Blast details for RU07773 (TrEMBL)


BLASTX 7.6.2

Query= RU07773 /QuerySize=222
        (221 letters)

Database: UniProt/TrEMBL;
          7,695,149 sequences; 2,506,224,640 total letters
                                                                  Score    E
Sequences producing significant alignments:                       (bits) Value

tr|A5B6T5|A5B6T5_VITVI Putative uncharacterized protein OS=Vitis...     89   1e-016
tr|A7PV47|A7PV47_VITVI Chromosome chr4 scaffold_32, whole genome...     89   1e-016
tr|B9RM55|B9RM55_RICCO Putative uncharacterized protein OS=Ricin...     88   1e-016
tr|B9SMP1|B9SMP1_RICCO Putative uncharacterized protein OS=Ricin...     88   2e-016
tr|A5BBX0|A5BBX0_VITVI Putative uncharacterized protein OS=Vitis...     86   7e-016
tr|A7QYY8|A7QYY8_VITVI Chromosome undetermined scaffold_259, who...     86   7e-016
tr|A9NU94|A9NU94_PICSI Putative uncharacterized protein OS=Picea...     84   3e-015
tr|B9GNH0|B9GNH0_POPTR Predicted protein OS=Populus trichocarpa ...     84   3e-015
tr|B9IB30|B9IB30_POPTR Predicted protein OS=Populus trichocarpa ...     84   3e-015
tr|A9NSC8|A9NSC8_PICSI Putative uncharacterized protein OS=Picea...     83   5e-015
tr|Q94AG1|Q94AG1_ARATH AT3g07470/F21O3_18 OS=Arabidopsis thalian...     82   1e-014
tr|Q9SRR9|Q9SRR9_ARATH F21O3.18 protein OS=Arabidopsis thaliana ...     82   1e-014
tr|Q53HY4|Q53HY4_TOBAC Putative uncharacterized protein mc304 OS...     79   9e-014
tr|B8A845|B8A845_ORYSI Putative uncharacterized protein OS=Oryza...     77   3e-013
tr|Q5N7W9|Q5N7W9_ORYSJ Putative uncharacterized protein OS=Oryza...     77   3e-013
tr|Q3EBA8|Q3EBA8_ARATH Uncharacterized protein At3g07460.2 OS=Ar...     76   7e-013
tr|Q8L7K5|Q8L7K5_ARATH Putative uncharacterized protein At3g0746...     76   7e-013
tr|Q8LCX5|Q8LCX5_ARATH Putative uncharacterized protein OS=Arabi...     76   7e-013
tr|Q9SRS0|Q9SRS0_ARATH F21O3.17 protein OS=Arabidopsis thaliana ...     76   7e-013
tr|Q8LFQ4|Q8LFQ4_ARATH Putative uncharacterized protein OS=Arabi...     75   2e-012
tr|Q8VZN3|Q8VZN3_ARATH Putative uncharacterized protein At5g1638...     75   2e-012
tr|Q9FFE5|Q9FFE5_ARATH Gb|AAF02163.1 OS=Arabidopsis thaliana PE=...     75   2e-012
tr|A7P137|A7P137_VITVI Chromosome chr19 scaffold_4, whole genome...     66   6e-010
tr|B6TFE5|B6TFE5_MAIZE Putative uncharacterized protein OS=Zea m...     66   8e-010
tr|B9I060|B9I060_POPTR Predicted protein OS=Populus trichocarpa ...     65   1e-009
tr|B9RXW9|B9RXW9_RICCO Putative uncharacterized protein OS=Ricin...     65   1e-009
tr|A9P8A3|A9P8A3_POPTR Putative uncharacterized protein OS=Popul...     65   2e-009
tr|B9I7D3|B9I7D3_POPTR Predicted protein OS=Populus trichocarpa ...     65   2e-009
tr|Q0WLF7|Q0WLF7_ARATH At1g61667 OS=Arabidopsis thaliana PE=2 SV=1      64   3e-009
tr|Q8LDT1|Q8LDT1_ARATH Putative uncharacterized protein OS=Arabi...     64   3e-009

>tr|A5B6T5|A5B6T5_VITVI Putative uncharacterized protein OS=Vitis vinifera
        GN=VITISV_040145 PE=4 SV=1

          Length = 192

 Score =  89 bits (218), Expect = 1e-016
 Identities = 40/43 (93%), Positives = 42/43 (97%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCT 93
           LFLWFPVKGIRVD+PSSGLIYFDVGV+ KQFSLSLFESPPDCT
Sbjct: 107 LFLWFPVKGIRVDIPSSGLIYFDVGVIYKQFSLSLFESPPDCT 149

>tr|A7PV47|A7PV47_VITVI Chromosome chr4 scaffold_32, whole genome shotgun
        sequence OS=Vitis vinifera GN=GSVIVT00025047001 PE=4 SV=1

          Length = 192

 Score =  89 bits (218), Expect = 1e-016
 Identities = 40/43 (93%), Positives = 42/43 (97%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCT 93
           LFLWFPVKGIRVD+PSSGLIYFDVGV+ KQFSLSLFESPPDCT
Sbjct: 107 LFLWFPVKGIRVDIPSSGLIYFDVGVIYKQFSLSLFESPPDCT 149

>tr|B9RM55|B9RM55_RICCO Putative uncharacterized protein OS=Ricinus communis
        GN=RCOM_1077750 PE=4 SV=1

          Length = 184

 Score =  88 bits (217), Expect = 1e-016
 Identities = 41/45 (91%), Positives = 44/45 (97%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFE+P +CTA+
Sbjct: 107 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFENPIECTAV 151

>tr|B9SMP1|B9SMP1_RICCO Putative uncharacterized protein OS=Ricinus communis
        GN=RCOM_1626990 PE=4 SV=1

          Length = 186

 Score =  88 bits (216), Expect = 2e-016
 Identities = 41/45 (91%), Positives = 43/45 (95%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQFSLSLFE+P DCTA+
Sbjct: 102 LFLWFPVKGIRVDVPSSGLIYFDVGVVSKQFSLSLFETPRDCTAV 146

>tr|A5BBX0|A5BBX0_VITVI Putative uncharacterized protein OS=Vitis vinifera
        GN=VITISV_016695 PE=4 SV=1

          Length = 228

 Score =  86 bits (211), Expect = 7e-016
 Identities = 40/45 (88%), Positives = 43/45 (95%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQFSLSLFE+PP+C A+
Sbjct: 102 LFLWFPVKGIRVDVPSSGLIYFDVGVVFKQFSLSLFETPPNCKAV 146

>tr|A7QYY8|A7QYY8_VITVI Chromosome undetermined scaffold_259, whole genome
        shotgun sequence OS=Vitis vinifera GN=GSVIVT00010403001 PE=4 SV=1

          Length = 160

 Score =  86 bits (211), Expect = 7e-016
 Identities = 40/45 (88%), Positives = 43/45 (95%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQFSLSLFE+PP+C A+
Sbjct: 102 LFLWFPVKGIRVDVPSSGLIYFDVGVVFKQFSLSLFETPPNCKAV 146

>tr|A9NU94|A9NU94_PICSI Putative uncharacterized protein OS=Picea sitchensis
        PE=2 SV=1

          Length = 188

 Score =  84 bits (206), Expect = 3e-015
 Identities = 38/42 (90%), Positives = 41/42 (97%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLWFPVKGIRVD+PSSGLIYFDVGVV KQ+SLSLFE+PPDC
Sbjct: 105 LFLWFPVKGIRVDIPSSGLIYFDVGVVYKQWSLSLFENPPDC 146

>tr|B9GNH0|B9GNH0_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_553126 PE=4 SV=1

          Length = 181

 Score =  84 bits (206), Expect = 3e-015
 Identities = 40/45 (88%), Positives = 41/45 (91%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQFSLSLFE P DC A+
Sbjct:  97 LFLWFPVKGIRVDVPSSGLIYFDVGVVFKQFSLSLFEMPRDCVAV 141

>tr|B9IB30|B9IB30_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_573209 PE=4 SV=1

          Length = 165

 Score =  84 bits (206), Expect = 3e-015
 Identities = 40/45 (88%), Positives = 41/45 (91%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQFSLSLFE P DC A+
Sbjct: 100 LFLWFPVKGIRVDVPSSGLIYFDVGVVFKQFSLSLFEMPRDCVAV 144

>tr|A9NSC8|A9NSC8_PICSI Putative uncharacterized protein OS=Picea sitchensis
        PE=2 SV=1

          Length = 236

 Score =  83 bits (204), Expect = 5e-015
 Identities = 37/42 (88%), Positives = 40/42 (95%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLWFPVKGIRVD+P+SG IYFDVGVV KQFSLSLFE+PPDC
Sbjct: 146 LFLWFPVKGIRVDIPNSGFIYFDVGVVYKQFSLSLFETPPDC 187

>tr|Q94AG1|Q94AG1_ARATH AT3g07470/F21O3_18 OS=Arabidopsis thaliana GN=At3g07470
        PE=2 SV=1

          Length = 169

 Score =  82 bits (201), Expect = 1e-014
 Identities = 38/42 (90%), Positives = 40/42 (95%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQ+SLSLFE+P DC
Sbjct: 103 LFLWFPVKGIRVDVPSSGLIYFDVGVVRKQYSLSLFETPRDC 144

>tr|Q9SRR9|Q9SRR9_ARATH F21O3.18 protein OS=Arabidopsis thaliana GN=F21O3.18
        PE=4 SV=1

          Length = 160

 Score =  82 bits (201), Expect = 1e-014
 Identities = 38/42 (90%), Positives = 40/42 (95%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLWFPVKGIRVDVPSSGLIYFDVGVV KQ+SLSLFE+P DC
Sbjct: 103 LFLWFPVKGIRVDVPSSGLIYFDVGVVRKQYSLSLFETPRDC 144

>tr|Q53HY4|Q53HY4_TOBAC Putative uncharacterized protein mc304 OS=Nicotiana
        tabacum GN=mc304 PE=2 SV=1

          Length = 220

 Score =  79 bits (193), Expect = 9e-014
 Identities = 36/45 (80%), Positives = 40/45 (88%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLWFPVK IRVD+PSSGLIYF+VGVV KQFSLS FE+P DCT +
Sbjct:  91 LFLWFPVKDIRVDIPSSGLIYFNVGVVSKQFSLSSFETPRDCTVV 135

>tr|B8A845|B8A845_ORYSI Putative uncharacterized protein OS=Oryza sativa subsp.
        indica GN=OsI_04890 PE=4 SV=1

          Length = 171

 Score =  77 bits (188), Expect = 3e-013
 Identities = 34/43 (79%), Positives = 39/43 (90%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCT 93
           LFLWFPV+GIRVDVPSSG+IYFDVGVV K F L++FE+PP CT
Sbjct: 105 LFLWFPVRGIRVDVPSSGVIYFDVGVVFKHFPLAVFEAPPPCT 147

>tr|Q5N7W9|Q5N7W9_ORYSJ Putative uncharacterized protein OS=Oryza sativa subsp.
        japonica GN=P0470A12.38 PE=2 SV=1

          Length = 171

 Score =  77 bits (188), Expect = 3e-013
 Identities = 34/43 (79%), Positives = 39/43 (90%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCT 93
           LFLWFPV+GIRVDVPSSG+IYFDVGVV K F L++FE+PP CT
Sbjct: 105 LFLWFPVRGIRVDVPSSGVIYFDVGVVFKHFPLAVFEAPPPCT 147

>tr|Q3EBA8|Q3EBA8_ARATH Uncharacterized protein At3g07460.2 OS=Arabidopsis
        thaliana GN=At3g07460 PE=4 SV=1

          Length = 271

 Score =  76 bits (185), Expect = 7e-013
 Identities = 35/45 (77%), Positives = 40/45 (88%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLW  VKGIRVDVPSSGLI+FDVGV+ KQ+SLSLFE+P DC A+
Sbjct: 102 LFLWLQVKGIRVDVPSSGLIFFDVGVLRKQYSLSLFETPRDCVAV 146

>tr|Q8L7K5|Q8L7K5_ARATH Putative uncharacterized protein At3g07460
        OS=Arabidopsis thaliana GN=At3g07460 PE=2 SV=1

          Length = 177

 Score =  76 bits (185), Expect = 7e-013
 Identities = 35/45 (77%), Positives = 40/45 (88%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLW  VKGIRVDVPSSGLI+FDVGV+ KQ+SLSLFE+P DC A+
Sbjct: 102 LFLWLQVKGIRVDVPSSGLIFFDVGVLRKQYSLSLFETPRDCVAV 146

>tr|Q8LCX5|Q8LCX5_ARATH Putative uncharacterized protein OS=Arabidopsis thaliana
        PE=2 SV=1

          Length = 177

 Score =  76 bits (185), Expect = 7e-013
 Identities = 35/45 (77%), Positives = 40/45 (88%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLW  VKGIRVDVPSSGLI+FDVGV+ KQ+SLSLFE+P DC A+
Sbjct: 102 LFLWLQVKGIRVDVPSSGLIFFDVGVLRKQYSLSLFETPRDCVAV 146

>tr|Q9SRS0|Q9SRS0_ARATH F21O3.17 protein OS=Arabidopsis thaliana GN=F21O3.17
        PE=4 SV=1

          Length = 158

 Score =  76 bits (185), Expect = 7e-013
 Identities = 35/45 (77%), Positives = 40/45 (88%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTAL 87
           LFLW  VKGIRVDVPSSGLI+FDVGV+ KQ+SLSLFE+P DC A+
Sbjct: 102 LFLWLQVKGIRVDVPSSGLIFFDVGVLRKQYSLSLFETPRDCVAV 146

>tr|Q8LFQ4|Q8LFQ4_ARATH Putative uncharacterized protein OS=Arabidopsis thaliana
        PE=2 SV=1

          Length = 195

 Score =  75 bits (182), Expect = 2e-012
 Identities = 36/44 (81%), Positives = 37/44 (84%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTA 90
           LFLWF VKGI VD  SSGLI+FDVGV DKQ SLSLFESP DCTA
Sbjct: 103 LFLWFAVKGIHVDPQSSGLIHFDVGVADKQLSLSLFESPRDCTA 146

>tr|Q8VZN3|Q8VZN3_ARATH Putative uncharacterized protein At5g16380
        OS=Arabidopsis thaliana GN=At5g16380 PE=2 SV=1

          Length = 195

 Score =  75 bits (182), Expect = 2e-012
 Identities = 36/44 (81%), Positives = 37/44 (84%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTA 90
           LFLWF VKGI VD  SSGLI+FDVGV DKQ SLSLFESP DCTA
Sbjct: 103 LFLWFAVKGIHVDPQSSGLIHFDVGVADKQLSLSLFESPRDCTA 146

>tr|Q9FFE5|Q9FFE5_ARATH Gb|AAF02163.1 OS=Arabidopsis thaliana PE=4 SV=1

          Length = 175

 Score =  75 bits (182), Expect = 2e-012
 Identities = 36/44 (81%), Positives = 37/44 (84%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCTA 90
           LFLWF VKGI VD  SSGLI+FDVGV DKQ SLSLFESP DCTA
Sbjct: 103 LFLWFAVKGIHVDPQSSGLIHFDVGVADKQLSLSLFESPRDCTA 146

>tr|A7P137|A7P137_VITVI Chromosome chr19 scaffold_4, whole genome shotgun
        sequence OS=Vitis vinifera GN=GSVIVT00027470001 PE=4 SV=1

          Length = 154

 Score =  66 bits (160), Expect = 6e-010
 Identities = 30/42 (71%), Positives = 33/42 (78%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLW PVK I VD P+SGLI FD+G+  KQ SLSLFE PPDC
Sbjct:  93 LFLWLPVKDIIVDDPNSGLILFDIGLAHKQLSLSLFEDPPDC 134

>tr|B6TFE5|B6TFE5_MAIZE Putative uncharacterized protein OS=Zea mays PE=2 SV=1

          Length = 179

 Score =  66 bits (159), Expect = 8e-010
 Identities = 27/43 (62%), Positives = 36/43 (83%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDCT 93
           LF+WFPV  I+VD+PSSG+IYF+VGVV K   L++F++PP CT
Sbjct: 109 LFMWFPVHDIKVDIPSSGVIYFNVGVVKKHLPLAVFDAPPACT 151

>tr|B9I060|B9I060_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_569042 PE=4 SV=1

          Length = 152

 Score =  65 bits (158), Expect = 1e-009
 Identities = 30/42 (71%), Positives = 32/42 (76%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLW PVK I VD P SGLI FD+GV  KQ SLSLFE PP+C
Sbjct: 103 LFLWLPVKDITVDDPRSGLILFDIGVAHKQLSLSLFEDPPNC 144

>tr|B9RXW9|B9RXW9_RICCO Putative uncharacterized protein OS=Ricinus communis
        GN=RCOM_0906790 PE=4 SV=1

          Length = 161

 Score =  65 bits (158), Expect = 1e-009
 Identities = 30/42 (71%), Positives = 33/42 (78%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLWFPVKGI V+ PSSGLI FD+G+  KQ S SLFE PP C
Sbjct: 100 LFLWFPVKGIMVNDPSSGLILFDIGLAHKQLSRSLFEDPPVC 141

>tr|A9P8A3|A9P8A3_POPTR Putative uncharacterized protein OS=Populus trichocarpa
        PE=2 SV=1

          Length = 165

 Score =  65 bits (156), Expect = 2e-009
 Identities = 30/42 (71%), Positives = 31/42 (73%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLW PVK I VD P SGLI FD+GV  KQ SLSLFE PP C
Sbjct: 103 LFLWLPVKDIIVDDPGSGLILFDIGVAHKQLSLSLFEDPPSC 144

>tr|B9I7D3|B9I7D3_POPTR Predicted protein OS=Populus trichocarpa
        GN=POPTRDRAFT_823760 PE=4 SV=1

          Length = 165

 Score =  65 bits (156), Expect = 2e-009
 Identities = 30/42 (71%), Positives = 31/42 (73%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLW PVK I VD P SGLI FD+GV  KQ SLSLFE PP C
Sbjct: 103 LFLWLPVKDIIVDDPGSGLILFDIGVAHKQLSLSLFEDPPSC 144

>tr|Q0WLF7|Q0WLF7_ARATH At1g61667 OS=Arabidopsis thaliana PE=2 SV=1

          Length = 156

 Score =  64 bits (154), Expect = 3e-009
 Identities = 29/42 (69%), Positives = 32/42 (76%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLW PVKGI V+ PSSGL+ FD+GV  KQ S SLFE PP C
Sbjct:  94 LFLWLPVKGIAVNDPSSGLVLFDIGVAHKQISRSLFEDPPVC 135

>tr|Q8LDT1|Q8LDT1_ARATH Putative uncharacterized protein OS=Arabidopsis thaliana
        PE=2 SV=1

          Length = 157

 Score =  64 bits (154), Expect = 3e-009
 Identities = 29/42 (69%), Positives = 32/42 (76%)
 Frame = -1

Query: 221 LFLWFPVKGIRVDVPSSGLIYFDVGVVDKQFSLSLFESPPDC 96
           LFLW PVKGI V+ PSSGL+ FD+GV  KQ S SLFE PP C
Sbjct:  95 LFLWLPVKGIAVNDPSSGLVLFDIGVAHKQISRSLFEDPPVC 136

  Database: UniProt/TrEMBL
    Posted date:  Sun May 10 14:41:50 2009
  Number of letters in database: 2,506,224,640
  Number of sequences in database:  7,695,149

Lambda     K     H
   0.267   0.041    0.140
Gapped
Lambda     K     H
   0.267   0.041    0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 193,545,446,174
Number of Sequences: 7695149
Number of Extensions: 193545446174
Number of Successful Extensions: 162757913
Number of sequences better than 0.0: 0