTHDPDHPYG--YGSKQGTSMASPHIAGAVAVIKQAKPKWSVEQIKAAIMNTA 567
>sp|P00782|SUBT_BACAM Subtilisin BPN' OS=Bacillus amyloliquefaciens GN=apr PE=1
SV=1
Length = 382
Score = 53 bits (126), Expect = 8e-006
Identities = 39/128 (30%), Positives = 63/128 (49%), Gaps = 25/128 (19%)
Frame = -3
Query: 1265 AAVGQKAGDAIKSYIASDSNPTATISLGETEVGVQPSPVVAAFSSRGPNSVTPEVLKPDL 1086
AA G + S + + I++G V S A+FSS GP + D+
Sbjct: 258 AAAGNEGTSGSSSTVGYPGKYPSVIAVG----AVDSSNQRASFSSVGP--------ELDV 305
Query: 1085 IAPGMNILAGWTGAVGPTGLPEDKRSVVFNIISGTSMSCPHVSGLAALVKAAHPDWSPAA 906
+APG++I + LP +K + +GTSM+ PHV+G AAL+ + HP+W+
Sbjct: 306 MAPGVSI---------QSTLPGNK----YGAYNGTSMASPHVAGAAALILSKHPNWTNTQ 352
Query: 905 IKSALMTT 882
++S+L T
Sbjct: 353 VRSSLENT 360
Database: UniProt/Swiss-Prot
Posted date: Thu Apr 23 14:22:33 2009
Number of letters in database: 163,773,382
Number of sequences in database: 462,764
Lambda K H
0.267 0.041 0.140
Gapped
Lambda K H
0.267 0.041 0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,041,410,375
Number of Sequences: 462764
Number of Extensions: 29041410375
Number of Successful Extensions: 295390318
Number of sequences better than 0.0: 0
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